• Title/Summary/Keyword: C33F10_2

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Functional Analysis of the Putative BUB2 Homologues of C. elegans in the Spindle Position Checkpoint

  • Lee, Kyung-Hee;Song, Ki-Won
    • Animal cells and systems
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    • v.9 no.2
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    • pp.87-94
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    • 2005
  • Spindle position checkpoint monitors the orientation of mitotic spindle for proper segregation of replicated chromosomes into mother cell and the daughter, and prohibits mitotic exit when mitotic spindle is misaligned. BUB2 forms one of the key upstream element of spindle position checkpoint in budding yeast, but its functional homologues have not been identified in higher eukaryotes. Here, we analyzed the functions of two putative BUB2 homologues of C. elegans in the spindle orientation checkpoint. From the C. elegans genome database, we found that two open reading frames (ORFs), F35H12_2 and C33F10_2, showed high sequence homology with BUB2. We obtained the expressed sequence tag (EST) clones for F35H12_2 (yk221d4) and C33F10_2 (yk14e10) and verified the full cDNA for each ORF by sequencing and 5' RACE with SL1 primer. The functional complementation assays of yk221d4 and yk14e10 in ${\Delta}bub2$ of S. cerevisiae revealed that these putative BUB2 homologues of C. elegans could not replace the function of BUB2 in spindle position checkpoint and mitotic exit. Our attempt to document the component of spindle position checkpoint in metazoans using sequence homology was not successful. This suggests that structural information about its components might be required to identify functional homologues of the spindle position checkpoint in higher eukaryotes.

MEASUREMENT OF THE DENTAL ARCH DIMENSION IN KOREAN YOUNG ADULTS. (한국인청년남자의 치궁에 관한 계측)

  • Kim, Yeong-Hae
    • The Journal of the Korean dental association
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    • v.13 no.1
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    • pp.33-36
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    • 1975
  • To determine the dental arch length and width in Korean yaung adults, various points (shown on Fig 1) were measured by means of Boley gauge on the 124 plaster models which obtained from 2 young men. The measurements value were as follows: A : 36.10㎜ a : 25.53㎜ B : 33.35㎜ b : 28.20㎜ C : 39.80㎜ c : 33.07㎜ D : 43.36㎜ d : 36.36㎜ E : 44.80㎜ e : 39.12㎜ F : 49.11㎜ f : 42.48㎜ G : 59.98㎜ g : 44.92㎜ H : 62.22㎜ h : 57.85㎜ I : 52.66㎜ i : 49.91㎜

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Properties of metal-ferroelectric thin film-silicon(MFS) structure using BaMgF$_{4}$ (BaMgF$_{4}$ 를 이용한 금속-강유전체박막-실리콘(MFS) 구조의 특성)

  • 김광호;김제덕;유병곤
    • Journal of the Korean Institute of Telematics and Electronics A
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    • v.33A no.5
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    • pp.102-107
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    • 1996
  • Use of a rapid thermal annealing (RTA) technique is shown to improve the properties of metal-ferroelectric BaMgF$_{4}$-silicon structures. The fluoride film was deposited in an ultra-high vacuum system at asubstrate temperature of 300$^{\circ}C$. A post-deposition annelaing was conducted for 10 seconds at 600.deg. C in a vacuum of 0.1 Torr, using a home-made RTA apparatus. The results showed that the resistivity of the ferroelectric BaMgF$_{4}$ film from a typical value of 1-2${\times}10^{11}{\Omega}{\cdot}cm$ before the annealing to about 5${\times}10^{13}{\Omega}{\cdot}cm$ and reduce the interface state density of the BaMgF$_{4}$/Si interface to about 8${\times}10^{10}cm^{2}{\cdot}$eV. Ferroelectric hysteresis measurements using a sawyer-tower circuit yielded remanent polarization and coercive field values of about 0.5$\mu$C/cm$^{2}$ and 80 kV/cm, respectively. the typical remanent polarization of the BaMgF$_{4}$ films ont he (100) and (111) oreientated silicon wafers were 0.5 - 0.6 $\mu$C/cm$^{2}$ and that of th efilms on the (110) wafers was 1.2$^{\circ}C$/cm$^{2}$.

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Synthesis of Zeolite Using Discharged Fly Ash in an Industrial Complex in Ulsan (울산지역 공단에서 발생되는 비산재를 이용한 제올라이트 합성)

  • Lee, Chang-Han;Park, Jong-Won
    • Journal of Korean Society of Environmental Engineers
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    • v.33 no.5
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    • pp.301-306
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    • 2011
  • In this study, zeolite was synthesized by hydrothermal, fusion, and fusion/hydrothermal methods with fly ash, coal fly ash, and a waste catalyst discharged from thermal power plants and incinerator in Ulsan area. Coal fly ashes (CFAs) and a waste catalyst containing amounts of $SiO_2$ and $Al_2O_3$ ranging from 60.29 to 89.62 wt%. CFAs were mainly composed of quartz and mullite which were assayed by a XRD pattern. Zeolite could be synthesized by CFAs and the waste catalyst when all methods were used. Na-A zeolite (Z-C1, Z-C2, and Z-W5) are mainly synthesized by the fusion method from CFAs and the waste catalyst. Z-C1 and Z-C2 formed by-products, calcite peaks, which is caused by the content of CaO in CFAs and the addition of $Na_2CO_3$ for a synthetic process.

Role of Berberis lycium in Reducing Serum Cholesterol in Broilers

  • Chand, N.;Durrani, F.R.;Qureshi, M.S.;Durrani, Z.
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.4
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    • pp.563-568
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    • 2007
  • This study was planned to investigate the role of Berberis lycium in reducing serum cholesterol in broilers. Six experimental rations designated as A, B, C, D, E and F having 0, 0.5, 1.0, 1.5, 2.0 and 2.5% Berberis lycium were fed to 240 broiler chicks, randomly distributed into 24 replicates, so as to have 4 replicates per group and 10 chicks per replicate. The experiment lasted for 35 days. Average serum total cholesterol, triglyceride, high density lipoprotein (HDL) and low density lipoprotein (LDL) were used as criteria of response. Average total serum cholesterol per chick was 129.33, 120.50, 116.50, 113.00, 101.67 and 114.00 mg/dl for group A, B, C, D, E and F respectively. Total serum cholesterol showed decreasing trend with the increasing level of Berberis lycium unto 2% (p<0.05). Mean serum triglyceride level per chick was 60.00, 58.17, 58.00, 55.33, 50.17 and 48.50 mg/dl for group A, B, C, D, E and F respectively. Mean serum triglyceride showed decreasing trend with the increasing level of Berberis lycium (p<0.05). Serum triglyceride was significantly lower in group F than other groups. Mean HDL per chick for the six experimental groups A, B, C, D, E and F was 52.08, 53.42, 60.42, 62.25, 62.92 and 54.50 mg/dl respectively. HDL showed increasing trend with the increase in the level of Berberis lycium unto 2%. The average serum LDL per chick was 65.25, 55.45, 44.48, 39.68, 28.72 and 49.80 mg/dl for group A, B, C, D, E and F respectively. LDL also showed decreasing trend with the increase in the level of Berberis lycium unto 2% (p<0.05). It was concluded that Berberis lycium added to feed at the rate of 2.0% can be used in broiler feed for reducing serum total cholesterol, triglyceride and LDL and increasing HDL.

Thermal Resistance and daf-16 Regulation of Fermented Zizyphus jujuba Fruits in Caenorhabditis elegans

  • Yu, Young-Bob
    • Korean Journal of Plant Resources
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    • v.33 no.6
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    • pp.645-650
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    • 2020
  • The mechanism of anti-aging of fermented jujube (Zizyphus jujuba fruits (ZJF)) was investigated using transgenic daf-16 and mev-1 strains of C. elegans. Jujube extracts fermented for 7 days (F7-ZJF) and 14 days (F14-ZJF) with Laetiporus sulphureus were treated to a NGM agar plate with 10-15 transgenic daf-16 and mev-1 strains of the synchronized age. There was no difference of lifespan between the drug-treated group (7-day fermented ex. (F7-zjf-200 ㎍/mL), 14-day fermented ex. (F14-zjf-200 ㎍/mL)) and the non-treatment group in both daf-16 and mev-1 strains. In the thermal stress experiment, F7-zjf-200 ㎍/mL showed a significant (t = 4.017) activity in thermal stress resistance with a 12% higher survival rate than the control group. In the survival test in H2O2, F7-zjf-200 ㎍/mL and F14-zjf-100 ㎍/mL have significant activity in oxidative stress resistance compared to the control group. This study indicates that life span expand of N2 strain of the jujube extract is related to the regulation of daf-16 and inhibition of mev-1 signal in C. elegans.

ON AN L-VERSION OF A PEXIDERIZED QUADRATIC FUNCTIONAL INEQUALITY

  • Chung, Jae-Young
    • Honam Mathematical Journal
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    • v.33 no.1
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    • pp.73-84
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    • 2011
  • Let f, g, h, k : $\mathbb{R}^n{\rightarrow}\mathbb{C}$ be locally integrable functions. We deal with the $L^{\infty}$-version of the Hyers-Ulam stability of the quadratic functional inequality and the Pexiderized quadratic functional inequality $${\parallel}f(x + y) + f(x - y) -2f(x) - 2f(y){\parallel}_{L^{\infty}(\mathbb{R}^n)}\leq\varepsilon$$ $${\parallel}f(x + y) + g(x - y) -2h(x) - 2f(y){\parallel}_{L^{\infty}(\mathbb{R}^n)}\leq\varepsilon$$ based on the concept of linear functionals on the space of smooth functions with compact support.

Synthesis, Structure and Thermal Properties of Bifurazano[3,4-b:3',4'-f]furoxano[3'',4''-d]oxacyclohetpatriene (BFFO)

  • Zhou, Yanshui;Xu, Kangzhen;Wang, Bozhou;Zhang, Hang;Qiu, Qianqian;Zhao, Fengqi
    • Bulletin of the Korean Chemical Society
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    • v.33 no.10
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    • pp.3317-3320
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    • 2012
  • A novel energetic compound, bifurazano[3,4-b:3',4'-f]furoxano[3'',4''-d] oxacyclohetpatriene (BFFO), was synthesized through special etherification and its structure was determined by single crystal X-ray diffraction. The crystal of $BFFO{\cdot}H_2O$ is monoclinic, space group P2(1)/c with crystal parameters of a = $9.324(4){\AA}$, b = $9.727(4){\AA}$, c = $10.391(4){\AA}$, ${\beta}=106.305(6)^{\circ}$, V = $904.5(6){\AA}^3$, Z = 4, ${\mu}=0.17mm^{-1}$, F(000) = 512 and $D_c=1.866g\;cm^{-3}$. Spectroscopic properties and thermal behaviors of BFFO were studied. BFFO presents good detonation properties.

Chemical Beam Deposition of $MgF_2$ Thin Films (화학선 증착법에 의한 $MgF_2$ 박막제조)

  • 박보현;백성기
    • Journal of the Korean Ceramic Society
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    • v.33 no.3
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    • pp.299-306
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    • 1996
  • We invesgated the fesibility of thin films deposition by pyrolysis of metalorganic precursors using chemical beam deposition (CBD) process. We attempted to understand the effects of deposition variables such as substrate temperature operating pressure effusion cell temperature and H2 partial pressure on the properties of MgF2 grown by CBD. Mg(tfac)2 was used as a precursor. MgF2 thin films were always grown in an amorphous state and crystallized bypost-annealing. he higher the substrate temperature and the lower the operating pressure the less the impurities I the deposited MgF2 thin films. H2 gas has to be supplied for the pyrolitic reaction of Mg(tfac)2 decomposition. MgF2 films annealed in H2 have lower C impurity than those annealed in O2. But their crysatllinity was independent of annealing atmosphere. The optimum conditions for the prepara-tion of MgF2 films by CBD process were as following : The substrate temperature 55$0^{\circ}C$ the operating pressure 10-4 torr; effusion cell temperature 21$0^{\circ}C$ the percentage of H2 100% Post-annealing in H2 gas was required to remove residual carbon and to form MgF2 crystalline phase.

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Study on the Quality of Market Dahi Compared to Laboratory Made Dahi

  • Shekh, A.L.;Wadud, A.;Islam, M.A.;Rahman, S.M.E.;Sarkar, M.M.;Ding, Tian;Choi, Jae-Ho;Oh, Deog-Hwan
    • Journal of Food Hygiene and Safety
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    • v.24 no.4
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    • pp.318-323
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    • 2009
  • Dahi is yoghurt like fermented dairy products available in Bangladesh made by traditional ways from raw milk. The aim of the present study was to check and compare the present quality status of the market dahi in reference to laboratory made dahi. For this, dahi was prepared in the laboratory (A) and collected from Kishoregonj district (B), Gazipur district (C), Bogra district (D) and Dhaka district (E and F). Consumer acceptance quality was judged on 100 point by a judge panel and chemical and microbiological quality test were done by established methods. 'A' type dahi was superior followed by E, D, F, B and C considering the consumer acceptance quality parameter. From chemical test, it appears that, B possess the lowest pH ($3.75\;{\pm}\;0.05$) and highest was in C ($4.46\;{\pm}\;0.15$). Total solids content of F type dahi ($318.40\;{\pm}\;4.44\;g/kg$) was highest and fat ($52.00\;{\pm}\;2.00\;g/kg$), protein ($44.33\;{\pm}\;2.00\;g/kg$) and ash ($10.76\;{\pm}\;0.31\;g/kg$) content were also higher in F type dahi. E and A type dahi were also with considerable figure in chemical quality parameter. Dahi B was inferior in respect of microbiological quality- total viable count (TVC) (${\times}\;10^5\;cfu/ml$) content was $94.00\;{\pm}\;4.58$, yeast (cfu/ml) content was $183.33\;{\pm}\;15.28$ and mold (cfu/ml) content was $53.33\;{\pm}\;15.28$. The TVC (${\times}\;10^5\;cfu/ml$), yeast (cfu/ml) and mold (cfu/ml) content of A type dahi were $39.67\;{\pm}\;4.51$, $50.00\;{\pm}\;10.00$ and $20.00\;{\pm}\;10.00$, respectively. Considering all the three major quality aspects dahi A was superior than others followed by F, D, E, C and B.