• Journal of Embryo Transfer
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• v.11 no.3
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• pp.259-269
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• 1996

The present study was undertaken to examine the critical effect of $Ca^2$+ concentration on electrostimulation and post-electrostimulation media for electric activation of in vitro matured oocytes of Korean Native Cattle. Oocytes collected from slaughterhouse ovaries were matured in TCM 199 containing FSH, estradiol-17$\beta$ and FBS with granulosa cell monolayer for 24 hours and denuded with hyaluronidase. And then cumulus-free oocytes were submitted to a DC field of 1.0 kV/cm for 60 $\mu$sec in electroporation media(0.28 M mannito' and PBS) with different $Ca^2$+ concentations (0.00, 0.05, 0.10 and 0.15 mM). Stimulated oocytes were stained and examined for pronuclear formation after incuhation in SOF for 12 hours. The rates of pronuclear formation in hovine oocytes electrically stimulated in 0.28 M mannitol with 0.05, 0.10 and 0.15 mM $Ca^2$+(60.3, 82.2 and 75.0%) were significantly higher than without $Ca^2$+(6.3%) at 12 hours after an electric pulse(p<0.005). The activation rates of Korean Native Cattle oocytes stimulated in PBS supplemented with 0.05, 0.10 and 0.15 mM $Ca^2$+(71.0, 75.8 and 75.4%) were significantly higher than without $Ca^2$+(23.5%) after post-stimulation incubation(p<0.005). After incubation of oocytes in SOF with and without $Ca^2$+ following electric stimulation in 0.28 M mannitol with 0.10 mM $Ca^2$+, the rates of pronuclear formation of bovine oocytes in $Ca^2$+-free SOF(85.7%) was significantly higher than in SOF with 1.71 mM $Ca^2$+(62.5%, p<0.05). When oocytes were stimulated in two electrostimulation media supplemented with $Ca^2$+ and incubated in $Ca^2$+-free SOF, there were no significant differences in the rates of pronuclear formation hetween 0.28 M mannitol and PBS. These results indicate that a single electric pulse could induce activation of Korea Native Cattle oocytes in 0.28 M mannitol and PBS supplemented with $Ca^2$+. Furthermore, to improve the activation rates, it was hetter that stimulated oocytes were incubated in $Ca^2$+-free SOF after electric stimulation than in SOF with $Ca^2$+.

• Proceedings of the Korean Society of Broadcast Engineers Conference
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• 2013.11a
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• pp.196-199
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• 2013

제한수신시스템(Conditional Access System, CAS)은 사용자의 조건에 따라 방송에 대한 접근을 제어하는 시스템으로 유료 방송 시스템에서 인가된 사용자만이 해당 프로그램에 접근할 수 있도록 하는 콘텐츠 보안 기술이다. 최근에는 기존의 하드 웨어 기반 CAS가 가지는 이기종 기기간 호환성과 다른 서비스와의 연동 등의 문제를 해결하고자 CAS 모듈을 소프트웨어로 구현하여 네트워크를 통해 설치하는 교환 가능형 제한수신시스템(eXchangeable CAS, 이하 XCAS) 방식을 도입하여 활발하게 서비스를 제공하고 있다. XCAS는 기존 CableCard방식이 가지는 CAS의 보안성과 분리교환 원칙을 유지하면서도 다양한 단말에 쉽게 설치하여 서비스가 가능하다는 점에서 큰 장점을 가지고 있지만, 방송수신만을 목적으로 하는 DTV(Digtal Television)에 대해 적용하기가 어렵다는 문제점이 존재하였다. 본 논문에서는 케이블방송환경에서 DTV를 통한 유료방송서 비스 제공을 위한 DTV 인증 및 XCAS 서비스에 필요한 SM(Secure Module) 모듈을 다운로드하기 위한 기법을 제안한다.

• Journal of the Korean Ceramic Society
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• v.15 no.4
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• pp.199-204
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• 1978

In the present investigation, refined alumina obtained from alunite locally available was used as a aluminous source to synthesize monocalcium aluminate $(CA)_3$ the major mineral constituent of alumina cement. The influence of $SiO_2$ and $Fe_2O_3$ contents on the formation of monocalcium aluminate was studied by X-ray diffraction analysis mainly. About 0.8-1.0 of $Al_2O_3/C_3O$ mol ratio and less than 4 percent of $SiO_2$ were desirable for the effective formation of CA. The small amount of alkali and sulphur contents contained in refined alumina from alunite as the impurities were affected to form $C_4A_3S$ and $C_3S_2$, disadvantageous compounds for the alumina cement, therefore the impurities should be restricted in minimum content as possible.

• The Korean Journal of Ecology
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• v.28 no.3
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• pp.169-180
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• 2005

Nitrogen dioxide concentration was being grown ca. 8% per year although sulphur dioxide concentration was being reduced ca. 10% per year in Seoul from 1996 to 2003. The rainwater was heavily acidic with pH 4.19 to 4.68 in Seoul during 1980 to 1991. Acidity score on the basis of the total anion concentration of rainwater follows the higher in sequence with Seoul(Kwanaksan)(341.5 ${\mu}equiv{\cdot}L^{-1}$), Gwangneung(199.2 ${\mu}equiv{\cdot}L^{-1}$) and whole Korea mean(10.3 ${\mu}equiv{\cdot}L^{-1}$). Rainwaters from 38 sampling sites of 16 countries in the world, on the basis of the acidity score, are classified and designated as five acid rain grades(ARG): the least score is founded in Tana Rata(16.2 ${\mu}equiv{\cdot}L^{-1}$), Malaysia where has the best rain but the greatest founded in Chongquing(541.1 ${\mu}equiv{\cdot}L^{-1}$), China where has the worst rain in the world. Even though acidic deposition decrease with time in the world generally, it is expected to increase in Asia, Africa and Mid-South America.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.15
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• pp.6255-6259
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• 2015

Purpose: To evaluate the prognostic value of alkaline phosphatase (ALP) and gamma-glutamyltransferase (GGT) in gallbladder cancer (GBC). Materials and Methods: Serum ALP and GGT levels and clinicopathological parameters were retrospectively evaluated in 199 GBC patients. Receiver operating characteristic (ROC) curve analysis was performed to determine the cut-off values of ALP and GGT. Then, associations with overall survival were assessed by multivariate analysis. Based on the significant factors, a prognostic score model was established. Results: By ROC curve analysis, $ALP{\geq}210U/L$ and $GGT{\geq}43U/L$ were considered elevated. Overall survival for patients with elevated ALP and GGT was significantly worse than for patients within the normal range. Multivariate analysis showed that the elevated ALP, GGT and tumor stage were independent prognostic factors. Giving each positive factor a score of 1, we established a preoperative prognostic score model. Varied outcomes would be significantly distinguished by the different score groups. By further ROC curve analysis, the simple score showed great superiority compared with the widely used TNM staging, each of the ALP or GGT alone, or traditional tumor markers such as CEA, AFP, CA125 and CA199. Conclusions: Elevated ALP and GGT levels were risk predictors in GBC patients. Our prognostic model provides infomration on varied outcomes of patients from different score groups.

• Transactions on Electrical and Electronic Materials
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• v.5 no.5
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• pp.199-203
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• 2004

Superconducting B $i_2$S $r_2$C $a_{n-l}$C $u_{n}$ $O_{x}$(n=2～4) thin films were prepared by single target DC-magnetron sputtering. And, that was compared with the B $i_2$S $r_2$C $a_{n-l}$C $u_{n}$ $O_{x}$(n=1～3) thin film fabricated by using the ion beam sputtering. Phase intergrowth among n=2-3, 3-4 and 4-5 phases was observed. The molar fraction of each phase in the mixed crystal of the deposited films was determined by x-ray diffraction analyses and investigated as a function of $O_2$ gas pressure during sputtering. We investigated the changes of the superconducting properties by molar fraction of each phase. Also, the thin film surface observation was carried out by atomic force microscope. The images show the average particle size decreases, and the distribution density of particles on the film surface was to increase with lower gas pressures. The fabrication conditions for selective growth of the single n=2, 3 and 4 phases in BiSrC $a_{n-l}$C $u_{n}$ $O_{x}$(n=2～4) thin film are discussed.e discussed.ussed.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.30 no.4
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• pp.199-203
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• 2017

$ZrO_2/PSS$ thin film with a high refractive index was fabricated on a glass substrate by a layer-by-layer self-assembly method. The surface morphology and thickness of the fabricated $ZrO_2/PSS$ thin films were measured as a function of the number of $(ZrO_2/PSS)n$. As the number of $(ZrO_2/PSS)n$ increased from n = 5 to n = 20, RMS roughness decreased from 29.01 nm to 8.368 nm. The $ZrO_2$ thin films exhibited high transmittance of 85% or more; and the 15-bilayer thin film exhibited the highest transmittance among the samples. The transmittance of the fabricated $(ZrO_2/PSS)_{15}$ thin film was ca. 90.8% in the visible range. The refractive index of the glass substrate coated by a $(ZrO_2/PSS)_{15}$ thin film with a thickness of 160 nm increased from ca. 1.52 to 1.74 at the 632 nm wavelength.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.9
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• pp.4041-4044
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• 2015

Background: To verify whether serum tumor abnormal protein (TAP) would correlate with the responsiveness of palliative chemotherapy in patients with advanced gastric cancer, and the variation of conventional serum tumor markers e.g., carcinoembryonic antigen (CEA), antigen 125 (CA125),carbohydrate antigen19-9 (CA19-9) of adjuvant chemotherapy in patients with early gastric cancer. Materials and Methods: Patients with histologically confirmed gastric cancer and treated with chemotherapy were enrolled into this study. TAP values of these patients were determined by detecting abnormal sugar chain glycoprotein in serum, combined with the area of agglomerated particles. For patients with advanced gastric cancer, responsiveness of palliative chemotherapy was compared with variation of TAP and the relation between variation of TAP and tumor markers in patients with early gastric cancer was analyzed. Results: Totally 82 gastric cancer patients were enrolled into this study. The value of TAP is more closely related to responsiveness of palliative chemotherapy for patients with advanced gastric cancer. The correlation between TAP and responsiveness to palliative chemotherapy is stronger than the correlation between several conventional serum tumor markers (CEA, CA125 and CA199). The variation of TAP was also positively correlated with the trend of CA125 in adjuvant chemotherapy. Conclusions: TAP is sensitive in monitoring the responsiveness to palliative chemotherapy in patients with advanced gastric cancer. But this result should be confirmed by randomized clinical trials for patients with gastric cancer.

• Animal cells and systems
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• v.4 no.1
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• pp.51-55
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• 2000

Mass loss and changes of mineral nutrients during the decomposition of mushrooms, Russula alboareolata and Lactarius violascens, were investigated for 7d from June 29 to July 5 in 1999 in an oak stand in Kongju, Korea. At 7d after installation of litterbags, the remaining mass of R. alboareolata and L. violaxcens was 9.4% and 25.9%, respectively. The mass loss rate of R. alboareolata was significantly higher than that of L. violascens. Concentration of N, P, K, Ca and Mg of R. alboareolata and L. violascens were 37.7 mg/g, 0.97mg/g, 38.25 mg/g, 0.04 mg/g, and 0.75 mg/g for R. alboareolata and 45.7 mg/g, 1.31mg/g, 24.0 mg/g, 0.06 mg/g, and 0.80 mg/g for L. violascens, respectively. Concentrations of nutrients in R. alboareolata and L. violascens were much higher than those in the surrounding leaf litter. N, P, Ca and Mg concentrations in the decomposing mushroom tissue were higher during the experimental period in both species than initial concentrations. Potassium increased during the first 3 d and then decreased in both species. Potassium contents in the mushroom were much greater than those of Ca and Mg. Except for Ca, there was no immobilization period in all the nutrients during decomposition. At 7 d after installation of litterbags, the remaining N, P, K, Ca and Mg of R. alboareolata and L. violascens were 9.8%, 8.9%, 2.7%, 47.7%, and 14.8% of the initial contents for R. alboareolata and 28.2%,30.5%, 19.6%, 199.9%, and 02.1% for L. violascens, respectively. Nutrients could be relocated spatially during the formation and decomposition of the Basidiomycetes fruiting body.

• Journal of Breast Cancer
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• v.21 no.4
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• pp.382-390
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• 2018

Purpose: PIK3CA mutation is considered to be a possible cause for resistance to neoadjuvant chemotherapy (NAC) in human epidermal growth factor receptor 2 (HER2)-positive breast cancer. We investigated the association between PIK3CA mutations and the outcome of NAC in HER2-positive breast cancers. Methods: A total of 100 HER2-positive breast cancer patients who had undergone NAC and surgery between 2004 and 2016 were examined. Mutation status was sequentially assessed in pre-NAC, post-NAC, and recurrent specimens taken from these patients. Results: PIK3CA mutations were identified in the sequential specimens of 17 patients (17.0%). These 17 patients experienced shorter disease-free survival (DFS) than the rest of the patients (58.3 months vs. 119.3 months, p=0.020); however, there was no significant difference in pathologic complete response (pCR) and overall survival (OS) (pCR, 17.6% vs. 33.7%, p=0.191; OS, 84.5 months vs. 118.0 months, p=0.984). While there was no difference in pCR between the wild-type and mutant PIK3CA groups in pre-NAC specimens (25.0% vs. 31.8%, p=0.199), PIK3CA mutations correlated with lower pCR in postNAC specimens (0.0% vs. 24.3%, p<0.001). Multivariate analysis revealed significantly worse DFS in the mutant PIK3CA group than in the wild-type group (hazard ratio, 3.540; 95% confidence interval, 1.001-12.589; p=0.050). Moreover, the DFS curves of the change of PIK3CA mutation status in sequential specimens were significantly different (p=0.016). Conclusion: PIK3CA mutation in HER2-positive breast cancer was correlated with a lower pCR rate and shorter DFS. These results suggest that PIK3CA mutation is a prognostic marker for NAC in HER2-positive breast cancer, especially in post-NAC specimens.