• Title/Summary/Keyword: C7

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Inhibition of Listeria monocytogenes by Low Concentrations of Ethanol (저농도의 Ethanol에 의한 Listeria monocytogenes의 증식억제)

  • 박찬성;김미림
    • Korean journal of food and cookery science
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    • v.11 no.4
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    • pp.379-385
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    • 1995
  • The effect of low concentrations of ethanol (3-7%, v/v) in tryptic soy broth (TSB) as an antibacterial agent against Listeria monocytogenes was tested at -20, 5, 35, 45, 50 and 55$^{\circ}C$. Increasing concentrations of ethanol progressively inhibited initial growth of L. monocytogenes at 35$^{\circ}C$. Growth occured at 5% ethanol, but only after a prolonged lag period. The number of viable cells of L. monocytogenes declined during incubation at 7% ethanol. TSB containing 3-7% ethanol was inoculated with 10$\^$5/-10$\^$6/ cells/$m\ell$ or L. monocytogenes and incubated at low temperatures (5$^{\circ}C$, -20$^{\circ}C$). In the presence of 3% of ethanol at 5 or -20$^{\circ}C$, bacterial growth was inhibited more than 90% of control cells. TSB containing 3-7% ethanol was inoculated with 10$\^$6/-10$\^$7/ cells/$m\ell$ of L. monocytogenes and incubated at high temperatures (45$^{\circ}C$, 50$^{\circ}C$, 55$^{\circ}C$). Decrease in viability of the cells incubated at 45 or 50$^{\circ}C$ was slow and the survival of L. monocytogenes was not affected so much in the presence of 3% of ethanol. The viability of L. monocytogenes was decreased with increasing concentration of ethanol and temperature. Decimal reduction times (D-values) based on tryptic soy agar plates at 55$^{\circ}C$ were 20.1, 12.6, 7.4 and 4.2 min in 0, 3, 5 and 7% ethanol, respectively.

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A Study for Oxidation Reaction of Alcohols with Cr (Ⅵ)-Isoquinoline Compound (Ⅰ) (Cr (Ⅵ)-Isoquinoline 화합물에 의한 알코올들의 산화반응에 관한 연구 (Ⅰ))

  • Yang, Jeong Seong;Park, Yeong Jo;Baek, Hyeong Cheol
    • Journal of the Korean Chemical Society
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    • v.34 no.6
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    • pp.534-538
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    • 1990
  • Chromium(Ⅵ) compound, [$C_9H_7$NH]$_2Cr_2O_7$ has been prepared by the reaction of isoquinoline with chromium trioxide under the presence of water. [$C_9H_7$NH]$_2Cr_2O_7$ is nonhydroscopic and dissolved in water very well. Structure of [$C_9H_7$NH]$_2Cr_2O_7$ is identified by FTIR spectra and elemental analysis data. The ability of [$C_9H_7$NH]$_2Cr_2O_7$ for the oxidation of alcohols were examined in methylene chloride. [$C_9H_7$NH]$_2Cr_2O_7$ was found as efficient oxidizing agent for the conversion of allyl, primary and secondary alcohols to the corresponding aldehydes or ketones.

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Effects of Preheating and Ascorbate on Heat Resistance of Escherichia coli O157:H7 (Escherichia coli O157:H7 균주의 열저항성에 미치는 예비열처리 및 Ascorbate의 효과)

  • 권오진;김덕진;김순희;변명우
    • Journal of Food Hygiene and Safety
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    • v.12 no.4
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    • pp.304-309
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    • 1997
  • A study was undertaken to determine the thermal inactivation of Escherichia coli O157:H7 as influenced by the effects of temperature, time, suspension medium and ascorbate. Tryptic soy broth was more heat resistant than pfosphate buffer (pH 7.1), with D values of 1.52~1.68 min at 6$0^{\circ}C$ and 1.51~1.63 min at 7$0^{\circ}C$ compared with 1.52~1.65 min at 6$0^{\circ}C$ and 1.26~1.61 min at 7$0^{\circ}C$ for phosphate buffer as suspension medium. E. coli O157:H7 was completely inhibited within 30 min when small inoculum (106 CFU/$m\ell$) was heated at 7$0^{\circ}C$. When E. coli O157:H7 was preheated at 48$^{\circ}C$ for 60 min in phosphate buffer before heating, D values were 1.28~1.60 min at 6$0^{\circ}C$, and 1.13~1.56 min at 7$0^{\circ}C$, showing that preheating increases the heat resistance of the strain. Phosphate buffer containing ascorbate (0.001 M) was enhanced the thermal inactivation of the strain when inoculated as large inoculum (109 CFU/$m\ell$), while ascorbic acid was no effect at low cell concentrations (109 CFU/$m\ell$).

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To Study About Distance from Skin to Cervical Epidural Space in Korean Adults (한국인 성인에서 피부로부터 경추경막외강까지의 깊이에 대한 임상연구)

  • Kwack, Ki-Jong
    • The Korean Journal of Pain
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    • v.10 no.1
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    • pp.69-72
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    • 1997
  • Background : This study was designed to determine if cervical epidural depth has any correlation with age, height, weight, ponderal index and neck clrcunference. Methods : Data was obtained from 102 patients successfully anesthetized with cervical epidural block. Patients were categorized into 4 groups: male C6-7, male C7-T1, female C6-7, female C7-T1 then statistically compared and analyzed. Results : The mean and standard deviation of epidural depth of male C6-7, male C&-T1, female C6-7, female C7-T1 groups were respectively as follows: 41.1+/-6.0, 47.1+/-5.6, 37.9+/-6.0, 46.4+/-5.6 mm. The results showed cervical epidural depth is well correlated with body weight and ponderal index; moderately correlated with neck circumference: slightly correlated with age; no correlation with height.

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Transition of 12CaO·7Al2O3 electrical insulator to the permanent semiconductor using via thermo-chemical reduction treatment (열 화학적 환원 처리를 이용한 절연체 12CaO·7Al2O3의 전도체로의 전환)

  • Chung, Jun-Ho;Eun, Jong-Won;Oh, Dong-Keun;Kim, Kwang-Jin;Hong, Tae-Ui;Jeong, Seong-Min;Choe, Bong-Geun;Shim, Kwang-Bo
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.20 no.4
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    • pp.178-184
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    • 2010
  • The $12CaO{\cdot}7Al_2O_3$(C12A7) powders were successfully synthesized using combustion method with microwave-assistant and C12A7:H were fabricated by post-annealed process in Ar/H atmosphere. X-ray diffraction patterns and TGDSC were used for investigating to the precursors of crystalline and reaction depending on temperature. C12A7:H that was treated post-annealed process were investigated TG-MS and Hall-measurement for confirming H ions doping and checking electrical resistivity of C12A7:H. H ion substituted to $O^{2-}$ ions in the C12A7 cages were confirmed at $289.5^{\circ}C$ by TG-MS and C12A7:H calcined at $1000^{\circ}C$ in Ar/H=8:2 atmosphere for 8~10 h has low electrical resistivity about $10^2{\Omega}{\cdot}cm$ at room temperature.

Comparison of ultrasound-guided stellate ganglion block at 6th and 7th cervical vertebrae using the lateral paracarotid out-of-plane approach for sympathetic blockade in the upper extremity

  • Baek, Jongyoon;Kim, Bum Soo;Yu, Hwarim;Kim, Hyuckgoo;Lim, Chaeseok;Song, Sun Ok
    • Journal of Yeungnam Medical Science
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    • v.35 no.2
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    • pp.199-204
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    • 2018
  • Background: The authors have performed ultrasound-guided stellate ganglion block (SGB) in our clinic using a lateral paracarotid approach at the level of the 6th cervical vertebra (C6). Although SGB at C6 is a convenient and safe method, there are ongoing concerns about the weak effect of sympathetic blockade in the ipsilateral upper extremity. Therefore, ultrasound-guided SGB was attempted using a lateral paracarotid approach at the level of the 7th cervical vertebra (C7). This prospective study aimed to compare changes in skin temperature after SGB was performed at C6 and C7, and to introduce a lateral paracarotid approach for SGB. Methods: Thirty patients underwent SGB twice: once at C6 and once at C7. For every SGB, the skin temperature of the patient's hypothenar area was measured for 15 min at 1-min intervals. Skin temperatures before and after SGB and side effects were compared between C6 and C7 groups. Results: The temperature of the upper extremity increased after SGB was performed at C6 and C7. There were significant differences between mean pre-SGB and the largest increases in post-SGB temperatures ($0.50{\pm}0.38^{\circ}C$ and $1.41{\pm}0.68^{\circ}C$ at C6 and C7, respectively; p<0.05). Significantly increased post-SGB temperatures (difference > $1^{\circ}C$) were found in 5/30 (16.7%) and 24/30 (80%) cases for C6 and C7, respectively (p<0.05). There were no significant differences in side effects between SGB performed at C6 or C7 (p>0.05). Conclusion: The lateral paracarotid approach using out-of-plane needle insertion for ultrasound-guided SGB performed at C7 was feasible and more effective at elevating skin temperature in the upper extremity than SGB at C6.

Isolation and Charaterization of Microorganism Producing Cephalosporin C Acylase (Cephalosporin C Acylase 생산균주의 분리 및 특성)

  • Park, Yong-Chjun;Kim, Ook-Hyun;Lim, Jai-Yun;Kim, Young-Chang
    • Microbiology and Biotechnology Letters
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    • v.23 no.5
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    • pp.559-564
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    • 1995
  • Twenty microbial strains producing the acylase were isolated from soil by using Micrococcus luteus ATCC 9341 as an indicator strain, using either D-($\alpha $)-phenylglycine methylester and 7-aminocephalosporanic acid (7-ACA) or glutaric acid dimethylester and 7-ACA as substrates. Among the isolates, only one strain was turned out to be the 7-ACA producer from either cephalosporin C or glutaryl 7-ACA as the substrates by using the overlay of 7-ACA sensitive strain (SS5). 7-ACA produced from cephalosporin C by an isolate (APS20) was detected by high performance liquid chromatography. The isolated strain (APS20) was identified to Bacillus macerans on the basis of cellular fatty acid profile by gas chromatography. Bacillus macerans APS20 had no $\beta $-lacta-mase activity on cephalosporin C, and that is very important for the enzymatic production process of 7-ACA. However, this strain was resistant up to 100 $\mu $g/ml of cephalosporin C.

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Antimicrobial and Antioxidant Effects of Functional Healthy Drinks from Some Medicinal Herbs and Coffee Mixture (약용식물과 커피 혼합물로부터 기능성 건강음료의 항균 및 항산화 효과)

  • Kim, In Hae;Kim, JinKyun;Lee, Jae Hwa
    • Journal of Life Science
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    • v.26 no.11
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    • pp.1225-1231
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    • 2016
  • In this study, our investigated the antioxidant activities and compared other bioassay including anti-microbial, antifungal activities and total polyphenol contents of functional healthy drinks from some medicinal herbs and coffee mixture. The kind of medicinal herbs, chaga mushroom (Inonatus obliqurs), moringa (Moringa Oleifera), gravila (Anona muricata), mulberry (Morus alba), Dioscoreaceae (Dioscorea quinquelaba), Berberidaceae (Epimedii Herba), Asteraceae (Artemisia capillaries) and siberian ginseng (Acanthopanax senticous,). The functional healthy drinks, named C1, C2, C3, C4, C5, C6 and C7 were summered in Table 1. The in vitro antimicrobial activity was examined against Gram positive bacteria, Gram negative bacteria and a fungus. The functional healthy drinks were broad spectrum of anti-microbial activity without antifungal activity against Candida albicans KCTC7965. In particularly, the C7 showed strong activity against Methicillin Resistant Staphylococcus aureus CCARM3089, CCARM 3115 and CCARM3561. And, the C7 showed 88% of free radical scavenging effect on 0.5 mg/ml using 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. Functional healthy drink C7 was mulberry extracts from Morus alba, chaga mushroom from Inonatus obliqurs and moringa from Moringa olifera in additionally coffee extracts. Its results confirm that the potential use of mulberry extracts as a good source of antibacterial compounds or as a health promoting food and health drinks.

Isolation and Identification of Mucor mueedo C-7 for Producing The Milk-clotting Enzyme (응유효소를 생산하는 Mucor mucedo C-7의 분리 및 동정)

  • 조재민;이웅수;김교창
    • Microbiology and Biotechnology Letters
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    • v.18 no.5
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    • pp.449-453
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    • 1990
  • This study was attempted to obtain the efficient milk-clotting enzyme from microorganisms as a rennet subtitute. Fungi which showed the formation ability of the milk-clotting enzyme were selected out from samples of soil hay and wastes etc. Among these isolated fungi, strain no. C-7 which had presented higher value in the ratio of milk-clotting activity to proteolytic activity was selected. The hyphae of this strain was white to gray and no septa. A single sporangiophore which stand erectly above growing hyphae was monomucor type without branching. A globose sporangium was developed at the tip of each sporangiophore. The suitable temperature and pH for the growth of no. C-7 was 20-$30^{\circ}C$ and pH 3.0-8.0 respectively. These morphological and physiological characteristics implied that strain no. C-7 was Mueor mucedo.

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Studies on Wax Esters in Marine Animals(1) -Lipid Composition of Mullet Roe Oil- (수산동물(水産動物)의 Wax Ester에 관(關)한 연구(연구) (1) -숭어난유(卵油)의 지질조성(脂質組成)에 관(關)하여-)

  • Joh, Yong-Kea;Koh, Kwang-Bae
    • Korean Journal of Food Science and Technology
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    • v.10 no.4
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    • pp.409-414
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    • 1978
  • The total amount of lipid content in the mullet roe is 20.5%, and iodine value and unsaponifiable matters content are 118 and 38.7%, respectively. The lipid composition of the muscle, roe and liver of the mullet, Mugil cephalus, shows differences. Triglyceride, wax esters, and free fatty acids are mainly contained in the lipids of the muscle, roe and liver, respectively. The mullet roe lipids are mainly composed of 59.1% of wax esters with a trace of sterol esters, 26.9% of polar lipids with pigments, 9.0% of triglyceride plus a trace of free fatty alcohols and fatty acids, and 3.0% of sterol contaminated with a trace of fatty alcohols. The major fatty acids of wax esters are C16 : 0, 47.5%, C18 : 1, 23.0%, C16 : 3, 6.5%, C20 : 5, 4.0%, those of triglyceride are C16 : 1, 25.1%, C18: 1, 16.7%, C16 : 0, 16.3%, C22 : 1, 7.9%, C18 : 0, 5.5%, C22 : 6, 4.4%, and those of polar lipids are C16 : 0, 35.0%, C18 : 1, 24.7%, C16 : 1, 6.1%, C20 : 5, 5.3%, C22 : 6, 4.2%. The major alcohols of wax esters are 51.0% of cetyl alcohol, 18.2% of palmitoleyl alcohol, and 10.7% of oleyl alcohol, and considerable amounts of odd-numbered alcohols such as C15 : 0, C15 : 1, C17 : 0, C17 : 1 and C19 : 1 are also found.

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