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http://dx.doi.org/10.7314/APJCP.2014.15.17.7065

Mangiferin Induces Apoptosis by Regulating Bcl-2 and Bax Expression in the CNE2 Nasopharyngeal Carcinoma Cell Line  

Pan, Li-Li (Department of Biochemistry and Molecular Biology, Guangxi Medical University)
Wang, Ai-Yan (Department of Biochemistry and Molecular Biology, Guangxi Medical University)
Huang, Yong-Qi (Department of Biochemistry and Molecular Biology, Guangxi Medical University)
Luo, Yu (Department of Biochemistry and Molecular Biology, Guangxi Medical University)
Ling, Min (Department of Biochemistry and Molecular Biology, Guangxi Medical University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.15, no.17, 2014 , pp. 7065-7068 More about this Journal
Abstract
To investigate the anti-proliferative mechanism of mangiferin in a human nasopharyngeal carcinoma cell line, CNE2 cells were incubated with different concentrations of mangiferin (12.5, 25, 50, 100, 150 and $200{\mu}M$) or with PBS as a control for 72 hours. Analyses were made of the cell cycle and apoptosis with measurement of mRNA and protein levels of two apoptosis-related genes, Bcl-2 and Bax. Flow cytometry assays showed mangiferin could inhibit CNE2 cell proliferation via G2/M arrest and induction of early apoptosis. Real time PCR and Western blotting showed the mRNA and protein level of Bcl-2 to be down-regulated, while those of Bax were upregulated, when CNE2 cells were treated with mangiferin. This investigation indicated anti-proliferation effects of mangiferin through induction of cell apoptosis regulated by Bcl-2 and Bax expression.
Keywords
Mangiferin; CNE2; apoptosis; Bcl-2; Bax;
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