• Title/Summary/Keyword: CHS

Search Result 221, Processing Time 0.03 seconds

A Partial Nucleotide Sequence of Chitin Synthase (CHS) Gene from Rice Blast Fungus, Pyricularia oryzae and Its Cloning

  • Hwang, Cher-Won;Park, In-Cheol;Yeh, Wan-Hae;Takagi, Masamchi;Ryu, Jin-Chang
    • Journal of Microbiology and Biotechnology
    • /
    • v.7 no.2
    • /
    • pp.157-159
    • /
    • 1997
  • A 340-bp chitin synthase gene(CHS) fragment was cloned from the genomic DNA of Pyricularia oryzae using a PCR process with two primer DNAs corresponding to highly conserved sequences within fungal CHS genes. The entire DNA nucleotide sequences of the cloned DNA fragment were determined and analyzed. The amino acid sequences deduced from the nucleotide sequence of the amplified DNA fragment showed 86% homology to that of the Aspergillus fumigatus CHSE gene (9). Using this PCR-amplified DNA, about 2.3 kb of including the PCR fragment of CHSE gene was cloned from genomic library.

  • PDF

Hyperperfusion Syndrome after Carotid Stent-Supported Angioplasty in Patients with Autonomic Dysfunction

  • Kim, Dong-Eun;Choi, Seong-Min;Yoon, Woong;Kim, Byeong C.
    • Journal of Korean Neurosurgical Society
    • /
    • v.52 no.5
    • /
    • pp.476-479
    • /
    • 2012
  • Cerebral hyperperfusion syndrome (CHS) is a rare, serious complication of carotid revascularization either after carotid endarterectomy or carotid stent placement. Although extensive effort has been devoted to reducing the incidence of CHS, little is known about the prevention. Postprocedural hypertension is very rare due to autoregulation of carotid baroreceptors but may occur if presented with autonomic dysfunction. We present two cases of CHS after cerebral revascularization that presented autonomic dysfunction.

Efficient Cluster Radius and Transmission Ranges in Corona-based Wireless Sensor Networks

  • Lai, Wei Kuang;Fan, Chung-Shuo;Shieh, Chin-Shiuh
    • KSII Transactions on Internet and Information Systems (TIIS)
    • /
    • v.8 no.4
    • /
    • pp.1237-1255
    • /
    • 2014
  • In wireless sensor networks (WSNs), hierarchical clustering is an efficient approach for lower energy consumption and extended network lifetime. In cluster-based multi-hop communications, a cluster head (CH) closer to the sink is loaded heavier than those CHs farther away from the sink. In order to balance the energy consumption among CHs, we development a novel cluster-based routing protocol for corona-structured wireless sensor networks. Based on the relaying traffic of each CH conveys, adequate radius for each corona can be determined through nearly balanced energy depletion analysis, which leads to balanced energy consumption among CHs. Simulation results demonstrate that our clustering approach effectively improves the network lifetime, residual energy and reduces the number of CH rotations in comparison with the MLCRA protocols.

Fuzzy Logic Approach to Zone-Based Stable Cluster Head Election Protocol-Enhanced for Wireless Sensor Networks

  • Mary, S.A. Sahaaya Arul;Gnanadurai, Jasmine Beulah
    • KSII Transactions on Internet and Information Systems (TIIS)
    • /
    • v.10 no.4
    • /
    • pp.1692-1711
    • /
    • 2016
  • Energy is a scarce resource in wireless sensor networks (WSNs). A variety of clustering protocols for WSNs, such as the zone-based stable election protocol-enhanced (ZSEP-E), have been developed for energy optimization. The ZSEP-E is a heterogeneous zone-based clustering protocol that focuses on unbalanced energy consumption with parallel formation of clusters in zones and election of cluster heads (CHs). Most ZSEP-E research has assumed probabilistic election of CHs in the zones by considering the maximum residual energy of nodes. However, studies of the diverse CH election parameters are lacking. We investigated the performance of the ZSEP-E in such scenarios using a fuzzy logic approach based on three descriptors, i.e., energy, density, and the distance from the node to the base station. We proposed an efficient ZSEP-E scheme to adapt and elect CHs in zones using fuzzy variables and evaluated its performance for different energy levels in the zones.

Biosynthesis of Pinocembrin from Glucose Using Engineered Escherichia coli

  • Kim, Bong Gyu;Lee, Hyejin;Ahn, Joong-Hoon
    • Journal of Microbiology and Biotechnology
    • /
    • v.24 no.11
    • /
    • pp.1536-1541
    • /
    • 2014
  • Pinocembrin is a flavonoid that exhibits diverse biological properties. Although the major source of pinocembrin is propolis, it can be synthesized biologically using microorganisms such as Escherichia coli, which has been used to synthesize diverse natural compounds. Pinocembrin is synthesized from phenylalanine by the action of three enzymes; phenylalanine ammonia lyase (PAL), 4-coumarate:CoA ligase (4CL), and chalcone synthase (CHS). In order to synthesize pinocembrin from glucose in Escherichia coli, the PAL, 4CL, and CHS genes from three different plants were introduced into an E. coli strain. Next, we tested the different constructs containing 4CL and CHS. In addition, the malonyl-CoA level was increased by overexpressing acetyl-CoA carboxylase. Through these strategies, a high production yield (97 mg/l) of pinocembrin was achieved.

CHS : Cluster Head Self-election algorithm in WSNs (센서 네트워크에서 클러스터 헤드 자가 선출 알고리즘)

  • Choi, Koung-Jin;Jung, Suk-Moon
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
    • /
    • 2009.10a
    • /
    • pp.534-537
    • /
    • 2009
  • Clustering protocol of Wireless sensor networks(WSNs) can not only reduce the volume of inter-node communication by the nodes's data aggregation but also extend the nodes's sleep times by cluster head's TDMA-schedule coordination. In order to extend the network lifetime of WSNs, we propose CHS algorithm to select cluster-head using three variables. It consists of initial and current energy of nodes, round information, and total numbers which have been selected as cluster head until current round.

  • PDF

Buckling analysis of thin-walled circular hollow section members with and without longitudinal stiffeners

  • Cuong, Bui H.
    • Structural Engineering and Mechanics
    • /
    • v.81 no.2
    • /
    • pp.231-242
    • /
    • 2022
  • Numerical solutions for the linear buckling behavior of thin-walled circular hollow section members (CHS) with and without longitudinal stiffeners are presented using the semi-analytical finite strip method (SAFSM) which is developed based on Marguerre's shallow shell theory and Kirchhoff's assumption. The formulation of 3-nodal line finite strip is presented. The CHS members subjected to uniform axial compression, uniform bending, and combination of compression and bending. The buckling behavior of CHS is investigated through buckling curves which relate buckling stresses to lengths of the member. Effects of longitudinal stiffeners are studied with the change of its dimensions, position, and number.

Variants of LYST and Novel STK4 Gene Mutation in a Child With Accelerated Chediak Higashi Syndrome

  • Asrar Abu Bakar;Haema Shunmugarajoo;Jeyaseelan P. Nachiappan;Intan Hakimah Ismail
    • Pediatric Infection and Vaccine
    • /
    • v.31 no.1
    • /
    • pp.122-129
    • /
    • 2024
  • Chediak-Higashi syndrome (CHS) is a rare haematological and immunodeficiency disorder that occurs in childhood leading to recurrent infections, bleeding tendencies and progressive neurological dysfunction. Partial oculocutaneous albinism occurs in almost all cases. The exact prevalence is unknown, and the disease is caused by over 70 identified mutations in the lysosomal trafficking regulator gene. The presence of a bright polychromatic appearance from hair shaft and abnormally large intracytoplasmic granules, especially within neutrophils and platelets in the bone marrow is highly suggestive. Treatment is largely supportive, and the only curative treatment is through an allogeneic hematopoietic stem cell transplant. Without transplant, most patients will enter an accelerated phase of hemophagocytic lymphohistiocytosis (HLH) which carries a high mortality rate. We present a young male with CHS who we had followed through and eventually developed a fulminant accelerated phase. We believe this is only the second reported case of CHS in Malaysia.

Mechanical Response of Changes in Design of Compression Hip Screws with Biomechanical Analysis (생체 역학적 분석에 의한 Compression Hip Screw의 디자인 요소에 대한 평가)

  • 문수정;이희성;권순영;이성재;안세영;이훈
    • Proceedings of the Korean Society of Precision Engineering Conference
    • /
    • 2004.10a
    • /
    • pp.1172-1175
    • /
    • 2004
  • At present, CHS(Compression Hip Screw) is one of the best prosthesis for the intertrochanteric fracture. There is nothing to evaluate the CHS itself with the finite element analysis and mechanical tests. They have same ways of the experimental test of the ASTM standards. The purpose of this study is to evaluate the existing CHS and the new CHS which have transformational design factors with finite element analysis and mechanical tests. The mechanical tests are divided into compression tests and fatigue test for evaluating the failure load, strength and fatigue life. This finite element method is same as the experimental test of the ASTM standards. Under 300N of compression load at the lag screw head. There are less differences between Group (5H, basic type) and Group which has 8 screw holes. However, there are lots of big differences between Group and Group which is reinforced about thickness of the neck range. Moreover, the comparison of Group and Group shows similar tendency of the comparison of Group and Group . The Group is reinforced the neck range from Group. After the experimental tests and the finite element analysis, the most effective design factor of the compression hip screws is the reinforcement of the thickness, even though, there are lots of design factors. Moreover, to unite the lag screw with the plate and to analyze by static analysis, the result of this method can be used with experimental test or instead of it.

  • PDF

Experimental Study of High-strength Steel CHS X-joints Under Axial Compression (지관 압축을 받는 고강도강 X형 원형강관접합부의 구조적 성능에 대한 실험적 연구)

  • Lee, Cheol Ho;Kim, Seon Hu;Chung, Dong Hyun;Kim, Dae Kyung;Kim, Jin Won
    • Journal of Korean Society of Steel Construction
    • /
    • v.29 no.4
    • /
    • pp.291-301
    • /
    • 2017
  • Most of current representative design standards worldwide forbid or impose restrictions on the use of high-strength steels for hollow tubular structures. The mechanical background of these limitations appears unclear and unduly conservative, and their validity needs to be re-evaluated. In this study, a total of 9 CHS(Circular Hollow Section) X-joints were tested under axial compression and analyzed to examine if the high-strength steel restrictions specified by current design standards could be relaxed. All the high-strength steel CHS X-joints tested showed satisfactory performance compared to ordinary steel joints in terms of serviceability, ultimate strength, and ductility, although the yield strength of steel was even as high as 800MPa.