• Nutrition Research and Practice
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• v.10 no.2
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• pp.131-138
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• 2016

BACKGROUND/OBJECTIVES: Citrus and its peels have been used in Asian folk medicine due to abundant flavonoids and usage of citrus peels, which are byproducts from juice and/or jam processing, may be a good strategy. Therefore, the aim of this study was to examine antioxidant and anti-inflammatory effects of bioconversion of Jeju Hallabong tangor (Citrus kiyomi ${\times}$ ponkan; CKP) peels with cytolase (CKP-C) in RAW 264.7 cells. MATERIALS/METHODS: Glycosides of CKP were converted into aglycosides with cytolase treatment. RAW 264.7 cells were pre-treated with 0, 100, or $200{\mu}g/ml$ of citrus peel extracts for 4 h, followed by stimulation with $1{\mu}g/ml$ lipopolysaccharide (LPS) for 8 h. Cell viability, DPPH radical scavenging activity, nitric oxide (NO), and prostagladin $E_2$ ($PGE_2$) production were examined. Real time-PCR and western immunoblotting assay were performed for detection of mRNA and/or protein expression of pro-inflammatory mediators and cytokines, respectively. RESULTS: HPLC analysis showed that treatment of CKP with cytolase resulted in decreased flavanone rutinoside forms (narirutin and hesperidin) and increased flavanone aglycoside forms (naringenin and hesperetin). DPPH scavenging activities were observed in a dose-dependent manner for all of the citrus peel extracts and CKP-C was more potent than intact CKP. All of the citrus peel extracts decreased NO production by inducible nitric oxide synthase (iNOS) activity and $PGE_2$ production by COX-2. Higher dose of CKP and all CKP-C groups significantly decreased mRNA and protein expression of LPS-stimulated iNOS. Only $200{\mu}g/ml$ of CKP-C markedly decreased mRNA and protein expression of cyclooxygenase-2 in LPS-stimulated RAW 264.7 cells. Both 100 and $200{\mu}g/ml$ of CKP-C notably inhibited mRNA levels of $interleukin-1{\beta}$ ($IL-1{\beta}$) and IL-6, whereas $200{\mu}g/ml$ CKP-C significantly inhibited mRNA levels of $TNF-{\alpha}$. CONCLUSIONS: This result suggests that bioconversion of citrus peels with cytolase may enrich aglycoside flavanones of citrus peels and provide more potent functional food materials for prevention of chronic diseases attributable to oxidation and inflammation by increasing radical scavenging activity and suppressing pro-inflammatory mediators and cytokines.

• Journal of the Korean Society of Food Science and Nutrition
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• v.35 no.4
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• pp.410-415
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• 2006

The effects of cheonggukjang (traditional fermented soybean food, CK) and cheonggukjang added Phellinus linteus (CKP) on lipid metabolism were investigated in adult male rats. Twenty weeks old Sprague-Dawley rats were fed a purified hyperlipidemic diet (control diet: 0.5% cholesterol, 10% fat, 18% casein) for 4 weeks. The rats were randomly assigned to each treatment group control, two kinds of CK or CKP (powders of CK or CKP as dietary protein sources) After 5 weeks of CK or CKP diets consumption, the body weights, the hepatic and epididymal fat pad weights of the CK or CKP diets groups were significantly decreased than those of the control group. The liver cholesterol and triglyceride levels were significantly lower in both the CK and CKP diets groups than those in the control group. The concentrations in serum triglyceride, total cholesterol, LDL-cholesterol, and atherogenic index ratios were significantly decreased in the CK and CKP diets groups compared with those in the control group. The HDL-cholesterol/total-cholesterol ratios were significantly increased in the CK and CKP diets groups compared with those in the control group. Fecal cholesterol and triglyceride excretion of the CK and CKP diets groups were significantly increased than those of the control group. These results showed that both the feeding of cheonggukjang and cheonggukjang added Pheilinus linteus decreased the triglyceride, total cholesterol and LDL-cholesterol in serum as well as the triglyceride and cholesterol in liver, and increased the HDL-cholesterol/total-cholesterol ratio in serum of the rats.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.46 no.5
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• pp.520-527
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• 2013

This study developed patties using the Antarctic krill Euphausia superba and investigated the food quality of the patties. Three different types of krill patty were produced: krill patties with added 1) corn oil (CKP), 2) AA grade surimi (AKP), and FA grade surimi (FKP). Their proximate composition, Hunter color value, texture, sensory evaluation, and levels of astaxanthin, cholesterol, calories, total amino acids, fatty acids, and fluoride were investigated. The moisture and crude protein contents were highest in FKP. The calorie and astaxanthin contents were highest in CKP and lowest in AKP. The cholesterol content was highest in CKP and lowest in AKP. Measuring the Hunter color value of cross-sections of the patties, the L values were highest for FKP, and the a and b values highest for AKP. The total amino acids were highest in FKP and lowest in CKP. The major fatty acids were highest in FKP. The hardness and chewiness were highest in FKP and lowest in CKP. The fluoride content was highest in AKP and lowest in FKP. The sensory evaluation had the highest scores for FKP and lowest for CKP. Accordingly, FKP appears to have better food quality than the other two products.

• Journal of Life Science
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• v.19 no.11
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• pp.1679-1683
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• 2009

This study was performed to investigate the effects of Cheonggukjang (traditional soybean food, CK) and Cheonggukjang added with Phellinus linteus myceria (CKP) on the lipid metabolism, growth, food intake, and food efficiency ratio(FER) in adult female rats. Sprague-Dawley rats were fed a high fat diet (control diet: 0.1% cholesterol, 10% Lard) for 4 weeks. The rats were randomly assigned to each treatment group: control, and two kinds of CK and CKP (powders of CK and CKP as dietary protein sources). After 4 weeks of experimental diet consumption, the body weights and the uterine fat pad weights of the CK and CKP diet groups were more significantly decreased than those of the control diet group. The hepatic cholesterol, triglyceride and total lipid levels were significantly lower in both the CK and CKP diet fed groups than those in the control group. The concentrations in serum triglyceride and LDL-cholesterol were significantly decreased in the CK and CKP diet groups compared to those in the control group. Fecal total lipid, moisture and wet weight excretion in the CK and CKP diet groups were more increased than those in the control group. These results showed that feeding of cheonggukjang and cheonggukjang added with Phellinus linteus myceria both the triglyceride and LDL-cholesterol in serum, as well as the triglyceride and cholesterol in the livers of the rats.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2014.10a
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• pp.515-517
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• 2014

엔진 부조(engine hesitation)의 원인을 추적함에 있어 가장 효과적인 점검방법 중의 하나가 엔진파워 밸런스(engine power balance)를 점검하는 것이다. 본 연구에서는 공회전 상태에서 4개의 실린더 폭발의 압력 편차로 인한 엔진 부조를 점검하기 위해 엔진 파워 밸런스를 점검하는 방법을 사용한다. 엔진의 부조는 네 개의 실린더의 회전 밸런스가 틀어졌기 때문이므로 실린더의 회전 밸런스 정보를 수집 분석하는 방법이 필요하다. 본 연구에서는 실린더의 엔진 회전수를 계산하기 위해 OBD-II와 무선 시스템인 블루투스 모듈을 이용하여 실시간으로 CKP 센서 정보를 수집하는 방법과 밸런스가 틀어진 실린더를 찾는 알고리즘을 제시하여 엔진 파워 밸런스를 조절 관리하는 시스템을 스마트 폰에 구현하고자한다.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2012.05a
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• pp.391-394
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• 2012

Recently, for appling to industrial engines to CRDI diesel engine to meet enhanced emission regulatory standards in native and foreign, ECU that be controlled only automaker develop engine control algorithm that adjust injection timing and injection as user's need and use testing, verification for engine performance and emissions reduction. For development only CRDI diesel engine emulator, using CKP and CMP sensor performance property of CRDI engine control ECU input element, in this paper, there determine the diesel knocking and propose design methodology of engine balance correction algorithm design of the correction algorithm. And there propose efficient Improvement for fuel efficiency increasing and reduction of emissions.

• Proceedings of the Korea Information Processing Society Conference
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• 2012.11a
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• pp.1252-1255
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• 2012

CRDI 디젤엔진을 산업용 엔진에 적용하여 최근 강화된 국내외의 배출가스 규제 조건을 충족시키기 위해 사용자가 연료의 분사시기와 분사량을 조절할 수 있는 CRDI ECU 제어 알고리즘을 개발하였다. CRDI 엔진 제어 ECU의 입력 요소 중 CKP와 CMP 센서를 이용하여 디젤 노킹 및 롱투스를 판별하는 알고리즘을 개발하여 분사시기와 분사량의 조절 시점을 결정하고, 엔진의 이상 유무를 판별할 수 있는 알고리즘을 개발하여 엔진의 밸런스를 조절함으로써 연비 향상 및 유해가스 저감을 효율적으로 개선할 수 있도록 하였다.

• Journal of Veterinary Clinics
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• v.18 no.4
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• pp.390-396
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• 2001

CKP isoenzymes have a high level of efficaciousness as diagnostic and prognostic aids in various diseases. There is not any report on the total activity of CPK of non-human primates, let alone CPK isoenzyme patterns, in Korea. In this study, total activities and isoenzyme patterns of CPK were measured to obtain their reference values in domestically reared common marmosets, crab-eating macaques and Japanese macaques. We observed remarkable different values of serum total CPK from the primates used in this experiment. Serum CPK activities of Japanese macaques and crab-eating macaques were 275.8$\pm$158.1 IU/l and 396.7$\pm$697.4 IU/l, respectively, whereas those of common marmosets showed much higher value of 618.8$\pm$1,117.6 IU/l. In all common marmosets and crab-eating macaques, only CPK$_3$ ws observed. In five out of eight Japanese macaques, CPK$_3$ was the sole fraction but two animals showed CPK$_1$ and CPK$_3$ isoenzymes, and the remaining one had CPK$_2$ and CPK$_3$ fractions. There were some discrepancies in the pattern and ratio of isoenzyme fractions in Japanese macaques. In conclusion, values such as CPK and CPK isoenzyme patterns of investigated for the first time form non-human primates reared in Korea, could be reference values for the optimal diagnosis and therapy diseases of the corresponding animal species.

• The Korea Journal of Herbology
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• v.24 no.3
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• pp.51-58
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• 2009

Objectives : This study was conducted to evaluate the antioxidative and anticancer activity of the water and ethanol extracts from pickled soybean products by manufacturing stage. Methods : Yakkong (YK) was pickled for 15 days to prepare Chokong (CK) and Chokong pills (CKP) were mixed Chokong (CK) powder with vinegar. Herbal Chokong pills (HCKP) were made by addition five kinds of medicinal herbs to Chokong. Pickled soybean products by each manufacturing process were extracted with distilled water and 70% ethanol, and the extracts were tested for their antioxidative and anticancer activities. Results : The highest electron donating abilities (EDA) of the water and ethanol extracts were from HCKP among pickled soybean products and those were 81.2% and 91.5% at 1,000 $\mu$g/mL, respectively. The ethanol extracts of pickled soybean products had higher activities of EDA and superoxide dismutase (SOD) than water extracts. The highest anticancer activity was the water extracts of HCKP against both of MDA and A549 cells. Conclusions : These results suggest that herbal Chokong pills (HCKP) can be used as natural antioxidant to prevent oxidative damage in normal cells probably because of their antioxidative characteristics.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.7 no.6
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• pp.1004-1011
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• 2006

In this study, we have performed a systematic case study on troubles and diagnoses of passenger car's engine based on OBD. We have acquired 1,242 data in order to analysis accurate troubles' causes and apposite diagnoses. 128 data of them are got using OBD apparatus, and the rest of them are collected on related website. As results, distribution on trouble cases shows bad idling(32%), poor acceleration(21%), stop in running(19%), faulty start(11%), inferior fuel economy(9%), and insufficient power(8%) in order of magnitude. And in the systematic cases, it is not difficult to detect troubles in a single part. But we know that special apparatus such as multichannel scanner is needed in complicated troubles. Furthermore we think that the survey is continued in various ways for more systematic case study on troubles and diagnoses.