• Journal of Microbiology and Biotechnology
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• 제13권1호
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• pp.110-118
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• 2003

Pseudomonas syringae pv. actinidiae strains, which cause canker disease in kiwifruit, were collected from kiwifruit orchards in Korea and identified using biochemical and physiological tests. The nucleotide sequences of the 16s rDNA and 16s-23s internally transcribed spacer of the isolates were found to be Identical to those of' the pathotype strain, Kwl 1, of P syringae pv. actinidiae. Remarkably, no coding sequence for phaseolotoxin biosynthesis or phaseolotoxin- resistant ornithine carbamoyltransferase was found by PCR amplification in any of the new Korean isolates of pseudomonas syringae pv. actinidiae, although this was clearly identified in the control pathotype Kwl 1 reference strain. In contrast, three primer sets derived from the coronatine biosynthetic gene cluster and DNA from the Korean strains yielded amplified DNA fragments of the expected size. A sequence analysis of the PCR products revealed that P. syringae pv. actinidiae and the Korean strains of pv. actinidiae contain coronafncate ligase genes (cfl)with identical sequences, whereas their. corR genes exhibited 91% sequence similarity. The production of coronatine, instead of phaseolotoxin, by the Korean strains of P. syringae pv. actinidiae was confirmed by a bioassay using reference pathovars known to produce coronatine and phaseolotoxin. The genes for coronatine biosynthesis in the Korean strains of P. syringae pv. actinidiae were found to be present on plasmids.

• Journal of Mechanical Science and Technology
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• 제15권8호
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• pp.1165-1173
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• 2001

This paper deals with dynamic analysis of Pipeline Inspection Gauge (PIG) flow control in natural gas pipelines. The dynamic behaviour of PIG depends on the pressure differential generated by injected gas flow behind the tail of the PIG and expelled gas flow in front of its nose. To analyze dynamic behaviour characteristics (e.g. gas flow, the PIG position and velocity) mathematical models are derived. Tow types of nonlinear hyperbolic partial differential equations are developed for unsteady flow analysis of the PIG driving and expelled gas. Also, a non-homogeneous differential equation for dynamic analysis of the PIG is given. The nonlinear equations are solved by method of characteristics (MOC) with a regular rectangular grid under appropriate initial and boundary conditions. Runge-Kutta method is used for solving the steady flow equations to get the initial flow values and for solving the dynamic equation of the PIG. The upstream and downstream regions are divided into a number of elements of equal length. The sampling time and distance are chosen under Courant-Friedrich-Lewy (CFL) restriction. Simulation is performed with a pipeline segment in the Korea gas corporation (KOGAS) low pressure system. Ueijungboo-Sangye line. The simulation results show that the derived mathematical models and the proposed computational scheme are effective for estimating the position and velocity of the PIG with a given operational condition of pipeline.

• 한국전산유체공학회지
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• 제19권1호
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• pp.27-33
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• 2014

A new and efficient implicit scheme is proposed to obtain a steady-state solution in time integration and the comparison of characteristics with the approximation ways for the implicit method to solve the incompressible Navier-Stokes equations is provided. The conservative, finite-volume cell-vertex upwind scheme and artificial compressibility method using dual time stepping for time accuracy is applied in this paper. The numerical results obtained indicate that the direct application of Jacobian matrix to the Lower and upper sweeps of implicit LU-SGS leads to better performance as well as convergence regardless of CFL number and true time step than explicit scheme and approximation of Jacobian matrix. The flow simulation around box in uniform flow with unstructured meshes is demonstrated to check the validity of the current formulation.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2000년도 제15차 학술회의논문집
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• pp.448-448
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• 2000

This paper deals with dynamic behaviour analysis for pipeline inspection gauge (PIG) flow control in natural gas pipeline. The dynamic behaviour of the PIG is depending on the different Pressure between the rear and nose parts, which is generated by injected gas flow behind PIG's tail and expelled gas flow in front of its nose. To analyze the dynamic behaviour characteristics such as gas flow in pipeline, and the PIG's position and velocity, mathematical model is derived as two types of a nonlinear hyperbolic partial differential equation for unsteady flow analysis of the PIG driving and expelled gas, and nonhomogeneous differential equation for dynamic analysis of PIG. The nonlinear equation is solved by method of characteristics (MOC) with the regular rectangular grid under appropriate initial and boundary conditions. The Runge-Kuta method is used when we solve the steady flow equations to get initial flow values and the dynamic equation of PIG. The gas upstream and downstream of PIG are divided into a number of elements of equal length. The sampling time and distance are chosen under Courant-Friedrich-Lewy (CFL) restriction. The simulation is performed with a pipeline segment in the Korea Gas Corporation (KOGAS) low pressure system, Ueijungboo-Sangye line. The simulation results show us that the derived mathematical model and the proposed computational scheme are effective for estimating the position and velocity of PIG with different operational conditions of pipeline.

• Journal of Mechanical Science and Technology
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• 제15권9호
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• pp.1302-1310
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• 2001

This paper introduces modeling and simulation results for pipeline inspection gauge (PIG) with bypass flow control in natural gas pipeline. The dynamic behaviour of the PIG depends on the different pressure across its body and the bypass flow through it. The system dynamics includes: dynamics of driving gas flow behind the PIG, dynamics of expelled gas in front of the PIG, dynamics of bypass flow, and dynamics of the PIG. The bypass flow across the PIG is treated as incompressible flow with the assumption of its Mach number smaller than 0.45. The governing nonlinear hyperbolic partial differential equations for unsteady gas flows are solved by method of characteristics (MOC) with the regular rectangular grid under appropriate initial and boundary conditions. The Runge-Kuta method is used for solving the steady flow equations to get initial flow values and the dynamic equation of the PIG. The sampling time and distance are chosen under Courant-Friedrich-Lewy (CFL) restriction. The simulation is performed with a pipeline segment in the Korea Gas Corporation (KOGAS) low pressure system, Ueijungboo-Sangye line. Simulation results show us that the derived mathematical model and the proposed computational scheme are effective for estimating the position and velocity of the PIG with bypass flow under given operational conditions of pipeline.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2011년도 추계학술대회 초록집
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• pp.91.1-91.1
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• 2011

고체산화물 연료전지(Solid Oxide Fuel Cell이하 SOFC)는 연료가 갖는 화학에너지를 연소과정 없이, 공기와 H2, CO, CH4와 같은 환원성 가스를 공급받아 $600{\sim}1000^{\circ}C$에서 전기화학적 반응을 통하여 직접 전기를 얻는 방식이다. SOFC는 $700^{\circ}C$ 이상의 고온에서 고체산화물이 연료와 공기가 반응하여 전기와 열을 동시에 생산하기 때문에 carnot cycle의 제한을 받지 않아 발전효율이 40% 이상으로 고효율이고, NOx 및 SOx를 배출하지 않아 무공해이며, moving parts가 없어 소음이 나지 않고, 건설과 증설이 지역이나 기후 조건에 제약 없이 용이하고, 다양한 용량이 가능하며, 고가의 백금 촉매를 사용하지 않으며, 수소, 석탄가스, 천연가스 등의 연료를 사용할 수 있는 장점이 있음, 또한 다향한 형태로 제작할 수 있으며 전해질이 고체에서 전해질 손실 및 보충에 문제가 없고 타 연료전지에 비해 개질기가 필요 없어 발전시스템이 간단하고 경량화가 가능하다. 전사법은 paste를 제작하여 전사용지에 Screen printing하여 건조 후 coating하는 방법으로 기존의 여러 coating 방법보다 제작이 용이하고 소재의 크기, 두께조절이 간편하며, 구성층의 표면조도나 굴곡에 대응이 용이한 방법이다. 본 실험에서는 paste 제조, 전사법을 이용하여 Anode, AFL, Electrolyte, CFL, Cathode전사지를 제작하고 이를 세라믹 평관형 지지체에 변수로 두께 조건별 Coating 한 후 $1400^{\circ}C$ 소결을 진행하여 SEM 분석으로 미세구조 관찰, 출력특성 및 Impedance을 확인하였다.

• The Plant Pathology Journal
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• 제33권4호
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• pp.351-361
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• 2017

Bacterial canker is the largest limiting factor in the cultivation and production of kiwifruit worldwide. Typical symptoms comprise necrotic spots on leaves, canker and dieback on canes and trunks, twig wilting, and blossom necrosis. Pseudomonas syringae pv. actinidiae (Psa), which is the causal agent of kiwifruit bacterial canker, is divided into four biovars based on multilocus sequence analysis of different genes, additional PCR testing of pathogenic genes (argKtox cluster, cfl, and various effector genes), and biochemical and physiological characterization. Bacterial canker caused by Psa biovar 2 designated Psa2 was detected for the first time on the green-fleshed kiwifruit cultivar Hayward in 1988 and the yellow-fleshed kiwifruit cultivar Hort16A in 2006 in Korea. Psa biovar 3 designated Psa3, responsible for the current global pandemics of kiwifruit bacterial canker, began to appear in Korea in 2011 and caused tremendous economic losses by destroying many vines or orchards of yellow-fleshed kiwifruit cultivars in one or several growing seasons. Bacterial canker epidemics caused by both Psa2 and Psa3 are prevalent in Korea in recent years. In this review, we summarize the symptomatology, etiology, disease cycle, diagnosis, and epidemiology of kiwifruit bacterial canker in Korea.

• Neonatal Medicine
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• 제28권2호
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• pp.89-93
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• 2021

Nemaline myopathy is a genetically heterogeneous neuromuscular disorder and one of the most common congenital myopathies. The clinical manifestations usually vary depending on the age of onset. Neonatal nemaline myopathy has the worst prognosis, primarily due to respiratory failure. Several genes associated with nemaline myopathy have been identified, including NEB, ACTA1, TPM3, TPM2, TNNT1, CFL2, KBTBD13, KLHL40, KLHL41, LMOD3, and KBTBD13. Here, we report a neonatal Korean female patient with nemaline myopathy carrying compound heterozygous mutations in the gene KLHL40 as revealed using next generation sequencing (NGS). The patient presented with postnatal cyanosis, respiratory failure, dysphagia, and hypotonia just after birth. To identify the genetic cause underlying the neonatal myopathy, NGS-based gene panel sequencing was performed. Compound heterozygous pathogenic variants were detected in KLHL40: c.[1405G>T];[1582G>A] (p. [Gly469cys];[Glu528Lys]). NGS allows quick and accurate diagnosis at a lower cost compared to traditional serial single gene sequencing, which is greatly advantageous in genetically heterogeneous disorders such as myopathies. Rapid diagnosis will facilitate efficient and timely genetic counseling, prediction of disease prognosis, and establishment of treatments.

• Structural Monitoring and Maintenance
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• 제10권4호
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• pp.299-314
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• 2023

Long-gauge carbon fiber line (CFL) sensors have received considerable attention in the past decade. However, there is still a need for an in-depth investigation of their measuring accuracy. This study investigates the accuracy of carbon fiber line sensors to monitor and differentiate the flexural behavior of two beams, one reinforced with steel bars alone and the other reinforced with steel and basalt fiber-reinforced polymer bars. A distributed set of long-gauge carbon fiber line, Fiber Bragg Grating (FBG), and traditional strain gauge sensors was mounted on the tensile concrete surface of the studied beams to compare the results and assess the accuracies of the proposed sensors. The test beams were loaded monotonically under four-point bending loading until failure. Results indicated the importance of using long-gauge sensors in providing useful, accurate, and reliable information regarding global structural behavior, while point sensors are affected by local damage and strain concentrations. Furthermore, long-gauge carbon fiber line sensors demonstrated good agreement with the corresponding Fiber Bragg Grating sensors with acceptable accuracy, thereby exhibiting potential for application in monitoring the health of large-scale structures.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2009년도 학술발표회 초록집
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• pp.775-779
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• 2009

최근 이상기후 및 국지성 돌발호우 등 여러 가지 형태의 예기치 못한 기상이변으로 인하여 매년 수재해는 빈번히 발생하고 있으며, 이를 위해 하천에서 실시간으로 신속하고 안정성있는 수리학적 하도추적모형의 구축은 지속적으로 연구해야 할 사항이다. 현재 주요 수계 홍수통제소에서는 홍수예보를 위해 주로 수리학적 및 수문학적 모형이 병행되어 적용되고 있으나, 한강과 금강하류를 제외한 나머지 수계에서는 아직까지 개념적 수문학적 모형만을 이용하여 홍수예보를 수행하고 있는 실정이다. 그러나, 신속하고 안정적인 수문학적 모형이 가지고 있는 몇가지 장점에도 불구하고, 실시간 분석 및 신속한 상황대처를 위해서는 정교한 하천흐름해석 기술인 수리학적분석이 반드시 필요하다. 그러나, 대부분의 모형운영자가 직면하게 되는 수리학적 하도추적모형에서 발생하는 수치적 불안정성인 발산의 문제점은 상당한 어려움으로 작용하고 있다. 이는 다양한 원인들이 있을 수 있으나, 대표적으로 단면의 불규칙성을 고려할 수 있다. 실제단면들을 모형에 반영할 때 수치계산의 과정 중에 급확대/급축소/특이단면에 따른 잦은 발산이 발생하게 되며, 이를 방지하기 위해서 단면의 보간 및 평활화 작업 등을 수행하게 된다. 이때 단면의 형상을 최대한 반영한 보간 및 평활화 작업이 되지 않으면, 물리적 개념이 무시된 비합리적인 계산이 수행될 수 있다. 발산의 요인을 제거한 최적의 단면형태를 선정하는 것은 모형의 안정성을 확보하는 데 중요한 요인이 된다. 또한 모형수행에 있어 발산의 요인으로서 하구와 만나게 되는 지점에서의 경계조건으로서 조위영향이 있다. 수리학적 하도추적모형의 중요한 요인인 하구에서의 흐름을 조위와 연계하여 가장 합리적인 하류 경계조건을 제시하는 것이 모형의 발산 방지 및 정확도를 향상시키는데 중요한 인자로 작용하고 있다. 본 연구에서는 이와 같은 수리학적 하도추적모형의 안정성 검증을 위하여, 아직까지 수리학적 하도추적모형이 구축되어 있지 않는, 금강상류구간인 용담댐$^{\sim}$대청댐구간을 설정하여 수리학적 모형의 입력자료를 구축하고, 그에 따른 영향검토를 지속적으로 추진할 계획이다. 대상구간에서 향후 검증될 다양한 수리학적 안정화 기술은 향후 타 수계에서 적용시, 신속하고 합리적인 입력자료 구축에 많은 도움을 줄것이며, 현재 하천에서 발생하는 계산의 불안정성을 빠르게 수정하는 것이 가능하다.