• Title/Summary/Keyword: CFX-5

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Comparison of Seven Commercial TaqMan Master Mixes and Two Real-Time PCR Platforms Regarding the Rapid Detection of Porcine DNA

  • Kang, Soo Ji;Jang, Chan Song;Son, Ji Min;Hong, Kwang Won
    • Food Science of Animal Resources
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    • v.41 no.1
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    • pp.85-94
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    • 2021
  • A pig-specific real-time PCR assay based on the mitochondrial ND5 gene was developed to detect porcine material in food and other products. To optimize the performance of assay, seven commercial TaqMan master mixes and two real-time PCR platforms (Applied Biosystems StepOnePlus and Bio-rad CFX Connect) were used to evaluate the limit of detection (LOD) as well as the PCR efficiency and specificity. The LODs and PCR efficiencies for the seven master mixes on two platforms were 0.5-5 pg/reaction and 84.96%-108.80%, respectively. Additionally, non-specific amplifications of DNA from other animal samples (human, dog, cow, and chicken) were observed for four master mixes. These results imply that the sensitivity and specificity of a real-time PCR assay may vary depending on master mix and platform used. The best combination of master mix and real-time PCR platform can accurately detect 0.5 pg porcine DNA, with a PCR efficiency of 100.49%.

Analysis of Seawater Intake System using the RNG k-𝜖 Algorithm (RNG k-𝜖 알고리즘을 이용한 해수취수시스템 분석)

  • Kim, Ji-Ho;Kim, Tae-Won;Lee, Seung-Oh;Park, Young-Jin
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.14 no.12
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    • pp.6447-6454
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    • 2013
  • Seawater intake systems have significant problems due to seawater pollution, suspended solids, unstable intake and maintenance etc. An underground type seawater intake system was newly developed to overcome the existing weaknesses and was facilitated in Gyukpo port. In this study, to check the performance of the new system, the samples for water quality and the 3-D numerical modeling test were conducted. The five times test included the COD, total nitrogen, total phosphorus, pH, and suspended solid for the intake system. The analyses show that the COD, total nitrogen, total phosphorus, PH showedminor changes before and after. On the other hand, the change in suspended solids was significant and water was purified below 5 mg/l, first level fisheries water, after. The numerical model adopted the RNG $k-{\epsilon}$ algorithm and the CFX model based on the finite volume method. The porosity algorithm was used to reproduce filtered-sand, outer diameter, and thickness. The numerical results showed that the double pipe is advantageous in that it provides a uniform pressure between the inner and outer pipe for the flow to be stable. In addition, the use of multiple intake pipes did not interfere with the discharge reduction of 0.98 at the both intake pipes compared with the central intake pipe.

Analysis of the Structural Safety in a Non-heating Greenhouse with a Single Cover for Citrus Cultivation in Jeju (제주지역 감귤재배용 단일피복 무가온하우스의 구조안전성 분석)

  • Yum Sung Hyun;Kim Hak Joo;Chun Hee;Lee Si Young;Kang Yun Im;Kim Young Hyo;Kim Yong Ho
    • Journal of Bio-Environment Control
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    • v.14 no.3
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    • pp.166-173
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    • 2005
  • This study was carried out to evaluate the structural stability in a non-heating greenhouse with a single cover for Citrus cultivation which was built up in Jeju on the basis of the drawing designed by Jejudo Agricultural Research & Extension Services and also to make use of the data for developing a standardized non-heating greenhouse in Jeju. The analysis of a structural stability was conducted by using CFX-5.7 and ANSYS under the design condition of a maximum accumulated snow-depth of 19.1 cm as well as an instantaneous maximum wind velocity of $36.6\;m{\cdot}s^{-1}$ which was set up on the basis of meteorological statistics in Jeju. As a result, the maximum von-Mises stress applied on pipes under the wind velocity of $36.6\;m{\cdot}s^{-1}$ showed a value of $250\;N{\cdot}mm^{-2}$ which was greater than the allowable stress of the pipe with a value of $235.4\;N{\cdot}mm^{-2}$ (=$2,400\;kg{\cdot}cm^{-2}$) and also $53.8\;N{\cdot}mm^{-2}$ under the snow-depth of 19.1 cm, respectively. This result suggested that the greenhouse be unstable under the design condition of an instantaneous wind velocity of $36.6\;m{\cdot}s^{-1}$ so that it was necessary for the greenhouse to be reinforced to secure the structural stability.

CFD Analysis on the Continuous and Variable Damping Characteristics of a Semi-Active Shock Absorber (반능동형 충격흡수기의 연속가변 감쇠특성에 대한 CFD 해석)

  • 윤준원
    • Transactions of the Korean Society of Automotive Engineers
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    • v.12 no.2
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    • pp.101-108
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    • 2004
  • Recently, a semi-active shock absorber has been taking interest because of its low cost and simple structure than the active one. CFD analysis has been conducted to investigate the continuous and variable damping characteristics of the semi-active shock absorber. Also, the flow resistance characteristics of a spool valve has been examined to identify individual parameters(namely, exponent and discharge coefficient) of pressure-flow rate relation needed for the accurate valve modeling. The flow field in the damping valve was simulated using the commercial code, CFX-5.3. The numerical results showed reasonable agreement with the experimental outputs. The pressure distribution with the variation of spool opening length and volume flow rate were discussed in detail. And the continuous and variable damping performance was found clearly. The individual parameters of spool valve were obtained as a function of orifice area. The exponent and discharge coefficient were fitted in with the first and the third polynomial respectively.

A Study on the Flow characteristics of Wells Turbine for Wave Power Conversion by Various Flap Shape (파력발전용 웰즈터빈의 Flap형상변화에 따른 유동 특성에 관한 연구)

  • Kim, Dong-Kyun;Choi, Gab-Song;Kim, Jeong-Hwan
    • Journal of the Korean Solar Energy Society
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    • v.26 no.2
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    • pp.1-7
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    • 2006
  • A numerical investigation was performed to determine the effect of airfoil on the optimum flap height using NACA0015 Wells turbine. The five double flaps which have 0.5% difference were selected. A Navier-Stokes code, CFX-TASCflow, was used to calculate the flow field of the Wells turbine. The basic feature of the Wells turbine is that even though the cyclic airflow produces oscillating axial forces on the airfoil blades, the tangential force on the rotor is always in the same direction. Geometry used to define the three dimension numerical grid is based upon that of an experimental test rig. This paper tries to disign the double flap of Wells turbine with the numerical analysis.

선박용 디젤엔진 SCR 시스템의 효율향상을 위한 점성유동해석

  • Song, Ha-Cheol;Sim, Cheon-Sik;Park, Yun-Yong
    • Proceedings of the Korean Institute of Navigation and Port Research Conference
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    • 2016.05a
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    • pp.88-89
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    • 2016
  • 선택적 환원 촉매(SCR : Selective Catalytic Reduction) 시스템은 대기오염을 예방하기 위한 배기가스 처리장치 중 하나이다. 본 연구에서는 전산유체역학(CFD : Computational Fluid Dynamics)를 사용하여 SCR 시스템 의 효율향상을 위하여 ANSYS-CFX package를 이용하여 점성 유동 해석을 수행하였다. SCR 시스템의 점성 유동 흐름의 전산 유체 역학을 이용하여 시뮬레이션하기 위하여 Navier-Stokes 방정식을 지배방정식으로 사용하였다. CATIA V5를 사용하여 SCR 시스템의 형상을 3D 모델링을 하였고, 암모니아와 배기가스의 혼합 비율을 확인하기 위해 요소수 분사 노즐의 위치를 변경하였다. 요소수 분사 노즐은 배기관의 입구로부터 1/3, 1/2, 2/3에 위치한다. 또한, 분사 노즐의 위치가 배기관 입구의 1/3에 위치할 때 노즐의 분사구수에 따른 효율을 확인하기 위하여 분사구수를 4Hole, 6Hole, 8Hole일 경우를 확인하여 비교하였다. 시뮬레이션의 결과로는 배기관 입구에 가까울수록, 분사구수가 많을수록 효율이 좋아짐을 확인하였다.

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Study on the Characteristics of Heat Transfer with Array of Multiple Impinging Jet Nozzle (충돌제트 노즐의 다중 배열 형상에 따른 열전달 특성)

  • Kim, D.K.;Son, J.D.
    • Journal of Power System Engineering
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    • v.10 no.3
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    • pp.32-37
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    • 2006
  • In this paper, we present the flow and heat transfer characteristics with the array of impinging jet nozzles by using the numerical computation and experiment. Numerical solutions were obtained for dimensionless gap H=6, dimensionless outlet length L=10 and Reynolds number Re=1500 by using the commercial CFD code, CFX-5. Experimental and numerical results were agreed well with each other. It was found that the impinging jet with circular array nozzles generated the uniform heat transfer area and the maximum heat transfer is higher than rectangular array nozzles for certain parameter sets. It is one of the most important utilities providing steam to turbine in order to supply mechanical energy in thermal power plant. It is composed of thousands of tubes for high efficient heat transfer.

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A Study on the Design of Wells Turbine for Wave Power Conversion by Various Flap Shape (1) (플랩현상 변화에 따른 파력발전용 웰즈터빈의 형상설계에 관한 연구(1))

  • Kim D.K.;Kim J.H.;Choi Y,H.;Bae S.T.;Lee Y.W.;Lee Y.H.
    • Korean Journal of Computational Design and Engineering
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    • v.9 no.3
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    • pp.253-259
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    • 2004
  • A numerical investigation was performed to determine the effect of airfoil on the optimum flap height using NACA0015 Wells turbine. The five double flaps which have 0.5% difference were selected. A Navier-Stokes code, CFX-TASCflow, was used to calculate the flow field of the Wells turbine. The basic feature of the Wells turbine is that even though the cyclic airflow produces oscillating axial forces on the airfoil blades, the tangential force on the rotor is always in the same direction. Geometry used to define the three dimension numerical grid is based upon that of an experimental test rig. This paper tries In optimized disign the double flap of Wells turbine with the numerical analysis.

Calculations of Pressure Difference in Orifice Flowmeter using CFD (CFD를 이용한 오리피스 유량계의 차압계산)

  • Kim, Hong-Min;Kim, Kwang-Yong;Her, Jae-Young;Ha, Young-Chul
    • 유체기계공업학회:학술대회논문집
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    • 2001.11a
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    • pp.400-403
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    • 2001
  • In this study, commercial CFD code, i.e, CFX-4.3 is used to analyze the flow field and to calculate pressure differences in an orifice flowmeter. Four numerical schemes and five turbulence models are tested. Hybrid scheme and Reynolds stress model show the best performance. Chosen scheme and turbulence model are applied to predict pressure differences through the orifice for the diameter ratios, 0.3, 0.5, and 0.7. And, the results are compared with the experimental data. The results show that the calculation error is inversely proportional to the diameter ratio, and is proportional to the mass flow rate.

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Development of Electrical and Oil Heater for Energy Saving (에너지 절감형 전기 유류 겸용 온풍기 개발)

  • Chung, Sung-Won;Kim, Dong-Keon;Gong, Sang-Ho
    • Journal of the Korean Society of Manufacturing Process Engineers
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    • v.10 no.5
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    • pp.38-43
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    • 2011
  • This study was carried out to evaluate the structural stability of hybrid type fan heater. The evaluation of structural safety of hybrid fan heater was conducted by using Ansys Workbench and CFX-11 under the design condition. The hybrid fan heater was operated by heat transfer for heat source supplied from electric heater and combustion gas. According to result of structural analysis, the maximum equivalent stress of hybrid fan heater was 150MPa when the temperature of heat transfer fluids was $150^{\circ}C$. It was found that the hybrid fan was structurally safe because the value of maximum equivalent stress was smaller than that of yield stress of the material.