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Anti-tumor Effect of Combined Betacarotene with X-irradiation in the Mouse Fibrosarcoma : Cytotoxicity and Tumor Growth Delay (쥐 섬유육종에서 베타카로틴과 방사선조사 병용의 항종양 효과: 세포독성 및 종양성장 지연에 미치는 영향)

  • Kwon Hyoung-Cheol;Yang Moon-Sik
    • Radiation Oncology Journal
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    • v.18 no.2
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    • pp.133-137
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    • 2000
  • Purpose :To investigate whether combined beta-carotene with X-Irradiation has more enhanced radition response than X-irradiation or not, we peformed a experiment about in vitro cytotoxlcity of beta-carotene and/or X-irradiation in the fibrosarcoma cells, tumor growth delay of combined beta-caroten with/or X-irradiation in the mouse fibrosarcoma. Materials and Methods : 2$\%$ emulsion of beta-carotene was serially diluted and used. X-Irradiation was given by 6 MeV linear accelerator. The cytotoxicity of beta-carotene in vitro was evaluated from clonogenic assay. To compare the cytotoxiclty between combined beta-carotene with X-irradiation and X-irradiation group, 2 mg/ml of beta-carotene was contacted to fibrosarcoma (FSall) cells for 1 hour before X-irradiation. For the tumor growth delay, single 20 Gy was given to FSall tumor hearing C3H/N mice whic was classified as beta-crotene with X-irradiation group (n=5) and X-irradiation alone group (n=5). 0.2 ml of 20 mg/kg of beta-carotene were i.p. injected to mice 30 minute before X-irradiation in the beta-crotene with X-irradiation group. The tumor growth delay defined as the time which reach to 1,000 mm$^{3}$ of tumor volume. Results : (1) Cytotoxicity in vitro: 1) survival fraction at beta-carotene concentration of 0.002,0.02,0.2 and 2 mg/ml were 0.69$\pm$0.07, 0.59$\pm$0.08, 0.08$\pm$0.008 and 0.02$\pm$0.006, respectively. 2) each survival fraction at 2, 4, 6 and 8 Gy in the 2 mg/ml of beta-carotene + X-irradiation group were 0.13$\pm$0.05, 0.03$\pm$0.005, 0.01 $\pm$0.002 and 0.009$\pm$0.0008, respectively. But each survival fraction at same irradiation dose in the X-irradiation group were 0.66$\pm$0.05, 0.40$\pm$0.04, 0.11$\pm$0.01 and 0.03$\pm$0.006, respectively(p<0.05). (2) The time which reach to 1,000 mm$^{3}$ of tumor volume of beta-carotene + X-irradiation group and X-irradiation alone group were 18, 19 days, respectively(p>0.05) Conclusion : The contact of beta-caroten to Fsall cells showed mild cytotoxicity which 띤as increased according to concentration. The cytotoxicity of combined beta-carotene with X-irradiation more increased than that of X-irradiation, additionally, And there was significant difference of cytotoxicity between two groups. But there were no significant difference of the growth delay of fibrosarcoma between two groups.

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The Effects of Silicate Nitrogen, Phosphorus and Potassium Fertilizers on the Chemical Components of Rice Plants and on the Incidence of Blast Disease of Rice Caused by Pyricularia oryzae Cavara (규산 및 삼요소 시비수준이 도체내 성분함량과 도열병 발생에 미치는 영향)

  • Paik Soo Bong
    • Korean journal of applied entomology
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    • v.14 no.3 s.24
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    • pp.97-109
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    • 1975
  • In an attempt to develop an effective integrated system of controlling blast disease of rice caused by Pyricularia oryzae Cav., the possibility of minimizing the disease incidence by proper application of fertilizers has been investigated. Thus the effect of silicate, nitrogen, phosphorus and potassium fertilizers on the development of blast disease as well as the correlation between the rice varieties an4 strains of P. oryzae were studied. The experiments were made in 1971 and 1973 by artificial inoculation and under natural development of the blast disease on rice plants. The results obtained are summarized as follows. 1. Application of silicate fertilizer resulted in the increase of silicate as well as total sugar and potassium content but decrease of total nitrogen and phosphorus in tile leaf blades of rice plants. 2. The ratios of total C/total N. $ SiO_2/total$ N, and $K_2O/total$ N in leaf blades of rice plants increased by the application of silicate fertilizers. There was high level of negative correlation between the ratios mentioned above and the incidence of rice blast disease. 3. Application of silicate fertilizer reduced the incidence of rice blast disease. 4. The over dressing of nitrogen fertilizer resulted in the increase of total nitrogen and decrease of silicate and total sugar content in leaf blades, thus disposing the rice plants more susceptible to blast disease. 5. Over dressing of phosphorus fertilizer resulted in the increase of both total nitrogen and Phosphorus, and decrease of silicate content in the leaf blades inducing the rice plants to become more susceptible to blast disease. 6. Increased dressing of potash resulted in the increase of silicate content and $K_2O/total$ N ratio but decrease of total nitrogen content in leaf blades. When potassium content is low in the leaf blades of rice plants, the additional dressing of potash to rice plant contributed to the increase of resistance to blast disease. However, there was no significant correlation between additional potassium application and the resistance to blast disease when the potassium content is already high in the leaf blades. 7. When four rice varieties were artificially inoculated with three strains of P. oryzae, the incidence of blast disease was most severe on Pungok, least severe on Jinheung and moderate on Pungkwang and Paltal varieties. 8. Disease incidence was most severe on the second leaf from top and less sever on top and there leaf regardless of the fertilizer application when 5-6 leaf stage rice seedlings of four rice varieties were artificially inoculated with three strains of P. oryzae. 9. The pathogenicity of three strains of P. oryzae was in the order of $P_1,\;P_2,\;and\;P_3$ in their virulence when inoculated to Jinheung, Paltal, Pungkwang varieties but not with Pungok. The interaction between strains of P. oryzae and rice varieties was significant.

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Synthesis of Titanium Dioxides Using Low Temperature Combustion Method and Photocatalytic Decomposition of Methylene Blue (저온연소법에 의한 이산화티탄의 합성 및 메틸렌블루의 광촉매 분해반응)

  • Baek, Seung Hee;Jung, Won Young;Lee, Gun Dae;Park, Seong Soo;Hong, Seong-Soo
    • Applied Chemistry for Engineering
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    • v.20 no.3
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    • pp.329-334
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    • 2009
  • Yttrium ions doped $TiO_2$ particles have been prepared using a low temperature combustion method. The physical properties were investigated, together with the activity of $TiO_2$ particles as a photocatalyst for the decomposition of methylene blue. From XRD results, the major phase of all the $TiO_2$ particles prepared under basic condition was an anatase structure but a rutile peak was observed when they are prepared under acidic condition. The crystallite size of $TiO_2$ particles was decreased as the molar ratio of CA/TTIP increased. The photocatalytic activity increased with an increase of CA/TTIP molar ratio and pH in the solution. In addition, the doping of 1.0 mole% yttrium ion on the $TiO_2$ enhanced the photocatalytic activity and showed the higher activity than commercial P-25 catalyst.

Statistical Characteristics of Solar Wind Dynamic Pressure Enhancements During Geomagnetic Storms

  • Choi, C.R.;Kim, K.C.;Lee, D.Y.;Kim, J.H.;Lee, E.
    • Journal of Astronomy and Space Sciences
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    • v.25 no.2
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    • pp.113-128
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    • 2008
  • Solar wind dynamic pressure enhancements are known to cause various types of disturbances to the magnetosphere. In particular, dynamic pressure enhancements may affect the evolution of magnetic storms when they occur during storm times. In this paper, we have investigated the statistical significance and features of dynamic pressure enhancements during magnetic storm times. For the investigation, we have used a total of 91 geomagnetic storms for 2001-2003, for which the Dst minimum $(Dst_{min})$ is below -50 nT. Also, we have imposed a set of selection criteria for a pressure enhancement to be considered an event: The main selection criterion is that the pressure increases by ${\geq}50%\;or\;{\geq}3nPa$ within 30 min and remains to be elevated for 10 min or longer. For our statistical analysis, we define the storm time to be the interval from the main Dst decrease, through $Dst_{min}$, to the point where the Dst index recovers by 50%. Our main results are summarized as follows. $(i){\sim}$ 81% of the studied storms indicate at least one event of pressure enhancements. When averaged over all the 91 storms, the occurrence rate is ${\sim}$ 4.5 pressure enhancement events per storm and ${\sim}$ 0.15 pressure enhancement events per hour. (ii) The occurrence rate of the pressure enhancements is about three times higher for CME-driven storm times than for CIR-driven storm times. (iii) Only 21.1% of the pressure enhancements show a clear association with an interplanetary shock. (iv) A large number of the pressure enhancement events are accompanied with a simultaneous change of IMF $B_y$ and/or $B_z$: For example, 73.5% of the pressure enhancement events are associated with an IMF change of either $|{\Delta}B_z|>2nT\;or\;|{\Delta}B_y|>2nT$. This last finding suggests that one should consider possible interplay effects between the simultaneous pressure and IMF changes in many situations.

Quantitatation of Benzoic and Sorbic Acids in Some Processed Foods of Korea (일부 한국 가공식품의 벤조산과 소르빈산의 함량 조사)

  • Mitra, Sanika;Han, Jang-Il;Lee, Ki-Teak;Kim, Seong-Ai
    • Food Science and Preservation
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    • v.16 no.6
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    • pp.789-796
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    • 2009
  • Benzoic acid (BA) and sorbic acid (SA) are the preservatives most commonly used in food. Although BA and SA are generally safe, some previous studies have shown that consumption of excessive amounts of these food additives can be a health hazard. The aim of this study was to determine the amounts of BA and SA in processed foods in Korea. Different brands of fruit juice, yogurt, cheese, dried fruits, jam, and margarine were purchased at a local market in Daejeon, Korea. Samples were analyzed by high-performance liquid chromatography (HPLC) using a UV detector. Chromatographic separation was achieved with a C18 column. Methanol acetate buffer (pH 4.4) at a 35:65 v/v ratio was used as the initial mobile phase to elute BA and SA. The detector wavelength was set at 254 nm. The average test results observed for BA concentrations in fruit juice, yogurt, cheese, dried fruits, jam, and margarine were $40.26{\pm}0.02$, $2.07{\pm}0.06$, $0.02{\pm}0.09$, $0.36{\pm}08$, $265.30{\pm}0.02$, and $27.34{\pm}0.08\;mg/kg$, respectively. Average concentrations of SA in these samples were $0.92{\pm}0.06$, $1.06{\pm}0.07$, $7.30{\pm}0.01$, $14.14{\pm}0.08$, $25.65{\pm}0.06$, and $4.81{\pm}0.07\;mg/kg$, respectively. Thus, the average levels of BA and SA in the studied food items were lower than the KFDA-permitted limits. Moreover, the estimated daily intake of both BA and SA by a typical consumer were below the maximum recommended daily values.

Determination of the Optimum Dose Range for a Mutation Induction of Turfgrasses by a Gamma-Ray (잔디류 돌연변이 유기를 위한 적정 방사선 선량범위의 결정)

  • Lee, Hye-Jung;Lee, Geung-Joo;Kim, Dong-Sub;Kim, Jin-Baek;Ku, Ja-Hyeong;Kang, Si-Yong
    • Asian Journal of Turfgrass Science
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    • v.22 no.1
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    • pp.25-34
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    • 2008
  • This study was conducted to determine the optimum dose ranges for a mutation breeding based on the observations of a seed germination and an early growth in turfgrasses. Three warm season (Zoysiagrass, Bermudagrass, and Seashore paspalum) and four cool season turfgrasses (Kentucky bluegrass, Tall fescue, Perennial ryegrass, and Creeping bentgrass) were used in this study. We investigated the percentage of a seed germination and a seedling growth after irradiating the turfgrass seeds with various doses of gamma-ray (50, 100, 150, 200, 250, 300, 400, and 500 Gy). After 24 h with a gamma irradiation, the seeds were sown on the wet filter paper in a petri dish and maintained for 3 weeks at 30$^{\circ}C$ for the warm season turfgrasses and at 25$^{\circ}C$ for the cool season turfgrasses. Data on a seed germination and a seedling growth with three replications were collected. The percentage of seed germination was decreased with an increase of the gamma-ray dose. Shoot and root growth, and the fresh weight were decreased significantly as the radiation dose was increased. A radiation dose indicating a 50% growth inhibition ($LD_{50}$) with a gamma irradiation was varied among those turfgrass species used, with the highest at about 500 Gy for bermudagrass and the lowest at 100Gy for tall fescue. The optimum dose for a gamma irradiation for a selection of turfgrass mutants was considered to be about 300, 150, 500, 150, 200, 100 and 200 Gy for zoysiagrass, seashore paspalum, bermudagrass, Kentucky bluegrass, perennial ryegrass, tall fescue, and creeping bentgrass, respectively.

Numerical analysis of water flow characteristics after inrushing from the tunnel floor in process of karst tunnel excavation

  • Li, S.C.;Wu, J.;Xu, Z.H.;Li, L.P.;Huang, X.;Xue, Y.G.;Wang, Z.C.
    • Geomechanics and Engineering
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    • v.10 no.4
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    • pp.471-526
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    • 2016
  • In order to investigate water flow characteristics after inrushing in process of karst tunnel excavation, numerical simulations for five case studies of water inrush from the tunnel floor are carried out by using the FLUENT software on the background of Qiyueshan high risk karst tunnel. Firstly, the velocity-distance curves and pressure-distance curves are drawn by selecting a series of probing lines in a plane. Then, the variation characteristics of velocity and pressure are analyzed and the respective optimized escape routes are made. Finally, water flow characteristics after inrushing from the tunnel floor are discussed and summarized by comparing case studies under the conditions of different water-inrush positions and excavation situations. The results show that: (1) Tunnel constructors should first move to the tunnel side wall and then escape quickly when water inrush happens. (2) Tunnel constructors must not stay at the intersection area of the cross passage and tunnels when escaping. (3) When water inrush from floor happens in the left tunnel, if tunnel constructors meet the cross passage during escaping, they should pass through it rapidly, turn to the right tunnel and run to the entrance. (4) When water inrush from floor happens in the left tunnel, if there is not enough time to escape, tunnel constructors can run to the trolley and other equipment in the vicinity of the right tunnel working face. In addition, some rescuing equipment can be set up at the high location of the cross passage. (5) When water inrush from floor happens in the cross passage, tunnel constructors should move to the tunnel side wall quickly, turn to the tunnel without water inrush and run to the entrance. (6) When water inrush from floor happens in the cross passage, if there is not enough time to escape, tunnel constructors can run to the trolley and other equipment near by the left or the right tunnel working face. The results are of important practical significance and engineering value to ensure the safety of tunnel construction.

Solidification/Stabilization of Arsenic Contaminated Soil Using Cement-Based Synthesized Materials (시멘트계 합성물질을 이용한 비소 오염 토양의 고형화/안정화)

  • Kim, Ran;YHong, Seong Hyeok;Jung, Bahng Mi;Chae, Hee Hun;Park, Joo Yang
    • Journal of the Korean GEO-environmental Society
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    • v.13 no.2
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    • pp.59-65
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    • 2012
  • Solidification/Stabilization(S/S) is one of the remediation technologies that have been applied for treating inorganic hazardous wastes. This study investigated the reduction of arsenic concentration of arsenic-contaminated soil using by S/S. The binder plays a role in controlling the mobility and solubility of the contaminants in S/S process, so it is important to determine the optimum binder content. Therefore, this study evaluated the effectiveness of S/S using four different binders(cement, zero valent iron, and monosulfate and ettringite(cement-based synthesized materials) at the binder content ranged between 5%(wt.) and 20%(wt.). The leachability of arsenic in 1 N HCl was different depending on the types of binders: cement(71.41%) > monosulfate(47.45%) > ettringite(46.36%) > ZVI(33.08%) at the binder content of 20%. Additionally, three kinds of a mixture binder were prepared using cement and additives(monosulfate, ettringite, calcium sulfoaluminate(CSA)) and tested for arsenic reduction. The highest arsenic removal capacity was found at the mass ratio of cement to the additive, 4:1 in all experiments using a mixture binder, regardless of the additives types. A mixture binder(cement and additives) resulted in higher arsenic removal relative to the arsenic removal when cement was used alone.

Effect of Nitrous Oxide (N2O) Treatment on Quality of Peach (Prunus persica) Postharvest (복숭아(Prunus persica) 수확 후 아산화질소(N2O) 처리가 품질에 미치는 영향)

  • Nah, Hyun-Seok;Bae, Ro-Na;Lee, Seung-Koo
    • Horticultural Science & Technology
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    • v.30 no.1
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    • pp.42-49
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    • 2012
  • This experiment was conducted to find out the effects of nitrous oxide ($N_2O$) on the postharvest quality of 'Janghowon hwangdo' peach fruits. Fruits were harvested at commercial maturity for marketing in late September, and treated with 70% $N_2O$ + 20% $O_2$ + 10% air, 80% $N_2O$ + 20% $O_2$, and 90% $N_2O$ + 10% $O_2$ for 48 h, and then stored at $15^{\circ}C$. No significant treatments for soluble sugar and titratable acidity contents were detected. However, good appearance and taste in peach fruit were maintained better in 80% $N_2O$ treatment than in air treatment. The treatment with 90% $N_2O$ had negative effects on weight loss and taste because of rotting by anaerobic fermentation. 80% $N_2O$ treated fruit had significantly higher fungus (Botrytis cinerea) growth inhibition of saprogenic approximately than air treatment until 12 days of storage. The browning and rotting at surface of peach were also retarded when peaches were treated with 80% $N_2O$ before they were artificially wounded. The activity of polyphenol oxidase (PPO) was inhibited about 80% in peach of 80% $N_2O$ treatment compared with in air treatment. The result showed that 80% $N_2O$ treatment was able to extend the shelf life of peach fruits through maintaining taste and inhibition of softening and browning by rotting and wounding during storage.

Characteristics of ZnO Nanorod/ZnO/Si(100) Grown by Hydrothermal Method (수열법으로 성장한 ZnO Nanorod/ZnO/Si(100)의 특성)

  • Jeong, Min-Ho;Jin, Yong-Sik;Choi, Sung-Min;Han, Duk-Dong;Choi, Dae-Kue
    • Korean Journal of Materials Research
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    • v.22 no.4
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    • pp.180-184
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    • 2012
  • Nanostructures of ZnO, such as nanowires, nanorods, nanorings, and nanobelts have been actively studied and applied in electronic or optical devices owing to the increased surface to volume ratio and quantum confinement that they provide. ZnO seed layer (about 40 nm thick) was deposited on Si(100) substrate by RF magnetron sputtering with power of 60 W for 5 min. ZnO nanorods were grown on ZnO seed layer/Si(100) substrate at $95^{\circ}C$ for 5 hr by hydrothermal method with concentrations of $Zn(NO_3)_2{\cdot}6H_2O$ [ZNH] and $(CH_2)_6N_4$ [HMT] precursors ranging from 0.02M to 0.1M. We observed the microstructure, crystal structure, and photoluminescence of the nanorods. The ZnO nanorods grew with hexahedron shape to the c-axis at (002), and increased their diameter and length with the increase of precursor concentration. In 0.06 M and 0.08 M precursors, the mean aspect ratio values of ZnO nanorods were 6.8 and 6.5; also, ZnO nanorods had good crystal quality. Near band edge emission (NBE) and a deep level emission (DLE) were observed in all ZnO nanorod samples. The highest peak of NBE and the lower DLE appeared in 0.06 M precursor; however, the highest peak of DLE and the lower peak of NBE appeared in the 0.02 M precursor. It is possible to explain these phenomena as results of the better crystal quality and homogeneous shape of the nanorods in the precursor solution of 0.06 M, and as resulting from the bed crystal quality and the formation of Zn vacancies in the nanorods due to the lack of $Zn^{++}$ in the 0.02 M precursor.