• Journal of Plant Biology
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• 제38권4호
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• pp.381-388
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• 1995

해녀콩(Canavalia lineata) 뿌리혹으로부터 만들어진 cDNA library를 뿌리의 총 RNA, leghemoglobin 및 uricasell cDNA를 competitor로 사용하여 차등 혼성화반응으로 후기 뿌리혹에서 발현되며 Cno이, Cne2, Cne3, Clb2로 명명된 4개의 cDNA 클론을 얻었다. Cnod1은 벼의 cysteine proteinase를 암호화하는 유전자와 유사한데 1450nt의 전사체외 혼성화 반응을 보였으며 뿌리혹에서만 발현되고 뿌리혹 발달 후기에서 가장 높은 발현을 보였다. Cne2는 벼의 Expressed Sequence Tag의 한 종류와 유사하고 900 nt의 전사체와 혼성화 반응을 보였으며 뿌리혹에서 주로 발현되었다. Cne2는 접종 후 13일째 발현이 증폭된 후 일정하게 유지되었다. Cne2는 완두의 tonoplast 막 내재 단백질 TRG31을 암호화하는 유전자와 유사하며 뿌리에서 가장 많이 발현되었다. Cne3는 1700 nt와 1400 nt의 전사체와 혼성화반응을 보였으며 뿌리혹의 성장, 발달과 일치하는 발현 양상을 보였다. Clb2는 800 nt의 전사체와 혼성화반응을 보였으며 접종 후 8일째부터 발현이 시작되어 13일째 증폭되고 그 이후 일정한 수준을 유지하였다.

• Asian Pacific Journal of Cancer Prevention
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• 제14권11호
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• pp.6315-6319
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• 2013

To study the differentiated expression of the proto-oncogene Pokemon in nasopharyngeal carcinoma (NPC) cell lines and tissues, mRNA and protein expression levels of CNE1, CNE2, CNE3 and C666-1 were detected separately by reverse transcription polymerase chain reaction (RT-PCR), real-time PCR and Western-blotting. The immortalized nasopharyngeal epithelial cell line NP69 was used as a control. The Pokemon protein expression level in biopsy specimens from chronic rhinitis patients and undifferentiated non keratinizing NPC patients was determined by Western-blotting and arranged from high to low: C666-1>CNE1>CNE2> CNE3>NP69. The Pokemon mRNA expression level was also arranged from high to low: CNE1>CNE2>NP69>C666-1>CNE3. Pokemon expression of NP69 and C666-1 obviously varied from mRNA to protein. The Pokemon protein level of NPC biopsy specimens was obviously higher than in chronic rhinitis. The data suggest that high Pokemon protein expression is closely associated with undifferentiated non-keratinizing NPC and may provide useful information for NPC molecular target therapy.

• Asian Pacific Journal of Cancer Prevention
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• 제15권17호
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• pp.7065-7068
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• 2014

To investigate the anti-proliferative mechanism of mangiferin in a human nasopharyngeal carcinoma cell line, CNE2 cells were incubated with different concentrations of mangiferin (12.5, 25, 50, 100, 150 and $200{\mu}M$) or with PBS as a control for 72 hours. Analyses were made of the cell cycle and apoptosis with measurement of mRNA and protein levels of two apoptosis-related genes, Bcl-2 and Bax. Flow cytometry assays showed mangiferin could inhibit CNE2 cell proliferation via G2/M arrest and induction of early apoptosis. Real time PCR and Western blotting showed the mRNA and protein level of Bcl-2 to be down-regulated, while those of Bax were upregulated, when CNE2 cells were treated with mangiferin. This investigation indicated anti-proliferation effects of mangiferin through induction of cell apoptosis regulated by Bcl-2 and Bax expression.

• 천문학논총
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• 제30권2호
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• pp.237-240
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• 2015

Classical novae (CNe) are interacting binary systems whose outbursts are powered by a thermonuclear runaway in accreted material onto the surface of a white dwarf (WD). The secondary star in such systems fills its Roche lobe and material is transferred onto the WD primary star via an accretion disk. Recurrent novae (RNe) show many similarities to CNe, but have had more than one recorded outburst. RNe play an important role as one of the suspected progenitor systems of Type Ia supernovae, which are used as primary distance indicators in cosmology. Thus, it is important to investigate the nature of their central binary systems to determine the relation between the parameters of the central system and the outburst type, and finally ascertain the population of novae that might be available to give rise to the progenitors of Type Ia SNe. A low outburst amplitude is adopted as a criterion that may help distinguish RNe from CNe and was therefore used to select targets for observations from ground-based observatories including the Liverpool Telescope and the Southern African Large Telescope as well as the full-sky space-based archive of the Solar Mass Ejection Imager (SMEI). We found that at least four objects currently classified as CNe are possibly RNe candidates based on their quiescent spectra. We also searched the SMEI archive for additional outbursts of bright CNe that might otherwise have been missed but did not find a conclusive example.

• Asian Pacific Journal of Cancer Prevention
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• 제15권11호
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• pp.4663-4670
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• 2014

Trichostatin A (TSA) is a histone deacetylase (HDAC) inhibitor. We here investigated its effects on proliferation and apoptosis of the CNE2 carcinoma cell line, and attempted to establish genome-wide DNA methylation alteration due to differentially histone acetylation status. After cells were treated by TSA, the inhibitory rate of cell proliferation was examined with a CCK8 kit, and cell apoptosis was determined by flow cytometry. Compared to control, TSA inhibited CNE2 cell growth and induced apoptosis. Furthermore, TSA was found to induce genome-wide methylation alteration as assessed by genome-wide methylation array. Overall DNA methylation level of cells treated with TSA was higher than in controls. Function and pathway analysis revealed that many genes with methylation alteration were involved in key biological roles, such as apoptosis and cell proliferation. Three genes (DAP3, HSPB1 and CLDN) were independently confirmed by quantitative real-time PCR. Finally, we conclude that TSA inhibits CNE2 cell growth and induces apoptosis in vitro involving genome-wide DNA methylation alteration, so that it has promising application prospects in treatment of NPC in vivo. Although many unreported hypermethylated/hypomethylated genes should be further analyzed and validated, the pointers to new biomarkers and therapeutic strategies in the treatment of NPC should be stressed.

• 디지털융복합연구
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• 제12권6호
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• pp.527-538
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• 2014

본 연구의 목적은 2008년에서 2012년에 대한간호협회 서울특별시간호사회에서 실시된 간호사 보수교육 자료를 분석하여 2012년 4월부터 전면시행 중인 의료인 면허신고제와 간호사 보수교육 운영의 발전을 위한 기초자료를 제공하고자 시도되었다. 2008년부터 2012년까지 5년간의 4,086건의 간호사 보수교육 프로그램을 이수한 165,759명의 자료를 SPSS 12.0을 이용하여 일반적 특성, 보수교육 프로그램, 면허취득경과기간을 기술통계, 면허취득경과기관에 따른 보수교육 프로그램의 차이를 t-Test, ANOVA, 상관관계로 분석하였다. 연구결과를 살펴보면, 2008년에 비해 2012년의 보수교육 이수자는 27.6% 증가하였고, 개발된 보수교육 프로그램수는 61.0% 증가하였다. 보수교육 이수자의 78.6%는 40세 미만이며, 89.5%가 병원에서 근무하고 있었다. 실시된 보수교육 내용 중 임상간호 내용이 65.6%로 가장 많았고, 보수교육 유형 중 82.6%가 기관개발교육이었다. 이수자의 면허취득경과연수에 따라 보수교육 프로그램, 보수교육 유형, 보수교육 내용의 참여에 유의한 차이를 보였으며 특히 면허취득경과연수가 길수록 근무지가 병원이 아닌 기타기관에 종사하는 대상자가 많았고 이수한 교육내용을 보면 지역보건, 직무역량이 증가하여 경력단계별 교육내용 요구도 파악 및 내용의 차별화가 필요함을 알 수 있다.

• Asian Pacific Journal of Cancer Prevention
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• 제16권6호
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• pp.2341-2345
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• 2015

This study aimed to investigate the relationship between prognosis and protein and mRNA expression of an apoptotic inhibitor gene, survivin, in patients with nasopharyngeal carcinoma. Furthermore, functions of the survivin gene in the CNE2 nasopharyngeal carcinoma cell line were assessed. Immunohistochemistry and in situ hybridization were used in detecting the survivin protein and mRNA in 44 nasopharyngeal carcinoma specimens, and 30 chronic nasopharyngitis samples as controls. Survivin gene expression in CNE2 cell line was suppressed with an shRNA (short hairpin RNA). The positive ratios of expression for survivin protein and mRNA in nasopharyngeal carcinoma were 79.5% and 75.0% respectively, obviously higher than in the control group (p<0.01), and there is very good consistency between the two methods. The mean survival time of patients with higher survivin protein or mRNA expression was shorter than in patients with lower levelsv(p<0.01). Proliferation of the CNE2 cell line was distinctly inhibited by the shRNA. The results indicate that overexpression of the survivin gene plays an important role in onset and development of nasopharyngeal carcinoma, and it may be helpful for prognostic appraisal.

• Asian Pacific Journal of Cancer Prevention
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• 제14권3호
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• pp.1787-1790
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• 2013

This study evaluated the effects of ZD1839, an orally active, selective epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor, on nasopharyngeal carcinoma (NPC) both in vitro and in vivo. Influence of ZD1839 alone or combined with cisplatin on the NPC cell line CNE2 was detected by MTT assay with flow cytometry assessment of cell cycle distribution and apoptosis rates. Nude mice NPC xenografts were also used to evaluate the effects of ZD1839 alone or combined with cisplatin. The Student's t test evaluated statistical significance. ZD1839 alone or combined with cisplatin inhibited CNE2 cell line proliferation. ZD1839 induced CNE2 cell cycle arrest in the G1 phase, and higher concentrations induced apoptosis. Xenograft tumors were significantly smaller when treated with 200 mg/kg ZD1839, cisplatin, or cisplatin combined with 100 mg/kg ZD1839 than untreated controls. ZD1839 (200 mg/kg) alone showed good tumor inhibition effects, reduction of tumor weights, and smaller tumor volume without loss of body weight. ZD1839 (200 mg/kg) might provide a good and effective therapeutic reagent for NPC.

• Archives of Pharmacal Research
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• 제29권12호
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• pp.1125-1131
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• 2006

This study was undertaken to elucidate the effect of diallyl disulfide (DADS), an oil-soluble organosulfur compound found in garlic, in suppressing human nasopharyngeal carcinoma cells. A potent increase (of at least 9-fold) in apoptotic cells has accompanied 1) a decrease in cell viability, 2) a increase of the fraction of S-phase cells by up to 63.8%, and 3) a transient increase of the phospho-p38 and phospho-p42/44 (phosphorylated p38 MAPK and phosphorylated p42/44 MAPK) in a time-and concentration-dependent manner. These results indicate that DADS can induce apoptosis in human nasopharyngeal carcinoma cells via, at least partly, S-phase block of the cell cycle, related to a rise in MAPK phosphorylation.

• 생약학회지
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• 제48권4호
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• pp.268-272
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• 2017

Three tricyclic pyridone alkaloids, (-)-4,6'-anhydrooxysporidinone (1), (-)-6-deoxyoxysporidinoCne (2), and (-)-sambutoxin (3) were isolated from cultures of Fusarium lateritium SSF2 derived from the fruits of Cornus officinalis. Chemical structures of the isolated compounds were determined by spectroscopic method and comparisons with reported data values. The compounds 1-3 were isolated for the first time from this fungal strain.