• Korean Journal of Organic Agriculture
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• v.21 no.4
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• pp.753-767
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• 2013

Soil contamination has continuously increased worldwide, thus the remediation for the contaminated soils has risen steadily. However, the consideration of ecological safety for the remediated soils and their agricultural uses has been very limited. Therefore, this study was to investigate the influences of selected environmentally-friendly agricultural materials, organic by-product fertilizer (OF), charcoal (CC), and biochar (BC), as soil conditioners for improving poor chemical properties of fuel-oil removed soil by land farming technique. Two different remediated soils, remediated soil A (RSA) and remediated soil B (RSB), were selected. Soil texture of both RSA and RSB was sandy loam. The chemical properties of RSA and RSB were as follows: soil pHs of 8.5 and 8.7, soil organic matter contents of 7.4 and 5.5g $kg^{-1}$, total nitrogen contents of 0.26 and 0.10g $kg^{-1}$, available phosphorus concentrations of 7.2 and 4.4mg $kg^{-1}$, and exchangeable calcium concentrations of 14.8 and $11.7cmol_c$ $kg^{-1}$, respectively. Results of the properties were not reached for the optimal values for cultivating crops that were recommended by National Academy of Agricultural Science at Rural Development Administration in Korea. However, after applying OF, CC, and BC, the chemical properties of soils were selectively improved, which were that soil organic matter content and available phosphorus concentration increased, whereas the soil pH were not changed. In particular, the chemical properties were positively changed more with the application of 5.0% biochar. Thus, continuous management of the remediated soils with applying the eco-friendly agricultural materials can improve the quality of reme-diated soils.

• Membrane and Water Treatment
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• v.13 no.3
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• pp.129-137
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• 2022

In this study, the removal of Ciprofloxacin (CPX) from aqueous solutions was investigated by a new activated carbon adsorbent prepared from orange peel (ACOP) with chemical activation using ZnCl₂. The physicochemical properties of orange peel activated carbon were characterized by proximate and ultimate analysis, scanning electron microscopy, BET surface area determination and Fourier transformation infrared spectroscopic studies. According to Brunauer-Emmett-Teller isotherm and non-local-density functional theory, the cumulative surface area, pore volume and pore size of ACOP were determined as 1193 m² g^-1, 0.83 cc g^-1 and 12.7 Å, respectively. The effects of contact time, pH, temperature and ACOP dose on the batch adsorption of CPX were studied. Adsorption equilibrium data of CPX with ACOP were found to be compatible with both the Langmuir and Freundlich isotherms. CPX adsorption capacity of ACOP was calculated as 181.8 mg g^-1 using Langmuir isotherm. The CPX adsorption kinetics were found to be harmonious with the pseudo-second-order kinetic model. Conclusively, ACOP can be assessable as an effective adsorbent for the removal of ciprofloxacin (CPX) from aqueous solutions.

• The Korean Journal of Nuclear Medicine
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• v.5 no.2
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• pp.19-40
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• 1971

Since the iron balance is maintained by regulated intestinal absorption rather than regulated excretion, there have been many reports concerning the factors which may influence the intestinal iron absorption. As the liver is the largest iron storage organ of the body, any hepatocellular damage may result in disturbances in iron metabolism, e,g., frequent co-existence of hemochromatosis and liver cirrhosis, or elevated serum iron level and increased iron absorption rate in patients with infectious hepatitis or cirrhosis. In one effort to demonstrate the influence of hepatocellular damage on intestinal iron absortion, the iron absorption rate was measured in the rabbits whose livers were injured by a single subcutaneous injection of carbon tetrachloride (doses ranging from 0.15 to 0.5cc per kg of body weight) or by a single irradiation of 2,000 to 16,000 rads with $^{60}Co$ on the liver locally. A single oral dose of $1{\mu}Ci\;of\;^{59}Fe$-citrate with 0.5mg of ferrous citrate was fed in the fasting state, 24 hours after hepatic damage had been induced, without any reducing or chelating agents, and stool was collected for one week thereafter. Serum iron levels, together with conventional liver function tests, were measured at 24, 48, 72, 120 and 168 hours after liver damage had been induced. All animals were sacrificed upon the completing of the one week's test period and tissue specimens were prepared for H-E and Gomori's iron stain. Following are the results. 1. Normal iron absorption rate of the rabbit was $41.72{\pm}3.61%$ when 0.5mg of iron was given in the fasting state, as measured by subtracting the amount recovered in stool collected for 7 days from the amount given. The test period of 7 days is adequate, for only 1% of the iron given was excreted thereafter. 2. The intestinal iron absorption rate and serum iron level were significantly increased when the animal was poisoned by a single subcutaneous injection of 0.15cc. per kg. of body weight of carbon tetrachloride or more, or the liver was irradiated with a single dose of 12,000 rads or more. The results of liver function tests which were done simultaneously remained within normal limit except SGOT and SGPT which were somewhat increased. 3. In each case, there has been good correlation between the extent of liver cell damage and degree of increased iron absorption rate or serum iron level. 4. The method of liver damage appeared to make no obvious difference in the pattern of iron deposit in liver. This may be partly due to the fact that tissue specimens were obtained too late, for by this time the elevated serum iron level had returned within normal range and the pathological changes were almost healed. 5. The possible factors and relationship between intestinal iron absorption and hepatic parenchymal cell damage has been discussed.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.20 no.2
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• pp.139-147
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• 1998

This study has been performed to evaluate the relationship between the remained mineral components in a decalcified bone matrix and an ectopic bone formation efficiency. The freezed rat diaphyseal cortical bones measuring 0.5cm in length were demineralized in heated 0.6N HCl at $60^{\circ}C$ for 5, 10, 15, 20, 25, 30, 35, 40 minutes, respectively, using a controlled heat ultrasonic cleaner. Each 1cc of decalcifying solution taken during decalcification procedure was used to calculate calcium content using calcium dignostics kit under 600nm of spectrophotomer. After decalcification, each specimen was also weighed. Then each prepared specimen was implanted into the dorsal pouch of 24 Sprague-Dawley rats divided into 8 groups by time course. The implants were harvested at 1, 2, and 3 weeks and prepared for routine H-E stain specimens to evaluate osteogenic activity. The results are as follows: 1. There was statistical significant difference in change of calcium concentration up to demineralization of 30 minutes and each allogenic bones decalcifed up to 20 minutes revealed 99.65% of decalcification in average. 2. There was statistical significant difference in change of weight in demineralized allogenic bone up to 20 minutes treatment but, no significant change was noted after that time. 3. The histologic analysis revealed active ectopic bone formation in the implanted allografts demineralized for 20, 25, 30 minutes, respectively. However, the other groups of allografts showed relatively poor osteoinductive activity. These findings suggest that complete decalcification with a minimized degeneration of collagen matrix is necessary to induce maximal osteogenesis by decalcified bone allograft.

• Microbiology and Biotechnology Letters
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• v.37 no.1
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• pp.33-41
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• 2009

The crushed fruiting body of Alaskan Porodaedalea pini (Brot.) Murrill (syn. Phellinus pini) was extracted in boiling water for 4 h with the yield of 20.5% in dry mass. This hot-water extract showed significant antiviral activity by inhibiting the plaque formation in HeLa cells by coxackievirus B3 (CVB3) and also showed highest inhibitory effect against neuraminidase activity among water extracts of various mushrooms. From the water extract, the ethanol precipitate (EP) and supernatant fraction (ES) were obtained through 75% ethanol precipitation with the yield of 43.3% and 28.3% in dry mass, respectively. Whereas ES did not show any detectable level of antiviral activity, EP showed significant dose-dependent inhibition of plaque formation by CVB3 in HeLa cells with an $EC_{50}$ (50% effective concentration) of 0.45 mg/mL. The cytotoxicity on HeLa cells by EP was relatively low with the $CC_{50}$ (50% cytotoxic concentration) of 2.25 mg/mL. EP also effectively inhibited neuraminidase activity in a dose-dependent manner showing up to 75% inhibition at 1.7 mg/mL. These results suggest that the hot-water extract and its EP of P. pini fruiting body can be a candidate for the development of a potent broad-range antiviral agent against influenza virus(Flu) as well as CVB3. The major active component of EP was shown to be a heteropolysaccharide-protein complex containing glucose as the main sugar residue with mole percentage of 79.8% and other sugars like galactose (19.2%), xylose (17%), mannose (5.8%), and fucose (4.6%) and a small portion (12.7%, in mass) of protein.

• Clinical and Experimental Reproductive Medicine
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• v.26 no.2
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• pp.239-249
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• 1999

The role of amino acids in culture media for IVF-ET was examined in a total of 76 cycles. Patients received clomiphene citrate (CC) followed by hMG or GnRH-a combined with gonadotropins (FSH/hMG) for controlled ovarian hyperstimulation. Severe male (<$4{\times}10^6$ motile sperm) or age factor (>39 y) patients were excluded in this study. Pregnancy was classified as clinical if a gestational sac or fetal cardiac activity was seen on ultrasound. No significant differences were found in age, duration of infertility, follicle size, the level of $E_2$ on the day of hCG injection, the mean number of oocytes retrieved, total motile sperm count, fertilization rate and the mean number of embryos transferred between bHTF (without amino acids) and mHTF (with amino acids) groups. However, total ampules of gonadotropins were higher (p<0.01) in mHTF group than bHTF group. Significantly (p<0.05) more clinical pregnancies were recorded in mHTF group (13/30) compared with bHTF group (9/46). The multiple pregnancy rates were 11.1% in bHTF group and 7.7% in mHTF group. There were one ectopic pregnancy in mHTF group and one heterotopic pregnancy in bHTF group. Abortion rates were 22.2% in bHTF group and 7.7% in mHTF, respectively. The ongoing pregnancy or livebirth rate was significantly (p<0.05) higher in mHTF group (12/30) than bHTF group (7/46). These results suggest that the addition of amino acids in culture media is essential for culture of zygotes in vitro and adjustment of energy substrates in phosphate-free culture media appears to be beneficial for human IVF-ET procedure.

• Journal of Chest Surgery
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• v.31 no.2
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• pp.108-112
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• 1998

Experimental trials of unilateral lung transplantation in dogs have been attempted and satisfactory results were obtained without any noticeable difficulty in surgical techniques. Fourteen dogs with the body weight of around 25 kg were anesthesized by 20~30 mg/kg of intravenous Entobar,; one was sacrificed to make available blood for use during transplantation for the recipient dog. A mid-sternotomy incision was performed and 20 mg/kg of Prostaglandin E1 was infused through the pulmonary artery and Euro-Collin's(E-C) preservation solution, cooled down to 4$^{\circ}C$, was perfused at the rate of 70cc/kg by a pressure of 30 cmH2O. The heart-lung block was then resected out and promptly immersed in the prepared preservation solution at 4$^{\circ}C$. One lung preserved in the EC solution at 4$^{\circ}C$ was anastomosed to the recipient dog in the order of the pulmonary vein, bronchus then pulmomary artery and the thoracotomy incision was closed after the bleeding control and tube thoracostomy. Then the pneumonectomy in the opposite side was perfomed in the same manner and the tailored lung was transplanted in the order of the pulmonary vein, bronchus, then pulmonary artery. We conclude that in the bilateral sequential lung transplantation, the right lung transplantation should precede to better expose the operative field and to prevent reperfusion injury; also, the cardiopulmonary bypass should be consider for certain appropriate cases.

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 2002.11a
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• pp.131-186
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• 2002

우리나라 유통상토에 대한 물리적 표준분석법을 설정하기 위한 연구를 실시하였다. 국내 시중에 유통되고 있는 53개 품목의 원예용 상토와 9개 품목의 수도용 상토를 대상으로 설정된 분석법을 적용하여 실험하였다. 입자밀도의 측정은 가스형 피크노메타(Micromeritics Co. Model 1305)를 사용하면 속도와 정확성 면에서 매우 유리하였다. 용적밀도는 구미에서 Sandbox법에 의한 용적밀도를 기본으로 하기 때문에 이와 유사한 성적을 얻을 수 있는 다양한 실험을 한 결과, 추다짐법(Plunger compaction method)이 가장 유의성 있는 측정 방법으로 밝혀졌다. 한편 가스형 피크노메타법에 의한 우리나라 유통상토의 입자밀도는 원예용이 1.93, 수도용이 2.43 Mg m$^{-3}$로 나타났으며, 추다짐법에 의한 용적밀도는 원예용이 0.22, 수도용이 1.01 Mg m$^{-3}$로 각각 나타났다. 수분함량은 105$^{\circ}C$ 건조기에서 16시간 건조하여 수도용상토는 (최초 무게-건조 후 무게) / (건조 후 무게) $\times$ 100으로, 원예용상토는 (최초무게 - 건조 후 무게) / (최초 무게) $\times$ 100으로 하였다. 보수력은 주로 저압용으로서 Eijkelkamp사의 Sandbox법을 사용하여 0.5, 1, 3, 5, 7, 10 ㎪의 수분장력 상태의 수분함량을 조사하였다. 포화수리전도도는 직경 5cm$\times$높이 20cm인 아크릴 원통을 이용하여 정수위별에 의한 포화수리전도도를 측정하였다. 신분석법에 의한 유통상토의 수분함량은 원예용이 평균 46.34%, 수도용이 평균 16.89%이었고 원예용 상토의 EAW는 28.4%, WBC 7.01%, OMP는 5.60 ㎪로 나타났으며, 육묘를 위한 적정 수분함량은 44.41%이었고 포화수리전도도는 1.71 cm $min^{-1}$이었다. 상토 평량시 무게를 비교한 결과 상토 부피를 달리하여도 고도의 유의성이 있었고, 상토 20cc의 평균무게는 4.92g(2.08-8.83g)이었다. 상토의 EC 측정시 상토:물의 비율이 1:2(v:v)와 1:5(v:v)분석치에 2.5배한 EC 값과는 고도의 정의 상관관계가 있었으며, 유럽법과 일본법(전농)과의 관계는 고도의 정의 상관관계가 있었다 또한 새로운 분석법(v:v 1:5)으로 측정한 EC의 평균값은 국내산 0.64, 수입산 0.33 dS m$^{-1}$이 었다. 상토의 pH 측정시 상토:물의 비율을 1:2(v:v)와 1:5(v:v)와의 관계에서는 정의 상관관계를 보였으며, 유럽법과 일본법(전농)과의 관계도 역시 고도의 정의 상관관계가 있었고, 신 분석법에(v:v 1:5) 의한 분포비율은 국내산, 수입산모두 pH 6.1-6.5의 범위에서 가장 높았다.

• Korean Journal of Animal Reproduction
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• v.23 no.4
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• pp.337-345
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• 1999

The present study was conducted to investigate the effects of various co-culture systems and supplemented protein sources on the in vitro development of bovine IVF embryos. Bovine cumulus oocyte complexes (COCs) were matured and fertilized in vitro. Presumptive zygotes with cumulus cells were transferred to TCM-199 or CRlaa containing 10% FBS or 3mg/$m\ell$ BSA, and cultured for 36~40 hr. After primary culture, cleaved embryos were co-cultured with cumulus cells(CC), bovine oviduct epithelial cells(BOEC) or Buffalo rat liver cells (BRLC) in TCM-199 or CRlaa supplemented with FBS or BSA respectively, for further 6 days. Cleavage rate increased with BSA(P＜0.01) in the both TCM-199(79%) or CRlaa(74%) When embryos were co-cultured with CC or BOEC in TCM-199, blastocyst development was enhanced with BSA(40% and 43%) compared to FBS (22% and 29%) , whereas in CRlaa no difference observed between BSA(40% and 39%) and FBS (40% and 42%). When embryos were co-cultured with BRLC monolayer, FBS enhanced the blastocyst development (P＜0.05) compared to BSA in both TCM-199(41% vs 31%) and CRlaa (44% vs 37%). The result of the present study showed that the cleavage rate of bovine IVF embryos increased with BSA, The result also showed that BSA can enhance the development of IVF embryos in co-culture with CC or BOEC in TCM-199, suggesting the in vitro development is affected by the medium and supplemented protein sources in co-culture with somatic cells.

• Korean Journal of Soil Science and Fertilizer
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• v.40 no.5
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• pp.418-428
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• 2007

Physical and chemical properties of the aggregate by-products including sludge and crushed dust samples collected from the 21 private companies throughout the country were analyzed to evaluate possible usage of the by-products as artificial soil materials for plantation. The pH of the materials ranged from 8.0 to 11.0. The organic matter content was $2.85g\;kg^{-1}$, and the total nitrogen content and available phosphate content were low as 0.7 percents and $12.98mg\;kg^{-1}$, respectively. Exchangeable $Ca^{2+}$, $Mg^{2+}$, $K^+$, and $Na^+$ were 2.29, 0.47, 0.02 and $0.05cmol\;kg^{-1}$, respectively. Heavy metal contents were lower than the limits regulated by environmental law of Korea. Textural analysis showed that most of the materials were silt loam with low water holding capacity ranged from 0.67 to 7.41 percents, and with low hydraulic conductivity ranged from 0.4 to $2.8m\;s^{-1}$. Mineralogical analysis showed that the aggregate by product materials were mostly composed of silicate, alumina and ferric oxides except calcium oxide dominant materials derived from limestones. The primary minerals were quartz, feldspars and dolomites derived from granite and granitic gneiss materials. Some samples derived from limestone material showed calcite and graphite together with the above minerals. According to the result, it can be concluded that the materials could be used as the artificial soil material for plantation after proper improvement of the physico-chemical properties and fertility.