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Studies on the Isolation and Utilization of Apple Wine Yeasts (우수 사과주효모(酒酵母)의 분리(分離)와 이용(利用)에 관(關)한 연구(硏究))

  • Park, Yoon-Joong;Kim, Chan-Jo;Lee, Suk-Kun;Oh, Man-Jin;Sohn, Cheon-Bae
    • Korean Journal of Agricultural Science
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    • v.5 no.1
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    • pp.35-41
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    • 1978
  • Extensive selection works on wild yeasts of fruits were carried cut to obtain strains which are applicable to apple wine making. Among the total number of 1,358 yeast strains which were isolated from various fruit samples collected from the vicinity of Daejeon and other regions cf Korea, the strains SH-49, SH-129 and SH-338 were found to be useful. Then experiments on their morphological and physiological characteristics, and on the aspects of practical use in apple wine making were proceeded. The results obtained were as follows: 1. The strains SH-49 and SH-129, particularly SH-49, were appeared to have good fermentation ability, tolerance to sulfur dioxide and to produce fine quality of apple wine. 2. Apple wines made by using the strain, SH-49 and SH-129 contained less amount of total acids than those by other strains. 3. Apple wines of SH-49 and SH-129 were clarified rapidly during the primary fermentation period, and their absorbancy at 430 nm after 45 days of storage were approximately half of others. 4. Apple wine of SH-338 contained higher amount of residual sugar and its quality was superior to others. It is considered that this strain could be used in the production of apple wine of a characteristic quality. 5. The strains SH-49 and SH-338 were identified as a Saccharomyces cerevisiae according to Taxanomic Study of Yeasts by Lodder, however, classification of SH-129 was suspended for further study.

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Effects of Harvest Date and Cultivar on the Growth, Forage Yield and Quality of Spring Sown Oats at the Middle Mountain Area (중산간지에서 수확시기와 품종이 춘파 연맥의 생육특성, 사초수량 및 사료가치에 미치는 영향)

  • Kim, W.H.;Seo, S.;Jeong, K.H.;Kim, J.G.;Shin, D.E.;Shin, J.S.
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.19 no.1
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    • pp.89-94
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    • 1999
  • This experiment was carried out to determine harvest date and cultivar effects on growth characteristics, forage yield and quality of spring sown oats at the middle mountain(450m) area at the forage experimental field, Namweon Branch, National Livestock Research Institute. The experiment was arranged in a split plot design with three replication. The main plot consisted of the harvest date(9 June, 18 June). The subplots consisted of different maturities of oat cultivars such as Cayuse, Swan, Foothill, Cashel, Martlock and Winjardie. The results obtained are summarized as follows; A period of 50 days was required to be first headed from seeding with early maturity oats(Swan), but that of 77 days was required with late maturity(Foothill). The dry matter content of early maturity(Swan) oats at 9 June and 18 June were 24.01% and 35.69%, but that of late maturity cultivars(Foothill) were 14.02% and 22.84%. The fresh yield of late maturity(Foothill) oats at 9 June and 18 June were 62,666kg and 59,666kg, but that of early maturity(Cashel) were 54,222kg and 45,493kg(P<0.05). The dry yield of early maturity (Cashel) oats at 9 June was 10,169kg, but that of early maturity (Martlock) was 6,272kg. But no significant difference was found among cultivars at June 18. Crude protein content of oats were decreased from 14.0% to 11.1% as the growing stage progressed, ADF, NDF and CF contents were increased. And in vitro dry matter digestibility was decreased as the harvest date delayed. The present experiment indicated that spring sown oats(Foothill) can be successfully produced as fresh forage by seeding in middle March and harvesting in 10 and 20 June at the middle mountain (450m) area.

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Study on the Recycled Compositing System for Reducing Bulking Agent Cost (부자재 비용절감을 위한 순환퇴비화 시스템에 관한 연구)

  • 최명환;홍지형;박금주;최원춘
    • Journal of Animal Environmental Science
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    • v.6 no.3
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    • pp.191-199
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    • 2000
  • This study was initiated to investigate the influence of biophysical condition on the composting characteristics, and conducted to develop technology for using recycled compost as a bulking agent cost to reduce operating cost. To methods of aeration, continuous aeration (CA: run No. I) and intermittent aeration (IA: run No. 2) were performed with three 12.3 liter laboratory scale vessels for ten days. Manure and rice hulls were mixed for thirst trial (I), rice hulls and recycled compost after first trial were mixed for second trial (II), dairy manure and only recycled compost after second trial were mixed for third trial (III). During the composting process, temperatures of the compost mass and ammonia emissions were measured. The quality and maturity of compost were ascertained by examining the characteristics and composition of the compost. Also, loss of mass was determined by measuring the mass of materials in the vessels before and after composting. The results in this study are as follows: 1. The periods of optimum temperature ($>55^{\circ}C$) to kill pathogens were maintained from 38 to 78 hours for CA and from 60 to 98 hours for IA. 2. The more recycled compost mixed, the more ammonia emitted. The maximum ammonia emissions were 287 ppm at CA and 420 ppm at IA. 3. Biofiltration system was required for the compo sting system using only recycled compost as an amendment, because the ammonia emissions was produced above 100 ppm at the end of composting process. 4. The quality and maturity of compost: - Fresh compost, were required drying, because moisture contents of the compost were approximately 70% in all tests. - The pH values were observed to rise smoothly, from 7.9 to 8.3 at CA and from 8.4 to 8.6 at IA. The CfN ratios of the fresh compost were ranged form 21.05 to 16.42 for CA and from 22.81 to 14.75 for IA. The final C/N ratios for test II and III were below 20.were below 20.

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Reproductive Ecology of the Silver Pomfret Pampus argenteus on the West Coast of Korea (한국 서해산 병어, Pampus argenteus의 번식생태)

  • Chung, Ee-Yung;Bae, Joo-Seung;Kang, Hee-Woong;Lee, Hwang-Bok;Lee, Ki-Young
    • Development and Reproduction
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    • v.12 no.2
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    • pp.169-181
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    • 2008
  • Reproductive ecology of the silver pomfret, Pampus argenteus were investigated by histological observations and morphometric data. Samples were collected by the stow net at the coastal area of Jaun-Do, Muan-gun, Korea, from January to December, 2006. P. argenteus is dioecious, the ovary is composed of many ovarian lobules, showing a pair of saccular structure, and the testis is composed of many seminiferous lobules, showing a pair of lobular structure. From February (growing stage) to September (after spawning), monthly changes in the gonadosomatic index, hepatosomatic index, and condition factor in females and males showed similar patterns with the gonad developmental phases. Judging from the results of their indice, it is assumed that spawning in females and males occur from May to July. The reproductive cycle can be classified into five successive stages in females: early growing stage (February to March), late growing stage (March to April), mature stage (March to July), ripe and spent stage (May to July), and recovery and resting stage (July to February); in males, the cycle can be divided into four successive stages: growing stage (February to April), mature stage (March to June), ripe and spent stage (May to July), and recovery and resting stage (July to February). According to the frequency distributions of egg diameters in the breeding season, P. argenteus is presumed to be spring-summer spawning species and polycyclic species to spawn 2 times or more during one spawning season. Number of total eggs in absolute fecundity were proportional to body length and body weight, respectively. Number of total eggs in absolute fecundity per body weight were also proportional to the body length, but if the increase of body weight considerably increased, rather total eggs in relative fecundity decreased with the increase of body weight. Percentage of first sexual maturity of P. argenteus were over 50% in females and males of 12.1 to 15.0 cm in body length, and 100% for fishes over 18.1 cm in length. Therefore, both sexes were regarded to be sexually mature at one year of age.

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Decomposition of 2,4,6-Trinitrotoluene (TNT) by Gamma Ray Irradiation (감마선 조사에 의한 2,4,6-Trinitrotoluene (TNT)의 분해)

  • Lee, Byung-Jin;Lee, Myun-Joo
    • Journal of Korean Society of Environmental Engineers
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    • v.27 no.1
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    • pp.1-10
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    • 2005
  • The purpose of this study was to evaluate the potential of a gamma irradiation to decompose 2,4,6-trinitrotoluene(TNT) in an aqueous solution. The decomposition reaction of TNT by gamma irradiation was a pseudo first-order kinetic over the applied initial concentrations($25{\sim}100mg/L$). The dose constant was strongly dependent on the initial TNT concentration. The removal of TNT was more efficient at pH below 3 and at pH above 11 than at neutral pH(pH 5-9). The required irradiation dose to remove over 99% of TNT was 40, 80 and 10 kGy, individually at pH 2, 7 and 13. The dose constant was increased by 1.6 fold and over 15.6 fold at pH 2 and 13, respectively, when compared with that at pH 7 When irradiation dose of 200 kGy was applied, the removal efficiencies of TOC were 91, 46 and 53% at pH 2, 7 and 13, respectively. Ammonia and nitrate were detected as the main nitrogen byproducts of TNT and glyoxalic acid and oxalic acid were detected as organic byproducts. The results showed that a gamma irradiation was an attractive method for the decomposition of TNT in an aqueous solution. However, regarding the application of high energy radiation for the TNT decomposition and mineralization, an application of an acidic pH below 3 to the solution before irradiation should be considered.

Strength and Deformation Capacities of Short Concrete Columns with Circular Section Confined by GFRP (GFRP로 구속된 원형단면 콘크리트 단주의 강도 및 변형 능력)

  • Cho, Soon-Ho
    • Journal of the Korea Concrete Institute
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    • v.19 no.1
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    • pp.121-130
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    • 2007
  • To investigate the enhancement in strength and deformation capacities of concrete confined by FRP composites, tests under axial loads were carried out on three groups of thirty six short columns in circular section with diverse GFRP confining reinforcement. The major test variables considered include fiber content or orientation, wrap or tube type by varying the end loading condition, and continuous or discontinuous confinement depending on the presence of vortical spices between its two halves. The circumferential FRP strains at failure for different types of confinements were also investigated with emphasis. Various analytical models capable of predicting the ultimate strength and strain of the confined concrete were examined by comparing to observed results. Tests results showed that FRP wraps or tubes provide the substantial increase in strength and deformation, while partial wraps comprising the vertical discontinuities fail in an explosive manner with less increase in strength, particularly in deformation. A bilinear stress-strain response was observed throughout all tests with some variations of strain hardening. The failure hoop strains measured on the FRP surface were less than those obtained from the tensile coupons in all tests with a high degree of variation. In overall, existing predictive equations overestimated ultimate strengths and strains observed in present tests, with a much larger scatter related to the latter. For more accuracy, two simple design- oriented equations correlated with present tests are proposed. The strength equation was derived using the Mohr-Coulomb failure criterion, whereas the strain equation was based on entirely fitting of test data including the unconfined concrete strength as one of governing factors.

Effect of the Various Recovery Diets on the Body Composition of Protein-depleted Rat (각종 회복식이가 무단백식이를 섭취한 흰쥐의 체구성 성분에 미치는 영향)

  • Choi, Young-Ran;Suh, Dong-Hee;Kim, Mi-Kyung;Kim, Sook-He
    • Journal of Nutrition and Health
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    • v.10 no.1
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    • pp.1-9
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    • 1977
  • The changes of the total body composition, internal organs, skeletal muscles, and epididymal fat pad in rats fed protein depletion diet and 4 different recovery diets were examined. Seventy-eight male Sprague-Dawley rats weighing $212{\pm}2$ gr were used, and the results were as follows : A) After the 2 weeks of protein depletion, body weight decreased about 20% from the initial weight. It was mainey due to the total body lipid reduction. Among various organ weights, liver and spleen reduced $35{\sim}58%$, kidney and heart reduced $18{\sim}30%$, and muscles reduced $2{\sim}13%$, while brain, epididymal fat pad were not changed significantly. In regarding protein and lipid contents of these tissues, protein in liver, lipid in muscle, and both in spleen were markedly reduced. B) With the 2 weeks of feeding recovery diets, the increases of body weight were different among 4 Groups. High-fat group gained at the highest level (67%), and high-CHO group the lowest (30%). Total body composition (%) cf the standard and high-protein groups recovered to the level of 0 day protein depletion, while protein in the high-fat group and water in the high-CHO group decreased, and fat in these 2 groups increased. Weights of organs and muscles of the high-protein and high-fat groups were similar to the standard group and tllose of the high-CHO group were lower than the standard group. Composition of organs and muscle in the high-protein group was similar to the standard group, while the N contents of the high-fat and high-CHO groups were lower an the lipid content of the high-fat group was higher than the standard group. The weight and lipid content of epididymal fat pad were the highest in the high-fat group.

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Effects of Low-power Laser and TDP on the Cutaneous Wound Healing (피부상처 치유에 대한 저에너지레이저와 특정전자파의 효과)

  • Ahn So-Youn;Park Sang-Ock
    • The Journal of Korean Physical Therapy
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    • v.9 no.1
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    • pp.1-18
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    • 1997
  • The purpose of this study is to investigate the effects of the low power Helium Neon-Infra Red(HeNe-IR) laser and the special electromagnetic therapeutic apparatus stimulation, which is usually designated as TDP by using the initial of Tending Diancibo Pu which is the Chinese inscribed with English, on wound healing in rat. The seventy-five Sprague-Dawley adult female and male rats were assigned to the experimental and control groups. Each rat was anesthetized with pentobarbital sodium, and three full-thickness incisions with 12mm length wert made on the back of the half cf the rats and three deep second degree burns were made on the back of the remainder of the rats. From 34 hours after being injured, the rats of the experimental laser group were irradiated with the 157mW electric power HeNe-IR laser for 2 minutes every day and the rats of the experimental TDP group were stimulated with TDP irradiation with the 4km spot distance for 20 minutes every day during the 17 days. The rats were sacrificed and the wound parts of its were incised on the first day, 4th day, 7th day, 10th day and 17th day respectively after the beginning of wound treatment with laser and TDP irradiation. The incised wound parts were processed appropriately for the light microscopic and electron microscopic examination. The length of incised wound was measured with microcaliper before the wound part was incised. There was a significant decrease in the length of the incised wound of the experimental laser and TDP group, compared with that of control group at 4th day, 7th day and 10th day(p<0.01) after surgery. Through the histological examination of the wound site, the more rapid epithelialization and collagen formation in experimental groups were showed, compared with control group. The histological results were analyzed and summarized as the follows; The epidermis begins to be regenerated and the granulation tissue begins to be changed to the mature pattern in the H-E stained incised skin of the laser and TDP treatment group on the 4th day. The epidermis shows the complete regeneration and the granulation tissue in the dermis in mostly to be matured in the laser and TDP treatment group on the 7th day, compared with control group. The chronic inflammatory cells are oberved and the necrosis of the collagen fibers are partially observed in control group on the 10th day. The dermis of the laser and TOP treatment group reveals relatively compactly arranged collgen bundles with the mature collagen fibers on the 10th day. The epidermis and dennis of the laser and TDP group are repaired normally and the hair follicles are well regenerated on the 17th day. The mild edema and the granulation tissue is observed in the dermis of the control groups and the delayed treatment process is observed on the 17th day. The Most of proliferated collagen fibrils are found to be compact and regular in electron micrograph of burn skin of the laser treatment group on the 10th day hut the interstitial eadema and some inflammatory cells are found in the control group. The above results suggest that through the visual and histological examination the epithelized epithelium and the proliferation of the collagen liters in the dermis occur very effectively with the low power laser treatment and the TDP treatment in the incised wound healing and the burn wound healing.

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A Meta-analysis of Ambient Air Pollution in Relation to Daily Mortality in Seoul, $1991\sim1995$ (메타분석 방법을 적용한 서울시 대기오염과 조기사망의 상관성 연구 (1991년$\sim$1995년))

  • Dockery, Douglas W.;Kim, Chun-Bae;Jee, Sun-Ha;Chung, Yong;Lee, Jong-Tae
    • Journal of Preventive Medicine and Public Health
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    • v.32 no.2
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    • pp.177-182
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    • 1999
  • Objectives: To reexamine the association between air pollution and daily mortality in Seoul, Korea using a method of meta-analysis with the data filed for 1991 through 1995. Methods: A separate Poisson regression analysis on each district within the metropolitan area of Seoul was conducted to regress daily death counts on levels of each ambient air pollutant, such as total suspended particulates (TSP), sulfur dioxide $(SO_2)$, and ozone $(O_3)$, controlling for variability in the weather condition. We calculated a weighted mean as a meta-analysis summary of the estimates and its standard error. Results: We found that the p value from each pollutant model to test the homogeneity assumption was small (p<0.01) because of the large disparity among district-specific estimates. Therefore, all results reported here were estimated from the random effect model. Using the weighted mean that we calculated, the mortality at a $100{\mu}g/m^3$ increment in a 3-day moving average of TSP levels was 1.034 (95% Cl 1.009-1.059). The mortality was estimated to increase 6% (95% Cl 3-10%) and 3% (95% Cl 0-6%) with each 50 ppb increase for 9-day moving average of SO2 and 1-hr maximum O3, respectively. Conclusions: Like most of air pollution epidemiologic studies, this meta-analysis cannot avoid fleeing from measurement misclassification since no personal measurement was taken. However, we can expect that a measurement bias be reduced in a district-specific estimate since a monitoring station is hefter representative cf air quality of the matched district. The similar results to those from the previous studios indicated existence of health effect of air pollution at current levels in many industrialized countries, including Korea.

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Effects of the Additives on the Quality of Alfalfa Silage (첨가제 처리가 알팔파 사일리지의 품질에 미치는 영향)

  • Kim, J.G.;Chung, E.S.;Kang, W.S.;Ham, J.S.;Kim, J.D.;Seo, S.;Lee, J.K.
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.19 no.2
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    • pp.115-120
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    • 1999
  • This experiment was carried out to investigate the effects of additives on the quality of alfalfa(Medicago sativa L.) silage at the forage experimental field, National Livestock Research Institute, RDA, Suwon in 1996. The experiment was arranged in a randomized block design with three replications. The treatments consisted of different additives(control, formic acid, molasses, inoculant A and inoculant B). Crude protein(CP) and Nitrogen free extract(NFE) content of alfalfa silage with additives were higher compared with those obtained control, but Crude ash(CA) and Crude fiber(CF) content of control(11.5 and 39.6%) were higher than those of additive treated plots. ADF and NDF contents of control silage were the highest as 36.2 and 48.6%, respectively, and increased by 4% compared with those obtained before ensiling. The acidity(pH) of control was the highest(5.45), but the lowest in inoculant A(4.32). Inoculant teratment significantly reduced acidity of silage compared with control. DM content of control silage was the lowest but DM loss of control silage was higher than that of additive treated silage. Ammonia-N content was the highest at formic acid treatment and level of Lactobacillus was decreased at control and formic acid treated silage. Total organic acid and lactic acid content of inoculant A and inoculant B were higher than those of other treated plots. The results of this study indicate that the treatment of LAB(Lactic acid bacteria) could be recommended as producing high quality of alfalfa silage.

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