• Journal of Animal Science and Technology
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• v.62 no.6
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• pp.801-811
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• 2020

The main objective of this in vitro study was to evaluate red ginseng byproduct (RGP) as a protein resource and its effects on rumen fermentation characteristics, microflora, CO2, and CH4 production in ruminants. Four treatments for in vitro fermentation using buffered rumen fluid over a 48 h incubation period were used: 1, RGP; 2, corn gluten feed (CGF); 3, wheat gluten (WG); and 4, corn germ meal. In vitro dry matter digestibility (IVDMD), in vitro neutral detergent fiber digestibility (IVNDFD), in vitro crude protein digestibility (IVCPD), volatile fatty acids, pH, and ammonia nitrogen (NH3-N) were estimated after 48 h incubation. Gas production was investigated after 3, 6, 12, 24, 36 and 48 h. The CO2 and CH4 were evaluated after 12, 24, 36, and 48 h. A significant difference in total gas production and CO2 emissions was observed (p < 0.01) at all incubation times. CH4 production in RGP were higher (p < 0.05) than that in other treatments but a higher CH4 portion in the total gas production was observed in WG (p < 0.05) at 48 h incubation. The IVDMD, IVNDFD, and IVCPD of RGP was lower than those of other conventional ingredients (p < 0.01). The RGP had the lowest NH3-N value among the treatments (p < 0.01). The RGP also had the lowest total VFA concentration (p < 0.01), but presented the highest acetate proportion and acetate to propionate ratio among the treatments (both, p < 0.01). The abundance of Prevotella ruminicola was higher in RGP than in WG (p < 0.01), whereas RGP has lower methanogenic archaea (p < 0.01). In conclusion, based on the nutritive value, IVDMD, low NH3-N, and decreased methanogenic archaea, RGP inclusion as a protein source in ruminant diets can be an option in replacing conventional feed sources.

• Journal of Sericultural and Entomological Science
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• v.10
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• pp.1-25
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• 1969

Since widely use of anthracite for the radiating purpose in a rearing room in Korea, the weakness of silkworm, poor uniformity of the worm growth, the increase of missing worm ratio, unusual cocoon assorting ratio, and the decrease of cocoon layer ratio have been realized by the author, therefore, he has started out to search the mechanism of these evil influences regarding with various gas densities and terms of the gas treatment which will be supposed to be a warning for temperature control during the silkworm reading work. Among the anthracite gas components, CO or SO$_2$ influence and their complexed harm effect were investigated through the use of anthracite gas, CO and SO$_2$ gas. Meantime, each treatment was applied, separately, during the worm egg stage, young instar, adult instar and whole instar, because the harm intensity for any improperable rearing condition is flexible by the growing stages. Meantime, the gas density and term variation were applied for the above each treatment such as in case of anthracite and CO gas, the following treat methods were used. 1) 0.06% for 30 mins. 2) 0.06% for 1 hour. 3) 0.1% for 1 hour. 4) 0.15% for 1 hour. on the other hand, SO$_2$ gas was used as 1) 120ppm for 30 mins. 2) 200ppm for 30 mins. 3) 400ppm for 30 mins. The measurement of gas density was proceeded by using CO tester (Carbon Monoxide Indicator) and SO$_2$ tester (Indicator Sampler Cgf) which could keep any specific density before each treatment.