• BMB Reports
• /
• v.29 no.6
• /
• pp.545-548
• /
• 1996

CA(1-8)ME(1-12), the CA-ME hybrid peptide of the amino terminal segments of cecropin A (CA) and melittin (ME), has been reported to have a broad spectrum and improved potency without a hemolytic property. In order to obtain new synthetic peptides with powerful antibacterial activity without hemolytic activity, several hybrid peptides were designed from the sequences of CA, ME, magainin 2, bombinin and lactoferricin. All hybrid peptides were constructed to form an amphipathically basic-flexible-hydrophobic structure and synthesized by the solid phase method. Their hemolytic activities against human red blood cells and antibacterial activities against both Gram-positive and Gram-negative bacteria were detennined. CA(1-8)MA(1-12), CA(1-8)BO(1-12), MA(10-17)ME(1-12) and LF(20-29)ME(1-12) showed comparable activities with broad spectra against both Gram-positive and Gram-negative bacteria relative to CA(1-8)ME(1-12) but without hemolytic properties. These hybrid peptides, therefore, could be useful as model peptides to design a novel peptide with improved antibacterial activity and study on structure-activity relationships of antimicrobial peptides.

• KSCE Journal of Civil and Environmental Engineering Research
• /
• v.31 no.6D
• /
• pp.785-791
• /
• 2011

Two-lane and two-way traffic flow shows various dynamic relationships according to the behaviors of low-speed vehicle and overtaking. And it is essential to develop a vehicle model which simultaneously explains the behaviors of low-speed vehicle and overtaking using opposite lane in order to microscopically analyze various two-lane and two-way traffic flows by traffic flow simulation. In Korea, some studies for car-following and lane-changing models for freeway or signalized road have been reported, but few researches for the development of vehicle model for two-lane and two-way highway have been done. Hence, a microscopic two-lane and two-way vehicle model was, in this study, developed with the consideration of overtaking process and is based on CA (Cellular Automata) which is one of discrete time-space models. The developed model is parallel combined with an adjusted CA car-following model and an overtaking model. The results of experimental simulation showed that the car-following model explained the various macroscopic relationships of traffic flow and overtaking model reasonably generated the various behaviors of macroscopic traffic flows under the conditions of both opposite traffic flow and stochastic parameter to consider overtaking. The vehicle model presented in this study is expected to be used for the simulation of more various two-lane, two-way traffic flows.

• Journal of Korean Society of Rural Planning
• /
• v.14 no.2
• /
• pp.55-64
• /
• 2008

This study aims to analyze the sensitivity of WSM(weighted scenario method)-AHP method according to variation of nonlinear exponent for accessibility criteria, which are used to make urbanization potential maps with the optimal weighting value for multiple criteria in grid-based GIS technique. Besides this study tried to develop WSM-AHP2 which is simplified by using rank of the potential value for each scenario. The two methods were applied to the test area, Suwon city located south area of Seoul, with time series land-use maps of 1986 and 1996. The evaluation system of urbanization potential have 7 criteria including 6 accessibility criteria. The results of WSM-AHP2, the optimal weighting values and their corresponding potential maps, have almost similar with those of WSM-AHP. In the application of CA(cellular automata) model for expansion of urbanized area using the three potential maps by WSM-AHP, WSM-AHP2, and specialists's AHP evaluation, it also showed that the accuracy of simulation for actual urban area is the highest in the potential map of WSM-AHP, followed by WSM-AHP2 and specialists's AHP evaluation. From the results of this study, WSM-AHP and simplified WSM-AHP2 will be used to generate the optimal potential maps for land-use planning in urban fringe area.

• The Journal of Advanced Prosthodontics
• /
• v.9 no.6
• /
• pp.482-485
• /
• 2017

PURPOSE. This laboratory study aimed to investigate the effect of doping an acrylic denture base resin material with nanoparticles of ZnO, CaO, and $TiO_2$ on biofilm formation. MATERIALS AND METHODS. Standardized specimens of a commercially available cold-curing acrylic denture base resin material were doped with 0.1, 0.2, 0.4, or 0.8 wt% commercially available ZnO, CaO, and $TiO_2$ nanopowder. Energy dispersive X-ray spectroscopy (EDX) was used to identify the availability of the nanoparticles on the surface of the modified specimens. Surface roughness was determined by employing a profilometric approach; biofilm formation was simulated using a monospecies Candida albicans biofilm model and a multispecies biofilm model including C. albicans, Actinomyces naeslundii, and Streptococcus gordonii. Relative viable biomass was determined after 20 hours and 44 hours using a MTT-based approach. RESULTS. No statistically significant disparities were identified among the various materials regarding surface roughness and relative viable biomass. CONCLUSION. The results indicate that doping denture base resin materials with commercially available ZnO, CaO, or $TiO_2$ nanopowders do not inhibit biofilm formation on their surface. Further studies might address the impact of varying particle sizes as well as increasing the fraction of nanoparticles mixed into the acrylic resin matrix.

• Journal of the Mineralogical Society of Korea
• /
• v.11 no.2
• /
• pp.106-116
• /
• 1998

The adsorption of Cs-137 and Sr-90 onto kaolinite in prescence of major groundwater cations (Ca2+, K+, Na+) with different concentrations was simulated by using triple-layer surface complexation model (TL-SCM). The site density (8.73 sites/nm2) of kaolinite used for TL-SCM was calculated from it's CEC and specific surface area. TL-SCM modeling results indicate that concentrations dependence on 137Cs and 90Sr adsorption onto kaolinite as a function of pH is best modeled as an outer-sphere surface reaction. This suggests that Cs+ and Sr2+ are adsorbed at the $\beta$-layer in kaolinite-water interface where the electrolytes, Nacl, KCl and CaCl2, bind. However, TL-SCM results on Sr adsorption show a discrepancy between batch data and fitting data in alkaline condition. This may be due to precipitation of SrCO3 and complexation such as SrOH+. Intrinsic reaction constants of ions obtained from model fit are as follows: Kintcs=10-2.10, KintSr=10-2.30, KintK=10-2.80, KintCa=10-3.10 and KintNa=10-3.32. The results are in the agreement with competition order among groundwater ions (K+>Ca2+>Na+) and sorption reference of nuclides (Cs-137>Sr-90) at kaolinite-water interface showed in batch test.

• Journal of the Korea Society of Computer and Information
• /
• v.17 no.5
• /
• pp.71-80
• /
• 2012

In this paper, applications that require low-speed data rate on personal area network to operate the Land Warrior system is proposed. These applications can refer to WSN(wireless sensor network) technology. However, this technology is not suitable to support various data transmission rate. A suitable CSMA/CA algorithm for Land Warrior System in order to solve these existing system problems is proposed. The proposed algorithm is designed to be variable CSMA/CA algorithm parameter, depending on data rate. For the evaluation of Land Warrior system model and CSMA/CA algorithm, we used Castalia. As a result of the simulation, it is found that the proposed system model can not only relieve loads of data processing, but also probability of collision was decreased.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.36 no.3A
• /
• pp.267-277
• /
• 2011

Like other wireless network protocols, IEEE 802.15.4 adopts CA (Collision Avoidance)algorithm to avoid the early collision of a new packet by randomizing its first transmission time rather than its immediate delivery. The traditional CA scheme of IEEE 802.15.4, however, selects the random access time from the predetermined range without considering the degree of current congestion. It probably causes either a long delay to settle in the suitable range for the current network load or frequent clashes especially during the long lasting heavy traffic period. This paper proposes an ACA(Adaptive Collision Avoidance) algorithm adapting the initial backoff range to the undergoing collision variations. It also introduces a mathematical model to predict the performance of ACA algorithm added to IEEE 802.15.4. With only small deviations from corresponding simulations, our analytical model shows that ACA technique can improve the throughput of IEEE 802.15.4 by up-to 41% maximally in addition to shortening the packet delays.

• The Korean Journal of Physiology and Pharmacology
• /
• v.6 no.4
• /
• pp.207-214
• /
• 2002

The present study was designed to clarify whether tacrine affects the release of catecholamines (CA) from the isolated perfused model of rat adrenal gland or not and to elucidate the mechanism of its action. Tacrine $(3{\times}10^{-5}{\sim}3{\times}10^{-4}\;M)$ perfused into an adrenal vein for 60 min inhibited CA secretory responses evoked by ACh $(5.32{\times}10^{-3}\;M),$ DMPP (a selective neuronal nicotinic agonist, $10^{-4}$ M for 2 min) and McN-A-343 (a selective muscarinic M1-agonist, $10^{-4}$ M for 2 min) in relatively dose- and time- dependent manners. However, tacrine failed to affect CA secretion by high $K^+\;(5.6{\times}10^{-2}\;M).$ Tacrine itself at concentrations used in the present experiments did not also affect spontaneous CA output. Furthermore, in the presence of tacrine $(10^{-4}\;M),$ CA secretory responses evoked by Bay-K-8644 (an activator of L-type $Ca^{2+}$ channels, $10^{-4}\;M),$ but not by cyclopiazonic acid (an inhibitor of cytoplasmic $Ca^{2+}-ATPase,\;10^{-4}\;M),$ was relatively time-dependently attenuated. Also, physostigmine $10^{-4}\;M),$ given into the adrenal gland for 60 min, depressed CA secretory responses evoked by ACh, McN-A-343 and DMPP while did not affect that evoked by high $K^+.$ Collectively, these results obtained from the present study demonstrate that tacrine greatly inhibits CA secretion from the perfused rat adrenal gland evoked by stimulation of cholinergic (both nicotinic and muscarinic) receptors, but does fail to affect that by direct membrane-depolarization. It is suggested that this inhibitory effect of tacrine may be exerted by blocking both the calcium influx into the rat adrenal medullary chromaffin cells without $Ca^{2+}$ release from the cytoplasmic calcium store, that is relevant to the cholinergic blockade. Also, the mode of action between tacrine and physostigmine in rat adrenomedullary CA secretion seems to be similar.

• KSBB Journal
• /
• v.26 no.1
• /
• pp.13-18
• /
• 2011

In this study, precipitation process was developed for the recovery of the lactic acid from calcium lactate fermentation broth. Calcium lactate yield was improved by decreasing the solubility of calcium lactate through the addition of ethanol (25%, v/v) as a co-precipitant. The optimal lime type, lime concentration, stirrer speed, precipitation time, temperature, and solvent amount for $Ca(LA)_2$ precipitation were CaO, 0.0175 g/mL, 220 rpm, 24 h, $5^{\circ}C$, ethanol 25% (v/v), respectively. Lactic acid was easily and efficiently recovered from precipitated $Ca(LA)_2$ by adding sulfuric acid ($Ca(LA)_2/H_2SO_4$ molar ratio=1:1). In the model solution of organic acids and fermentation broth, the overall yields of recovered lactic acid were 62% and 55%, respectively, under the aforementioned optimal conditions.

• Biomolecules & Therapeutics
• /
• v.12 no.3
• /
• pp.165-174
• /
• 2004

The aim of the present study was to investigate the effect of FCCP (carbonyl cyanide p-trifluoromethoxyphenyIhydrazone), which is a potent mitochondrial uncoupler, on secretion of catecholamines (CA) from the perfused model of the rat adrenal gland and to establish the mechanism of its action. The perfusion of FCCP (3 ${\times}$ $10^{-5}$ M) into an adrenal vein of for 90 min resulted in great increases in CA secretions. Tachyphylaxis to CA-releasing effect of FCCP was not observed by repeated perfusion of it. The CA-releasing effects of FCCP were depressed by pre-treatment with pirenzepine, chlorisondamine, nicardipine, TMB-8, and the perfusion of EGTA plus $Ca^{2+}$-free medium. In the presence of FCCP (3 ${\times}$ $10^{-5}$ M), the CA secretory responses induced by Ach (5.32 ${\times}$ $10^{-3}$ M), and DMPP ($10^{-4}$ M) were significantly enhanced. Furthermore, the perfusion of CCCP (3 ${\times}$ $10^{-5}$ M), a similar mitochondrial uncoupler, into an adrenal vein for 90 min also caused an increased response in CA secretion. Taken together these experimental results indicate that FCCP causes the CA secretion the perfused rat adrenal medulla in a calcium-dependent fashion. It is suggested that this facilitatory effects of FCCP may be mediated by cholinergic receptor stimulation, which is relevant to both stimulation of the $Ca^{2+}$ influx and $Ca^{2+}$ release from cytoplasmic $Ca^{2+}$ stores.