• Title/Summary/Keyword: CAT-2

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Effects of Ginseng Radix on sperm count and CatSper3, 4 proteins expression in Male Mice (인삼(人蔘)이 수컷 생쥐의 정자 수와 CatSper3, 4 단백질 발현에 미치는 영향)

  • Park, Eun-Hwa;Kim, Do-Rim;Park, Seong-Kyu;Chang, Mun-Seog
    • The Korea Journal of Herbology
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    • v.27 no.2
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    • pp.47-51
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    • 2012
  • Objectives : The purpose of this study was to investigate the reproductive effect of Ginseng radix on male mice. Methods : Male C57BL/6J mice were divided into four groups ; normal group (vehicle-treated, n=8), Ginseng radix treatment group (100, 500, 1000 mg/kg, n=8). Ginseng radix extract was treated for 5 weeks. After treatment each group was examined for assessment of sperm count and CatSper protein expression using computer assisted semen analysis (CASA) system and the immunofluorescence. Results : Sperm count of normal and Ginseng radix extract treated group were 287.57 vs. 371.62, 364.83, $343.29{\times}10^6$, respectively. The CatSper3, 4 proteins expression of Ginseng radix treated group were significantly increased than that of normal group. Conclusions : These findings suggest that the Ginseng radix improves male reproductive function by increasing sperm count and CatSper protein expression.

Splenic Mast Cell Tumors in Two Cats

  • Jung, Ji-Youl;Kim, Nak-Hyoung;Yim, So-Jeong;Hong, Kyung-Hwa;Park, Ja-Sil;Kim, Jae-Hoon
    • Journal of Veterinary Clinics
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    • v.38 no.2
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    • pp.82-84
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    • 2021
  • Two 11-year-old cats, female Korean shorthair cat and male Siamese cat, with abdominal distention were presented to the local animal hospitals. Radiographic and ultrasonographic examinations revealed moderate to severe splenomegaly in both cats. In Korean shorthair cat, multiple masses were also existed on the anal and facial skin. Surgically excised whole spleens of two cats were requested for histopathologic examination. Histopathologically, numerous neoplastic round cells with cytoplasmic fine granules were widely infiltrated in the splenic parenchyma. The cytoplasmic granules were metachromatic on toluidine blue staining. These splenic masses were diagnosed as splenic mast cell tumors. Among them, Korean shorthair cat was remained healthy for at least 1 year after splenectomy. Because of no visiting of owner, we were only able to know the information for Siamese cat until 10 months after the splenectomy. To our best knowledge, this is the first detail case reports for splenic mast cell tumors in cats in Korea.

The Relationship between Structural Denaturation of Antioxidative Enzymes and Their Enzyme Activity due to Repeated Exposure to UV-A (UV-A 반복노출로 인한 항산화효소의 구조변성과 효소활성의 상관관계)

  • Park, Mijung;Yoo, Hyo Jung;Kim, Jong Chan;Kim, So Ra
    • Journal of Korean Ophthalmic Optics Society
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    • v.20 no.1
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    • pp.75-81
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    • 2015
  • Purpose: The present study was conducted to investigate whether the changes in structure and activity of antioxidative enzymes, superoxide dismutase(SOD) and catalase(CAT) present in the eyes appeared when they were repeatedly exposed to UV-A, and reveal the correlation of these changes. Methods: Each enzyme solution was prepared from the standardized SOD and CAT, and repeatedly exposed to UV-A of 365 min under the condition of 30 minutes, 1 hour and 2 hours a day over 1, 2, 3, 4 and 5 days. Structural denaturation of SOD and CAT induced by repeat UV-A irradiation was confirmed by the electrophoretic analysis, and their enzyme activity was determined by the colorimetric assay using the proper assay kit. Results: SOD exposed repeatedly to UV-A showed the polymerization pattern through the electrophoretic analysis when it was repeatedly exposed under the condition of at least 1 hour a day however, the change of its activity was found to be less than 12%. On the other hand, CAT repeatedly exposed to UV-A showed reduced size of the electrophoretic band which indicated a structure denaturation and its activity was significantly decreased. In the case of that the repeat exposure time was longer, CAT activity was completely lost even though some enzyme band was shown in the electrphoretic analysis. Conclusions: From these results, it was revealed that the degree and pattern in structural denaturation of antioxidative enzymes differently appeared according to the type of enzyme, and the degree of structural denaturation was not always consistent with the reduction in enzyme activity.

Clinical, virological, imaging and pathological findings in a SARS CoV-2 antibody positive cat

  • Ozer, Kursat;Yilmaz, Aysun;Carossino, Mariano;Ozturk, Gulay Yuzbasioglu;Bamac, Ozge Erdogan;Tali, Hasan E.;Mahzunlar, Egemen;Cizmecigil, Utku Y.;Aydin, Ozge;Tali, Hamid B.;Yilmaz, Semaha G.;Mutlu, Zihni;Kekec, Ayse Ilgin;Turan, Nuri;Gurel, Aydin;Balasuriya, Udeni;Iqbal, Munir;Richt, Juergen A.;Yilmaz, Huseyin
    • Journal of Veterinary Science
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    • v.23 no.4
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    • pp.52.1-52.7
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    • 2022
  • This paper reports a presumptive severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in a cat. A cat with respiratory disease living with three individuals with coronavirus disease 2019 showed bilateral ground-glass opacities in the lung on X-ray and computed tomography. The clinical swabs were negative for SARS-CoV-2 RNA, but the serum was positive for SARS-CoV-2 antibodies. Interstitial pneumonia and prominent type 2 pneumocyte hyperplasia were noted on histopathology. Respiratory tissues were negative for SARS-CoV-2 RNA or antigen, but the cat was positive for feline parvovirus DNA. In conclusion, the respiratory disease and associated pathology in this cat could have been due to exposure to SARS-CoV-2.

Ehlers-Danlos Syndrome with Classical Subtype in a Cat

  • Jihyun Kim;Yunji Sul;Jaewon Lee;Sooa Yoon;Seungjin Lee;Woojin Song;Youngmin Yun
    • Journal of Veterinary Clinics
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    • v.41 no.2
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    • pp.101-105
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    • 2024
  • Ehlers-Danlos syndrome (EDS) is a rare genetic disorder in dogs and cats and has been mostly reported in purebred cats. In this study, we report a case of a 1-year-old castrated male Korean shorthair cat, who presented with multiple small skin tears and bruises distributed over the entire trunk area. The cat's skin was hyperextensible and easily torn with gentle touch. The skin extensibility index of the cat was 25%, indicating the possibility of EDS. The cat exhibited no signs of pruritus or inflammation, and no underlying disease was found. However, radiography revealed hip joint subluxation and arthritis. Following this, biopsy of the lacerated skin was performed. Histopathological examination of the skin revealed that in the dermis adjacent to the lesions, the collagen fibers were irregular in size and width, with a slightly thinner epidermis, and increased interfibrillar spaces containing low numbers of scattered well-differentiated fibroblasts and mast cells. Histopathological examination of the skin confirmed EDS. The symptoms observed in the cat, including skin hyperextensibility, multiple bruising, hip joint subluxation, and arthritis, corresponded to the classical subtype of EDS in humans. Thus, this study is a rare report of a classical EDS case in a Korean shorthair cat. This study suggests that skin extensibility index and biopsy are useful diagnostic procedures for confirming EDS in animals until a more definitive genetic test is established.

A Comparative Study of Nurses and Physicians' Attitudes about Complementary and Alternative Therapy (보완대체요법에 대한 간호사와 의사의 태도 비교 연구)

  • Jang, Eun-Hee;Park, Kyung-Sook
    • Korean Journal of Adult Nursing
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    • v.15 no.3
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    • pp.402-410
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    • 2003
  • Purpose: The purpose of this study was to develop a fundamental reference for the clinical implementation of Complementary and Alternative Therapy(CAT) by surveying and analyzing different perception and attitude between nurses and physicians. Method: A total of 167 nurses and 103 physicians from two C university health science centers participated in this survey, and SPSS program with version 10.0 was used to analyze the result of survey. Result: As results, 72.7% nurses and 57.1% physicians showed positive attitude for the concept of CAT, 60.6% nurses and 36.7% physicians responded positively while 2.6% nurses and 25.9% physicians responded negatively for the application of CAT to the clinical practice. There was a meaningful difference between two groups in the belief of therapeutic effectiveness of CAT. 67.7% nurses believed the therapeutic effectiveness of CAT while 38.8% physicians did. For attitudes of nurses and physicians toward CAT showed meaningful difference according to general characteristics: age, gender, marital status, a level of education, position in the organization, religion, and duration of health care service other than inpatient units. Conclusion: In this study, the attitudes of nurses and physicians about CAT showed meaningful difference that nurses were more positive than physicians in application.

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Cloning and Sequence Analysis of Two Catechol-degrading Gene Clusters from a Phenol-utilizing Bacterium Pseudomonas putida SM25

  • Jung, Young-Hee;Ka, Jong-Ok;Cheon, Choong-Ⅰll;Lee, Myeong-Sok;Song, Eun-Sook;Daeho Cho;Park, Sang-Ho;Ha, Kwon-Soo;Park, Young-Mok
    • Journal of Microbiology
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    • v.41 no.2
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    • pp.102-108
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    • 2003
  • A 6.1 kb Sph I fragment from the genomic DNA of Pseudomonas putida SM 25 was cloned into the veetor pUC19. The open reading frame of catB was found to consist of 1,122 nucleotides. The sequence alignment of the catB gene products from different kinds of bacteria revealed an overall identity ranging from 40 to 98%. The catC gene contained an open reading frame of 96 codons, from which a protein with a molecular mass of about 10.6 kDa was predicted. The amino acids in the proposed activesite region of CatC were found to be almost conserved, including the charged residues. Since the catBC genes in P. putida SM25 were tightly linked, the could be regulated under coordinate transcription, and transcribed from a single promoter located upstream of the catB gene, as in P. putida RBI.

R-CAT: Resilient Capacity-Aware Multicast Tree Construction Scheme (R-CAT : P2P기반 스트리밍 환경에서 노드의 능력을 고려한 내구적 멀티캐스트 트리 생성 기법)

  • Kim Eun-Seok;Han Sae-Young;Park Sung-Yong
    • The KIPS Transactions:PartA
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    • v.13A no.2 s.99
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    • pp.147-156
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    • 2006
  • Recently, streaming service accounts for large part of internet traffic and it is becoming the most popular service. Because of P2P's scalability, P2P-based streaming system is proposed. There are frequent leave and join of a node. To overcome the group dynamics, Multiple Multicast Trees Methods were suggested. However, since they did not consider discrepancy in peers' capacity, it may cause the trees to be long and unstable. So we suggest Resilient Capacity-Aware Multicast Tree construction scheme (R-CAT) that promotes superior peer to upper position in the tree and construct more stable and short multicast trees. By simulation we can show that R-CAT cost more overhead packets for tree joining process, but it reduce the end-to-end delay of the resulting tree and the number of packets lost during the node joining and leaving processes much more than SplitStream.

The Effects of the Makgeolri on the Antioxidative Activity in the Endotoxin LPS-treated Rats (내독소인 LPS로 처치된 흰쥐에 대한 막걸리 항산화 활성효과)

  • Kwon, Ryun-Hee;Chae, Go-Yeon;Ho, Boe-Jin
    • Journal of Food Hygiene and Safety
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    • v.26 no.2
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    • pp.166-170
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    • 2011
  • Modem people have begun to have the nationwide interest in the rice wine called Makgeolri which is one of the traditional Korean alcoholic liquors. This study was performed to investigate the effects of San sung Makgeolri extract (SM) on antioxidation together with the determination of pH and dissolved oxygen (DO) in the progress of fermentation in the lipopolysaccharide(LPS)-treated rats. We examined the levels of SOD (superoxide dismutase), CAT (catalase), GPx (glutathione peroxidase) in liver homogenates and the histopathological observations in liver tissue. LPS-treated group markedly decreased the levels of SOD, CAT and GPx. But SM + LPS-treated group significantly increased the levels of them. Furthermore, the antioxidative effects of SM were supported by the histopathological observations in liver tissue which showed severe inflammation and necrosis in LPS-treated group, compared to the attenuated inflammation and necrosis in SM + LPS-treated group. This results suggested that SM could be a candidate of antioxidative material in spite of alcoholic liquors.

CAD/CAM/CAT Turmaround System for Precision Machining (정밀가공을 위한 CAD/CAM/CAT 일괄처리시스템)

  • 안중용;김승철;정성종
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 1993.10a
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    • pp.417-422
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    • 1993
  • In order to minimize turnaround of machining in FMS lines, CAD/CAM/CAT integrated system called MascCAM was developed. Developing enhanced CAM and inspection modules in the MascCAM environment, 2D came, 2 $^{1}$2/ D prismatic parts and 3D free-formed surfaces were able to be automatically designed, manufactured and inspected on the machine tools by using AutoCAM and Z-map. Introducing Z-map technique, the MascCAM was able to be interfaceed with and CAD system. Developed QPPGT module generates a quick and fool-proof inspection work to users. A vertical and a horizontal machining center equipped with FANUC OMC were used for experiments. Performance of the system was confirmed by a large amount of experiments.

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