• 한국HCI학회:학술대회논문집
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• 한국HCI학회 2009년도 학술대회
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• pp.818-825
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• 2009

에듀테인먼트는 유아용 인터렉티브 TV 를 연구하는데 있어서 가장 중요한 요소 중 하나이며, 유아교육시장에서의 이와 관련된 연구는 시장성으로나 소비자 수요 측면에서 큰 비중을 차지하고 있다. 이 연구의 목적은 아이들의 생활, 행태를 관찰, 조사 분석하여 발견된 중요한 요소들이 가지는 교육적 효과를 디자인을 통해 적용하여 에듀테인먼트 효과가 최대화된 유아용 인터렉티브 TV 를 위한 새로운 아이디어를 제시할 수 있도록 하고자 함이다. 아이들의 노는 모습-책 읽기, 음악듣기, 장난감가지고 놀기 등-아이들이 접하는 미디어들의 관찰을 통해 교육적 목적으로 환영 받지 못하는 TV 를 신뢰할 수 있는 매체로 재해석 하기 위하여 새로운 개념의 단서를 찾고자 하였다. 새로운 서비스를 제안하기 위하여 사용자가 기존의 미디어를 통해 상호작용하는 행태를 관찰, 분석하여 그 결과를 유아용 인터렉티브 TV 의 서비스를 위한 사용자 경험 정보 구조와 그 경험을 가능하게 하는 Input 요소를 통한 인터렉션 방법의 제안을 함께 하였다. 본 연구에서 제시된 콘텐트의 경험구조와 그와 연동 가능한 input device 를 통해 좀 더 창의적인 상호작용이 가능한 유아용 인터렉티브 TV 를 위한 콘텐트 디자인이 가능할 것으로 생각된다. 초기의 TV 에서 IPTV 까지 TV 는 변천을 거듭하여 왔고 네트워크라는 인프라의 중심에 서게 되면서 인터렉션 요소를 통해 에듀테인먼트 효과가 극대화 될 수 있는 매체로써 새로운 전기를 맞이하게 될 것으로 예상된다.

• 한국비블리아학회지
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• 제28권3호
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• pp.217-240
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• 2017

이 연구는 영상콘텐츠분야 연구의 의제설정 경향을 분석하여, 정권별 정책과 연결되는 의미화 과정을 제시했다. 이를 위해 문화산업의 도입시점인 <문민정부(1993년)>부터 <박근혜정부(2016년)>까지의 영상콘텐츠 석박사학위논문 총 2,624편의 초록에서 43,991개의 단어를 추출하고 의미연결망 분석을 실시하였다. 분석방법은 R프로그램의 다양한 패키지를 활용하였으며, 이를 통해, 텍스트 분석과 시각화를 도출하였다. 연구 결과는, 첫째, 영상콘텐츠분야 연구는 출현빈도별, 정권순서별로 '영상', '미디어', '콘텐츠'의 순위와 순서로 진화되었다. 둘째, 정권별로는 3단계 연구흐름을 볼 수 있다. <문민정부>는 '교육'과 '표현', <국민의정부>와 <참여정부>에서는 '미디어', <이명박정부>와 <박근혜정부>에서는 '콘텐츠'관련 연구들이 중심이 되었다. 셋째, 연구대상 기간 또는 정권별 기간 내내 지속적으로 꾸준하게 진행되는 연구주제는 '방송', '디지털', '기술', '제작' 등이며, 향후에도 계속 진행될 것으로 보인다. 마지막으로 각 정권마다 새롭게 등장한 연구대상이 있었다. <문민정부>는 '콤팩트디스크기억장치(CD-ROM)', <국민의정부>는 '워터마크', '고화질', '3D', '가상현실', <참여정부>는 '플랫폼', <이명박정부>는 '모바일', '애플리케이션', <박근혜정부>는 '스마트'이며, '콤팩트디스크기억장치'와 '워터마크' 등은 단기에 소멸되었다. 연구의 의제설정과 산업화 과정에서 트렌드와 미래예측이 필요하다고 보여 진다.

• Transactions on Electrical and Electronic Materials
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• 제8권6호
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• pp.260-264
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• 2007

We have studied stability in organic light-emitting diode depending on buffer layer and cathode. A transparent electrode of indium-tin-oxide(ITO) was used as an anode. An electron injection energy barrier into organic material is different depending on a work function of cathodes. Theoretically, the energy barriers for the electron injection are 1.2 eV, -0.1 eV, and 0.0 eV for Al, LiAl, and LiF/Al at 300 K, respectively. We considered the cases that holes are injected to organic light-emitting diode. The hole injection energy barrier is about 0.7 eV between ITO and TPD without buffer layer. For hole-injection buffer layers of CuPc and PEDOT:PSS, the hole injection energy barriers are 0.4 eV and 0.5 eV, respectively. When the buffer layer of CuPc and PEDOT:PSS is existed, we observed the effects of hole injection energy barrier, and a reduction of operating-voltage. However, in case of PVK buffer layer, the hole injection energy barrier becomes high(1.0 eV). Even though the operating voltage becomes high, the efficiency is improved. A device structure for optimal lifetime condition is ITO/PEDOT:PSS/TPD/$Alq_3$/LiAl at an initial luminance of $300cd/m^2$.

• Journal of Microbiology and Biotechnology
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• 제8권2호
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• pp.152-157
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• 1998

To analyze the role of the C and D domains in the cyclization activity of cyclodextrin glucanotransferase (CGTase), two plasmids, pKB1ΔC300 and pKB1ΔD96, were constructed in which DNA regions encoding 100 and 32 amino acids, respectively, from the C and D domains of B. stearothermophilus NO2 CGTase were deleted. The mutated CGTase from the pKBlΔC300 produced much lower amounts of ${\alpha}$-, ${\beta}$-, and $\gamma$-cyclodextrin (CD) than the parental CGTase. However, the mutated CGTase from the pKBlΔD96 showed a similar production pattern of CDs to wild-type CGTase. The production ratios of the ${\alpha}$-, ${\beta}$- and $\gamma$-CDs were not affected by the deletions, when compared to those of parental CGTase. The optimum temperature of the mutated CGTase from the pKBlΔC300 was decreased from $60^{\circ}C$ to $55^{\circ}C$. The optimum pH of the mutated CGTase from the pKB1D96 was shifted from 6.0 to 7.0. The thermostability of the two mutant CGTases were not changed. From these results, it is suggested that the C and D domains are not related to cyclization activity directly because mutant-enzymes deleted C or D domains still possessed their activity. However, they are important for other enzymatic properties such as productivity and pH optimum as a partition of CGTase tertiary structure.

• Environmental Engineering Research
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• 제10권5호
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• pp.205-212
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• 2005

The study was conducted to investigate the removal of heavy metals by using Hydroxyapatite(HAp) made from waste oyster shells and wastewater with high concentration of phosphorus. The maximum calcium concentration for the production of HAp in this study was released up to 361 mg/L at pH of 3 by elution experiments. When the pH was at adjusted 6, the maximum calcium released concentration was 41 mg/L. During the elution experiment, most of the calcium was released within 60 minutes. This reaction occurred at both pH levels of 3 and 6. The result of the XRD analysis for the HAp product used in this study shows the main constituent was HAp, as well as OCP. The pH was 8.6. As the temperature increased, the main constituent did not vary, however its structure was crystallized. When the pH was maintained at 3, the removal efficiency decreased as the heavy metal concentration increased. The order of removal efficiency was as follows: $Fe^{2+}$(92%), $Pb^{2+}$(92%) > $Cu^{2+}$(20%) > $Cd^{2+}$(0%). Most of these products were dissolved and did not produce sludge in the course of heavy metals removal. As the heavy metal concentration increased at pH of 6, the removal efficiency increased. The removal efficiencies in all heavy metals were over 80%. From the analysis of the sludge after reaction with heavy metals, the HAp was detected and the OCP peak was not observed. Moreover, lead ion was observed at the peaks of lead-Apatite and lead oxidant. In the case of cadmium, copper and iron ions, hydroxide forms of each ion were also detected.

• 한국자기공명학회논문지
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• 제6권1호
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• pp.54-68
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• 2002

PEBP2/CBF (Polyomavirus Enhancer-core Binding Protein 2/Core Binding Factor), represents a new family of heterodimeric transcription factor. Those members play important roles in hematopoiesis and osteogenesis in mouse and human. PEBP2/CBF is a sequence-specific DNA binding protein. Each member of the PEBP2/CBF family of transcription factors is composed of two subunits, ${\alpha}$ and ${\beta}$. The evolutionarily conserved 128 amino acid region in ${\alpha}$ subunit has been called the Runt domain, which harbors two different activities, the ability to bind DNA and interact with the ${\beta}$ subunit. Recently, cDNA clones encoding the C. elegans Runt domain were isolated by screening a cDNA library. This gene was referred to run (Runt homologous gene). In this study, the basic experiments for the structural characterization of RUN protein were performed using spectroscopic methods. We have identified the structural properties of RUN using bioinformatics, CD and NMR. The limit temperature of the structural stability was up to 60$^{\circ}C$ with irreversible thermal process, and the structure of RUN seems to adopt ${\alpha}$ helices and one or more ${\beta}$ sheet or turn. The degree of NMR peak dispersion and intensity was increased by addition of glycine. Therefore, glycine could be used to alleviate the aggregation property of RUN in NMR experiment.

• Bulletin of the Korean Chemical Society
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• 제28권12호
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• pp.2303-2309
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• 2007

Expressed protein ligation (EPL) technique, joining recombinantly expressed proteins to polypeptides, has been widely adopted for addressing various biological questions and for drug discovery. However, joining two recombinant proteins together is sometimes difficult when proteins are expressed insoluble and unrefoldable, because ligation-active proteins via intein-fusion are obtainable when they are folded correctly. We overcame this limitation coexpressing target protein with additional methionine aminopeptidase (MAP) which enhances removal of the initiation methionine of recombinantly expressed protein. Our approach demonstrated that two domains of 46 kDa 5-Enolpyruvylshikimate-3-phosphate (EPSP) synthase, a target of herbicide glyphosate, were successfully joined by native chemical ligation, although its C-terminal domain was expressed as an inclusion body. The intein-fused N-terminal fragment of EPSP synthase (EPSPSN, residues 1-237) was expressed and the ligation-active thioester tagged N-terminal fragment (EPSPSN-thioester) was purified using a chitin affinity chromatography and mercapto-ethanesulphonate (MESNA) as intein thiolysis reagent. Its Cterminal fragment (EPSPSC, residues Met237-238CYS-427), expressed as an inclusion body, was prepared from an additional MAP-expressing strain. Protein ligation was initiated by mixing ~1 mM of EPSPSN-thioester with ~2 mM of EPSPSCCYS (residues 238CYS-427). Also we found that addition of 2% thiophenol increased the ligation efficiency via thiol exchange. The ligation efficiency was ~85%. The ligated full-length EPSP synthase was dissolved in 6 M GdHCl and refolded. Circular dichroism (CD) and enzyme activity assay of the purified protein showed that the ligated enzyme has distinct secondary structure and ~115% specific activity compared to those of wild-type EPSP synthase. This work demonstrates rare example of EPL between two recombinantly expressed proteins and also provides hands-on protein engineering protocol for large proteins.

• Bulletin of the Korean Chemical Society
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• 제32권9호
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• pp.3223-3228
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• 2011

To quantify the presence of mercuric ions in aqueous solution, double-stranded DNA (dsDNA) of poly(dT) was employed using a light switch compound, $Ru(phen)_2(dppz)^{2+}$ (1) which is reported to intercalate into dsDNA of a right-handed B-form. Addition of mercuric ions induced the dehybridization of poly(dT)${\cdot}$poly(dA) duplexes to form a hairpin structure of poly(dT) at room temperature and the metal-to-ligand charge transfer emission derived from the intercalation of 1 was reduced due to the dehybridization of dsDNA. As the concentration of $Hg^{2+}$ was increased, the emission of 1 progressively decreased. This label-free emission method had a detection limit of 0.2 nM. Other metal ions, such as $K^+$, $Ag^+$, $Ca^{2+}$, $Mg^{2+}$, $Zn^{2+}$, $Mn^{2+}$, $Co^{2+}$, $Ni^{2+}$, $Cu^{2+}$, $Cd^{2+}$, $Cr^{3+}$, $Fe^{3+}$, had no significant effect on reducing emission. This emission method can differentiate matched and mismatched poly(dT) sequences based on the emission intensity of dsDNA.

• Bulletin of the Korean Chemical Society
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• 제32권9호
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• pp.3327-3332
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• 2011

Antimicrobial peptides have recently gained the much attention because of their ability to make defense system from attacking bacterial infections. Drosocin has been considered as very attractive antibiotic agents because of low toxicity against human erythrocytes and active at the low concentration. We have studied the structureactivity relationship of a glycopeptide drosocin focused on the N-acetyl-D-galactoside at $Thr^{11}$ residue. Based on the radial diffusion assay, we found that the acetylation of carbohydrate moiety increased the antimicrobial activity and the $Pro^{10}$, present in the middle of drosocin plays an important role in the antimicrobial activity. Our results provide a good lead compound for further studies on the design of drosocin-based analogues targeting glyco linked Thr site.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2000년도 학술대회 논문집 전문대학교육위원
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• pp.35-39
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• 2000

$ZnIn_2S_4$ and $ZnGaInS_4:Er^{3+}$ single crystals crystallized in the rhombohedral (hexagonal) space group $C_{3v}^5(R3m)$, with lattice constants $a=3.852{\AA},\;c=37.215{\AA}$ for $ZnIn_2S_4$, and $a=3.823{\AA}$, and $c=35.975{\AA}$ for $ZnIn_2S_4:Er^{3+}$. The optical absorption measured near the fundamental band edge showed that the optical energy band structure of there compounds had a direct and indirect band gap, the direct and indirect energy gaps are found to be 2.778 and 2.682 eV for $ZnIn_2S_4$, and 2.725 and 2.651eV for $ZnIn_2S_4:Er^{3+}$ at 293 K. The photoluminescence spectra of $ZnIn_2S_4:Er^{3+}$ measured in the wavelength ranges of $500nm{\sim}900nm$ at 10 K. Eight sharp emission peaks due to $Er^{3+}$ ion are observed in the regions of $549.5{\sim}550.0nm,\;661.3{\sim}676.5nm$, and $811.1{\sim}834.1nm$, and $1528.2{\sim}1556.0nm$ in $CdGaInS_4:Er^{3+}$ single crystal. These PL peaks were attributed to the radiative transitions between the split electron energy levels of the $Er^{3+}$ ions occupied at $C_{2v}$, symmetry of the $ZnIn_2S_4$ single crystals host lattice.