• BMB Reports
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• v.55 no.2
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• pp.104-109
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• 2022

Skeletal myogenesis is essential to keep muscle mass and integrity, and impaired myogenesis is closely related to the etiology of muscle wasting. Recently, miR-141-3p has been shown to be induced under various conditions associated with muscle wasting, such as aging, oxidative stress, and mitochondrial dysfunction. However, the functional significance and mechanism of miR-141-3p in myogenic differentiation have not been explored to date. In this study, we investigated the roles of miR-141-3p on CFL2 expression, proliferation, and myogenic differentiation in C2C12 myoblasts. MiR-141-3p appeared to target the 3'UTR of CFL2 directly and suppressed the expression of CFL2, an essential factor for actin filament (F-actin) dynamics. Transfection of miR-141-3p mimic in myoblasts increased F-actin formation and augmented nuclear Yes-associated protein (YAP), a key component of mechanotransduction. Furthermore, miR-141-3p mimic increased myoblast proliferation and promoted cell cycle progression throughout the S and G2/M phases. Consequently, miR-141-3p mimic led to significant suppressions of myogenic factors expression, such as MyoD, MyoG, and MyHC, and hindered the myogenic differentiation of myoblasts. Thus, this study reveals the crucial role of miR-141-3p in myogenic differentiation via CFL2-YAP-mediated mechanotransduction and provides implications of miRNA-mediated myogenic regulation in skeletal muscle homeostasis.

• Journal of the Korea institute for structural maintenance and inspection
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• v.3 no.1
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• pp.121-128
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• 1999

In this study, the behavior of R/C beams strengthened with carbon fiber laminate (CFL) is analyzed from the test results. CFL is attractive for this application due to its good tensile strength and low weight. Test parameters are the width and the thickness of CFL and repair of damaged specimen. The failure mode and ultimate load are analyzed from these measured data. Test results show that the peak load of specimens strengthened with CFL is increased to 1.27~2.04 times that of non-rehabilitation specimen. The wider lap width, larger amount of CFL, the larger strength is obtained. But the ductile behavior of the rehabilitated specimens is inversely proportional to the CFL thickness.

• Horticultural Science & Technology
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• v.34 no.2
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• pp.207-219
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• 2016

Incandescent (INC) lamps have been commonly used to promote flowering of long-day (LD) plants during short-day (SD) seasons, but production of INC lamps has been prohibited due to their low energy efficiency. One of the light sources replacing INC lamps is a compact fluorescent lamp (CFL). This study was carried out to compare the flowering and morphological responses of LD annuals grown in a controlled environment greenhouse at $20^{\circ}C$ with a truncated 9-h SD and a 2- or 4-h night interruption (NI) or 6-h day extension (DE) provided by lighting from INC lamps, CFLs, or a combination of the two (INC + CFLs), in which red (R) to far-red (FR) ratios were 0.60, 8.46, and 0.91, respectively, and their PPFDs were $2.3{\pm}0.3{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$. After 12 weeks of treatment, $Petunia{\times}hybrida$ 'Wave Purple' plants did not flower under the SD photoperiod whereas 100% flowered under all of the LD treatments. Flowering was more rapid under the INC or INC + CFL lighting treatments compared to CFL and DE, and 4-h NI enhanced flowering compared to 2-h NI. In addition, plants under DE and 4h-NI generally flowered earlier than under 2-h NI. All petunia 'Single Dreams Red' plants flowered within 65 days after treatment, and flowering was hastened by some LD lighting regimens and lamp types. Plants under DE and 4h-NI generally flowered earlier than under 2-h NI INC or INC + CFL compared to FL, and flowering time under INC 6-h DE was earliest. In addition, INC lighting promoted stem elongation of both petunia cultivars. In both pansy (Viola${\times}wittrockiana$) 'Coiossus Yellow' and 'Delta Blue Blotch', LD treatments, especially using INC lamps, promoted flowering whereas the lighting period had little influence on days to flowering. Therefore, INC or INC + CFL with lower R:FR promoted flowering and stem extension and the promoting effect was larger with longer lighting periods. These results suggest that CFLs can be used to provide LDs to promote the flowering of petunia and pansy and to reduce stem elongation, although the promoting effect on flowering is sometimes less than that of INC lamps alone.

• Animal cells and systems
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• v.16 no.4
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• pp.280-288
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• 2012

Targeted degradation of proteins through ubiquitin-mediated proteolysis is an important control mechanism in various cellular processes. The process of ubiquitin conjugation is achieved by three enzyme complexes, among which the ubiquitin ligase complex (E3) is in charge of substrate specificity. The SCF (SKP1-CUL1-F-box) family portrays the largest and the most characterized member of the E3 ligases. For each SCF complex, the ubiquitination target is recognized by the F-box protein subunit, which interacts with the substrate through a unique C-terminal domain. We have characterized a novel F-box protein CFL-1 that represents a single LRR-type F-box (FBXL) in the Caenorhabditis elegans genome. CFL-1 is highly homologous to FBXL20 and FBXL2 of mammals, which are known to regulate synaptic vesicle release and cell cycle, respectively. A green fluorescence protein (GFP)-reporter gene fused to the cfl-1 promoter showed restricted expression around the amphid and the anus. Modulation of CFL-1 activity by RNAi affected the time interval between defecations. RNAi-treated worms also exhibited reduced tendency to form dauer when exposed to daumone. The potential involvement of CFL-1 in the control of defecation and pheromone response adds to the ever expanding list of cellular processes controlled by ubiquitin-mediated proteolysis in C. elegans. We suggest that CFL-1, as a single LRR-type F-box protein in C. elegans, may portray a prototype gene exerting diverse functions that are allocated among multiple FBXLs in higher organisms.

• Magazine of the Korea Concrete Institute
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• v.10 no.6
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• pp.345-352
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• 1998

Compressive strength of concrete compression members strengthend with carbon fiber laminate(CFL) were studied from the test results. Test parameters are spacing, thickness, width, ply and spliced length of carbon fiber laminates. Specimens strength with sheets failed with sudden tensile rupture of the laminate, which indicate very brittle failure mode. Test result shows that closer spacing and to increas thickness of CFL is more effective. But strengthening ratio with 1-ply CFL is more effective than that of specimen with 2-ply CFL. Compressive strength capacity of specimen splied 24cm shows almost similar strength to that of non-spliced specimen. The ultimate load carrying of specimen strengthened with CFL ranges 1.11~1.86 times of that of non-strengthened specimen.

• Korean Journal of Radiology
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• v.22 no.3
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• pp.384-394
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• 2021

Objective: To quantitatively assess biochemical alterations in the cartilage of the subtalar and midtarsal joints in chronic lateral ankle instability (CLAI) patients with isolated anterior talofibular ligament (ATFL) injuries and combined calcaneofibular ligament (CFL) injuries using MRI T2 mapping. Materials and Methods: This study was performed according to regulations of the Committee for Human Research at our institution, and written informed consent was obtained from all participants. Forty CLAI patients (26 with isolated ATFL injuries and 14 with combined ATFL and CFL injuries) and 25 healthy subjects were recruited for this study. All participants underwent MRI scans with T2 mapping. Patients were assessed with the American Orthopedic Foot and Ankle Society (AOFAS) rating system. The subtalar and midtarsal joints were segmented into 14 cartilage subregions. The T2 value of each subregion was measured from T2 mapping images. Data were analyzed with ANOVA, the Student's t test, and Pearson's correlation coefficient. Results: T2 values of most subregions of the subtalar joint and the calcaneal facet of the calcaneocuboid joint in CLAI patients with combined CFL injuries were higher than those in healthy controls (all p < 0.05). However, there were no significant differences in T2 values in subtalar and midtarsal joints between patients with isolated ATFL injuries and healthy controls (all p > 0.05). Moreover, T2 values of the medial talar subregions of the posterior subtalar joint in patients with combined CFL injuries showed negative correlations with the AOFAS scores (r = -0.687, p = 0.007; r = -0.609, p = 0.021, respectively). Conclusion: CLAI with combined CFL injuries can lead to cartilage degeneration in subtalar and calcaneocuboid joints, while an isolated ATFL injury might not have a significant impact on the cartilage in these joints.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.30 no.4
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• pp.143-152
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• 2018

Numerical convergence and stability of TVD schemes have been applied in the FUNWAVE-TVD model were compared. The fourth order accurate MUSCL-TVD scheme using minmod limiter suggested by Yamamoto and Daiguji (1993), the fourth order accurate MUSCL-TVD scheme using van-Leer limiter suggested by Erduran et al. (2005) and the second order accurate MUSCL-TVD scheme using van-Leer limiter in Zhou et al. (2001) were compared. Comparisons of the numerical scheme were conducted with experimental data of Vincent and Briggs irregular wave experiments. In comparison with the fourth order accurate scheme using van-Leer limiter, the fourth order accurate scheme using minmod limiter is less dissipative but required lower CFL condition for stable numerical solution. On the other hand, the scheme using van-Leer limiter required smaller resolution spatial grid due to numerical dissipation, but relatively higher CFL condition can be used compared to the scheme using minmod limiter. In the breaking wave experiments which were conducted using high resolution spatial grid to reduce numerical dissipation, the characteristic of the schemes can be clearly observed. Numerical instabilities and blow-up of the numerical solutions were found in the irregular wave breaking simulation with the scheme using minmod limiter. However, the simulation can be completed with the scheme using van-Leer limiter, but required low CFL condition. Good agreements with the observed data were also observed in the results using van-Leer limiter.

• Human Ecology Research
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• v.62 no.3
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• pp.399-408
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• 2024

Food literacy (FL) refers to the ability to understand and utilize food information. This concept consists of functional (FFL), interactive (IFL), and critical food literacy (CFL). In today's massive information-driven society, FL needs must be paid attention to as a determinant of health along with health literacy. This study sought to identify the FL of Korean adults, and to analyze the association between FL and nutritional status. Data was collected through an online self-reported survey among 1,355 Korean adults aged 19 to 64 years, stratified by gender and age. An χ² -test, student's t-test, ANOVA, analysis of covariance (ANCOVA), and multinomial logistic regression were performed using IBM SPSS 25.0. There were significant differences in the subjects' FFL, IFL, and CFL according to their gender, age, residence area, work type, and monthly income. Furthermore, it was confirmed that subjects with a higher nutrition quotient (NQ) grade also had higher FLs. Additionally, FFL and CFL contributed to improving the NQ grade. This study is meaningful in that it investigated the FL of Korean adults and provided data to identify populations that are particularly vulnerable to a lack of FL. In addition, it was confirmed that FL can have a positive effect on the nutritional status of adults. In conclusion, this study suggests the possibility of using FL as an intervention strategy to improve nutrition.

• Interaction and multiscale mechanics
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• v.3 no.2
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• pp.111-121
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• 2010

Dynamic analysis of nano/micro bio-sensors based on a multiscale atomistic/continuum theory is introduced. We use a generalized atomistic finite element method (GAFEM) to analyze a bio-sensor which has $3{\times}N_a{\times}N_p$ degrees of freedom, where $N_p$ is the number of representative unit cells and $N_a$ is the number of atoms per unit cell. The stiffness matrix is derived from interatomic potential between pairs of atoms. This work contains two studies: (1) the resonance analysis of nano bio-sensors with different amount of target analyte and (2) the dependence of resonance frequency on finite element mesh. We also examine the Courant-Friedrichs-Lewy (CFL) condition based on the highest resonance frequency. The CFL condition is the criterion for the time step used in the dynamic analysis by GAFEM. Our studies can be utilized to predict the performance of micro/nano bio-sensors from atomistic perspective.

• Journal of international Conference on Electrical Machines and Systems
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• v.3 no.2
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• pp.162-168
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• 2014

Use of nonlinear loads, such as power converters, fluorescent lamps and adjustable speed motor drives, is expected to grow rapidly. All of these loads inject harmonic currents. This paper presents the active filtering of the harmonic distortion generated by the compact fluorescent lamps (CFL). The Instantaneous active and reactive power theory (the p-q Theory) is used to design the control of parallel active filter. The control scheme has been verified using Matlab/Simulink with SimPower Systems through a set of simulation tests under different load conditions. Also, the tuning of the active power filter is performed to improve the quality of the electrical power supply.