• International Journal of Oral Biology
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• v.32 no.3
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• pp.109-112
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• 2007

Somatic cells nuclear transfer (SCNT) is a useful tool in studies of developmental biology and animal cloning. However, SCNT experiments only are allowed to skilled technical experts. In this experiment, laser-assisted zona pellucida piercing tool (LASER) was applied in murine SCNT. LASER minimized the use of piezo-driven micromanipulator (PIEZO), reducing chances of problems caused by PIEZO pulses. LASER reduced time that took to pierce zona pellucida in removal of nucleus from oocyte and somatic cell injection, which might have taken longer time with PIEZO. Time and difficulties that took researcher of equivalent skilled for their experiments were decreased with LASER, and this might affect the improvement of embryonic development. (LASER, 6.2% versus PIEZO, 2.9%; P<0.05). Thus, these data support that the use of LASER can be used for zona pellucida piercing in murine SCNT program as an alternative to PIEZO.

• Molecules and Cells
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• v.40 no.4
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• pp.307-313
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• 2017

Caloric restriction (CR) has been shown to extend lifespan and prevent cellular senescence in various species ranging from yeast to humans. Many effects of CR may contribute to extend lifespan. Specifically, CR prevents oxidative damage from reactive oxygen species (ROS) by enhancing mitochondrial function. In this study, we characterized 33 single electron transport chain (ETC) gene-deletion strains to identify CR-induced chronological lifespan (CLS) extension mechanisms. Interestingly, defects in 17 of these 33 ETC gene-deleted strains showed loss of both respiratory function and CR-induced CLS extension. On the contrary, the other 16 respiration-capable mutants showed increased CLS upon CR along with increased mitochondrial membrane potential (MMP) and intracellular adenosine triphosphate (ATP) levels, with decreased mitochondrial superoxide generation. We measured the same parameters in the 17 non-respiratory mutants upon CR. CR simultaneously increased MMP and mitochondrial superoxide generation without altering intracellular ATP levels. In conclusion, respiration is essential for CLS extension by CR and is important for balancing MMP, ROS, and ATP levels.

• Proceedings of the KIEE Conference
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• 2006.07d
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• pp.1846-1847
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• 2006

In this study, we developed the real-time controller for control loading system (CLS) of aircraft simulator. The CLS is given the forces as inputs: the exerted force by a pilot, which is determined according to the position of the control stick, and the calculated force by the host computer. And then CLS makes the pilot feel the back loading force by supplying the motor drive with the actuator signal. The developed real-time controller for CLS is organized into the five parts which are the position sensing part including a encoder, the A/D converter part for the analog load cell signal, the communication interface part to communicate with the host, the D/A converter for the actuator signal, and the CPU DSP2812 to carry out a control algorithm. We constructed the test control loading system and carried out the experiment with the developed real-time controller. The experimental results showed that the real-time controller generates the back loading forces similar to the desired back loading force graph.

• Structural Engineering and Mechanics
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• v.64 no.4
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• pp.487-494
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• 2017

The present paper deals with a theoretical study of delamination fracture in the Crack Lap Shear (CLS) functionally graded beam configuration. The basic purpose is to analyze the fracture with taking into account the material non-linearity. The mechanical behavior of CLS was described by using a non-linear stress-strain relation. It was assumed that the material is functionally graded along the beam height. The fracture was analyzed by applying the J-integral approach. The curvature and neutral axis coordinate of CLS beam were derived in order to solve analytically the J-integral. The non-linear solution of J-integral obtained was verified by analyzing the strain energy release rate with considering material non-linearity. The effects of material gradient, crack location along the beam height and material non-linearity on fracture behavior were evaluated. The J-integral non-linear solution derived is very suitable for parametric studies of longitudinal fracture in the CLS beam. The results obtained can be used to optimize the functionally graded beam structure with respect to the fracture performance. The analytical approach developed in the present paper contributes for the understanding of delamination fracture in functionally graded beams exhibiting material non-linearity.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.12 no.8
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• pp.3516-3541
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• 2018

Task scheduling is one of the most challenging aspects of cloud computing nowadays, and it plays an important role in improving overall performance in, and services from, the cloud, such as response time, cost, makespan, and throughput. A recent cloud task-scheduling algorithm based on the symbiotic organisms search (SOS) algorithm not only has fewer specific parameters, but also incurs time complexity. SOS is a newly developed metaheuristic optimization technique for solving numerical optimization problems. In this paper, the basic SOS algorithm is reduced, and chaotic local search (CLS) is integrated into the reduced SOS to improve the convergence rate. Simulated annealing (SA) is also added to help the SOS algorithm avoid being trapped in a local minimum. The performance of the proposed SA-CLS-SOS algorithm is evaluated by extensive simulation using the Matlab framework, and is compared with SOS, SA-SOS, and CLS-SOS algorithms. Simulation results show that the improved hybrid SOS performs better than SOS, SA-SOS, and CLS-SOS in terms of convergence speed and makespan.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.23 no.7
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• pp.1840-1850
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• 1998

In this paper, we propose a priority control algorithm using hop count, called HC-PC(Priority Control Algorithm using Hop-Count) for steaming mode CLS, in wide area ATM networks. In our HC-PC algorithm, hop count of packet is increased by one as traverse CLS hop and is used as loss priority when a CLS buffer is congested. That guards packets with higher priority form congestion. The average packet loss probabilities are evaluated via mathematical analysis for HC-PC algorithm case and no priority control case. The simulationresults indicate that our algorithm may offer better performance in terms of loss fairness for pathe length and yield high utilization of newtork resource.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.17 no.1
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• pp.3-10
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• 2007

In this paper, we show that the status certificate-based encryption(SCBE) scheme proposed at ICISC 2004 and the certificateless signature(CLS) scheme proposed at EUC workshops 2006 are insecure. Both schemes are claimed that an adversary has no advantage if it controls only one of two participants making a cryptographic key such as a decryption key in SCBE or a signing key in CLS. But we will show that an adversary considered in the security model of each scheme can generate a valid cryptographic key by replacing the public key of a user.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.32 no.4
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• pp.66-73
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• 2000

Alkaline sulfite-anthraquinone(AS-AQ) cooking process has been applied to unground pine bark. The properties of the spent liquor such as molecular weight distributions, sulfonic equivalent weights, degree of sulfonation, phenolic hydroxyl groups and kaolin dispersing ability have been investigated to evaluate the possibility of using this liquor as concrete additives or binders. In the case of ground bark meal, more than 90% delignification was achieved at the optimal cooking conditions. However, applying these conditions on unground bark the delignification was slightly retarded. The decrease in the degree of delignification may be attributed to less penetration and diffusion of chemicals during the cooking of the bark. Increasing the cooking temperature only by $5^{\circ}C$ improved the delignification of the bark and about 90% delignification can be achieved. These results indicate that bark can be used successfully during AS-AQ cooking without any mechanical or physical pretreatment. The properties of lignin or polyphenol sulfonates in the spent liquor after AS-AQ cooking of the bark were compared with Sunflo-R, which is commercial lignosulfonate(CLS) prepared from wood. The weight average molecular weights(Mws) estimated by gel-filteration chromatography was found to be ranging from 1,200~1,800. These are considerably lower than those in CLS. Lignin or polyphenol sulfonates in the spent liquor of bark and CLS have similar degree of sulfonation, but the phenolic hydroxyl group was 1.8 times higher than CLS. Moreover, the dispersing abilities of the spent liquors were better than that of CLS, especially after 0.1% addition to kaolin suspension.

• Korean Journal of Health Psychology
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• v.19 no.1
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• pp.407-420
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• 2014

In this study, a Korean abbreviation scale to measure love and compassion toward other person was developed based on a critical review on Compassionate Love Scale(CLS; Sprecher, & Fehr, 2005). In the study 1, 12 items were selected from the original version of 21 item CLS by surveying a sample of 207 college students on the basis of several psychometric characteristics (i.e., discriminability, coefficient of factor loading, and contribution to internal consistency). In the study 2, the validity of 12 item version, ACLS (Ajou Compassionate Love Scale), was confirmed by examining the factor structure, reliability, and correlations with other related scales. The results clearly showed that ACLS not only had a sufficient psychometric properties as the CLS, but also superior to the CLS in terms of parsimoniousness. Limitations and implications for future research of ACLS were discussed.

• Journal of Interdisciplinary Genomics
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• v.2 no.1
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• pp.10-12
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• 2020

Coffin-Lowry syndrome (CLS) is a genetic disorder characterized by intellectual disability, typical facial features, and skeletal abnormalities. But this syndrome shows highly variable clinical manifestations, and can't be diagnosed with conventional chromosome analysis or comparative genomic hybridization, leading to delayed diagnosis. Here we report an 18-year-old boy with CLS diagnosed by whole exome sequencing. Our patient initially presented with developmental delay, facial dysmorphism at the age of 1. At the age of 18, he developed orthopnea due to mitral regurgitation. At the 22 years of age, he was diagnosed as CLS diagnosed by whole exome sequencing. Our case implies that clinical suspicion is important for early diagnosis, and advanced diagnostic tools such as WES should be considered in suspected cases.