• Journal of environmental and Sanitary engineering
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• v.17 no.3
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• pp.21-30
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• 2002

This study was Performed to evaluate the effective solidification of Cd sludge using cement and power plant fly ash as cement admixture, to identify the leaching characteristics of the heavy metal Cd sludge solidified, and to develope proper KLT(Korean Leaching Test) of hazardous waste. KLT was compared with EPT(Extraction Procedure Toxicity) and TCLP(Toxicity Characteristics Leaching Procedure). Fly ash contents ranged from 0% to 30% of cement weight. The experimental results showed that the optimum amount of fly ash replaced was 10% to 15% and KLT was less appropriate than EPA and TCLP. Also the purpose of the study was to suggest the modification factors on the leaching test currently used, based on the above mentioned aspects. The effects of pH, leaching time, leaching for agitating intensity, and leaching solvent were investigated. As the result of test, the leaching potential was relatively high when the pH and agitation intensity of leaching solution were 5 and 150rpm, respectively. Leaching time of six hours was found to be sufficient and the use of acetic acid as a leaching solvent is more useful in landfill site particularly.

• Archives of Pharmacal Research
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• v.27 no.6
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• pp.633-639
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• 2004

The expression of therapeutic transgenes in recombinant adenoviral vectors is a major cause of toxicity in dividing cancer cells as well as non dividing normal cells. To solve the problem of toxicity to normal cells, we have reported on a recombinant adenoviral vector system （AdLP-） in which the expression of the transgene is directed by the tumor-specific L-plastin promoter （LP） （Chung et al., 1999）. The object of this study was to generate a recombinant adenoviral vector system which would generate tumor cell specific expression of cytosine deaminase （CD） gene. We report the construction of a replication-incompetent adenoviral vector in which CD is driven by the L-plastin promoter （AdLPCD）. Infection of 293 cells by AdLPCD generated the functional CD protein as measured by HPLC analysis for the conversion of 5-Fluorocy-tosine （5-FC） to 5-Fluorouracil （5-FU）. HPLC analysis in conjunction with counting radioactivity for ［6-$^3$H］-5FC and ［6-$^3$H］-5FU demonstrated vector dose-dependent conversion of 5-FC to 5-FU in AdLPCD infected ovarian cancer cells. The results from present and previous studies（Peng et al., 2001； Akbulut et al., 2003） suggest that the use of the AdLPCD／5-FC system may be of value in the treatment of cancer including microscopic ovarian cancer in the peritoneal cavity.

• Journal of Applied Biological Chemistry
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• v.57 no.2
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• pp.131-136
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• 2014

An attempt was made to assess metal ionic toxicity levels of different fishes in the polluted rivers viz., Buriganga and Turag. Fish samples collected from two polluted rivers were analyzed for the levels of metals such as Cd, Cr, Cu, Mn, Pb, and Zn in order to elucidate the status of these contaminants in fish meant for human consumption. The detected concentrations of Cr, Cu, Mn, and Zn ions in fish species collected from the polluted rivers were below the toxic levels and did not appear to pose a threat. Among the analyzed metals, Cd and Pb ions were detected above the permissible levels in liver and muscle tissues of stinging catfish (Heteropneustesfossilis), spotted snakehead (Channapunctata) and wallago (Wallagoattu) collected from the polluted rivers causing toxicity for human consumption. Stinging catfish (Heteropneustesfossilis) was the species found to highly bioaccumulate these metals. Fish species bioconcentrated appreciable amounts of Cd and Pb as toxic metals in the liver as compared to the muscle. Levels of these toxic metals varied depending on different tissues in fish species.

• Environmental Analysis Health and Toxicology
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• v.22 no.2 s.57
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• pp.171-175
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• 2007

Indigenous species means a species that is likely, due to historical presence, to occur at a specified site for some portion of its life span. Therefore, indigenous species can be useful as an indicator to assess environmental risk caused by hazardous chemicals in a specific site. So far a few toxicity studies using freshwater species which are indigenous to Korea have been carried out. In this study, a freshwater shrimp (Neocardina denticulata) indigenous to Korea was used for acute toxicity test of heavy metals. Neocardina denticulata were exposed to cadmium chloride $(CdCl_2)$, copper chloride $(CuCl_2)$ and zinc chloride $(ZnCl_2)$ using automatic flow-through system for 96 hours. The 96h LC50s were calculated as 0.043 $(0.042{\sim}0.045)mg\;CdCl_2/L,\;0.104(0.098{\sim}0.113)mg\; CuCl_2/L\;and\;2.021\;(1.633{\sim}2.594)mg\;ZnCl_2/L$. When compaired with some international standard species such as medaka(Oryzias latipes), Neocardina denticulata had high sensitivity. Therefore, this study suggested that Neocardina denticulata have possibilities for a sensitive test species to test heavy metal toxicity in aqua-system.

• Environmental Analysis Health and Toxicology
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• v.19 no.2
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• pp.141-151
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• 2004

We exposed juvenile puffer fish, Takifugu obscurus(30 days after hatching) to various aqueous pollutants including 4 kinds of inorganic metals (Ag, Cd, Cu and Hg), 2 organotin compound.; (tributyltin ［TBT］ and triphenyltin［TPhT］) and 5 polycyclic aromatic hydrocarbon (PAH) compounds (chrysene, fluoranthene, naphthalene, phenanthrene and pyrene) to estimate median lethal concentrations (LC50s) of each pollutant after the 96-hour acute exposure. Among the inorganic metals, Hg (52 $\mu\textrm{g}$/L; 96-h LC50) was most toxic to test animals and followed by Ag (164 $\mu\textrm{g}$/L), Cu (440 $\mu\textrm{g}$/L) and Cd (1180 $\mu\textrm{g}$/L). Aqueous TBT was more toxic between the two organotins; the 96-h LC50 for TBT (5.1 $\mu\textrm{g}$/L) was 3 times lower than that of TPhT (17.3 $\mu\textrm{g}$/L). The acute toxicity of PAH compounds was highest for chrysene (1.5 $\mu\textrm{g}$/L; 96-h LC50) and decreased in the order of pyrene (65 $\mu\textrm{g}$/L) > fluoranthene (158 $\mu\textrm{g}$/L) > phenanthrene (432 $\mu\textrm{g}$/L) > naphthalene (8690 $\mu\textrm{g}$/L). The toxicity of PAH compounds wat closely related to their physico-chemical characteristics such as $K_{ow}$ and water solubility, and well explained by simple QSAR relationship. The sensitivity of puffer fish to various inorganic and organic pollutants was generally comparable to various fish species widely used as standard test species in previous studies and further evaluation should be conducted to develop adequate testing procedure for T. obscurus when used in various toxicity tests.

• Nutrition Research and Practice
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• v.5 no.6
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• pp.520-526
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• 2011

Folate deficiency and hyperhomocysteinemia are found in most patients with alcoholic liver disease. Oxidative stress is one of the most important mechanisms contributing to homocysteine (Hcy)-induced tissue injury. However it has not been examined whether exogenous administration of folic acid attenuates oxidative stress and hepatic toxicity. The aim of this study was to investigate the in vivo effect of folic acid supplementation on oxidative stress and hepatic toxicity induced by chronic ethanol consumption. Wistar rats (n = 32) were divided into four groups and fed 0%, 12%, 36% ethanol, or 36% ethanol plus folic acid (10 mg folic acid/L) diets. After 5 weeks, chronic consumption of the 36% ethanol diet significantly increased plasma alanine transaminase (ALT) (P < 0.05) and aspartate transaminase (AST) (P < 0.05), triglycerides (TG) (P < 0.05), Hcy (P < 0.001), and low density lipoprotein conjugated dienes (CD) (P < 0.05) but decreased total radical-trapping antioxidant potential (TRAP) (P < 0.001). These changes were prevented partially by folic acid supplementation. The 12% ethanol diet had no apparent effect on most parameters. Plasma Hcy concentration was well correlated with plasma ALT (r = $0.612^{**}$), AST (r = $0.652^*$), CD (r = $0.495^*$), and TRAP (r = $-0.486^*$). The results indicate that moderately elevated Hcy is associated with increased oxidative stress and liver injury in alcohol-fed rats, and suggests that folic acid supplementation appears to attenuate hepatic toxicity induced by chronic ethanol consumption possibly by decreasing oxidative stress.

• Journal of Environmental Science International
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• v.13 no.6
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• pp.513-518
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• 2004

3-weeks old Commelina was transferred to and grown in Hoagland solution (Control, $100\muM$ $Cd^{2+}$, $100\muM$ $Cd^{2+}$+ $100\muM$ IAA, $100\muM$ $Cd^{2+}$+ $100\muM$ IAA + 2 mM sucrose) for 3 weeks and then the effects of indole acetic acid (IAA) on the accumulation of $Cd^{2+}$ and growth of $Cd^{2+}$-treated Commelina were investigated. In the treatment of $Cd^{2+}$, $Cd^{2+}$ was uptaked to 1.74, and 51.36 ${\mu}g/g$ frwt. at the first week, but for three weeks, 0.51 and 34,53 ${\mu}g/g$ frwt, in leaf and stem respectively. When IAA was treated along with $Cd^{2+}$, $Cd^{2+}$ was uptaked to 0.18 and 8.63 ${\mu}g/g$ fiwt, at the first week, and for the incubation of 3 weeks, 0,51 and 45.0 ${\mu}g/g$ fiwt. in leaf and stem. In case of $Cd^{2+}$+IAA+sucrose, $Cd^{2+}$ was uptaked to 1.45 and 18.33 ${\mu}g/g$ frwt. at the first week, but for 3 weeks, 0,51 and 25.45 ${\mu}g/g$ fiwt. in leaf and stem. Likewise $Cd^{2+}$ uptake, the growth was also affected by $Cd^{2+}$ and IAA. During the incubation of 3 weeks, $Cd^{2+}$ reduced the stem growth about 8% in all weeks, but the treatment of IAA recovered the inhibition of stem growth caused by $Cd^{2+}$ to the degree of the control Therefore, it could be concluded that IAA altered the pattern of $Cd^{2+}$ uptake and the growth which were supposed to change $Cd^{2+}$ toxicity.

• Analytical Science and Technology
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• v.14 no.2
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• pp.173-179
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• 2001

The study was carried out to analyze the pollutant Fe, Cu, Cr, Zn, Cd for sediments collected from lake in K river basin. Three analytical methods currently used in Korea, Japan, U.S.A, were compared. Pretreatment using microwave digestion showed higher analytical results for Fe, Cu, Cr, Zn, Cd than Korean Official Method(KOM) and American Toxicity Characteristic Leaching Procedure(TCLP) Method. Also, analytical results using microwave digestion, TCLP and KOM were as follows: 38.1-48.0 mgFe/kg, 10.2-15.9 mgFe/kg AND 3.5-12.6 mgFe/kg, 37.0-50.1 mgCu/kg, 0.06-0.24 mgCu/kg and 0.01-0.03 mgCu/kg, 137.0-152.0 mgZn/kg, 0.67-0.82 mgZn/kg and 0.3-0.5 mgZn/kg, respectively. From this result, a new analytical method for the determination of heavy metal in sediment should be developed for the accurate estimation of pollution degree in sediment.

• Environmental Analysis Health and Toxicology
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• v.24 no.1
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• pp.9-16
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• 2009

Effects of salinity and standard toxic metals on fertilization and embryo development rates were investigated in the sea urchin, Hemicentrotus pulcherrimus. Spawning was induced by injecting $1{\sim}2$ mL of 0.5 M KCl into the coelomic cavity. Males released white or cream-colored sperms and females released yellow or orange-colored eggs. The fertilization rate was below 30% when salinity was 20 psu and lower, but was almost above 90% when salinity was 25 psu and higher. The embryo development rate was below 60% when salinity was 25 psu and lower, but was above 80% when salinity was between 30 and 35 psu. The fertilization and embryo development rates in the control condition(not including Cu and Cd) were greater than 90%, but decreased a high negative correlation with the increasing of Cu(r=-0.80, r=-0.78) and Cd(r=-0.90, r=-0.82) concentrations, respectively. The fertilization and embryo development rates were significantly inhibited in the addition of Cu($EC_{50}$=17.30 ppb, $EC_{50}$=10.32 ppb) and Cd($EC_{50}$=364.57 ppb, $EC_{50}$=244.04 ppb), respectively. These results suggest that salinity concentrations for successful fertilization and normal embryogenesis of H. pulcherrimus are above 25 psu and 30 psu, respectively, and the biological assays of fertilization and embryo development rates using H. pulcherrimus are useful methods for the ecological toxicity test of marine pollution elements.

• Journal of Ecology and Environment
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• v.33 no.3
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• pp.245-251
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• 2010

We evaluated the interaction between Cd and Zn in the bioaccumulation of seven clones of Salix caprea, which were exposed both to Cd and Zn alone and to a combination of Cd and Zn. Cadmium (Cd) and Zn concentration in the four treatments were administered in the following order: root > leaf > stem, and obvious differences were noted among the treatments and clones. The leaf Cd concentration of clone BH2 and stem Cd concentration of clone BH5 in the combined Cd and Zn treatment group was increased by 62% and 110%, respectively, relative of that of the Cd alone treatment group. On the other hand, the leaf and stem Zn concentration of clone BH8 in the combined Cd and Zn treatment group was reduced by 66% and 61%, respectively, relative to that of the Zn alone treatment group. Translocation of Cd and Zn from the root was higher in the leaf than in the stem, and the combined Cd and Zn treatment stimulated the translocation of Cd from the root to the leaf and stem, whereas it suppressed the translocation of Zn from the root to the leaf and stem. Therefore, the interaction effects were considered strongly synergistic with Cd in the presence of Zn, but proved antagonistic to Zn in the presence of Cd in the combined Cd and Zn treatment group. The phytoremediation potentials of the seven clones, which were estimated from standard indices of Cd and Zn concentration in Cd and Zn alone and the combined Cd and Zn treatment groups, were highest in clone BH3, and lowest in clone BH5. Therefore, we recognize S. caprea as an appropriate material for phytoremediation, and this is particularly the case with clone BH3. However, further research will be required to evaluate the effects of Cd and Zn on the physiological changes as well as tolerance mechanisms against metal toxicity in S. caprea clones.