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A Comparative Study on the Chemical Characteristics and Antioxidant Effects of Sea Mustards Sourced from Different Areas in Taejongdae (태종대산 5종 돌미역의 화학성분 및 항산화활성 비교)

  • Kim, Hojun;Jayapala, HPS;Jo, Won Hee;Nam, Hyung Sik;Lim, Sun Young
    • Journal of Life Science
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    • v.31 no.6
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    • pp.559-567
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    • 2021
  • This study compared the nutritional characteristics and antioxidant effects of sea mustards sourced from five different areas (Barammaegi, Gultongmeori, Chanmulgae, Johongtaek, and Goraedeung) in Taejongdae, Youngdo, Busan. The contents of total flavonoids and phenols and fatty acid composition were measured. To evaluate their antioxidant effects, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays were used. Acetone/methylene chloride (A+M) extracts from all the sea mustards contained higher amounts of total flavonoids and phenols than methanol (MeOH) extracts. Among the sea mustards obtained from the different areas, the total flavonoid and total phenolic content of the A+M extract of the sea mustard from Gultongmeori was 1.44±0.04 mg/g and 1.72±0.06 mg/g, respectively. In terms of the fatty acid composition, the Gultongmeori sea mustard had higher percentages of total n-6, total n-3, eicosapentaenoic acid (EPA, 20:5n-3), and docosahexaenoic acid (DHA, 22:6n-3) than the sea mustards from the other areas. The A+M extract of the sea mustard from Gultongmeori was more effective in terms of scavenging free radicals as compared with that of the other sea mustards, as assessed by the DPPH and ABTS assays (p<0.05). In a 120-minute reactive oxygen species (ROS) production assay, all the extracts tested decreased cellular ROS production induced by H2O2 compared to that produced by exposure to an extract-free control (p<0.05). The extracts from Barammaegi and Gultongmeori had a greater inhibitory effect on cellular ROS production. These results indicated that the antioxidant effects of sea mustards might be associated with a higher amount of flavonoids and phenols. This study suggests that food-processed products from sea mustard can be developed as functional foods for promoting health in the local population.

Survival and Growth Characteristics of Foodborne Pathogen in Romaine Lettuce (로메인 상추에서 병원성미생물의 생존 및 증식 특성)

  • Kim, Na-Ye Seul;Kim, Chae Rin;Kim, Da-Woon;Jeong, Myung-In;Oh, Kwang Kyo;Kim, Bo-Eun;Ryu, Jae Gee;Jung, Jieun;Jeon, Ik Sung;Ryu, Kyoung-Yul
    • Journal of Food Hygiene and Safety
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    • v.36 no.6
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    • pp.481-487
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    • 2021
  • The purpose of this study was to provide safety management information by analyzing the survival and growth-related properties of foodborne pathogens from Romaine lettuce. After cultivating E. coli O157:H7 for 72 h on Romain lettuce via spray inoculation, the bacteria population increased by 2.0 log CFU/g from the initial population, confirming the possibility of survival and multiplication of the pathogen thereon. The study also revealed that there is no significant difference in the cultivation of E.coli O157:H7 after 72 h from inoculation on damaged and undamaged lettuce leaves. As a result of investigating distribution of E.coli O157:H7 on damaged lettuce leaves, it was found that the bacteria is unlikely to adhere on the smooth surface of undamaged leaves and, thus, results in a low population density, whereas the bacteria cluster on the rough surface of damaged leaves and easily enter through the damaged tissues. Furthermore, after 24 h of cultivation of the pathogenic microbe in the extract with concentrations of 10-100%, utilization of the lettuce extract by the pathogen was found to be 8.9 log CFU/mL E. coli O157:H7, 8.6 log CFU/mL L. monocytogenes, and 8.8 log CFU/mL P. carotovorum. The increase in the population of both the pathogenic microbe and foodborne pathogen reached over 4 log CFU/mL, implying the microbe can utilize the lettuce extract as a source of nutrition. Compared to the initial inoculation concentration in 0.1% lettuce extract, the final concentration has increased up to 2.7 log CFU/mL E. coli O157:H7, 1.3 log CFU/mL L. monocytogenes, and 2.9 log CFU/mL P. carotovorum. Accordingly, the study confirms that the minimal growth concentration of the pathogenic microbe is lower than 0.1% and that the pathogen possibly survive and multiply inside the lettuce leaves given the lettuce extract with concentration of 0.1% is consistently supplied through the damaged tissues.

Spatial Distribution of Macrozoobenthic Organisms along the Korean Coasts in Summer Season (한국 연안의 하계 대형저서동물의 공간분포)

  • LEE, JUNG-HO;LIM, HYUN-SIG;SHIN, HYUN CHOOL;RYU, JONGSEONG
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.27 no.2
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    • pp.87-102
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    • 2022
  • To clarify the spatial distribution pattern of macrozoobenthos in Korean coastal waters in the summer season and investigate the relationship between community structure and benthic environmental factors, field surveys on community structure and benthic environmental factors were conducted at 117 stations in August 2017. A total of 613 macrobenthic species were identified, with the mean density of 1,228 ind./m2 and the mean biomass (wet weight) of 110.9 g WW/m2. Rich biodiversity was found at stations near Wando and along the coast of the East Sea, and there is a trend that stations with greater biodiversity also showed higher mean density as well. The dominant species in all the coastal areas in Korea was Heteromastus filiformis, which were found at most of the stations during the survey. The relatively deep areas in the East Sea were dominated by Magelona johnsoni and Maldane cristata, which were the third and ninth dominant species in the study areas, respectively. Pseudopolydora kempi and Rhynchospio sp. were observed only at the station located in the Nakdong River estuary. From the cluster analysis the stations could be clustered into three station groups with more similar faunal composition. Group A was located in the eastern coast, characterized with deep water depth and low levels of sand contents, while Group B was located in the southern coast, characterized by shallow depth of water and high content of mud and organic matter. Lastly, Group C was in the western coast, demonstrating low levels of mud content and organic matters. The biodiversity of macrobenthic species in the study area showed high positive correlation coefficients with benthic environmental factors such as sorting, clay, silt, and contents of organic matter in sediments, but negatively correlated with the sand contents. Major dominant species, Theora lata and Eriopisella sechellensis, both showed negative correlation coefficients with the sand contents, but a relatively high positive correlation with the levels of organic contents.It can be concluded that the spatial distribution patterns of macrobenthic organisms in Korean coastal waters are affected by depth, sediment type, and contents of organic matters.

Grapevine Growth and Berry Development under the Agrivoltaic Solar Panels in the Vineyards (영농형 태양광 시설 설치에 따른 포도나무 생육 및 과실 특성 변화 비교)

  • Ahn, Soon Young;Lee, Dan Bi;Lee, Hae In;Myint, Zar Le;Min, Sang Yoon;Kim, Bo Myung;Oh, Wook;Jung, Jae Hak;Yun, Hae Keun
    • Journal of Bio-Environment Control
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    • v.31 no.4
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    • pp.356-365
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    • 2022
  • Agrivoltaic systems, also called solar sharing, stated from an idea that utilizes sunlight above the light saturation point of crops for power generation using solar panels. The agrivoltaic systems are expected to reduce the incident solar radiation, the consequent surface cooling effect, and evapotranspiration, and bring additional income to farms through solar power generation by combining crops with solar photovoltaics. In this study, to evaluate if agrivoltaic systems are suitable for viticulture, we investigated the microclimatic change, the growth of vines and the characteristics of grape grown under solar panels set by planting lines compared with ones in open vineyards. There was high reduction of wind speed during over-wintering season, and low soil temperature under solar panel compared to those in the open field. There was not significant difference in total carbohydrates and bud burst in bearing mother branches between plots. Despite high content of chlorophyll in vines grown under panels, there is no significant difference in shoot growth of vines, berry weight, cluster weight, total soluble solid content and acidity of berries, and anthocyanin content of berry skins in harvested grapes in vineyards under panels and open vineyards. It was observed that harvesting season was delayed by 7-10 days due to late skin coloration in grapes grown in vineyards under panels compared to ones grown in open vineyards. The results from this study would be used as data required in development of viticulture system under panel in the future and further study for evaluating the influence of agrivoltaic system on production of crops including grapes.

Revealing the Paleo-ocean Environment of OSM-XX in the Western Pacific Magellan Seamount with Mineralogical and Geochemical Properties of Ferromanganese Crust (서태평양 마젤란해산군 OSM-XX 해저산 망간각의 광물학적, 지화학적 특성과 고해양 고환경 복원 연구)

  • Jinsub Park;Kiho Yang
    • Economic and Environmental Geology
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    • v.56 no.1
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    • pp.55-63
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    • 2023
  • Variations in geochemical and mineralogical properties of the ferromanganese(Fe-Mn) crust reflect environmental changes. In the present study, geochemical and mineralogical analyses, including micro X-ray fluorescence and X-ray diffraction, were utilized to reconstruct the paleo-ocean environment of western Pacific Magellan seamount cluster. Samples of the Fe-Mn crust were collected using an epibenthic sledge from the open seamount XX (151° 51.12' 7.2" E and 16° 8.16' 9.6" N, 1557 meters below sea level) in the Western Pacific Magellan Seamount. According to the structure and phosphating status, the Fe-Mn crust of the OSM-XX can be divided into the following: phosphatizated (L4-L5), massive non-phosphatizated (L3), and porous non-phosphatizated (L1-L2) portions. All ferromanganese layers contain vernadite, and owing to the presence of carbonate fluorapatite (CFA), the phosphatizated portion (L4-L5) is rich in Ca and P. The massive non-phosphatizated section (L3) contains high Mn, Ni, and Co, whereas the porous non-phosphatizated portion (L1-L2), which comprises detrital quartz and feldspar, is rich in Fe. Variations in properties of the Fe-Mn crust from the OSM-XX reflect changes in the nearby marine environment. The formation of this crust started at approximately 51.87 Ma, and precipitation of the CFA during the global phosphatization event that occurred at approximately 36-32 Ma highlights an elevated sea level and low temperature during the associated period. The high Mn, Ni, and Co concentrations and elevated Mn/Fe ratios of samples from the massive phosphatizated portion indicate that the oxygen minimum zone (OMZ) was enhanced, and reducing conditions prevailed during the crust formation. The high Fe and low Mn/Fe ratios in the porous portion indicate a weak OMZ and dominantly oxidizing conditions. These data reflect environmental changes following the end of the Mi-1 glacial period in the Miocene-Oligocene boundary. Subsequently, Mn/Fe and Co/Mn ratios increased slightly in the outermost part of Fe-Mn crust because of the enhanced bottom current and OMZ associated with the continued cooling from approximately 9 Ma. However, the reduced carbonate dissolution rate in the Pacific Ocean from approximately 6 Ma decreased the growth rate of the Fe-Mn crust.

Macrobenthic Community Structure during Spring and Summer Season in the Environmental Conservation Area, Korea (환경보전해역에 서식하는 대형저서동물의 춘계와 하계의 군집구조)

  • Choi, Byoung-Mi;Yun, Jae Seong;Kim, Seong Gil;Kim, Seong-Soo;Choi, Ok In;Son, Min Ho;Seo, In-Soo
    • Journal of Marine Life Science
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    • v.1 no.2
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    • pp.95-108
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    • 2016
  • This study was performed to investigate the community structure of macrobenthic assemblages in the Environmental Conservation area, Korea. Benthic animals were collected by van Veen grab sampler at spring (May) and summer (August) 2009. The total species number and mean density were 195 species 5.6 m-2 and 667 individuals m-2, respectively. Polychaetes were the most dominant faunal group in species (96 species) and abundance (431 individuals m-2). The major dominant species were the polychaetes Lumbrineris longifolia (76±224 individuals m-2), Mediomastus californiensis (42±117 individuals m-2), Tharyx sp.3 (26±110 individuals m-2), the bivalvia Theora fragilis (54±78 individuals m-2) and the amphipod Eriopisella schellensis (70±146 individuals m-2). Based on the cluster and nMDS ordination analysis, macrobenthic communities were divided into three faunal groups. The first group was characterized by high abundance of the polychaeta Sternaspis scutata and the amphipod Ampelisca cyclops iyoensis, which is located by most stations of Hampyeong Bay and St. 4 of Deungnyang Bay. The second group was numerically dominated by the polychaeta Capitella capitata at St. 4 and St. 5 in Gamak Bay where was most pollutant area. Finally, the third group was dominated by the polychaetes Heteromastus filiformis, Tharyx sp.3 and the amphipod Sinocorophium sinensis. Therefore, geochemical characteristics such as the bay shape and pollution gradient may be important factors controlling of the macrobenthic community structure in Environment Conservation Area.

Fish Community Characteristics and Distribution Aspect of Rhodeus pseudosericeus(Cyprinidae) in the Geumdangcheon(Stream), a Tributary of the Hangang Drainage System of Korea (한강 지류 금당천의 어류군집 특징과 멸종위기종 한강납줄개의 서식양상)

  • Mee-Sook Han;Myeong-Hun Ko
    • Korean Journal of Environment and Ecology
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    • v.37 no.2
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    • pp.151-162
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    • 2023
  • This study investigated the characteristics of fish communities and inhabiting status of the endangered species, Rhodeus pseudosericeus, in the Geumdang Stream in Korea from March to October 2021. A total of 1,698 fish in 5 families and 25 species were collected from 7 survey stations during the survey period. The dominant species was Zacco platypus (relative abundance, 46.5%), and the subdominant species was Squalidus gracilis majimae (16.7%), followed by Rhynchocypris oxycephalus (12.0%), Z. koreanus (5.7%), Pungtungia herzi (3.2%), R. pseudosericeus (2.0%), R. notatus (1.9%), and Acheilognathus rhombeus (1.8%). Nine Korean endemic species (36.0%) were collected, including R. pseudosericeus, R. uyekii, Sarcocheilichthys variegatus wakiyae, Microphysogobio yaluensis, S. gracilis majimae, Z. koreanus, Cobitis nalbanti, Iksookimia koreensis, and Odontobutis interrupta. An exotic species, Micropterus salmoides, designated as an invasive alien species (IAS), was collected downstream. The investigation of the habitat patterns of the endangered species (class II), Rhodeus pseudosericeus, showed a habitat range of about 6 to 7 km in the middle of Geumdang Stream (RP-1 to RP-4), and this species inhabited the edge with water depths of 0.3 through 1.0 m with slow water flow and many aquatic plants. According to the community analysis results, the overall dominance and evenness indexes were low, while diversity and richness indexes were high, and the cluster structure was largely divided into upstream and middle-downstream areas. The river health (fish assessment index) evaluated using fish was assessed as good (3 stations), normal (3 stations), and bad (1 station), and water quality was evaluated as good both upstream and downstream. Compared to previous studies, the number of species was relatively similar, and among the species that appeared in the past, 13 species did not appear in this survey, while 6 species appeared for the first time in this survey. Disturbance factors included river construction, many weirs, and the appearance of the ecosystem-disturbing species, M. salmoides. Since Geumdang Strem has high conservation value because it is home to many species in the Acheilognathinae subfamily, including the endangered species R. pseudosericeus, continuous attention and systematic conservation measures are required.

Fish Community Characteristics and Distribution Aspect of Four Endangered Species in the Byekgye Stream, Korea (벽계천의 어류군집 특성 및 멸종위기 4종의 서식양상)

  • HyeongSu Kim;Myeong-Hun Ko
    • Korean Journal of Environment and Ecology
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    • v.38 no.1
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    • pp.55-66
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    • 2024
  • This study conducted a survey to investigate the characteristics of fish communities and the inhabiting status of endangered species in the Byekgye Stream, Korea from April to September 2020. A total of 3,415 fish of 9 families and 31 species were collected from 7 survey stations during the survey period. The dominant species was Zacco koreanus (relative abundance of 31.2%), and the subdominant species was Z. platypus (15.0%), followed by Pungtungia herzi (11.7%), Acheilognathus yamatsutae (5.4%), A. lanceolata intermedia (4.8%), Rhinogobius brunneus (4.4%), and Pseudopungtungia tenuicorpa (4.3%). Among the fish species collected, 19 (61.3%) were identified as Korean endemic species, and two cold-water fish species sensitive to climate change (Rhynchocypris kumgangensis and Cottus koreanus) were collected. Four species were designated as class II endangered wildlife by the Ministry of Environment: A. signifer, P. tenuicorpa, Rhodeus pseudosericeus, and C. koreanus. A. signifer and P. tenuicorpa mainly inhabited the mid to lower streams, R. pseudosericeus in the midstream, and R. pseudosericeus in the upstream. P. tenuicorpa inhabited in large numbers, and estimating the age by total length-frequency distribution in July, the total length of the 26-35 mm group was estimated as 0 years old, the 54-75 mm group as 1 year old, 82-97 mm group as 2 years old, 104-109 mm group as 3 years or older. The cluster analysis showed that the dominance index decreased from upstream to downstream, but the diversity, evenness, and richness index increased. The water quality of Byekgye Stream was evaluated as good overall since the river health (fish assessment index, FAI) using fish was evaluated as excellent (5 stations) and good (2 stations). Byekgye Stream has relatively well-preserved habitats, but conservation measures are required as habitats are disturbed by river repair work in some parts of the midstream and downstream areas where many endangered species inhabit.

Prediction of multipurpose dam inflow utilizing catchment attributes with LSTM and transformer models (유역정보 기반 Transformer및 LSTM을 활용한 다목적댐 일 단위 유입량 예측)

  • Kim, Hyung Ju;Song, Young Hoon;Chung, Eun Sung
    • Journal of Korea Water Resources Association
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    • v.57 no.7
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    • pp.437-449
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    • 2024
  • Rainfall-runoff prediction studies using deep learning while considering catchment attributes have been gaining attention. In this study, we selected two models: the Transformer model, which is suitable for large-scale data training through the self-attention mechanism, and the LSTM-based multi-state-vector sequence-to-sequence (LSTM-MSV-S2S) model with an encoder-decoder structure. These models were constructed to incorporate catchment attributes and predict the inflow of 10 multi-purpose dam watersheds in South Korea. The experimental design consisted of three training methods: Single-basin Training (ST), Pretraining (PT), and Pretraining-Finetuning (PT-FT). The input data for the models included 10 selected watershed attributes along with meteorological data. The inflow prediction performance was compared based on the training methods. The results showed that the Transformer model outperformed the LSTM-MSV-S2S model when using the PT and PT-FT methods, with the PT-FT method yielding the highest performance. The LSTM-MSV-S2S model showed better performance than the Transformer when using the ST method; however, it showed lower performance when using the PT and PT-FT methods. Additionally, the embedding layer activation vectors and raw catchment attributes were used to cluster watersheds and analyze whether the models learned the similarities between them. The Transformer model demonstrated improved performance among watersheds with similar activation vectors, proving that utilizing information from other pre-trained watersheds enhances the prediction performance. This study compared the suitable models and training methods for each multi-purpose dam and highlighted the necessity of constructing deep learning models using PT and PT-FT methods for domestic watersheds. Furthermore, the results confirmed that the Transformer model outperforms the LSTM-MSV-S2S model when applying PT and PT-FT methods.

An Expert System for the Estimation of the Growth Curve Parameters of New Markets (신규시장 성장모형의 모수 추정을 위한 전문가 시스템)

  • Lee, Dongwon;Jung, Yeojin;Jung, Jaekwon;Park, Dohyung
    • Journal of Intelligence and Information Systems
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    • v.21 no.4
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    • pp.17-35
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    • 2015
  • Demand forecasting is the activity of estimating the quantity of a product or service that consumers will purchase for a certain period of time. Developing precise forecasting models are considered important since corporates can make strategic decisions on new markets based on future demand estimated by the models. Many studies have developed market growth curve models, such as Bass, Logistic, Gompertz models, which estimate future demand when a market is in its early stage. Among the models, Bass model, which explains the demand from two types of adopters, innovators and imitators, has been widely used in forecasting. Such models require sufficient demand observations to ensure qualified results. In the beginning of a new market, however, observations are not sufficient for the models to precisely estimate the market's future demand. For this reason, as an alternative, demands guessed from those of most adjacent markets are often used as references in such cases. Reference markets can be those whose products are developed with the same categorical technologies. A market's demand may be expected to have the similar pattern with that of a reference market in case the adoption pattern of a product in the market is determined mainly by the technology related to the product. However, such processes may not always ensure pleasing results because the similarity between markets depends on intuition and/or experience. There are two major drawbacks that human experts cannot effectively handle in this approach. One is the abundance of candidate reference markets to consider, and the other is the difficulty in calculating the similarity between markets. First, there can be too many markets to consider in selecting reference markets. Mostly, markets in the same category in an industrial hierarchy can be reference markets because they are usually based on the similar technologies. However, markets can be classified into different categories even if they are based on the same generic technologies. Therefore, markets in other categories also need to be considered as potential candidates. Next, even domain experts cannot consistently calculate the similarity between markets with their own qualitative standards. The inconsistency implies missing adjacent reference markets, which may lead to the imprecise estimation of future demand. Even though there are no missing reference markets, the new market's parameters can be hardly estimated from the reference markets without quantitative standards. For this reason, this study proposes a case-based expert system that helps experts overcome the drawbacks in discovering referential markets. First, this study proposes the use of Euclidean distance measure to calculate the similarity between markets. Based on their similarities, markets are grouped into clusters. Then, missing markets with the characteristics of the cluster are searched for. Potential candidate reference markets are extracted and recommended to users. After the iteration of these steps, definite reference markets are determined according to the user's selection among those candidates. Then, finally, the new market's parameters are estimated from the reference markets. For this procedure, two techniques are used in the model. One is clustering data mining technique, and the other content-based filtering of recommender systems. The proposed system implemented with those techniques can determine the most adjacent markets based on whether a user accepts candidate markets. Experiments were conducted to validate the usefulness of the system with five ICT experts involved. In the experiments, the experts were given the list of 16 ICT markets whose parameters to be estimated. For each of the markets, the experts estimated its parameters of growth curve models with intuition at first, and then with the system. The comparison of the experiments results show that the estimated parameters are closer when they use the system in comparison with the results when they guessed them without the system.