• Title/Summary/Keyword: C3G

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Molecular Discrimination of Dinoflagellates Cochlodinium Polykrikoides Margalef, Gyrodinium Impudicum Fraga et Bravo and Gymnodinium Catenatum Graham using RAPD-PCR Method (RAPD-PCR 방법을 이용한 Cochlodinium polykrikoides Gyrodinium impudicum, Gymnodinium catenatum의 분자생물학적 진단)

  • Cho, Eun-Seob
    • Journal of Life Science
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    • v.13 no.5
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    • pp.651-657
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    • 2003
  • Randomly amplified polymorphic DNA (RAPD) analysis was used to study genetic relationships among C. polykrikoides, G. impudicum and G. catenatum, which possess similar morphological features. Four of 12 primers were selected and 59 amplification products ranged from 0.2 kb to 3.0 kb. The number of polymorphic products in C. polykrikoides, G. impudicum and G. catenatum was 16 (27.1%), 8 (13.5%), and 16 (27.1%), respectively, while 17 were monomorphic. Number of species-specific bounds was 26 (44.0%), 34 (57.6%), 26 (44.0%) in C. polykrikoides, G. impudicum and G. catenatum, respectively. The genetic similarity between C. polykrikoides and G. impudicum/G. catenatum was 0.83, whereas G. impudicum and G. catenatum was 0.78. Our results suggest that C. polykrikoides, G. impudicum and G. catenatum are extremely different on the basis of RAPD analysis, despite similarity based on their morphology. The RAPD technique appears to be efficient in detecting genetic variation in these dinoflagellates.

The Four Color Algorithm (4-색 알고리즘)

  • Lee, Sang-Un
    • Journal of the Korea Society of Computer and Information
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    • v.18 no.5
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    • pp.113-120
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    • 2013
  • This paper proposes an algorithm that proves an NP-complete 4-color theorem by employing a linear time complexity where $O(n)$. The proposed algorithm accurately halves the vertex set V of the graph $G=(V_1,E_1)$ into the Maximum Independent Set (MIS) $\bar{C_1}$ and the Minimum Vertex Cover Set $C_1$. It then assigns the first color to $\bar{C_1}$ and the second to $\bar{C_2}$, which, along with $C_2$, is halved from the connected graph $G=(V_2,E_2)$, a reduced set of the remaining vertices. Subsequently, the third color is assigned to $\bar{C_3}$, which, along with $C_3$, is halved from the connected graph $G=(V_3,E_3)$, a further reduced set of the remaining vertices. Lastly, denoting $C_3$ as $\bar{C_4}$, the algorithm assigns the forth color to $\bar{C_4}$. The algorithm has successfully obtained the chromatic number ${\chi}(G)=4$ with 100% probability, when applied to two actual map and two planar graphs. The proposed "four color algorithm", therefore, could be employed as a general algorithm to determine four-color for planar graphs.

Misgurnus Anguillicaudatus Growing in Winter Season by Solar Thermal Heating (태양에너지를 이용한 겨울철 미꾸라지 성장에 관한 연구)

  • Jung, Hyun-Chai;Sun, Kyung-Ho;Jo, Jae-Sun;Nam, Sang-Yul
    • Solar Energy
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    • v.11 no.3
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    • pp.31-36
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    • 1991
  • We have maintained the optimum water temperature for misgumus in winter season by solar thermal heating energy. The optimum temperatures for the misgumus anguillicaudatus were experimentally estimated, i.e. for the total length $4{\sim}5cm$ and body weight $1{\sim}2g$, the optimum temperature was $18{\sim}20^{\circ}C$. For the total length $5{\sim}7cm$, body weight $2{\sim}3g$ was $20{\sim}22^{\circ}C$ and for the total length $7{\sim}9cm$, body weight $4{\sim}6g$ was $22{\sim}24^{\circ}C$. The smaller misgumas($1{\sim}3g$) grows relatively slow but the bigger one($4{\sim}8g$) grows relatively fast and total average body weight increment was about $30{\sim}50g$ per month.

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Clinical Features of Severely Constipated Children: Comparison of Infrequent Bowel Movement and Fecal Soiling Groups

  • Lee, Gyung;Son, Jae Sung;Bae, Sun Hwan
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • v.23 no.1
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    • pp.26-34
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    • 2020
  • Purpose: To compare the clinical features, diagnostic findings, and medications of children with infrequent bowel movements or fecal soiling. Methods: This study enrolled 333 children (189 male; age range, 1 month to 18 years) diagnosed with functional constipation by Rome III or IV criteria. We classified them into 3 groups (infrequent bowel movement without fecal soiling [G3-a], infrequent bowel movement with fecal soiling [G3-b], and fecal soiling only [G3-c]) and into 2 subgroups of fecal soiling (G2-b) or not (G2-a). Retrospective data on clinical characteristics, colon transit time (CTT) test results, and medications were collected. The Wilcoxon rank-sum test, Kruskal-Wallis test, Chi-square test, and Fisher's exact test were used for the statistical analysis. Results: The median age (months) and interquartile range (IQR) was 33 (45) in G3-a, 54 (40) in G3-b, and 73 (48) in G3-c (p<0.0001). G3-c had the latest onset (median, 18; IQR, 18; p=0.0219) and longest symptom duration (24 [24], p=0.0148). PEG 4000 was used in 60.6% (G3-a), 96.8% (G3-b), and 83.2% (G3-c) of patients (p<0.0001). The median age (months) and IQR were 33.0 (45.0) in G2-a and 63.5 (52.5) in G2-b (p<0.0001). G2-b had later onset (median, 12; IQR, 19.5; p=0.0062) and longer symptom duration than G2-a (24 [12], p=0.0070). PEG 4000 was used in 60.6% (G2-a) and 88.3% (G2-b) of children (p<0.0001). No statistically significant intergroup differences were seen in maintenance laxative dose, CTT, or CTT type. Conclusion: Infrequent bowel movement and fecal soiling represent the advanced stage of chronic functional constipation.

Distribution of Indicator Organisms in Commercial Fish and Shellfish and Influence of Storage Temperature and Period (시판 어패류에서의 오염지표세균의 분포와 저장 온도 및 저장 기간이 오염지표세균에 미치는 영향)

  • 박석기;이용욱;김정현;이강문
    • Journal of Food Hygiene and Safety
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    • v.11 no.1
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    • pp.57-70
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    • 1996
  • This paper intends to investigate commercial fish and shellfish 25 species (fish 8 species, shellfish 7 species, crustacean 3 species, moluse 4 species and echinodermata) for the distribution of sanitary indicator organisms (total viable counts, coliforms, staphylococci, vibrios, and enterococci) and distributional change of indicator organisms accordion to storage temperature and period. The logarithmic mean of total viable counts for total commercial fish and shellfish 25 species was 5.41$\pm$.026 CFU/g, and in accordance with fish and shellfishes, crustacean 6.76$\pm$0.67 CFU/g, shellfish 5.67$\pm$0.56 CFU/g, echinodermata 5.47$\pm$1.50 CFU/g, fish 5.02$\pm$0.38 CFU/g, and mollusc 5.03$\pm$0.65 CFU/g. The logarithmic mean of enterococci was 2.36$\pm$0.37 CFU/g, and in accordane with fish and shellfish, crustacean 3.44$\pm$2.12 CFU/g, shellfish 3.87$\pm$0.45 CFU/g, echinodermata 3.38$\pm$0.0 CFU/g, fish 2.16$\pm$0.41 CFU/g and mollusc 0.01$\pm$0.0 CFU/g. The logarithmic mean of vibrios was 1.60$\pm$1.59 CFU/g, and in accordance with fish and shellfish, crustacean 4.23$\pm$1.11 CFU/g, shellfish 3.58$\pm$0.90 CFU/g, echinodermata 1.64$\pm$0.34 CFU/g, fish 1.79$\pm$0.67 CFU/g and mollusc 1.07$\pm$0.61 CFU/g. The logarithmic mean of staphylococci was 1.60$\pm$1.59 CFU/g, and in accordance with fish and shellfish, shellfish 0.01$\pm$0.00 CFU/g, echinodermata 3.51$\pm$0.60 CFU/g, fish 1.68$\pm$0.64 CFU/g, crustacean 0.34$\pm$0.33 and mollusc 3.90$\pm$0.11 CFU/g. The logarithmic mean of coliformas was 2.52$\pm$0.32 CFU/g, and in accordance with fish and shellfish, echinodermata 3.58$\pm$1.89 CFU/g was highest, shellfish 3.25$\pm$0.30 CFU/g, crustacean 3.23$\pm$0.49 CFU/g, fish 2.18$\pm$0.63 CFU/g, peeled shellfish 1.80$\pm$0.51 CFU/g and mollusc 1.55$\pm$0.95 CFU/g. As the results of research of the change of the contaminate indicator microflora in working with storage period at 1$0^{\circ}C$, 2$0^{\circ}C$ and 3$0^{\circ}C$, total viable counts was increased without storage temperature and enterococci were decreased slowly at 1$0^{\circ}C$, but increased at 20 and 3$0^{\circ}C$. Vibrios were decreased slowly at 1$0^{\circ}C$, decreased at 2$0^{\circ}C$ and 3$0^{\circ}C$in 2 days after increased rapidly. Staphylococci were increased promptly without storage temperature in 2 days, then the total viable counts were maintained. Coliforms were increased at 1$0^{\circ}C$ by 7 days, then decreased or maintained after 14 days, changed at 2$0^{\circ}C$ in accordance with fish species in 2 days, then returned to the initial total viable count, and decreased rapidly at 3$0^{\circ}C$ on 2 days. By the way, there were no difference among the species.

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Connected geodesic number of a fuzzy graph

  • Rehmani, Sameeha;Sunitha, M.S.
    • Annals of Fuzzy Mathematics and Informatics
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    • v.16 no.3
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    • pp.301-316
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    • 2018
  • In this paper, the concept of connected geodesic number, $gn_c(G)$, of a fuzzy graph G is introduced and its limiting bounds are identified. It is proved that all extreme nodes of G and all cut-nodes of the underlying crisp graph $G^*$ belong to every connected geodesic cover of G. The connected geodesic number of complete fuzzy graphs, fuzzy cycles, fuzzy trees and of complete bipartite fuzzy graphs are obtained. It is proved that for any pair k, n of integers with $3{\leq}k{\leq}n$, there exists a connected fuzzy graph G : (V, ${\sigma}$, ${\mu}$) on n nodes such that $gn_c(G)=k$. Also, for any positive integers $2{\leq}a<b{\leq}c$, it is proved that there exists a connected fuzzy graph G : (V, ${\sigma}$, ${\mu}$) such that the geodesic number gn(G) = a and the connected geodesic number $gn_c(G)=b$.

Study on the Estimation of Selection Index in Broiler Breeder I. Estimation of Genetic Parameters in Broiler (육용종계의 선발지수 추정에 관한 연구 I. 육용종계 부계통과 모계통의 유전적 모교추정)

  • 김기경;손시환;오봉국
    • Korean Journal of Poultry Science
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    • v.11 no.2
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    • pp.86-92
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    • 1984
  • Present study was carried out to estimate phenotypic and genetic parameters influencing body weight (BW) at 4 weeks of age egg breadth (EB), egg length(EL), egg shape index (SI) and egg weight (EW) at 32 weeks of age and egg numbers (EN) up to 38 weeks of age in broiler male and female lines. The data were collected from closed White Plymouth Rock (female line; G) and Cornish (male line; C) flocks involving 1193 pullets from 211 dams and 48 sires in 1982. The results obtained are summarized as follow: 1. General performance for various trails of lines C and G. The means and standard deviations of BW, EB, EL, SI, EW and EN were 668.34${\pm}$47.18, 4.23${\pm}$0,11, 5.49 ${\pm}$0.19, 77.06${\pm}$2.98, 55.73${\pm}$3.54 and 59.72${\pm}$13.39 in line C, respectively and 487.89${\pm}$ 41.43, 4.22${\pm}$0.11, 5.51${\pm}$0.19, 76.72${\pm}$3.20, 55.43${\pm}$3.26 and 76.93${\pm}$12.17 in line G, respectively. 2. Heritability Heritabilities were estimated from sire, dam and combined components. Estimates for BW, EB, EL, SI, EW and EN from combined components were 0.30, 0.29, 0,40, 0.22, 0.45 and 0.60 in line C, respectively and 0.33, 0.23, 0.28, 0.13, 0.49 and. 0.33 in line G, respectively. 3. Correlation Genetic and phenotypic correlations showed similar trend in line C and G. Genetic correlations, estimated EW with EB and EL, were high and positive (line C; 0.99, 0.75, respectively and line G; 0.94, 0.82, respectively), also correlation of EB with EL was 0.58 (both lines; 0.58). High and negative genetic correlations were shown between SI and EL in line C and G (-0.70, -0.65, respectively). Genetic correlations between SI and EW were relatively low and negative in line C and G (-0.11, -0.19, respectively) and between SI and EN were relatively low and positive in line C and G (0.25, 0.17, respectively). Between other traits, low genetic correlations were shown in both lines, High and positive correlation was estimated between hatchability and egg shape index and polynomial regression of egg shape index on hatchability was estimated; Y=-216.77+7.6216X-0.0146939X$^2$.

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Influence of Filling Density in the Positive Active-material on the Cycle-life Performance of the Tubular Type Gelled Valve Regulated Lead Acid Batteries (튜브식 겔형 납축전지에 있어서 활물질 밀도에 따른 싸이클 수명 특성)

  • Yoon, Youn-Saup;Kim, Byung-Kwan;Lee, Soo;Kim, Kyu-Tea
    • Applied Chemistry for Engineering
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    • v.10 no.3
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    • pp.415-418
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    • 1999
  • The characteristics of VRLA (valve regulated Iead-acid) battery with the tubular positive plate and gel type electrolyte were examined as a function of active material filling density. The filling density of positive plate was 3.2 g/mL, 3.4 g/mL, and 3.6 g/mL, respectively. These VRLA batteries were cycled with 100% DOD (depth of discharge) at the $C_5/5$ rate, followed by IU-type recharge with $I_{max}=0.2C_{10}/10$ and a final voltage V=2.40 V/cell. The test was performed in a thermostatic room at $25{\pm}1^{\circ}C$. The result indicated that the initial capacity was independent of active material filling density, i.e., the highest initial capacity was 3.4 g/mL of filling density and the lowest was 3.6 g/mL. On aspect of the cycle-life performance of the VRLA battery, the filling density of 3.6 g/mL was similar to that of 3.4 g/mL in the positive plate, and both were higher than that of 3.2 g/mL. Water-loss and degradation of the VRLA battery were decreased according to an increase of the filling density in the positive plate. The optimum filling density of the active material was 3.4~3.6 g/mL.

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Major gene identification for SREBPs and FABP4 gene which are associated with fatty acid composition of Korean cattle (한우의 지방산 조성에 영향을 미치는 SREBPs와 FABP4의 유전자 조합 규명)

  • Lee, Jae-Young;Jang, Ji-Eun;Oh, Dong-Yep
    • Journal of the Korean Data and Information Science Society
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    • v.26 no.3
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    • pp.677-685
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    • 2015
  • Disease of human and economic traits of livestocks are affected a lot by gene combination effect rather than a single gene effect. In this study, we used SNPHarvester method that supplement existing method in order to investigate the interaction of these genes. The used genes are SREBPs (g.3270+10274 C>T, g.13544 T>C) and FABP4 (g.2634+1018 A>T, g.2988 A>G, g.3690 G>A, g.3710 G>C, g.3977-325 T>C, g.4221 A>G) that are closely related to the fatty acid composition affecting the meatiness of Korean cattle. The economic traits which are used are oleic acid (C18:1), monounsaturated fatty acid (MUFA), marbling score (MS). First, we have utilized the SNPHarvester method in order to find excellent gene combination, and then used the multifactor dimensionality reduction method in order to identify excellent genotype in gene combination.

Comparison of Glomerular Filtration Rate Using Gates Method and Serum Creatinine (Gates 방법과 혈청 크레아티닌을 이용한 사구체 여과율의 비교)

  • Yun, Jong-Jun;Lee, Hyo-Yeong;Lee, Hwa-Jin;Lee, Mu-Seok;Song, Hyeon-Seok;Park, Se-Yun;Jeong, Ji-Uk
    • The Korean Journal of Nuclear Medicine Technology
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    • v.13 no.3
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    • pp.61-66
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    • 2009
  • Purpose: Glomerular filtration rate (GFR) is considered as the best overall index for the level of renal function, diagnosis of doubtful kidney disease, progress observation from chronic kidney disease and is measured with the various methods. In this study, We measured standard GFR by Gates method and attempted to compare the result with serum creatinin-based, Cockcroft-Gault(C-G) formula and Modification of Diet in Renal Disease (MDRD) formula. Materials and methods: 217 patients (127 men, 90 women, mean age $51.3{\pm}16.9$) with various renal function were examined. we compared the GFR using $^{99m}Tc$-DTPA (Gates), C-G formula and MDRD formula. Results: Significant correlations were noted between 2 different GFR estimates (from C-G formula: r=0.864, p<0.0001, MDRD formula: r=0.831, p<0.0001) and $^{99m}Tc$-DTPA (Gates) GFR. Average of serum creatinine (Scr) was measured with $3.0{\pm}3.1\;mg/dL$, In patients with normal renal function (Scr<1.5 mg/dL), $^{99m}Tc$-DTPA (Gates) GFR was statistically significant to C-G formula (p<0.0001) and MDRD formula (p<0.0001). In patients with mild to moderate renal insufficiency (1.5$^{99m}Tc$-DTPA (Gates) GFR was not statistically significant to C-G formula (p=0.181) and MDRD formula (p=0.127). In patients with severe renal insufficiency (Scr>4.0mg/dL), $^{99m}Tc$-DTPA (Gates) GFR was statistically significant to C-G formula and MDRD formula (p<0.0001). Conclusions: Glomerular filtration rate using Gates method was closly correlated to C-G formula and MDRD formula. In patients with normal renal function, $^{99m}Tc$-DTPA (Gates) GFR was significantly lower than C-G formula and MDRD formula. In patients with mild to moderate renal insufficiency, $^{99m}Tc$-DTPA (Gates) GFR was simmilar with C-G formula and MDRD formula. In patients with severe renal insufficiency, $^{99m}Tc$-DTPA (Gates) GFR was significantly higher than C-G formula and MDRD formula. None of the three different methods was clearly superior to the others.

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