• Journal of Microbiology and Biotechnology
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• 제27권4호
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• pp.791-807
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• 2017

The type II secretion system (T2SS), which transports selected periplasmic proteins across the outer membrane, has rarely been studied in nonpathogens or in organisms classified as Betaproteobacteria. Therefore, we studied Cupriavidus metallidurans (Cme), a facultative chemilithoautotroph. Gel analysis of extracellular proteins revealed no remarkable differences between the wild type and the T2SS mutants. However, enzyme assays revealed that native extracellular alkaline phosphatase is a T2SS substrate, because activity was 10-fold greater for the wild type than a T2SS mutant. In Cme engineered to produce three Ralstonia solanacearum (Rso) exoenzymes, at least 95% of their total activities were extracellular, but unexpectedly high percentages of these exoenzymes remained extracellular in T2SS mutants cultured in rich broth. These conditions appear to permit an alternative secretion process, because neither cell lysis nor periplasmic leakage was observed when Cme produced a Pectobacterium carotovorum exoenzyme, and wild-type Cme cultured in minimal medium secreted 98% of Rso polygalacturonase, but 92% of this exoenzyme remained intracellular in T2SS mutants. We concluded that Cme has a functional T2SS despite lacking any abundant native T2SS substrates. The efficient secretion of three foreign exoenzymes by Cme is remarkable, but so too is the indication of an alternative secretion process in rich culture conditions. When not transiting the T2SS, we suggest that Rso exoenzymes are probably selectively packaged into outer membrane vesicles. Phylogenetic analysis of T2SS proteins supports the existence of at least three T2SS subfamilies, and we propose that Cme, as a representative of the Betaproteobacteria, could become a new useful model system for studying T2SS substrate specificity.

• 한국산학기술학회논문지
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• 제16권3호
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• pp.2172-2177
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• 2015

상수 모듈러스 오차의 확률을 영으로 줄이는 등화 알고리듬에 결정 궤환 방식이 도입된 DF-MZEP-CME (decision feedback - maximum zero-error probability for constant modulus errors) 알고리듬은 채널 왜곡 보상에서 보다 향상된 성능을 보인다. 그러나 이 DF-MZEP-CME 알고리듬은 기울기 계산에서 샘플 사이즈에 비례하는 계산량을 가지게 되어 구현상 장애요인으로 작용한다. 이 논문에서는 DF-MZEP-CME 알고리듬의 기울기를 반복적으로 추정하도록 하여 계산량이 샘플 사이즈와 무관하게 함으로서 계산량 문제를 해결한다. 샘플 사이즈 N 에 대해 기존 알고리듬이 10N 의 곱셈량을 가지지만 제안한 방식은 샘플 사이즈와 무관하게 단지 20 번의 곱셈을 수행한다. 또한 제안한 방식의 기울기 계산이 초기상태로부터 안정 상태로 넘어갈 때 연속성을 유지하는 것으로 나타나 오차 전파에 예민한 결정 궤환 방식에 매우 적합한 알고리듬으로 판단된다.

• IMMUNE NETWORK
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• 제10권2호
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• pp.55-63
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• 2010

Background: Cordyceps militaris has been used in traditional medicine to treat numerous diseases and has been reported to possess both antitumor and immunomodulatory activities in vitro and in vivo. However, the pharmacological and biochemical mechanisms of Cordyceps militaris extract (CME) on macrophages have not been clearly elucidated. In the present study, we examined how CME induces the production of proinflammatory cytokines, transcription factor, and the expression of co-stimulatory molecules. Methods: We confirmed the mRNA and protein levels of proinflammatory cytokines through RT-PCR and western blot analysis, followed by a FACS analysis for surface molecules. Results: CME dose dependently increased the production of NO and proinflammatory cytokines such as IL-$1{\beta}$, IL-6, TNF-${\alpha}$, and $PGE_2$, and it induced the protein levels of iNOS, COX-2, and proinflammatory cytokines in a concentrationdependent manner, as determined by western blot and RT-PCR analysis, respectively. The expression of co-stimulatory molecules such as ICAM-1, B7-1, and B7-2 was also enhanced by CME. Furthermore, the activation of the nuclear transcription factor, NF-${\kappa}B$ in macrophages was stimulated by CME. Conclusion: Based on these observations, CME increased proinflammatory cytokines through the activation of NF-${\kappa}B$, further suggesting that CME may prove useful as an immune-enhancing agent in the treatment of immunological disease.

• 천문학회보
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• 제36권2호
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• pp.97.1-97.1
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• 2011

In this study we have examined the probability of solar proton events (SPEs) and their peak fluxes depending on flare (flux, longitude and impulsive time) and CME parameters (linear speed, longitude, and angular width). For this we used the NOAA SPE list and their associated flare data from 1976 to 2006 and CME data from 1997 to 2006. We find that about 3.5% (1.9% for M-class and 21.3% for X-class) of the flares are associated with SPEs. It is also found that this fraction strongly depends on longitude; for example, the fraction for $30W^{\circ}$ < L < $90W^{\circ}$ is about three times larger than that for $30^{\circ}E$ < L < $90^{\circ}E$. The SPE probability with long duration (${\geq}$ 0.3 hours) is about 2 (X-class flare) to 7 (M-class flare) times larger than that for flares with short duration (< 0.3 hours). In case of halo CMEs with V ${\geq}$ 1500km/s, 36.1% are associated with SPEs but in case of partial halo CME ($120^{\circ}$ ${\leq}$ AW < $360^{\circ}$) with 400 km/s ${\leq}$ V < 1000 km/s, only 0.9% are associated with SPEs. The relationships between X-ray flare peak flux and SPE peak flux are strongly dependent on longitude and impulsive time. The relationships between CME speed and SPE peak flux depend on longitude as well as direction parameter. From this study, we suggest a new SPE forecast method with three-steps: (1) SPE occurrence probability prediction according to the probability tables depending on flare and CME parameters, (2) SPE flux prediction from the relationship between SPE flux and flare (or CME) parameters, and (3) SPE peak time.

• 대한한의학회지
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• 제26권1호
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• pp.37-45
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• 2005

In the present study, we examined the effect of crude methanolic extract (CME) from Euonymus alatus (Thunb.) Sieb on arachidonic acid (AA) cascade in SKBR3 human breast cancer cell line. CME had a potent inhibitory activity of prostaglandin E2 (PGE2) release induced by A23187, a $Ca^{2+}$ ionophore. The inhibition was concentration-dependent, with the 50 value of about 5 M. CME had no inhibitory effect on A23187-induced phosphorylation of p42/p44 extracellular signal regulated kinase/mitogen-activated protein kinase or on the liberation of [14C]-AA from the cells labeled with [14C]-AA. However, CME concentration-dependently inhibited the conversion of AA to $PGE_2$ in microsomal preparations, showing its possible inhibition of cyclooxygenase (COX). In enzyme assay in vitro, CME inhibited the activities of both constitutive COX (COX­I) and inducible COX (COX-2) in a concentration-dependent manner, with the 50 values of about 0.8 and 2M, respectively. Lineweaver-Burk plot analysis indicated that CME competitively inhibited the activities of both COX-l and -2. This study is a first demonstration that CME directly inhibits COX activity.

• Asian-Australasian Journal of Animal Sciences
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• 제30권6호
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• pp.828-833
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• 2017

Objective: The study was conducted to evaluate the effects of different methionine (Met) sources on production performance, reproduction performance, egg quality and serum biochemical indices in broiler breeders. Methods: After receiving a basal diet (containing 0.25% Met) for a 2-wk pretreatment period, a total of 360 39-wk-old Lingnan yellow broiler breeders were randomly allocated to four treatments with six replicates each (15 birds per replicate). Breeders were fed with basal diets (control) or diets supplemented with DL-methionine (DLM), DL-2-hydroxy-4-methylthio butytric calcium (MHA-Ca) and coated DL-Met (CME) respectively. Results: The results showed that CME supplementation promoted laying rate and decreased feed-to-egg ratio (F/E) (p<0.05), DLM and MHA-Ca supplementation decreased F/E (p<0.05) when compared with control group. The rate of fertility, hatchability and birthrate were higher (p<0.05) in DLM, MHA-Ca, and CME groups than control group. Compared with control group, CME increased the eggshell thickness (p<0.05); MHA-Ca improved the eggshell thickness, shell ratio and eggshell strength (p<0.05). Results also showed that CME elevated the concentration of total protein in serum (p<0.05); MHA-Ca improved the concentration of calcium in serum (p<0.05). The concentration of serum uric acid in DLM, MHA-Ca, and CME groups was lower than that in control group (p<0.05). Besides, CME had higher concentrations of serum taurine, cysteine and cystanthionine (p<0.05) while MHA-Ca and DLM promoted the serum taurine concentration (p<0.05) compared with control group. Conclusion: Based on the results, it was concluded that Met supplementation could enhance the production and reproduction performance as well as the antioxidant status and egg quality of broiler breeders. In terms of improving the production performance, reproduction performance and antioxidant performance, CME was superior to DLM and MHA-Ca; but in regard to the enhancement of eggshell quality and serum Ca concentration, MHA-Ca was superior to DLM and CME.

• Journal of Astronomy and Space Sciences
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• 제25권4호
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• pp.383-394
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• 2008

코로나질량방출(CMEs)의 지구영향도를 효율적으로 예측하기 위해 속도가 빠른 CMEs의 방향지시 매개인수(parameter)를 조사하여 Dst 지수 최소값과의 상관관계를 비교하였다. 그리고 이 매개인수 중 어떤 매개인수가 예측 효율성이 높은지 알아보았다. 이를 위해 SOHO/LASCO 목록에서 CMEs의 속도가 1000km/s 이상, CMEs발생 위치가 태양 중심에서 ${\pm}30^{\circ}$ 이내에 위치한 혜일로(halo) CMEs를 사용하였다. 이 CMEs가 태양에서 발생하여 지구에 도착하는 시각을 모델을 이용하여 예측한 뒤 이 시각에 가장 가까운(24시간 이내) Dst 지수 최소값을 구하였다. 이를 통해 CMEs의 지구 영향도를 판별하였다. 전체 30개의 사건 중 22개(73%)가 지자기 폭풍을 일으켰다. 우리는 CMEs의 지구 영향도를 예측하기 위해 기존의 방향 매개인수와 이번 연구에서 새롭게 제안한 이심을, 이동거리, 중심각 매개인수를 사용하였다. 이들 매개인수와 Dst 지수 최소값의 상관관계 분석을 통해 상관계수 값을 알아보고 그 결과를 통해 어떤 매개인수가 CMEs의 지구 영향도 예측 효율이 높은지 비교하였다. 그 결과 이심율 매개인수가 가장 좋은 상관관계를 보였고, 방향 매개인수는 비교적 좋은 상관관계를 보였다. 이동거리와 중심각 매개인수는 이심율과 방향 매개인수보다 낮은 상관관계를 보였다. 하지만 이들 역시 강한 지자기 폭풍(Dst 지수 ${\leq}$-200nT)의 경우에는 좋은 상관관계를 보였다. 방향 매개인수 값이 0.6 이상이고 이심율 매개인수 값이 0.4 이하이며 이동거리, 중심각 매개인수 값이 0.2 이하인 경우에는 강한 지자기 폭풍(Dst 지수 ${\leq}$-200nT)이 일어날 확률이 아주 높음을 알 수 있었다. CMEs의 방향을 지시하는 매개인수는 CMEs의 지구 영향도를 판별하는데 유용하고 이를 복합적으로 고려한다면 예측의 효율성을 높일 수 있다.

• The Korean Journal of Physiology and Pharmacology
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• 제27권2호
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• pp.143-155
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• 2023

Percutaneous coronary intervention and acute coronary syndrome are both closely tied to the frequently occurring complication of coronary microembolization (CME). Resveratrol (RES) has been shown to have a substantial cardioprotective influence in a variety of cardiac diseases, though its function and potential mechanistic involvement in CME are still unclear. The forty Sprague-Dawley rats were divided into four groups randomly: CME, CME + RES (25 mg/kg), CME + RES (50 mg/kg), and sham (10 rats per group). The CME model was developed. Echocardiography, levels of myocardial injury markers in the serum, and histopathology of the myocardium were used to assess the function of the cardiac muscle. For the detection of the signaling of TLR4/MyD88/NF-κB along with the expression of pyroptosis-related molecules, ELISA, qRT-PCR, immunofluorescence, and Western blotting were used, among other techniques. The findings revealed that myocardial injury and pyroptosis occurred in the myocardium following CME, with a decreased function of cardiac, increased levels of serum myocardial injury markers, increased area of microinfarct, as well as a rise in the expression levels of pyroptosis-related molecules. In addition to this, pretreatment with resveratrol reduced the severity of myocardial injury after CME by improving cardiac dysfunction, decreasing serum myocardial injury markers, decreasing microinfarct area, and decreasing cardiomyocyte pyroptosis, primarily by blocking the signaling of TLR4/MyD88/NF-κB and also reducing the NLRP3 inflammasome activation. Resveratrol may be able to alleviate CME-induced myocardial pyroptosis and cardiac dysfunction by impeding the activation of NLRP3 inflammasome and the signaling pathway of TLR4/MyD88/NF-κB.

• 천문학회보
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• 제37권1호
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• pp.92.1-92.1
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• 2012

In this work we have examined the performance of the WSA/ENLIL cone model provided by Community Coordinated Modeling Center (CCMC). The WSA/ENLIL model simulates the propagation of coronal mass ejections (CMEs) from the Sun into the heliosphere. We estimate the shock arrival times at the Earth using 29 halo CMEs from 2001 to 2002. These halo CMEs have cone model parameters from Michalek et al. (2007) as well as their associated interplanetary (IP) shocks. We make a comparison between CME arrival times by the WSA/ENLIL cone model and IP shock observations. For the WSA/ENLIL cone model, the root mean square(RMS) error is about 13 hours and the mean absolute error(MAE) is approximately 10.4 hours. We compared these estimates with those of the empirical model by Kim et al.(2007). For the empirical model, the RMS and MAE errors are about 10.2 hours and 8.7 hours, respectively. We are investigating several possibilities on relatively large errors of the WSA/ENLIL cone model, which may be caused by cone model velocities, CME density enhancement factor, or CME-CME interaction.

• 천문학회지
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• 제53권1호
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• pp.1-7
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• 2020

The recent study of Chae et al. (2017) found a one-to-one correspondence between plasma blobs outflowing along a ray formed after a coronal mass ejection (CME) and small X-ray flares. In the present work, we have examined the spatial configuration and the eruption process of the flares that are associated with the blobs by analyzing EUV images and magnetograms taken by the SDO/AIA and HMI. We found that the main flare and the successive small flares took place in a quadrupolar magnetic configuration characterized by predominant magnetic fields of positive polarity, two minor magnetic fragments of negative polarity, and a curved polarity inversion line between them, which suggests that the formation process of the blobs may be similar to that of the parent CME. We also found that the successive flares resulted in a gradual change of the quadrupolar magnetic configuration, and the relevant migration of flaring kernels. The three-dimensional geometry and the property of the current sheet, that is often supposed to be embedded in an observed post-CME ray, seem to keep changing because of mutual feedback between the successive flares and the temporal change of the magnetic field configuration. Our results suggest that the observed post-CME rays may not reflect the characteristics of the current sheet responsible for the impulsive phase of the flare.