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Enhanced (R)-2-(4-Hydroxyphenoxy)Propionic Acid Production by Beauveria bassiana: Optimization of Culture Medium and H2O2 Supplement under Static Cultivation

  • Hu, Hai-Feng;Zhou, Hai-Yan;Wang, Xian-Lin;Wang, Yuan-Shan;Xue, Ya-Ping;Zheng, Yu-Guo
    • Journal of Microbiology and Biotechnology
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    • v.30 no.8
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    • pp.1252-1260
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    • 2020
  • (R)-2-(4-hydroxyphenoxy)propionic acid (HPOPA) is a key intermediate for the preparation of aryloxyphenoxypropionic acid herbicides (R-isomer). In order to improve the HPOPA production from the substrate (R)-2-phenoxypropionic acid (POPA) with Beauveria bassiana CCN-A7, static cultivation and H2O2 addition were attempted and found to be conducive to the task at hand. This is the first report on HPOPA production under static cultivation and reactive oxygen species (ROS) induction. On this premise, the cultivation conditions and fermentation medium compositions were optimized. As a result, the optimal carbon source, organic nitrogen source, and inorganic nitrogen source were determined to be glucose, peptone, and ammonium sulfate, respectively. The optimal inoculum size and fermentation temperature were 13.3% and 28℃, respectively. The significant factors including glucose, peptone, and H2O2, identified based on Plackett-Burman design, were further optimized through Central Composite Design (CCD). The optimal concentrations were as follows: glucose 38.81 g/l, peptone 7.28 g/l, and H2O2 1.08 g/l/100 ml. Under the optimized conditions, HPOPA titer was improved from 9.60 g/l to 19.53 g/l, representing an increase of 2.03-fold. The results obtained in this work will provide novel strategies for improving the biosynthesis of hydroxy aromatics.

Trend and Improvement for Privacy Protection of Future Internet (미래 인터넷 기술의 Privacy 보호 기술 동향 및 개선)

  • Kim, DaeYoub
    • Journal of Digital Convergence
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    • v.14 no.6
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    • pp.405-413
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    • 2016
  • To solve various problems of the Internet as well as to enhance network performance, various future Internet architectures utilize cached data in network nodes or in proxy servers. Named-data networking (NDN), one of future Internet architectures, implements in-network data caching functionality, and then responds itself to request messages. However, it can cause users' privacy invasion that the publisher of data can not engage in the sharing/using process of the data anymore after the data was cached in-network. So NDN implements both encryption-based access control and group access control. But, since such access control schemes need to exchange additional messages in order to search for a proper access control list and keys, it causes inefficiency. This paper surveys the access control schemes of NDN, and then proposes an improved scheme.

Cyr61/CCN1 Overexpression Induces Epithelial-Mesenchymal Transition Leading to Laryngeal Tumor Invasion and Metastasis and Poor Prognosis

  • Liu, Ying;Zhou, Yan-Dong;Xiao, Yu-Li;Li, Ming-Hua;Wang, Yu;Kan, Xuan;Li, Qiu-Ying;Lu, Jian-Guang;Jin, De-Jun
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.7
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    • pp.2659-2664
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    • 2015
  • Background: To examine the expression of cysteine-rich 61 (Cyr61/CCN1) protein in laryngeal squamouscell carcinoma (LSCC) tissues, and its relationship with the tumor epithelial-mesenchymal transition (EMT), invasion, metastasis, and prognosis. Materials and Methods: Immunohistochemistry was used to detect the expressions of Cyr61, Vimentin (Vim), and E-cadherin (E-cad) in 88 cases of LSCC tissues and 30 cases of tumor-adjacent normal tissues. Vim and E-cad were used as mesenchymal and epithelial markers, respectively, to determine the relationship between Cyr61 expression and the EMT of LSCC cells. In addition, clinical and histopathological data were combined to analyze the relationship between the positive-expression rates of Cyr61, Vim and E-cad and LSCC invasion, metastasis and prognosis. Results: In LSCC tissues, Vim expression rate was significantly higher than that of the tumor-adjacent tissues, whereas E-cad expression rate was significantly lower than that of the tumor-adjacent tissues. The Vim expression rate was significantly higher in stages T3 and T4 than in stages T1 and T2 LSCC tissues, whereas E-cad expression rate was significantly lower in stages T3 and T4 than in stages T1 and T2 LSCC tissues. Compared to the group without lymph node metastasis, the Vim expression rate was significantly higher and the E-cad expression rate was significantly lower in the group with lymph node metastasis. The expression rate of Cyr61 was significantly higher in LSCC tissues than in the tumor-adjacent normal tissues. In addition, the Cyr61 expression rate was higher in stages T3 and T4 than in stages T1 and T2 LSCC, and higher in the group with lymph node metastasis than in the group without lymph node metastasis. The Vim expression rate was significantly higher in the Cyr61 positive group than in the Cyr61 negative group, whereas the E-cad expression rate was significantly higher in the Cyr61 negative group than in the Cyr61 positive group. Survival analysis indicated that survival rates of Cyr61 positive, Vim positive and E-cad negative groups were significantly lower than that of Cyr61 negative, Vim negative and E-cad positive groups, respectively. Conclusions: Cyr61 expression is closely associated with LSCC invasion and lymph node metastasis. Overexpression of Cyr61 may induce EMT and therefore leads to LSCC invasion and metastasis and poor prognosis. Cyr61 may become a new maker for clinical prediction of LSCC invasion and metastasis and a new target for LSCC treatment.

Contents Routing in the OpenFlow-based Wireless Mesh Network Environment (OpenFlow기반 무선 메쉬 네트워크 환경에서의 컨텐츠 라우팅)

  • Kim, Won-Suk;Chung, Sang-Hwa;Choi, Hyun-Suk;Do, Mi-Rim
    • Journal of KIISE
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    • v.41 no.10
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    • pp.810-823
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    • 2014
  • The wireless mesh network based on IEEE 802.11s provides a routing based on a destination address as it inherits legacy internet architecture. However, this architecture interested in not 'what' which is originally the users goal but 'where'. Futhermore, because of the rapid increase of the number of mobile devices recently, the mobile traffic increases geometrically. It reduces the network effectiveness as increasing many packets which have same payload in the situation of many users access to the same contents. In this paper, we propose an OpenFlow-based contents routing for the wireless mesh network(WMN) to solve this problem. We implement contents layer to the legacy network layer which mesh network uses and the routing technique based on contents identifier for efficient contents routing. In addition we provide flexibility as we use OpenFlow. By using this, we implement caching technique to improve effectiveness of network as decreasing the packet which has same payload in WSN. We measure the network usage to compare the flooding technique, we measure the delay to compare environment using caching and non caching. As a result of delay measure it shows 20% of performance improve, and controller message decrease maximum 89%.