• Title/Summary/Keyword: CD80

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Physicochemical Characterization of Chlorosome Isolated from Chlorobium limicola f. thiosulfatophilum NCIB 8327 (Chlorobium limicola f. thiosulfatophilum NCIB 8327에서 분리한 Chlorosomes의 물리화학적 특성)

  • Na, Jong-Uk;Yoon, Hwan;Kang, Sa-Ouk
    • Korean Journal of Microbiology
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    • v.31 no.1
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    • pp.9-16
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    • 1993
  • Physicochemical characteristics of chlorosomes isolated from Chlorobium lirnicoh f.thiosulfirtc~pl~ilut~i NClB 8327 were analyzed by means of UV-Visible spectrophotometer and CD-spectrophotometer. The density of the isolated chlorosomes were estimated to be 1.05 (g/$cm^{3}$) by Percoll self gradient ultracentrifugation. Chlorosome consist of bacteriochlorophyll d and some chlorobactene, and little amounl of bacteriochlorophyll a. Chlorosome is stable from 0 to $80^{\circ}C$and alkaline solution (above pH 7.0). but unstable in illuminated condition. From these results. it is suggested that some proteins or lipids may be essential for the stabilization of chlorosomes in vivo.

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A 3 Stage MMIC Low Noise Amplifier for the Ka Band Satellite Communications and BWLL System (Ka 대역 위성통신 및 BWLL 시스템용 3단 MMIC 저잡음 증폭기 설계 및 제작)

  • 염인복;정진철;이성팔
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.12 no.1
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    • pp.71-76
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    • 2001
  • A Ka Band 3-stage MMIC (Monolithic Microwave Integrated Circuits) LNA (Low Noise Amplifiers) has been designed and fabricated far the Ka band satellite communications and BWLL(Broad Band Wireless Local Loop)system. The MMIC LNA consists of two single-ended type amplification stages and one balanced type amplification stage to satisfy noise figure, high gain and amplitude linearity. The 0.15${\mu}{\textrm}{m}$ pHEMT has been used to provide a ultra low noise figure and high gain amplification. Series and Shunt feedback circuits and λ/4 short lines were inserted to ensure high stability over the frequency range form DC to 80 GHz. The size of the MMIC LNA is 3.1mm$\times$2.4mm(7.44mm$^2$). The on wafer measured performance of the MMIC LNA, which agreed with the designed performance, showed the noise figure of less than 2.0 dB, and the gain of more than 26 dB, over frequency ranges from 22 GHz to 30 GHz.

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The State of Marine Pollution in the Waters adjacent to Shipyards in Korea - 3. Evaluation of the Pollution of Heavy Metals in Offshore Surface Sediments around Major Shipyards in Summer 2010

  • Kim, Kwang-Soo
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.21 no.3
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    • pp.223-233
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    • 2015
  • In order to evaluate the pollution of heavy metals in offshore surface sediments around shipyards in Korea, surface sediment samples were collected at eleven stations around four major shipyards located in the southeastern coast of Korea in summer 2010 and nine kinds of heavy metals such as copper(Cu), zinc(Zn), cadmium(Cd), lead(Pb), chrome(Cr), arsenic(As), mercury(Hg), iron(Fe) and aluminum(Al) in sediments were analyzed. The concentrations of Cu at all sampling stations were in the range of 47.10~414.96 mg/kg and exceeded TEL(Threshold Effects Level) 20.6 mg-Cu/kg of Korean marine environmental standards for offshore sediments and ERL(Effect Range-Low) 34.0 mg-Cu/kg. The concentrations of Cu at seven stations around four shipyards were 65.18~414.96 mg/kg and exceeded PEL(Probable Effects Level) 64.4 mg-Cu/kg of Korean marine environmental standards for offshore sediments. The concentration of Cu at one station around B-shipyard was 414.96 mg/kg and exceeded ERM(Effect Range-Median) 270.0 mg-Cu/kg. The concentrations of Zn at all stations were in the range of 135.09~388.79 mg/kg which exceeded ERL 150.0 mg-Zn/kg. The concentrations of Zn at seven stations around four shipyards were 157.57~388.79 mg/kg and exceeded PEL 157.0 mg-Zn/kg. The concentration of Zn at one station around B-shipyard was 388.79 mg/kg and was approaching ERM 410.0 mg-Zn/kg. The concentrations of Cd at all stations were in the range of 0.11~0.54 mg/kg and were below TEL 0.75 mg-Cd/kg and ERL 1.2 mg-Cd/kg. The concentrations of Pb at all stations were in the range of 18.04~105.62 mg/kg. The concentrations of Pb at two stations around B-shipyard were 73.87~105.62 mg/kg which exceeded TEL 44.0 mg-Pb/kg and ERL 46.7 mg-Pb/kg, and were below PEL 119.0 mg-Pb/kg and ERM 218.0 mg-Pb/kg. The concentrations of Cr at all stations were in the range of 51.26~85.39 mg/kg. The concentration of Cr at one station around B-shipyard was 85.39 mg/kg and exceeded ERL 81.0 mg-Cr/kg. The concentrations of As at all stations were in the range of 8.70~22.15 mg/kg which exceeded ERL 8.2 mg-As/kg and were below ERM 70.0 mg-As/kg. The concentrations of As at eight stations around A-shipyard, B-shipyard and D-shipyard were 14.93~22.15 mg/kg which exceeded TEL 14.5 mg-As/kg and were below PEL 75.5 mg-As/kg. The concentrations of Hg at all stations were in the range of 0.02~0.35 mg/kg. The concentrations of Hg at three stations around A-shipyard were 0.11~0.13 mg/kg which were almost equal to TEL 0.11 mg-Hg/kg. Those at two stations around B-shipyard were 0.27~0.35 mg/kg which exceeded TEL 0.11 mg-Hg/kg and ERL 0.15 mg-Hg/kg, and were below PEL 0.62 mg-Hg/kg and ERM 0.71 mg-Hg/kg. The concentrations of Fe and Al at all stations were in the range of 2.90 3.66 % and 3.12 6.80 %, respectively. These results imply that heavy metals such as copper, zinc, lead, arsenic and mercury were likely to be transferred to marine environment from shipyards, especially from B-shipyard.

Synthesis of Na-A Type Zeolite and Its Ability to Adsorb Heavy Metals (Na-A형 제올라이트의 합성 및 중금속에 대한 흡착능)

  • Chae, Soo-Chun;Jang, Young-Nam;Bae, In-Kook;Lee, Sung-Ki;Ryou, Kyung-Won
    • Journal of the Mineralogical Society of Korea
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    • v.21 no.1
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    • pp.37-44
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    • 2008
  • This study was performed to synthesize Na-A type zeolite with melting slag from the Mapo incineration site and recycle the zeolite as an environmental remediation agent. The melting slag used had a favorable composition containing 26.6% $SiO_2$, 10.9% $Al_2O_3$ and 2.7% $Na_2O$ for zeolite synthesis although there were high contents of iron oxides, including 19.6% $Fe_2O_3$ and 18.9% FeO, which had been used as a flux for the melting. It was confirmed that the Na-A type zeolite could be successfully synthesized at $80^{\circ}C$ and $SiO_2/Al_2O_3\;=\;0.80{\sim}1.96$. The cation exchange capacities (CEC) of the zeolites was determined to be about 220 cmol/kg leveled off at the synthetic time more than 10hrs. The adsorption capacities of zeolite to heavy metals (Cd, Cu, Mn and Pb) were high except for As arid Cr. It was also confirmed through the Eh and pH analysis that As and Cr existed in the forms of $HAsO_4^{2-}$ and $CrO_4^{2-}$. The low absorption rates of zeolite for As and Cr are attributed to the fact that the pore size ($4\;{\AA}$) of Na-A type is smaller than those of $HAsO_4^{2-}$ and $CrO_4^{2-}$ ions ($4\;{\AA}$ ionic radii and $8\;{\AA}$ diameter).

Generation and characterization of 1H8 monoclonal antibody against human bone marrow stromal cells

  • Kang, Hyung Sik;Choi, Inpyo
    • IMMUNE NETWORK
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    • v.1 no.1
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    • pp.14-25
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    • 2001
  • Background: Bone marrow stromal cells (BMSCs) express many cell surface molecules, which regulate the proliferation and differentiation of immune cells within the bone marrow. Methods: To identify cell surface molecules, which can regulate cell proliferation through cell interaction, monoclonal antibodies (MoAbs) against BMSCs were produced. Among them, 1H8 MoAb, which recognized distinctly an 80 kDa protein, abolished myeloma cell proliferation that was induced by co-culturing with BMSCs. Results: IL-6 gene expression was increased when myeloma or stromal cells were treated with 1H8 MoAb. In addition, the expression of IL-6 receptor and CD40 was up-regulated by 1H8 treatment, suggesting that the molecule recognized by 1H8 MoAb is involved in cell proliferation by modulating the expression of cell growth-related genes. Myeloma cells contain high levels of reactive oxygen species (ROS), which are related to gene expression and tumorigenesis. Treatment with 1H8 decreased the intracellular ROS level and increased PAG antioxidant gene concomitantly. Finally, 1H8 induced the tyrosine phosphorylation of several proteins in U266. Conclusion: Taken together, 1H8 MoAb recognized the cell surface molecule and triggered the intracellular signals, which led to modulate gene expression and cell proliferation.

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Implementation of MDCT core in Digital-Audio with Micro-program type vector processor

  • Ku Dae Sung;Choi Hyun Yong;Ra Kyung Tae;Hwang Jung Yeun;Kim Jong Bin
    • Proceedings of the IEEK Conference
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    • 2004.08c
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    • pp.477-481
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    • 2004
  • High Quality CD, OAT audio requires that large amount of data. Currently, multi channel preference has been rapidly propagated among latest users. The MPEG(Moving Picture Expert Group) is provides data compression technology of sound and image system. The MPEG standard provides multi channel and 5.1 sounds, using the same audio algorithm as MPEG-l. And MPEG-2 audio is forward and backward compatible. The MDCT (Modified Discrete Cosine Transform) is a linear orthogonal lapped transform based on the idea of TDAC(Time Domain Aliasing Cancellation). In this paper, we proposed the micro-program type vector processor architecture a benefit in MDCT/IMDCT of MPEG-II AAC. And it's reduced operating coefficient by overlapped area to bind. To compare original algorithm with optimized algorithm that cosine coefficient reduced $0.5\%$multiply operating $0.098\%$ and add operating 80.58\%$. Algorithm test is used C-language then we designed hardware architecture of micro-programmed method that applied to optimized algorithm. This processor is 20MHz operation 5V.

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대기압 저온 아르곤 플라즈마 제트의 분광 분석

  • Han, Guk-Hui;Kim, Yun-Jung;Kim, Jung-Gil;Kim, Yeon-Jeong;Jo, Hyeon;Jo, Gwang-Seop
    • Proceedings of the Korean Vacuum Society Conference
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    • 2016.02a
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    • pp.191-191
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    • 2016
  • 대기압 저온 Ar 플라즈마 제트에서 발생되는 플라즈마에 대해 연구하였다. 플라즈마 제트의 본체는 주사기 바늘, 유리관 그리고 테프론 튜브로 구성되어 있다. 바늘의 앞부분은 유리관에 삽입되어 있으며 바늘의 뒷부분은 테프론 튜브와 연결되어 있다. 주사기 바늘에는 수십 kHz의 사인파를 발생시키는 DC-AC 인버터로 수 kV의 고전압을 인가해준다. 기체는 테프론 튜브를 통해 바늘의 안쪽으로 흐른다. 사용 기체는 Ar이며 유량은 3 lpm이다. 주사기 바늘형 전극의 내경은 1.3 mm, 외경은 1.8 mm, 총 길이는 39.0 mm이며 재질은 스테인레스강이다. 유리관의 내경은 2.0 mm, 외경은 2.4 mm, 총 길이는 80.0 mm이다. 자외선-근적외선 분광계를 이용하여 대기압 저온 Ar 플라즈마 제트에서 발생된 플라즈마의 분광 분석을 하였다. 플라즈마 제트에서 발생되는 플라즈마의 휘도는 대략 $10{\sim}30cd/m^2$이다. 플라즈마의 측정 위치, 플라즈마 제트의 입력 전압과 입력 전류, 기체 종류 등의 변수에 따른 분광 실험을 하였으며 이를 통해 얻은 분광 데이터를 일반적인 볼츠만 기울기법에 대입하여 플라즈마의 들뜸 온도를 측정하였다. 또한 Ar 플라즈마 제트의 분광 데이터를 수정된 볼츠만 기울기법에 대입하여 플라즈마의 전자 온도를 측정하였다. 이는 바이오-의료용 플라즈마 및 플라즈마 공정 등의 다양한 응용 분야에서 유용하게 활용할 수 있을 것이다.

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Estimated Toxic Metal Concentrations in Riverbank Soil of the Han and Anyang Rivers (서울한강 둔치 및 안양천 하류부 둔치주변 토양오염 조사연구)

  • Kim, Seol Ah;Lee, Jai-Young;Oh, Byung-Teak
    • Journal of Soil and Groundwater Environment
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    • v.18 no.7
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    • pp.12-17
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    • 2013
  • Heavy metal contamination of soil and water from industrial sources remains a worldwide environmental concern. Concentrations of toxic metals were measured in soil from banks of the Han and Anyang rivers. Pre-monsoon samples contained the highest heavy metal concentrations (Cu > As > Pb > Cd > $Cr^{6+}$; up to 57.80, 38.23, 25.43, 2.21, 0.32 mg/kg, respectively), but concentrations decreased at all sites during the monsoon and post-monsoon seasons. Higher heavy metal concentrations in pre-monsoon samples may be attributed to dust pollution, especially from roads near the river. A gradual reduction in heavy metal concentrations during the rainy season may be due to washing out. The high concentration of metals could cause health problems, especially in residential areas.

Immobilized Luminescent Cell - based Flow Through Monitoring of Environmental Pollutants

  • Britz, Margaret L.;Simonov, Nina;Chun, Uck-Han
    • Journal of Microbiology and Biotechnology
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    • v.7 no.4
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    • pp.250-257
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    • 1997
  • A new sensing system based on the immobilization of luminescent bacteria, photobacterium phosphoreum, was proposed for continuous real-time monitoring of pollutants. The response curves demonstrate that Photobacterium phosphoreum immobilized on the strontium alginate were very sensitive to seven reference chemicals used. The significant inhibitory concentrations for bioluminescence emission were 5 ppm for Pb$(NO_3)_2$), $NiCl_2$, $CdCl_2$, 50 ppm for $NaASO_2$, 0.1 ppm for $HgCl_2$, 0.5 ppm for pentachlorophenol and less than 5 ppm for SDS, respectively. The alginate mixed-cells (AMC) retained their luminescence during experimental period (29 days) under storage condition of $-80^{\circ}C$. The variables affecting performance of continuous flow through monitoring (CFTM) was optimized in order to ensure stability and efficiency. The flow through cell with strontium-alginate immobilized luminescent bacteria was tested with salicylate and 4-nitrophenol. A rapid response of luminescence was recorded by time drive mode in bioluminescence spectrometer after exposure to both toxicants.

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각국 대학도서관 장서구성기준의 비교고찰-기준범위의 모형정립을 위한 비교분석

  • 손정표
    • Journal of Korean Library and Information Science Society
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    • v.2
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    • pp.1-21
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    • 1975
  • Thc purposc of this .study is for ri~aking a model of building a library collcction dcnizn~cd on th.3 curriculum through thc inter-comparison of 15 standards for collcgc ant1 anivcrsily libraries in 5 countries (U. S. A.. Great Ikitain, Canada, Japan, and Icorca) Thc rcsult of thcscl colnpsrisons is as follow.. (1) Qu;~nlitalivc scope: of thc cull(:ction: (r/ Standards for a minimal collcction arc presented to about 100,000 vols (20-30 vols per student) for a ncw university and about 40,000 vols (40 vols pcr studcnt) for a nrnr collcge. $)$ Standards for a working collcction arc prescnted to 70-80 vols per student for a univer:aity and 50-60 vols per student for a college. $$ Standard for an intcnsive rescc~rch collcction is presented to morc than 100 vols p::r studcnt. (2) Stop: of the annual incrc;lsc cf thc collection: (i: Standard for a minimal collcction is ~~rescntcdto 1.4-2 vols per studcnt. $$ Standard for an intensive rrscarch or working collection is pcrsented to 4-5 vols pcr studcnt. (3) Scope of thc anni.1a1 budgetary standards: In Grcat I3ritain and U.S. A.. th-re aro assigned to 1 . 5 ~ 2 % of the collcge and university cxpcnditurr for books. $,$ In our country, it is prcscntcd to 3.5% of thc collcge and university expenditure for books, whcn my calculating by thc various factor analysis.

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