• Journal of Sericultural and Entomological Science
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• v.52 no.1
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• pp.45-51
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• 2014

A cecropin-A gene was isolated from the immunized larvae of the Japanese oak silkworm, Antheraea yamamai and designed Ay-CecA. The complete Ay-CecA cDNA consists of 419 nucleotides with 195 bp open reading frame encoding a 64 amino acid precursor that contains a putative 22-residue signal peptide, a 4-residue propetide and a 37-residue mature peptide with a theoretical mass of 4046.81. The deduced amino acid sequence of the peptide evidenced a significant degree of identity (62 ~ 78% identity) with other lepidopteran cecropins. Like many insect cecropin, Ay-CecA also harbored a glycine residue for C-terminal amidation at the C-end, which suggests potential amidation. To understand this peptide better, we successfully expressed bioactive recombinant Ay-CecA in Escherichia coli that are highly sensitive to the mature peptide. For this, we fused mature Ay-CecA gene with insoluble protein ketosteroid isomerase (KSI) gene to avoid the cell death during induction. The fusion KSI-CecA protein was expressed as inclusion body. The expressed fusion protein was purified by Ni-NTA immobilized metal affinity chromatography (IMAC), and cleaved by cyanogen bromide (CNBr) to release recombinant Ay-CecA. The purified recombinant Ay-CecA showed considerably antibacterial activity against Gram-negative bacteria, E. cori ML 35, Klebsiella pneumonia and Pseudomonas aeruginosa. Our results proved that this peptide with a potent antibacterial activity may play a role in the immune response of Japanese oak silkworm.

• Journal of the Mineralogical Society of Korea
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• v.16 no.2
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• pp.135-149
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• 2003

Domestic zeolite ores are mostly composed of Ca-type clinoptilolite, accompanying a little amounts of mordenite. However, other types of zeolite ores rich in ferrierite, heulandite, or mordenite are less commonly found. Based on the quantitative XRD analysis, zeolite contents are determined to be nearly 50∼90 wt%. Impurities (mostly ＞ 10 wt%) in the zeolite ores chiefly consist of quartz, feldspar, smectite, and opal-CT. The determined CEC values ($CEC_{AA}$ ) of powdery samples (grain size: < 125 $\mu\textrm{m}$) of zeolite ores by the Ammonium Acetate method are mostly higher than 100 meq/100 g. Some zeolites from the Guryongpo area, corresponding to the clinoptilolite ore, are measured to be dominantly high in CEC values ranging 170∼190 meq/100 g. Cation exchange property of the zeolite ores varies greatly depending on the types or zeolite species present in the ores. Despite of the lower grade in zeolite content, the $CEC_{AA}$ of ferrierite ore is comparatively high. Compared to this, the $CEC_{AA }$ of heulandite ore is very low, though the zeolite ore exhibits the highest grade ranging up to about 90 wt%. In addition, the CEC values calculated theoretically from the framework composition of clinoptilolite-heulandite series are not consistent with those determined by the cation exchage experiment. The measured $CEC_{AA}$ of clinoptilolite ores are generally higher than those of heulandite ores. This may be due to the higher Ca abundance in exchangeable cation composition and the presence of probable stacking faults in heulandite. The variation of $CEC_{CEC}$ is roughly proportional, though not strictly compatible, to the zeolite contents in clinoptilolite ores. It seems to be caused by the fact that the $CEC_{AA}$ of clinoptilolite locally varies depending on crystal-chemical diversity, i. e., the variation in framework composition (Si/Al) and exchangeable cation composition (especially, the contents of Ca and K). In addition, the determined CEC values ($CEC_{MB}$ ) of zeolite ores by the Methylene Blue method are much higher than those calculated from smectite contents. It suggests a probable reaction of Methylene Blue ion ($C_{16}$ $H_{18}$ $N_3$S+) with larger-pore zeolites than clinoptlolite-heulandite series, i.e., ferrierite and mordenite as well as with smectite. This can be supported by the fact that the ferrierite ore accompanying little amount of smectite has the highest value in CE $C_{MB}$ .

• Proceedings of the IEEK Conference
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• 2008.06a
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• pp.735-736
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• 2008

Recently Multi media industry require HDMI-CEC which is the system to integrate various product one action. So you need to implement CEC when you use HDMI1.3a. In this paper, presented how to make CEC protocol in MCU and how to reduce power consumption. This system implement CEC protocol and uart communication as using WELTREND MCU.

• Korean Journal of Soil Science and Fertilizer
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• v.17 no.2
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• pp.114-124
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• 1984

Various methods for measuring cation exchange capacity (CEC) of soil were compared and the contributions of ionic strength, pH and replacing cations to the CEC were investigated on Kangweon soils (Pyeongchang soils derived from lime stone : Chuncheon, Weonseong soils from alluvium : Cheolweon soils from basalt). The results were as follows : 1. The CEC measuring method using shaker and centrifuge at saturating, washing and replacing precesses, which are common in determining CEC of soils, appeared to be superior to the other methods using column, filter, or Brown method. 2. For all soil samples, the higher the ionic strength, the higher CEC value was obtained with the fewer saturating processes. However, using monovalent saturating ion on Anmi series soil derived from lime stone, the CEC value decreased when the ionic strength and the number of saturating process increased. 3. The CEC value generally increased with increasing pH. But, Chuncheon soil (Gyuam series from alluvium) having higher Al content showed the abrupt increases of CEC from pH 5.5 to pH 7.5. 4. About 70% of CEC of Kangweon soils were attributed to organic matter. 5. In determining CEC of soils, saturating with 0.5M divalent cation solution 2 to 3 times for Pyeongchang and Weonseong soil, 3 to 4 times for Cheolweon soil, and replacing with 0.25M divalent cation solution about 3 times are thought to be recommendable.

• Journal of information and communication convergence engineering
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• v.5 no.2
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• pp.155-158
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• 2007

An area distribution of Corneal Endothelial Cell(CEC) include important clinical information. In this paper, we present a two-step processing method of contour complement for the CEC. In the first step; we apply not only conventional Laplasian Gaussian filters(LGF) but also three-arrow-shaped LGFs which is newly developed to extract vertices of hexagonal shapes. In the second step; we complement the lacking part of CEC by using an energy minimum algorithm. Using the results, we measure areas of CEC.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.2
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• pp.278-283
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• 2014

Cecropins (Cec) are antibacterial peptides and their expression is induced in a pig intestinal parasite Ascaris suum by bacterial infection. To explore the usefulness of its activity as an antibiotic, CecP4 cDNA was prepared and cloned into the pPICZ B expression vector and followed by the integration into AOX1 locus in Pichia pastoris. The supernatants from cell culture were collected after methanol induction and concentrated for the test of antimicrobial activity. The recombinant P. patoris having CecP4 showed antimicrobial activity when tested against Staphyllococcus aureus in disc diffusion assay. We selected one of the CecP4 clones (CecP4-2) and performed further studies with it. The growth of recombinant P. pastoris was optimized using various concentration of methanol, and it was found that 2% methanol in the culture induced more antibacterial activity, compared to 1% methanol. We extended the test of antimicrobial activity by applying the concentrated supernatant of CecP4 culture to Pseudomonas aeruginosa and E. coli respectively. Recombinant CecP4 also showed antimicrobial activity against both Pseudomona and E. coli, suggesting the broad spectrum of its antimicrobial activity. After improvements for the scale-up, it will be feasible to use recombinant CecP4 for supplementation to the feed to control microbial infections in young animals, such as piglets.

• Proceedings of the Korean Information Science Society Conference
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• 2007.10d
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• pp.613-618
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• 2007

현재 하나의 TV에 연결할 수 있는 수많은 기기들이 개발, 판매되고 있다. 이렇게 연결하는 방법 중 현재 많이 사용하고 있는 방법 중에 하나인 HDMI의 경우에 HDMI-CEC라는 기능을 도입해서 연결된 각각의 기기를 따로따로 컨트를 하지 않고 하나의 리모컨으로 적용하는 방안을 제시하고 있다. 현재 가정에서 사용하는 DVD-Recorder에 HDMI-CEC 기능을 대응하여, 사용자가 원하는 기능을 여러 개의 기기를 직접 제어하는 것이 아니라, 단 하나의 키 동작으로 여러 개의 기기를 동시에 제어하는 시스템을 설계한다면 사용자에게 많은 편의를 줄 수가 있다. 그러나, 이러한 시스템을 설계했을 경우에 사용자가 원하는 동작을 정확히 안 하는 문제, 반응 속도가 너무 느려서 사용자가 동작을 안 하는 것으로 착각하게 만드는 문제와 HDMI-CEC의 Spec에서는 제시하고 있지 않으나 사용자의 편의물 위해서 지원해야 하는 경우가 있을 수 있다. 이 논문에서는 HDMI-CEC와 PDC의 spec에 대한 설명을 하고, HDMI-CEC를 적용한 시스템에 대한 설계를 한다. 그리고, 그 시스템에서 발견된 문제 중 정확한 동작을 하지 않는 TV가 꺼져있는 OnTouchPlay의 경우의 대한 처리와, 반응 속도가 느린 경우인 시스템을 부팅하는 경우의 반응시간을 줄이는 방안, 그리고 HDMI-CEC의 spec에는 없으나 사용자의 편의를 위해서 지원해야 하는 기능들에 대해 논한다.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.42 no.1
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• pp.95-105
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• 2019

The Cooperative Engagement Capability (CEC) System produces a synergy between the sensors and shooters that are used on various platforms by integrating them. Even the US Navy has been recently adopting the CEC system that maximizes the effectiveness of the air defense operations by efficiently coordinating the dispersed air defense assets. The Navy of other countries are conducting research studies on the theory and application methods for the CEC system. The ROK Navy has limited air defense capabilities due to its independent weapons systems on battle ships. Therefore, the ROK Navy is currently going through a phase where research on proving the validity of building the CEC system because it will provide a way to overcome the limit of the platform based air defense capability. In this study, our goal is to propose methods that maximize the air defense capability of ROK Navy, identify the available assets for constructing the CEC system, and estimate effects of the CEC system when it is applied to the naval operations. In addition, we will provide a simple model that was developed to estimate these effects and a case study with virtual data to demonstrate the effects of the system when it is applied to the naval operations. The research result of this study will provide a way for building the basis of the Korean CEC system.

• Korean Journal of Soil Science and Fertilizer
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• v.50 no.6
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• pp.653-662
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• 2017

Copper(II) acetate spectrophotometry method (CASM) was used for the rapid and convenient determination of cation exchange capacity (CEC) in soils. This method is composed of a single-step exchange reaction that adsorbs copper and is measured through spectrophotometry. The CEC of 16 Korean soils were measured using 1M ammonium acetate method (AAM) and the CASM. The CEC values determined by CASM and AAM were not significantly different, and were highly correlated ($r=0.966^{**}$). Due to the convenience, cost effectiveness, and time saving analysis of CASM, this method is recommended for most soil laboratories to measure CEC in Korean soils. However, CASM may not be applicable for soils that have a much higher CEC (greater than $20cmol_c\;kg^{-1}$).

• Korean Journal of Soil Science and Fertilizer
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• v.26 no.2
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• pp.67-71
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• 1993

This study was carried out to develop the soil conditioner and/or the absorbent of high CEC with the natural zeolite poeder whose price was very low. The $SiO_2/Al_2O_3$ ratio & CEC of the natural zeolite were 6.78 and 67.5me/100g respectively. The CEC of the post-reaction product which was treated with 3N-NaOH and $Na_2O/Al_2O_3$ ratio. 9.5 for 8hours was about 200me/100g, which was the highest value than any other treatments. The dominant clay minerals were clinoptilolite, mordenite and smectite in natural zeolite, while phillipsite in the post-reaction product.