• Title/Summary/Keyword: CD18

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A Study on the developing Character Contents for the making specialized regional culture - Centering on a development project of Characters in the city of Gwangju - (지역문화 특성화를 위한 캐릭터 개발에 관한 연구 - 광주광역시 캐릭터 개발 사례를 중심으로 -)

  • 신승택;유장웅
    • Proceedings of the Korea Contents Association Conference
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    • 2004.05a
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    • pp.193-206
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    • 2004
  • 예로부터 빛고을로 인식되었던 광주가 근세에 이르러 의병활동, 광주학생운동, 5\ulcorner18민주화운동, 아직도 끝나지 않은 이념전쟁으로만 비추어질 수도 있는 지역의 이미지를 재정립하고, 도시 경쟁력의 증대를 위한 방안으로 CI사업 추진계획을 수립하고 3개년에 걸쳐(1999~2001) CI전략 개발, CI디자인 개발, 문화상품 개발, 도시환경디자인 개발 등을 추진하였다. 최근 커뮤니케이션에 있어서 캐릭터는 정보의 시각화라는 측면에서 다른 그래픽 심볼에 비해 설득적이라 할 수 있고, 기존의 심볼의 이미지 보완적 보조심볼로서의 개념이 아닌 독자적인 영역이 구축되어야 하므로 광주광역시 캐릭터 개발은 Character Identity 차원에서 메인캐릭터와 이를 각각의 용도에 맞춰 응용전개시키는 이벤트 캐릭터, 브랜드캐릭터로 구분하여 유형에 따라 다르게 적용되어야 할 적용성 및 활용성에 중점을 두고 개발되었다. 광주광역시의 메인캐릭터 개발컨셉은 "빛"과 "생명"으로 결정된 TCI이념을 활용하는 것과 광주의 상징 요소로서 "무등산"을 활용하는 두 가지 방향에서 개발이 진행되었으며, 1999년 10월의 TCI 추진회의 1차선정을 시작으로 광주시민을 포함한 전국민 설문조사와 2000년 4월의 의회설명회에 이르기까지 다양한 방법을 통해 캐릭터 개발안에 대한 평가와 검토/수정과정을 거쳐 기본캐릭터를 완성하게 되었다. 이를 통해 본 광주광역시의 캐릭터는 중장기적으로 치밀하게 계획되고 실천되어야 하며 변화하는 환경에 효율적으로 대응하는 구체적이고 실천적인 연구평가와 접근방법에 대한 연구 등으로 그 지역의 특징적인 문화를 상품화시키려는 노력이 캐릭터개발을 토대로 뒤따라야 한다. 촉진, 중요 연구 주제의 도출 및 연구 진행 계획을 수립하고, 국제적 산학 협력 강화를 통해 공동 연구를 도출하는 자리를 마련함. $\textbullet$본 학술회의에서 발표된 논문들을 취합하여, 논문집 및 CD-ROM을 제작하여 세계적 수준의 연구 결과를 알리고 이 분야에 관련된 학문, 기술 발전에 큰 도움이 됨.emove effectively radioactivity with in Adsorbent. As cleaning heavy water adsorbent and drying on each condition (temperature for drying and hours for cleaning). Because there is something to return heavy water adsorbent by removing impurities within adsorbent when it is dried o high temperature. After operating, we have been applying this research to the way to dispose heavy water adsorbent. Through this we could reduce solid waste products and the expense of permanent disposal of radioactive waste products and also we could contribute nuclear power plant run safely. According to the result we could keep the best condition of radiation s

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Production of pediocin by Chemical Synthesis and Bactericidal Mode of Action

  • Koo, Min-Seon;Kim, Wang-June;Kwon, Dea-Young;Min, Kyung-Hee
    • Proceedings of the Korean Society for Applied Microbiology Conference
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    • 2001.06a
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    • pp.149-153
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    • 2001
  • To investigate the mode of bactericidal action for antimicrobial peptide, pediocin, synthetic and mutant pediocins were prepared by direct chemical synthesis. Native pediocin was purified from Pedio-coccus acidilactici M and its conformational structure and bactericidal functions were analyzed and compared to synthetic pediocin. Schematic mode of pediocin actions, how pediocin binds on the target cell membrane, penetrates and makes tunnel are proposed. For these purposes, primary and secondary structures of pediocin was analyzed and disulfide bond assignment was also done. The pediocin purified from P. acidilactici M had high effective bactericidal ability against gram positive bacteria, especially Listeria monocytogenes and was very stable at extreme pHs and even at high temperatures such as autoclaving temperature (121$^{\circ}C$). Pediocin was consisted of 44 amino acids with four cysteines. Novel synthetic peptides were achieved by solid phase peptide synthesis(SPPS) method. To explain the function of cysteine in C-terminal region, mutant pediocin, Ped[C24A+C44A], was synthesized and their structural and biological functions were analyzed. Second mutant pediocin, Ped[KllE], was prepared to explain the function of lysine at 11 of N-terminal part of pediocin, especially loop of $\beta$-sheet, and to predict the initial binding site of pediocin. The native and synthetic pediocins was showed random coil conformation by spectropolarimetry in moderate conditions. This conformation was observed in extreme conditions such as high temperature and low and high pHs, also. Circular dichroism(CD) data also showed the existence of $\beta$-turn structure in N-terminal part both native and synthetic pediocins. A structural model for pediocin predicts that 18 amino acids in the N-terminal part of the peptide assume a three-strand $\beta$-sheet conformation. This random coil in C-terminal part of pediocin was converted to folding structure, helix structure, in nonpolar solvents such as alcohol and TFE. The disulfide bond between $^{9}$ Cys and $^{14}$ Cys was concrete and inevitable, however, evidences of disulfide bond between $^{24}$ Cys and $^{44}$ Cys was not. Data of Ped[C24A+C44A], pediocin mutant showed that $^{44}$ Cys was required during killing the target cells but not inevitable, since Ped[C24A+C44A] still have bactericidal activity but much less than native pediocin. Another pediocin mutant, Ped[KllE], had still bactericidal activity, was controversial to propose that positive charge like as $^{11}$ Lys in loop or hinge in bacteriocin bound or helped to binding to microorganism with electrostatic interaction between cell membrane especially teichoic acid and positive amino acid nonspecifically. The conformation of pediocin among native, synthetic and mutant pediocins did not show big difference. The conformations between oxidized and reduced pediocin were almost similar regardless of native or synthetic.

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Combined Trial of Fish Oil and Exercise Training Prevents Impairment in Insulin Action on Glucose Transport of Skeletal Muscle Induced by High-Fat Diet in Rats

  • Lee, Ji-Hyun;Kim, Jong-Yeon;Kim, Yong-Woon;Park, So-Young;Youn, Woon-Ki;Jang, Eung-Chan;Park, Deok-Il;Kim, Suck-Jun;Kim, Eun-Jung;Lee, Suck-Kang
    • The Korean Journal of Physiology and Pharmacology
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    • v.4 no.2
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    • pp.91-97
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    • 2000
  • The purpose of the present study was to determine the preventive effects of combined interventional trial of fish oil treatment and exercise training on insulin resistance of skeletal muscle in high-fat fed rats. Male Wistar rats were randomly divided into chow diet (CD), high-fat diet (HF), high-fat diet with fish oil (FO), high-fat diet with exercise training (EX), and FO+EX groups. The rats in control group were fed chow diet containing, as percents of calories, 58.9% carbohydrate, 12.4% fat, and 28.7% protein. High-fat diet provided 32% energy as lard, 18% as corn oil, 27% as carbohydrate and 23% as casein. The fish oil diet had the same composition as the high fat diet except that 100 g menhaden oil was substituted for corn oil. Insulin sensitivity was assessed by in vitro glucose transport in the soleus muscle after diet treatment and treadmill running for 4 weeks. While the FO or EX only partially prevented insulin resistance on glucose transport and visceral obesity induced by high-fat diet, these interventions completely corrected hyperinsulinemia and hyperglycemia from the high-fat diet. The rats in the FO+EX showed normalized insulin action on glucose transport, plasma chemicals and visceral fat mass. Insulin-mediated glucose transport was negatively associated with total visceral fat mass (r=-0.734; p<0.000), plasma triglyceride (r=-0.403; p<0.05) and lepin (r=-0.583; p<0.001) concentrations with significance. Multiple stepwise regression analysis showed that only total visceral fat mass was independently associated with insulin-mediated glucose transport (r=-0.668; p<0.000). In conclusion, combined interventional trial of FO+EX recovered insulin resistance on glucose transport of skeletal muscle induced by high-fat diet. Visceral fat mass might be more important factor than plasma TG and leptin to induce insulin resistance on glucose transport of skeletal muscle in high-fat fed rats.

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A Study of the Photoluminescence of ZnO Thin Films Deposited by Radical Beam Assisted Molecular Beam Epitaxy (라디칼 빔 보조 분자선 증착법 (Radical Beam Assisted Molecular Beam Epitaxy) 법에 의해 성장된 ZnO 박막의 발광 특성에 관한 연구)

  • Suh, Hyo-Won;Byun, Dong-jin;Choi, Won-Kook
    • Korean Journal of Materials Research
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    • v.13 no.6
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    • pp.347-351
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    • 2003
  • II-Ⅵ ZnO compound semiconductor thin films were grown on $\alpha$-Al$_2$O$_3$(0001) single crystal substrate by radical beam assisted molecular beam epitaxy and the optical properties were investigated. Zn(6N) was evaporated using Knudsen cell and O radical was assisted at the partial pressure of 1$\times$10$^{4}$ Torr and radical beam source of 250-450 W RF power. In $\theta$-2$\theta$ x-ray diffraction analysis, ZnO thin film with 500 nm thickness showed only ZnO(0002)and ZnO(0004) peaks is believed to be well grown along c-axis orientation. Photoluminescence (PL) measurement using He-Cd ($\lambda$=325 nm) laser is obtained in the temperature range of 9 K-300 K. At 9 K and 300 K, only near band edge (NBE) is observed and the FWHM's of PL peak of the ZnO deposited at 450 RF power are 45 meV and 145 meV respectively. From no observation of any weak deep level peak even at room temperature PL, the ZnO grains are regarded to contain very low defect density and impurity to cause the deep-level defects. The peak position of free exciton showed slightly red-shift as temperature was increased, and from this result the binding energy of free exciton can be experimentally determined as much as $58\pm$0.5 meV, which is very closed to that of ZnO bulk. By van der Pauw 4-point probe measurement, the grown ZnO is proved to be n-type with the electron concentration($n_{e}$ ) $1.69$\times$10^{18}$$cm^3$, mobility($\mu$) $-12.3\textrm{cm}^2$/Vㆍs, and resistivity($\rho$) 0.30 $\Omega$$\cdot$cm.

Ecological Characteristics of Actinomycetes from Mercury and Chrome Polluted Soil (수은, 6가크롬 오염토양으로부터 분리된 방선균군의 생태학적 특성)

  • Cho Min-Hye;Han Sang-Mi;Baek Ha-Ju;Whang Kyung-Sook
    • Korean Journal of Environmental Biology
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    • v.24 no.1 s.61
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    • pp.38-45
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    • 2006
  • Ecological characteristics of microbial populations inhabiting heavy metal polluted soil were investigated. The samples were collected from 293 sites around an factory and industry at Gyeoungsangbuk-do. We measured the contents of seven heavy metal elements (Cd, Cu, As, Hg, Pb, $Cr^{6+}$, CN), seven sites have been seriously contaminated by mercury and chrome. A quantitative evaluation of microbial populations in mercury and chrome contaminated soil was examined by using plate count method. Bacterial numbers in polluted soil samples ranged from $7.4X10^5\;to\;9.3X10^7\;cfu\;g^{-1}$, about $10\sim100$ fold less than the count for the unpolluted soil. Moulds were not detected in chrome polluted soil. The log values of actinomycetes of each contaminated soil samples were log ranged from 6.18 to 7.52. The ratio of actinomycetes was similar to unpolluted soil. The investigation showed actinomycetes to be the major microbial population inhabiting the mercury and chrome polluted soil. Thirty-one isolates among the total isolates were examined for antibacterial activity. These isolates were identified based on a phylogenetic analysis using 16S rRNA gene nucleotide sequences, they were categorized in three major phylogenetic groups, belong to the Streptomyces (6 strains), Saccharopolyspora (3 strains), Nocardiodes (1 strain). On the phylogenetic tree, the clade consisting of five isolates were distantly related to all of the established Streptomycetes genera, indicating the possibility as members of new species.

Assessing the Pollution Trend in Water and Sediments of Tancheon Down the Stream (탄천 상.하류의 하천수 및 퇴적물 오염도 연구)

  • Baek, Yong-Wook;Lee, Yong-Han;Kim, Hack-Kyu;Jung, Da-Un;An, Youn-Joo
    • Korean Journal of Ecology and Environment
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    • v.43 no.1
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    • pp.11-18
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    • 2010
  • The contamination of a suite of heavy metals were evaluated in water and sediments of urban stream, Tancheon down the stream. Tancheon is a urban stream which goes all the way across the urban area where various pollution sources are present. The sixteen study sites from seven different areas were designated down the stream. The heavy metal levels of the streams were detected in the order of Zn>Cu>Ni>Pb>Cd>Cr>Sb. There was a difference in the relative order of the concentration ranking in lake sediments. There is a trend that the heavy metal levels are higher at the sites where the construction causes inflow of soil particles to stream. Toxicity tests using pore-water in sediments were conducted for samples collected in some study sites, and pore-water in one site was proven to be toxic to Japanes medaka (Oryzias latipes). The reason may be the fecal pollution rather than heavy metal effect. Strong odor was detected in the sediment whose pore-water samples showed ecotoxicity. We did not observed the increasing concentration of heavy metals down to stream since the soil texture varied in the stream area of our study. Further study is needed to find quantitative relations between the level of contamination and its eco-effect.

Dependence of $O_2$ Plasma Treatment of ITO Electrode on Electrical and Optical Properties of Polymer Light Emitting Diodes (ITO 투명전극의 $O_2$ 플라즈마 처리가 고분자 유기발광다이오드의 전기.광학적 특성에 미치는 영향)

  • Gong, Su-Cheol;Back, In-Jea;Yoo, Jea-Huyk;Lim, Hun-Sung;Yang, Sin-Huyk;Shin, Sang-Bea;Shin, Ik-Seup;Chang, Gee-Keun;Chang, Ho-Jung
    • Journal of the Korean institute of surface engineering
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    • v.39 no.3
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    • pp.93-97
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    • 2006
  • Polymer light emitting diodes (PLEDs) are expected to be commercialized as next generation displays by advantages of the fast response time, low driving voltage and easy manufacturing process for large sized flexible display. Generally, the electrical and optical properties of PLEDs are affected by the surface conditions of transparent electrode. The PLED devices with ITO/PEDOT:PSS/PVK/PFO-poss/LiF/Al structures were prepared by using the spin coating method. For this, PEDOT:PSS(poly(3,4-ethylenedioxythiophene):poly(styrene sulfolnate)) Al 4083 and PVK(N-vinylcabozole) were used as hole injection and transport layers. The PFO-poss(poly(9,9-dioctylfluorene)) was used as the emitting layer. The dependence of $O_2$ plasma treatment of ITO electrode on the electrical and optical properties of PLEDs were investigated. The sheet resistances increased slightly with an improved surface roughness of ITO electrode as the RF power increased during $O_2$ plasma treatment. The PLED devices prepared on the ITO/Glass substrates, which were plasma-treated at 40 watt in RF power for 30 seconds under 40 mtorr $O_2$ pressure, showed the maximum external emission efficiency of 0.86 lm/W and the maximum luminance of $250\;cd/m^2$, respectively. The CIE color coordinates are ranged $X\;=\;0.13{\sim}0.18$ and $Y\;=\;0.10{\sim}0.16$, showing blue color. emission.

A Study of Monitoring Method on Exposure Level and Biomarkers of Environmental Pollutants -Focused on Ulsan Industrial Complex Area- (지역주민 환경오염 노출수준 및 생체지표 모니터링 방법에 대한 연구 -울산지역을 중심으로-)

  • Lee, Jong-Tae;Cho, Yong-Sung;Son, Ji-Young;Lee, Joung-Won;Lee, Seung-Jun;Chung, Young-Hee;Kim, Dae-Seon;Yu, Seung-Do;Ahn, Seung-Chul
    • Journal of Environmental Health Sciences
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    • v.34 no.3
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    • pp.188-198
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    • 2008
  • Since 2003, the National Institute of Environmental Research (NIER) of Korea has been conducting "The monitoring study on exposure level and biomarkers of environmental pollutants" in the Ulsan industrial complex with the goals to establish a surveillance system for residents, to evaluate the health effects associated with chronic exposure to environmental pollutants and to develop the environmental health indicators in Ulsan industrial complex. This program consists of three phases an initial or foundation phase in which the program is developed, made operational and extended to the community, followed by an evaluation and accountability assessment of the surveillance system and finally an improvement in the quality of life and the maintenance of good health for Ulsan residents. In the period 2003 to 2008, the study program developed the surveillance system which will be used for the full-length survey. All participants in this study were contacted at a presentation meeting for residents, introduced to the objectives and protocols of the survey, and asked to participate. Informed consent was obtained from each participant. The study is now underway and includes questionnaires, health examinations and the analysis/collection/banking of bio-sample such as blood and urine. To date 828 subjects have participated and already 588 subjects have been followed up. We are committed to complete health examinations and to arrange storage of biosample for all participants. During the current year, we will analyze metals (Pb in blood and Cd, inorganic As and Hg in urine) in 1,972 samples and VOCs (11 species) and PARs (18 species) in 300 samples (blood sample). Moreover, the summary of the first step and the further preparation of the second step are currently underway. In this article, we introduce the study and its protocols and the distribution and size of the study participants. In conclusion, this survey will be continuously conducted on the established cohort and will enable the identification of relationship between environmental pollutant exposures and the health status of residents in Ulsan industrial complex.

Nutritional Adequacy Analysis of Recommended Menu in Dietary Reference Intakes for Koreans 2015 (식사구성안의 생애주기별 권장 식단의 영양 적정성 분석 -2015 한국인 영양소 섭취기준-)

  • Kim, Youngnam
    • Korean Journal of Community Nutrition
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    • v.22 no.4
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    • pp.279-288
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    • 2017
  • Objectives: Recommended Menu (RM) prepared according to the Target Pattern expected to meet the dietary reference intake (DRI) of nutrients. Nutritional adequacy of RM in 'DRI for Koreans 2015' were analyzed to verify whether such expectation was fulfilled. Methods: Dishes in RM are categorized by 5 food groups, and number and types of dishes for main meal and between-meals were analyzed. The energy and 12 nutrients (protein, dietary fiber, Ca, P, Fe, Na, K, vitamin A, thiamin, riboflavin, niacin and vitamin C) contents in 10 RMs were calculated using the food composition table (CD) in 'DRI for Koreans'. Energy, energy contribution ratio, and nutrient contents in 10 RMs for 18 age groups were evaluated based on the 'DRI for Koreans 2015'. Results: Number of dishes per meal were 4.83, and representative table setting consisted of cooked rice + soup (or stew) +3 side dishes. Energy contents of RM were 75~109% of estimated energy requirement (EER). None of the RM met the DRI of all 12 nutrients examined. Calcium was the most insufficient nutrient. Only 1-2 years' RM met the DRI, all the other RMs did not meet the calcium DRI. Dietary fiber and potassium contents were also insufficient in most RM. In adult male's RM, only 1 nutrient, i.e. calcium did not meet the DRI, but in female adult's RM, 5~6 nutrients did not meet the DRI. Energy contribution ratio of carbohydrate, protein, and fat in RM were 59.0~70.4%, 15.7~17.5%, 12.1~23.5%, respectively. And 4 RMs out of 10 exceeded the upper limit of acceptable macro-nutrient distribution range (AMDR) of carbohydrate and 3 RMs out of 10 RM were below the lower limit of fat AMDR. Contribution ratio of nutrients were ${\geq}40%$ by food groups were as follows: grain group in energy and carbohydrate; meat fish egg legume group in fat, protein, and niacin; vegetables group in dietary fiber, vitamin A, and vitamin C; milk dairy products group in calcium. Conclusions: RM prepared according to the Target Pattern did not meet nutritional adequacy as expected. Especially calcium, potassium, and dietary fiber contents are needed to be increased in many RMs. Further, energy content in RM needs to be adjusted for fat sugar food group assigned in Target Pattern and condiment.

Characteristics of Water Quality in Upper Stream Watershed of Dongjin River (동진강 상류하천 유역의 수질특성)

  • Son, Jae-Gwon;Park, Jong-Min;Choi, Jin-Kyu;Song, Jae-Do
    • Journal of Korean Society of Rural Planning
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    • v.9 no.2 s.19
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    • pp.19-28
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    • 2003
  • It is the purpose of this study to investigate the change of stream water quality in upper stream of Dongjin river, and to give the basic information for the conservation of water quality. Water samples were taken periodically at 9 sampling sites during 8 months from March to October in 2002. The results of this study were as follows : 1. The water temperature and pH of stream water were ranged $9.0{\sim}29.4^{\circ}C,\;6.48{\sim}9.33$, respectively. The COD values of stream water was ranged from 0.60 to 19.06. The contents of T-N and T-P mainly affected by the livestock wastes, and agricultural activity were $1.88{\sim}6.74\;mg/L$, ND(not deleted)${\sim}0.50\;mg/L$, respectively. 2. The SS, DO and BOD values of stream water were ranged $0.4mg/L{\sim}274.0mg/L$, $0.5{\sim}6.0\;mg/L$, and $7.3{\sim}13.7\;mg/L$ respectively. 3. The cation is one of the important components in analysis of stream water quality. The contents of analysis, $Ca^{++},\;Mg^{++},\;Na^+$ and $K^+$ were ranged $1.96{\sim}11.08\;mg/L$, $1.21{\sim}6.16\;mg/L$, $3.38{\sim}18.44\;mg/L$, $1.12{\sim}7.96\;mg/L$, respectively. SAR was ranged $0.31{\sim}1.63$ below 2.0. The contents of cation showed in the order $Na^{++}>Ca^{++}>K^+>Mg^+$. 4. The contents of heavy metal Zn, Cu, and Pb were $ND{\sim}0.071\;mg/L$, $ND{\sim}0.012\;mg/L$, and $ND{\sim}0.043\;mg/L$, respectively. Cd was not detected in all samples. 5. As a result of these researches showed water quality in upper stream watershed of Dongjin river more affected by livestock wastes and living sewage than agricultural activity.