• Title/Summary/Keyword: CBM

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Roles of Carbohydrate-Binding Module (CBM) of an Endo-β-1,4-Glucanase (Cel5L) from Bacillus sp. KD1014 in Thermostability and Small-Substrate Hydrolyzing Activity

  • Lee, Jae Pil;Shin, Eun-Sun;Cho, Min Yeol;Lee, Kyung-Dong;Kim, Hoon
    • Journal of Microbiology and Biotechnology
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    • v.28 no.12
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    • pp.2036-2045
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    • 2018
  • An endo-${\beta}$-1,4-glucanase gene, cel5L, was cloned using the shot-gun method from Bacillus sp.. The gene, which contained a predicted signal peptide, encoded a protein of 496 amino acid residues, and the molecular mass of the mature Cel5L was estimated to be 51.8 kDa. Cel5L contained a catalytic domain of glycoside hydrolase (GH) family 5 and a carbohydrate-binding module family 3 (CBM_3). Chromatography using HiTrap Q and CHT-II resulted in the isolation of two truncated forms corresponding to 50 (Cel5L-p50) and 35 kDa (Cel5L-p35, CBM_3-deleted form). Both enzymes were optimally active at pH 4.5 and $55^{\circ}C$, but had different half-lives of 4.0 and 22.8 min, respectively, at $70^{\circ}C$. The relative activities of Cel5L-p50 and Cel5L-p35 for barley ${\beta}$-glucan were 377.0 and 246.7%, respectively, compared to those for carboxymethyl-cellulose. The affinity and hydrolysis rate of pNPC by Cel5L-p35 were 1.7 and 3.3 times higher, respectively, than those by Cel5L-p50. Additions of each to a commercial enzyme set increased saccharification of pretreated rice straw powder by 17.5 and 21.0%, respectively. These results suggest CBM_3 is significantly contributing to thermostability, and to affinity and substrate specificity for small substrates, and that these two enzymes could be used as additives to enhance enzymatic saccharification.

A Predictive System for Equipment Fault Diagnosis based on Machine Learning in Smart Factory (스마트 팩토리에서 머신 러닝 기반 설비 장애진단 예측 시스템)

  • Chow, Jaehyung;Lee, Jaeoh
    • KNOM Review
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    • v.24 no.1
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    • pp.13-19
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    • 2021
  • In recent, there is research to maximize production by preventing failures/accidents in advance through fault diagnosis/prediction and factory automation in the industrial field. Cloud technology for accumulating a large amount of data, big data technology for data processing, and Artificial Intelligence(AI) technology for easy data analysis are promising candidate technologies for accomplishing this. Also, recently, due to the development of fault diagnosis/prediction, the equipment maintenance method is also developing from Time Based Maintenance(TBM), being a method of regularly maintaining equipment, to the TBM of combining Condition Based Maintenance(CBM), being a method of maintenance according to the condition of the equipment. For CBM-based maintenance, it is necessary to define and analyze the condition of the facility. Therefore, we propose a machine learning-based system and data model for diagnosing the fault in this paper. And based on this, we will present a case of predicting the fault occurrence in advance.

Machine Diagnosis and Maintenance Policy Generation Using Adaptive Decision Tree and Shortest Path Problem (적응형 의사결정 트리와 최단 경로법을 이용한 기계 진단 및 보전 정책 수립)

  • 백준걸
    • Journal of the Korean Operations Research and Management Science Society
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    • v.27 no.2
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    • pp.33-49
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    • 2002
  • CBM (Condition-Based Maintenance) has increasingly drawn attention in industry because of its many benefits. CBM Problem Is characterized as a state-dependent scheduling model that demands simultaneous maintenance actions, each for an attribute that influences on machine condition. This problem is very hard to solve within conventional Markov decision process framework. In this paper, we present an intelligent machine maintenance scheduler, for which a new incremental decision tree learning method as evolutionary system identification model and shortest path problem as schedule generation model are developed. Although our approach does not guarantee an optimal scheduling policy in mathematical viewpoint, we verified through simulation based experiment that the intelligent scheduler is capable of providing good scheduling policy that can be used in practice.

Analysis Procedure For Customer Behavior Model Using Web-Log (웹 로그를 이용한 고객행동모델 분석방법에 관한 연구)

  • Seo, Jang-Hoon;Shim, Sang-Yong;Yoo, Woong-Jae
    • Proceedings of the Safety Management and Science Conference
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    • 2006.11a
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    • pp.299-307
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    • 2006
  • In this report, we provide the focus on suggesting a method of estimating and measurement of CBM(Customer Behavior Model). Through the use of internet, a new trend of business for e-CRM on B2C Web Site known as EC has emerged. The purpose of this study is to identify the relationship between the customers of a shopping mall and CBM characteristics. It can be used to gain a better understanding of customers. From this we can determine trends, and so refine business toward customer's needs and target new products to particular customer groups. Result shows that there is a significant relationship between the customers pattern of shopping mall and CBM, CVM(Customer Visit Model).

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A Study on the Expertment of Selective Frequency Hopping System (선택성 주파수 호핑 시스템의 실현에 관한 연구)

  • 정용주;김원후
    • Proceedings of the Korean Institute of Communication Sciences Conference
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    • 1987.04a
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    • pp.201-205
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    • 1987
  • In FH-SS systems when the bopping band is enough wide to onerlap with conventional band limited communication cethod (CBM). The portion of suchacts as an interfering signal. Thus it is gard to use them all together. This paper presents how the frequency gopping systems can simultaneously share the same band with CBM. The proposed mithod is that the frequency gopping band can arbitraily controlled by setting the specific input bith of digital frequency synthesizer to logical zero state We realized this by putting the hopping band Controller between pseudeo random generater and frequency synthesizer.

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Effects of Supplementary Blood Meal on Carnosine Content in the Breast Meat and Laying Performance of Old Hens

  • Namgung, N.;Shin, D.H.;Park, S.W.;Paik, I.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.23 no.7
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    • pp.946-951
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    • 2010
  • The objective of this research was to evaluate the effects of dietary supplementation of blood meal (BM) as a source of histidine, and magnesium oxide (MgO) as a catalyst of carnosine synthetase, on carnosine (L-Car) content in the chicken breast muscle (CBM), laying performance, and egg quality of spent old hens. Four hundred eighty laying hens (Hy-Line$^{(R)}$ Brown), 95wk old, were allotted randomly into five replicates of six dietary treatments: T1; 100% basal diet, T2; 100% basal diet+MgO, T3; 97.5% basal diet+2.5% BM, T4; 97.5% basal diet+2.5% BM+MgO, T5; 95% basal diet+5% BM, T6; 95% basal diet+5% BM+MgO. Magnesium oxide was added at 0.3% of diets. The layers were fed experimental diets for 5wk. There were no significant differences in the weekly L-Car content in CBM among all treatments during the total experimental period, but some of the contrast comparisions showed higher L-Car in CBM of T6. The L-Car contents linearly decreased (p<0.01 or p<0.05) as the layers got older except in T4 (p>0.05). There were significant differences in egg weight (p<0.01) and soft and broken egg ratio (p<0.05). The control (T1) was highest in egg weight and T6 was lowest in soft and broken egg ratio. Among the parameters of egg quality, there were significant differences in eggshell strength (p<0.01) and egg yolk color (p<0.05). Magnesium oxide supplementation increased the eggshell strength and BM tended to decrease egg yolk color. Eggshell color, eggshell thickness, and Haugh unit were not influenced by BM and MgO. In conclusion, BM and MgO did not significantly influence the L-Car in CBM of spent layers. The L-Car content rapidly decreased as the layers became senescent. Eggshell strength was increased by MgO supplementation.

Development of Intelligent System to Select Production Method in Coalbed Methane Reservoir (석탄층 메탄가스 저류층의 생산방법 선정을 위한 지능형 시스템 개발)

  • Kim, Chang-Jae;Kim, Jung-Gyun;Lee, Jeong-Hwan
    • Journal of the Korean Institute of Gas
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    • v.18 no.2
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    • pp.1-9
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    • 2014
  • To develop a coalbed methane(CBM) reservoir, it is important to apply production methods such as drilling, completion, and stimulation which coincide with coal properties. However, the reliability of the selected resulted in most of CBM field is not enough to accept because the selection of production method has been done by empirical decision. As the result, the empirical decision show inaccurate results and need to prove using simulation whether it was true exactly. In this study, the intelligent system has been developed to assist the selection of CBM production method using artificial neural network(ANN). Before the development of the system, technical screening guideline was analyzed by literature survey and the system to select drilling and completion method, and hydraulic fracture fluid was developed by utilizing the guideline. The result as a validation of the developed system showed a high accuracy. In conclusion, it has been confirmed that the developed system can be utilized as a effective tool to select production method in CBM reservoir.

Development of Telemedicine which is a CBM based Collaborative Multimedia System on LAN Environment (LAN환경에서의 CBM기반의 상호 참여형 멀티미디어 시스템인 원격 진료 시스템의 개발)

  • Kim, Seok-Su
    • The Transactions of the Korea Information Processing Society
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    • v.4 no.5
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    • pp.1153-1161
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    • 1997
  • In this paper, we propose Teloemedicine system on CBM(Computer Based Multimedia) based collaborative multimedia which supports special medicine and collaborative medicine with DB, face-to-face dffedt.This system is an application developed by SDK (Software Development Kit) of DooRae(Distributed Objected Oriented Multimedia Application Crafting Environment for Collaborative)framework.And this system is devel-Oriented Multimedia Applecation Crafting Environment for Collaborative) framework, And this system is devel-oped on windows 95 and windows NT.The scenario of this system is limited within only hospital(LAN), but it is possibloe to support many applications development in various nerwork(MAN, PSYN, WAN)on DooRae.This system has a smoth interaction by video and audio, multiple session, multiple participation, application sharing, toolbox including ICON and whiteboard.Also this system supports realo or non-real type without con-straint of the and space.

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A Study on the Multimedia Communication and Distance Education for Information Society (정보화 사회에 따른 멀티미디어 통신과 원격교육에 관한 연구)

  • 정재영;김석수
    • Journal of the Korea Society of Computer and Information
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    • v.4 no.1
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    • pp.15-20
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    • 1999
  • In this paper, we propose some essential requirements for CBM(Computer Based Multimedia) based distance education. This is a class of multimedia distance education in which the multimedia technique is combined with the distance home education application that is real time collaborative application on cyber-space using the information super highway. Requirements in this paper is observed standard at ITU(International Telecommunication Union). international standardization organization for multimedia communication. And. a structure for integrated home information service on the information super highway is based on IEEE 1394 serial communication and POF(Plastic Optical Fiber) home backbone that is study for application service according to the multimedia based distance home study system.

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Fielding a Structural Health Monitoring System on Legacy Military Aircraft: a Business Perspective

  • Bos, Marcel J.
    • Journal of the Korean Society for Nondestructive Testing
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    • v.35 no.6
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    • pp.421-428
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    • 2015
  • An important trend in the sustainment of military aircraft is the transition from preventative maintenance to condition based maintenance (CBM). For CBM, it is essential that the actual system condition can be measured and the measured condition can be reliably extrapolated to a convenient moment in the future in order to facilitate the planning process while maintaining flight safety. Much research effort is currently being made for the development of technologies that enable CBM, including structural health monitoring (SHM) systems. Great progress has already been made in sensors, sensor networks, data acquisition, models and algorithms, data fusion/mining techniques, etc. However, the transition of these technologies into service is very slow. This is because business cases are difficult to define and the certification of the SHM systems is very challenging. This paper describes a possibility for fielding a SHM system on legacy military aircraft with a minimum amount of certification issues and with a good prospect of a positive return on investment. For appropriate areas in the airframe the application of SHM will reconcile the fail-safety and slow crack growth damage tolerance approaches that can be used for safeguarding the continuing airworthiness of these areas, combining the benefits of both approaches and eliminating the drawbacks.