• Korean Journal of Food Science and Technology
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• v.25 no.5
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• pp.576-581
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• 1993

Polygalacturonase(PG) was extracted from Chinese cabbage and partially purified by ammonium sulfate fractionation and ion-exchange chromatography. Three fractions, D-PG, C-1 PG, and C-2 PG, were separated by CM-Sephadex C-25 column chromatography and FPLC Mono Q HR 5/5 or Mono S HR 5/5 column and their physicochemical characteristics were investigated. All three fractions had optimum pH and temperature of 4.5 and $65^{\circ}C$, and were stable in the range of pH $4.5{\sim}8.0$. These fractions were inhibited by 0.8mM $CaCl_2$ or 0.6M NaCl. In the thermal inactivation of PC isozymes at $65{\sim}80^{\circ}C$, z-values were $8.4{\sim}9.3^{\circ}C$ and D-values at $80^{\circ}C$ were In the range of $120{\sim}126s$. Thermodynamic constants were calculated from thermal inactivation data of the isozymes and were applicable to estimation of thermal process time of retort pouched Kimchi.

• Journal of Korean Society of Water and Wastewater
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• v.31 no.4
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• pp.281-287
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• 2017

Mill scale, an iron waste, was used to separate magnetite particles for the adsorption of phosphate from aqueous solution. Mill scale has a layered structure composed of wustite (FeO), magnetite ($Fe_3O_4$), and hematite ($Fe_2O_3$). Because magnetite shows the highest magnetic property among these iron oxides, it can be easily separated from the crushed mill scale particles. Several techniques were employed to characterize the separated particles. Mill scale-derived magnetite particles exhibited a strong uptake affinity to phosphate in a wide pH range of 3-7, with the maximum adsorptive removal of 100%, at the dosage of 1 g/L, pH 3-5. Langmuir isotherm model well described the equilibrium data, exhibiting maximum adsorption capacities for phosphate up to 4.95 and 8.79 mg/g at 298 and 308 K, respectively. From continuous operation of the packed-bed column reactor operated with different EBCT (empty bed contact time) and adsorbent particle size, the breakthrough of phosphate started after 8-22 days of operation. After regeneration of the column reactor with 0.1N NaOH solution, 95-98% of adsorbed phosphate could be detached from the column reactor.

• Structural Engineering and Mechanics
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• v.68 no.2
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• pp.193-201
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• 2018

This paper describes an experimental study on the seismic performance of steel reinforced concrete (SRC) T-shaped columns. The lateral loads were applied along the web of the column with different loading histories, such as monotonic loading, mixed loading of variable amplitude cyclic loading and monotonic loading, constant amplitude cyclic loading and variable amplitude cyclic loading. The failure modes, load-displacement curves, characteristic loads and displacements, ductility, strength and stiffness degradations and energy dissipation capacity of the column were analyzed. The effects of loading history on the seismic performance were focused on. The test results show that the specimens behaved differently in the aspects of the failure mode subject to different loading history, although all the failure modes can be summarized as flexural failure. The hysteretic loops of specimens are plump, and minimum values of the failure drift angles and ductility coefficients are 1/24 and 4.64, respectively, which reflect good seismic performance of SRC T-shaped column. With the increasing numbers of loading cycles, the column reveals lower bearing capacity and ductility. The strength and stiffness of the column with variable amplitude cyclic loading degrades more rapidly than that with constant amplitude cyclic loading, and the total cumulative dissipated energy of the former is less.

• Journal of Korean Society of Steel Construction
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• v.20 no.5
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• pp.645-653
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• 2008

Welded built-up square tubes are manufactured by flare welding at the center of the column width for cold-formed L-shaped four-piece plates and improved composite effect of concrete and steel by vertical inner anchor. Also, the axial resistance of concrete is increased by the thinness of the steel column, and the composite effect of concrete and steel prevents the steel column from local buckling. In this study, we introduced a manufacturing method of built-up square column steel square concrete-filled tubular column with vertical inner anchor and superior structural performance of the square stub column verified by the structural test for 15 specimens with parameters of shape of tube (built-up square tube, general steel tube), width over thickness of the steel tube (B/t=50, 58, 67) and the strength of concrete (f'c=10MPa, 50MPa).

• Korean journal of applied entomology
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• v.16 no.4 s.33
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• pp.221-227
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• 1977

Polychlorinated Biphenyls (PCBs) interfere with gas chromatographic analysis of multiple organochlorine pesticide residues. In the present work, existing Florisil column chromatographic method has been modified as to improve separation of organochlorine pesticides and their metabolites from PCBs. It was amply demonstrated that separation of $\alpha-BHC,\; \gamma-BHC$ Heptachlor epoxide Dieldrin, p.p-DDD, p.p'-DDT from PCBs such as Aroclor 1254 is complete and recovery of the pesticides is found quantitative. Aldrin and Heptachlor in the Aroclor eluant can be separately analyzed by comparison of the chromatographic pattern of standard Aroclor 1254 with that of Aroclor 1254 a dmixed with the two pesticides. The Florsil column technique can be utilized in the routine evaluation of the organochlorine pesticide residues by gas chromatography.

• Environmental Engineering Research
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• v.19 no.4
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• pp.345-354
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• 2014

In this paper, effects of physical configurations and operating conditions on bubble column performance were analyzed in terms of bubble hydrodynamic and mass transfer parameters. Bubble column with 3 different dimensions and 7 gas diffusers (single / multiple orifice and rigid / flexible orifice) were applied. High speed camera and image analysis program were used for analyzing the bubble hydrodynamic parameters. The local liquid-side mass transfer coefficient ($k_L$) was estimated from the volumetric mass transfer coefficient ($k_La$) and the interfacial area (a), which was deduced from the bubble diameter ($D_B$) and the terminal bubble rising velocity ($U_B$). The result showed that the values of kLa and a increased with the superficial gas velocity (Vg) and the size of bubble column. Influences of gas diffuser physical property (orifice size, thickness and orifice number) can be proven on the generated bubble size and the mass transfer performance in bubble column. Concerning the variation of $k_L$ coefficients with bubble size, 3 zones (Zone A, B and C) can be observed. For Zone A and Zone C, a good agreement between the experimental and the predicted $K_L$ coefficients was obtained (average difference of ${\pm}15%$), whereas the inaccuracy result (of ${\pm}40%$) was found in Zone B. To enhance the high $k_La$ coefficient and absorption efficiency in bubble column, it was unnecessary to generate numerous fine bubbles at high superficial gas velocity since it causes high power consumption with the great decrease of $k_L$ coefficients.

• Structural Engineering and Mechanics
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• v.66 no.4
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• pp.531-542
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• 2018

In this paper an analytical model in a closed form able to reproduce the monotonic flexural response of external RC beam-column joints with smooth rebars is presented. The column is subjected to a constant vertical load and the beam to a monotonically increasing lateral force applied at the tip. The model is based on the flexural behavior of the beam and the column determined adopting a concentrated plasticity hinge model including slippage of the main reinforcing bars of the beam. A simplified bilinear moment-axial force domain is assumed to derive the ultimate moment associated with the design axial force. For the joint, a simple truss model is adopted to predict shear strength and panel distortion. Experimental data recently given in the literature referring to the load-deflection response of external RC joints with smooth rebars are utilized to validate the model, showing good agreement. Finally, the proposed model can be considered a useful instrument for preliminary static verification of existing external RC beam-column joints with smooth rebars for both strength and ductility verification.

• Architectural research
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• v.2 no.1
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• pp.55-60
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• 2000

Recently, there is a great demand for precast reinforced concrete (RC) construction methods on the purpose of simplicity in construction. Nishimatsu Construction Company has developed a construction method with precast reinforced concrete members in medium-rise building. In this construction method, how to joint precast members, especially the anchorage of the main bar of beam, is important problem. In this study, the structural performance of exterior joints with precast members was investigated. The parameters of the test specimens are anchorage type of the main bar of beam (U-shape anchorage or anchorage plate) and the ratio of the column axial force to the column strength. Specimens J-3 and J-4 used U-shape anchorage and the ratio of the column axial force of specimen J-4 was higher. On the other hand, specimens J-5 and J-6 used anchorage plate, and the anchorage lengths are 15d and 18d, respectively. Experimental results are summarized as follows; 1) For the joints with beam flexural failure mode, it was found that the maximum strength of specimen with anchorage plate is equal to or larger than that of specimen with conventional U-shaped anchorage if the anchorage length of more than 15d would be ensured, 2) Each specimen shows stable hysteretic curves and there were no notable effects on the hysteretic characteristics and the maximum strength caused by the anchorage method of beam main bar and the difference of column axial stress level.

• Microbiology and Biotechnology Letters
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• v.28 no.6
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• pp.322-328
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• 2000

To screen agent for the treat-ment of Alzhimers Disease several strains of bacteria producing acetylcholinesterase inhibitor ware isolated from soil. Strain 960903 showed strong acetylcholinesteras inhibitory activity and low butyrylcholinesterse inhibitory activity. The strain 960903 was identified as Pseudomonas sp. Acetylcholinesterase inhibitor ws highly achieved in fermentation medium containing soluble starch 3.0%, glycerol 1.0%, pharmamedia 0.5%, KCI 0.3%, $CaCO_3$ 0.2%, MgS $O_4$..$7H_2$O 0.05%, $KH_2$$PO_4$ 0.05%(pH6.5) at $30^{\circ}C$ for 4 days. Acetylcholinesterase inhibitor was purified by Diaion WA-30($OH^{-}$) column charomatography and cellulose column chromatography. Acetylcholinesterase inhibi-tor showd the maximum wavelength at 205 nm and was soluble in water, acetic acid, ethanol, methanol and dime-thyl sulfoxide. The concentration of 50% inhibition($IC_{50}$) of inhibitor against acetylcholinesterase was 25$\mu\textrm{g}$/ml. The inhibitor was inactivated on heating ar $100^{\circ}C$ fro 15 min and more stable in acidic region than alkaline region.n.

• YAKHAK HOEJI
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• v.32 no.4
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• pp.251-257
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• 1988

A simple and sensitive HPLC method was developed for the simultaneous determination of ten kinds of active ingredients formulated in commercial cough-cold mixtures. A group of Pseudoephedrine HCl, dl-Methylephedrine HCl, Noscapine, Chlorophenylamine maleate, Dextromethorphan HBr and Phenylpropanolamine HCl were determined at 254nm using a Novapak $C_{18}$ column with mobile phase consisting of a mixture of methanol-acetonitrile-1, 4dioxane-tetrahydrofuran-water(12 : 20 : 20 : 5 : 43, pH4.7) containing 0.013M-dioctyl sodium sulfosuccinate. The another group of Acetoaminophen, Caffeine, Guaifenesin and Ethenzamide were also determined at 254nm using a Novapak $C_{18}$ column as the stationary phase, and a mixture of methanol-1% aqueous acetic acid (3 : 7). The results indicate that these methods are accurate and precise with relative standard deviation of not more than 1% (n=5) for the above active ingredients.