• 한국가구학회지
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• 제26권3호
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• pp.196-206
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• 2015

A South Korea master craftsman had the best technology that oneself and others are certified in various fields. ChoSeokJin coach has continued the lifeline of the Korean wood furniture Jeonbuk region. It is already famous coach to award-winning Olympic coach and tournament of the world. Check his mental world and his life through the work of ChoSeokJin coach. Through a harsh process until the Republic of Korea master craftsman, it was an opportunity to reaffirm once again the importance of effort and commitment. South Korea wood chief coach (the field of furniture) growth phase of the No. 1 ChoSeokJin coach, Ripe stage and spirit such as through his neck furniture fabrication methods, he is him then and the situation until the coach and the intangible cultural heritage had asked We look at the world. The work of ChoSeokJin coach by research analysis separately in the previous fiscal year and late, his hope and neck that is a topic that has been internalized "I want wings that can over time attach to a tree." In his work We understand the passion for furniture.

• Journal of Yeungnam Medical Science
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• 제33권1호
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• pp.68-71
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• 2016

Tumoral calcinosis (TC) is a condition resulting from extensive calcium phosphate precipitation, primarily in the periarticular tissues around major joints. Calciphylaxis is a fatal ischemic vasculopathy mainly affecting dermal blood vessels and subcutaneous fat. This syndrome is rare and predominantly occurs in patients with end-stage renal disease. Here, we report on a rare case involving a patient with TC complicated with calciphylaxis. Our patient was a 31-year-old man undergoing hemodialysis who presented with masses on both shoulders and necrotic cutaneous ulcers, which were associated with secondary hyperparathyroidism, on his lower legs. He underwent subtotal parathyroidectomy, and sodium thiosulfate (STS) was administered for 27 weeks. Twenty months after beginning the STS treatment course, he experienced dramatic relief of his TC and calciphylaxis.

• KSBB Journal
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• 제32권2호
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• pp.90-95
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• 2017

Mammalian cell cultures have been used extensively to produce proteins for therapeutic agent because of their ability to perform post-translational modification including glycosylation. To produce recombinant protein, many factors and parameter are considered such as media composition, host cell type, and culture process. In this study, recombinant human erythropoietin (rhEPO) producing cell line was established by using glutamine synthetase system. To reduce serum concentration in media, we compared direct adaptation with step adaptation. Cell growth was faster in step adaptation. In low-level serum media, there were insufficient glucose for cell growth. Thus, we added glucose in low-level serum media from 2 g/L to 4.5 g/L. Titer of rhEPO was higher than other conditions at 4.5 g/L of glucose. Additionally, N-methyl-D-aspartate (NMDA), 13-cis-retinal, and pluronic F-68 (PF-68) were added to enhance productivity in CHO cell cultures. In conclusion, we applied CHO cell producing rhEPO to low-level of serum in media using step-adaptation. Also, we confirmed positive effect of NMDA, 13-cis-retinal, and PF-68.

• Journal of Periodontal and Implant Science
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• 제31권1호
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• pp.233-242
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• 2001

In the treatment of chronic adult periodontitis, scaling and root planing have been generally performed prior to periodontal flap surgery. The purpose of this study was to evaluate the clinical significance of nonsurgical periodontal therapy prior to periodontal flap surgery in patients with chronic adult periodontitis. Fifty six molars showing bilateral bony defects and 4-6mm periodontal pocket in twelve patients with chronic adult periodontitis were selected. By randomized split-mouth design, in one side, flap operation was performed 4 weeks after scaling and root planing, in the other group, flap operation was only performed without scaling and root planing. Probing pocket depth, gingival recession, clinical attachment level, gingival index, plaque index, and tooth mobility were measured at baseline, before flap operation and post-operation

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2002년도 생물공학의 동향 (X)
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• pp.261-264
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• 2002

CHO (Chinese hamster ovary) cells were transfected with plasmids containing both cis-acting HRE (hypoxia response element) and CMV-promoter that controls tissue-type plasminogen activator (t-PA). CHO cells with HRE produced 16.2 fold higher t-PA concentration than CHO cells without HRE. It was noted that hypoxia strongly induced CHO cell apoptosis. which resulted in decrease of cell viability and protein production. In this study. by introducing Bcl-2, anti-apoptotic gene, we tried to recover cell viability and increase the protein production. When batch culture of both control cells without transfection of Bcl-2 and cells transfected with Bcl-2 were performed in the absence of CoCl ι hypoxia mimic condition. the cells with Bcl-2 were effected specific cell growth rates, maximum cell density. Immunoblotting assay showed Bcl-2 was recombinant with HRE dependent t- P A expression cassette, and their expression level was depended on hypoxia. By introducing Bcl-2, both cell viability and maximum cell density could be increased.

• 한국식생활문화학회지
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• 제26권2호
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• pp.190-197
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• 2011

Chungtaejeon is a traditional tea introduced in the age of the Three States and is the only "Don-cha" culture in the world that survived on the southwestern shore of Korea. To restore Chungtaejeon and to make the tea with consistent quality, the microorganisms involved in traditional type fermentation of Chungtaejeon were isolated, and the tea was prepared with high fermentation ability starters. The sensuous characteristics of Chungtaejeon were also examined. Only Bacilli were found in 3 and 5 year aged Chungtaejeon samples. The Lactobacilli were isolated from properly fermented kimchi and one of them showed high growth capability in media containing green tea extract and also showed strong antagonistic activity against methicillin-resistant Staphylococcus aureus, S. aureus, Salmonella, and E. coli. It was identified and named Lactobacillus plantarum CHO25. Chungtaejeon was fermented with a single starter of L. plantarum CHO25 and with a mixed starter (L. plantarum CHO25, Saccharomyces cerevisiae and Bacillus amyloliquefaciens CHO104). The single fermented sample had the highest cell growth after 5 days of inoculation and the level decreased slowly thereafter. The mixed fermented sample showed strong growth of S. cerevisiae. The highest hunter values were the a value of the single fermented sample and the b value of the mixed sample. The single fermented tea showed the best incense score.

• KSBB Journal
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• 제28권2호
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• pp.86-91
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• 2013

To date, various strategies have been studied to increase specific productivity in Chinese hamster ovary (CHO) cell cultures. Also, albumin-fusion platform is being applied to other important bioactive peptides with short half-lives. Here, we investigated the effects of silkworm gland hydrolysate (SGH) on the production of albumin-erythropoietin (Alb-EPO) in transgenic CHO cells. The viable cell density of CHO cells was increased by 13% in the medium containing 1 mg/mL SGH higher than in the control medium without SGH. In addition, the production of Alb-EPO was also 1.26- fold enhanced by reducing the early apoptosis of CHO cells. In conclusion, SGH could be used as a useful supplement for the enhancement of recombinant protein production.

• 수산해양교육연구
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• 제26권3호
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• pp.567-577
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• 2014

The purpose of this research is to study in depth about the life, activities, and achievements of the swimming gold medalist in Asian Game, Cho O-Ryun, to make systematic correction. This research mainly used literary data to try making theoretical contemplation, and to raise objectivity and substantiality of literary research, and to be devoted to limited literary contents, it used acquaintances who are related to this research, especially his wife, Lee Sung-Lan. Cho O-Ryun's thoughts in physical education can be described as nationalistic physical educational thoughts. He did not only have pride as Korean, and tried to show superiority of our nation to the world, but he also tried very hard to prove that Dok-do Island is a Korean territory and not Japan's. He lived with water all his life, showed his everything as a proud Korean, worked as the most patriotic person of 20thcentury in Korea, and became to be a hero, dream, and hope to people in Korea. Spent all his sweat and energy to our country Korea, Cho O-Ryun, he was this era's true hero.

• 전력전자학회:학술대회논문집
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• 전력전자학회 2001년도 전력전자학술대회 논문집
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• pp.9-13
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• 2001

This paper proposes the electric stimulus tactile apparatus system(TESTAS) loaded on the hap-tic interface using ultrasonic motors(USMs). To touch the virtual object like wall in graphic, the 6 DOF haptic interface provides force feedback to users as if it is real. But the case of sharp virtual object like a puncture, it could not provided the sense of pain, but only the reaction-force. After the TESTAS had been loaded on this haptic interface. it could Provide not only the force but also the pain to users. To estimate the capability of TESTAS, we did experiments of three cases, one was very sharp, another was dull, the other is continuative contact.

• 생약학회지
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• 제19권1호
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• pp.34-38
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• 1988

Veronicastrum species are perennial herb growing on the moisty land. They involves on species (V. sibiricum, Naeng-Cho), one subspecies (V. sibiricum var. zuccarini, Tuel-Naeng-Cho) and one variety (V. sibiricum var. zuccarini for. albiflora). This paper deals with a comparison of the chemical components between Naeng-Cho and Tuel-Naeng-Cho.) In the specified HPLC condition used with ${\mu}BondapakC_{18}\;column$, the retention time of aucubin, catalpol, minecoside and veratryl catalpol ester were 2.30, 2.65, 3.05 and 3.10 min, respectively, and in the other condition used with ${\mu}Bondapak\;C_8-NH_2$, the retention time of aucubin, catalpol, minecoside and veratryl catalpol were 5.4, 5.4, 4.4 and 3.2 min, respectively. The contents of minecoside in Tuel-Naeng-Cho were found to be 3.5 and 1mg/g of dry plant material and that of catalpol were found to be 5 and 2 mg/g, respectively.