• Development and Reproduction
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• v.17 no.1
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• pp.9-16
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• 2013

Coactivator-associated arginine methyltransferase 1 (CARM1) is included in the protein arginine methyltransferase (PRMT) family, which methylates histone arginine residues through posttranslational modification. It has been proposed that CARM1 may up-regulate the expression of pluripotency-related genes through the alteration of the chromatin structure. Mouse embryonic stem cells (mESCs) are pluripotent and have the ability to self-renew. The cells are mainly used to study the genetic function of novel genes, because the cells facilitate the transmission of the manipulated genes into target mice. Since the up-regulated methylation levels of histone arginine residue lead to the maintenance of pluripotency in embryos and stem cells, it may be suggested that CARM1 overexpressing mESCs elevate the expression of pluripotency-related genes in reconstituted embryos for transgenic mice and may resist the differentiation into trophectoderm (TE). We constructed a fusion protein by connecting CARM1 and 7X-arginine (R7). As a cell-penetrating peptide (CPP), can translocate CARM1 protein into mESCs. CPP-CARM1 protein was detected in the nuclei of the mESCs after a treatment of 24 hours. Accordingly, the expression of pluripotency-related genes was up-regulated in CPP-CARM1-treated mESCs. In addition, CPP-CARM1-treated mESC-derived embryoid bodies (EBs) showed an elevated expression of pluripotency-related genes and delayed spontaneous differentiation. This result suggests that the treatment of recombinant CPP-CARM1 protein elevates the expression of pluripotency-related genes of mESCs by epigenetic modification, and this protein-delivery system could be used to modify embryonic fate in reconstituted embryos with mESCs.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.16 no.1
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• pp.46-56
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• 1991

In this paper, 16word X 8bit Content Addressable and Reentrant Memory(CARM) is described. This device has 4 operation modes(read, write, match, reentrant). The read and write operation of CARM is like that of static RAM, CARM has the reentrant mode operation where the on chip garbage collection is accomplished conditionally. Thus function can be used for high speed matching unit of dynamic data flow computer. And CARM also can encode matching address sequentially according to therir priority. CARM consists of 8 blocks(CAM cell, Sequential Address Encoder(S.A.E). Reentrant operation. Read/Write control circuit, Data/Mask Register, Sense Amplifier, Encoder. Decoder). Designed DARM can be used in data flow computer, pattern, inspection, table look-up, image processing. The simulation is performed using the QUICKSIM logic simulator and Pspice circuit simulator. Having hierarchical structure, the layout was done using the 3{\;}\mu\textrm{m} n well CMOS technology of the ETRI design rule.

• The Journal of Korea Robotics Society
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• v.13 no.1
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• pp.72-78
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• 2018

This paper proposes a Dual Controller System for Precision Control (DCSPC) for control of the gripper. The DCSPC consists of two subsystems, CDSP (Controller based DSP) and CARM (Controller based ARM processor). The CDSP is developed on a DSP processor and controls the gripping motor and LVDT. In particular, the CARM is implemented using Linux and ARM processor according to recent research related to open-source. The robot for high-precision assembly is divided into the robot control and the gripper control section and controls CARM and CDSP systems respectively. In this paper, we also proposed and measured the performance of communication API. As a result, it is expected to recognize improvements in communication between CARM and the robot controller, and will continue to conduct relevant research among other commercial robot controllers.

• BMB Reports
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• v.39 no.3
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• pp.304-310
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• 2006

Transcription factor NF-E2-related factor 2 (Nrf2) regulates the induction of Phase II detoxifying enzymes and antioxidant enzymes in response to many cancer chemopreventive compounds. In this study, we investigated the role of receptor associated coactivator (RAC3) or steroid receptor coactivator-3 (SRC3) and other nuclear co-regulators including CBP/p300 (CREB-binding protein), CARM1 (Coactivator-associated arginine methyltransferase), PRMT1 (Protein arginine methyl-transferase 1), and p/CAF (p300/CBP-associated factor) in the transcriptional activation of a chimeric Gal4-Nrf2-Luciferase system containing the transactivation domain (TAD) of Nrf2 in HepG2 cells. The results indicated that RAC3 up-regulated the transactivation activity of Gal4-Nrf2-(1-370) in a dose-dependent manner. The enhancement of transactivation domain activity of Gal4-Nrf2-(1-370) by RAC3 was dampened in the presence of dominant negative mutants of RAC3. Next we studied the effects of other nuclear co-regulators including CBP/p300, CARM1, PRMT1 and p/CAF, and the results showed that they had different level of positive effects on this transactivation domain activity of Gal4-Nrf2-(1-370). But importantly, synergistic effects of these co-regulators in the presence of RAC3/SRC3 on the transactivation activity of Gal4-Nrf2-(1-370) were observed. In summary, our present study showed for the first time that the 160 RAC3/SRC3 is involved in the functional transactivation of TAD of Nrf2 and that the other nuclear co-regulators such as CBP/p300, CARM1, PRMT1 and p/CAF can also transcriptionally activate this TAD of Nrf2 and that they could further enhance the transactivation activity mediated by RAC3/SRC3.

• Journal of the Korean Institute of Gas
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• v.16 no.4
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• pp.38-43
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• 2012

The purpose of this study is to carry out the consequence analysis of an accident related to the release of Hydrogen chloride occurred in a petrochemical company in Korea and suggest the measures to prevent similar accidents from happening again. The total amount released through the safety valve of HCl Column was calculated based on the rated capacity of the safety valve, the ideal gas equation and mechanical energy balance, respectively. As a result of the calculation, we found that the amount of HCl released through the safety valve was at least 76.8 kg. Also, we predicted the dispersion concentration at the position of the injured workers(more than 350 m away from the accident location) using simulation programs such as PHAST. The results of ALOHA and K-CARM are 304 ppm and 1,700 ppm respectively. However, PHAST calculation indicated that the concentration is less than l ppm. From these results, we can understand that workers were injured by HCl gas released from the safe valve and the concentration of gas might be less than 1 ppm. Also, it is important for toxic gases such as HCl to be vented safely to the atmosphere after scrubbing.

• Proceedings of the PSK Conference
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• 2002.10a
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• pp.330.1-330.1
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• 2002

Arginine methylation is a common post-translation protein modification in eukaryotic cells. Protein-arginine N-methyltransferase transfer methyl groups from S-adenosyl-L-methionine to the guanidino group of arginine residues. However. The significant of this modification has been questionable. because it occurs rarely and is present at very low abundance. Recently, the discovery of two protein arginine methyltransferase, PRMT1 and CARM1, as cofactors required for responses to muclear Hormone receptors provided an indicationthat arginine methylationhave an important role in transcriptional regulation. (omitted)

• 한국체육학회지인문사회과학편
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• v.55 no.1
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• pp.221-233
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• 2016

The purpose of this study was to prove a development and initial validation of the korean version of coach-athlete relationship maintenance scale that originated from the work of Rhind & Jowett(2012) in pro baseball. The items were then administered to 132 Participants(29 coaches and 103 athletes) completed the questionnaires of the coach-athlete relationship maintenance in First preliminary investigation. Maximum likelihood estimate was used to identify the latent underlying structure. In order to verify the validity of Korean version of coach-athlete relationship maintenance was administered to an independent sample of 273 coaches and athletes. Pro baseball coach-athlete relationship maintenance is consisted of six factors(25 items) with conflict management, motivational, preventative, openness/assurance, support, and social network. SPSS18.0 and AMOS16.0 were used to analyze the exploratory factor analysis, confirmatory factory analysis and internal consistency, test-retest with bootstrapping using of the data in this study. The results of the pro baseball coach-athlete relationship maintenance scale had six factors with 25 items, and each six factor was positively correlated. Overall, this study verified pro baseball coach-athlete relationship maintenance questionnaire. Thus, suggest that path of comparing the differences between the first division and farm team by using the test of the structural model invariance across the groups.