• Journal of Microbiology and Biotechnology
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• v.28 no.5
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• pp.707-717
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• 2018

Leukocytes are reportedly the first line of the innate immune defense and essential for the control of common bacterial infections. Therefore, in this work, the antibacterial activity of crocodile leukocyte extract against Propionibacterium acnes was evaluated, and we also characterized the related activity of skin infection. The leukocyte extract showed the minimum inhibitory concentration to be $100{\mu}g/ml$ to P. acnes. SEM imaging demonstrated that the leukocyte extract adversely affected P. acnes cell permeability in a concentration-dependent manner. Furthermore, the crocodile leukocyte extract could significantly reduce proinflammatory markers and decrease inflammatory signs in infected mouse ears. The crude leukocyte extract was further purified using FPLC and RP-HPLC. The resulting fraction F5 was indicated as the anti-acne peptide-containing fraction. The molecular mass of the peptide contained in F5 was calculated to be 4,790.5 Da. N-Terminal sequencing revealed the amino acid sequence as GPEPVPAIYQ, which displays similarities to immunoglobulin A and leucine-rich repeat neuronal protein. This is the first reported amino acid sequence of a crocodile leukocyte extract that possesses anti-acne activity. To attempt to use it in a prototype cosmetic, an anti-acne gel containing crude crocodile leukocyte extract was formulated, resulting in seven gel formulations (G1, G2, G3, G4, G5, G6, and G7). The formulations G5, G6, and G7 exhibited 2-fold higher anti-acne activity than G1-G4. Investigation of accelerating stability studies of anti-acne gel formulations G5, G6, and G7 demonstrated that a low storage temperature ($4^{\circ}C$) is suitable for maintaining the physical properties and biological activity of the anti-acne gel products.

• Radiation Oncology Journal
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• v.11 no.2
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• pp.397-401
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• 1993

We investigated the usefulness of tumor-associated antigen (TA-4) by a radioimmunoasay method in estimating the extent of disease and tracking the clinical course of disease in 58 patients with cervical cancer. According to our results and those of other authors, the normal range of serum TA-4 was arbitrarily taken to be less than 2 ng/ml. The proportion of the pretreatment positive serum TA-4 level of 48 squamous cell carcinoma patients was $60\%.$ And $40\%$ in 5 adenocarcinoma patients. Advanced disease group showed higher incidence of positive serum TA-4 level; $40\%,\;72\%,\;63\%,\;and\;100\%$ in stage I to IV, respectively. And the absolute values of TA-4 were higher in advanced disease. In patients treated with radiation, elevated serum TA-4 level usually declined after 3000 cGy and further dropped to normal range in $44\%$ after 5000 cGy. The positive rate in primary cervical cancer was $59\%,$ (32/54) and $100\%$ (4/4) in recurrent conical cancer. And 15 patients with recurrent or persistent disease during follow-up revealed $80\%$ positive serum TA-4 level. In conclusion, it would be suggested that serial serum TA-4 measurements may be helpful in tracking the clinical course during and after treatment.

• The Korea Journal of Herbology
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• v.38 no.5
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• pp.9-19
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• 2023

Objectives : Osteoporosis is a systemic skeletal disorder characterized by reduced bone mineral density and increased risk of fractures. Bisphosphonates and selective estrogen receptors, which are bone resorption inhibitors that are currently widely used as osteoporosis treatments, show serious side effects when administered for a long time. Research on bone resorption inhibitors that complement the problems of existing treatments is needed. The purpose of this study was to investigate the effect of inhibiting osteoclast differentiation and activity on the tuberous root of Ampelopsis japonica (Thunb.) Makino (AM). Methods : After extracting AM using distilled water and ethanol, the inhibitory effects of the two solvents on osteoclast differentiation were compared using the RANKL-induced in vitro experimental model and the TRAP assay kit. The impact of AM on bone resorption was investigated through the pit formation assay, and its effect on F-actin formation was assessed through fluorescent staining. Additionally, protein and mRNA expression levels of osteoclast differentiation markers (NFATc1, c-Fos, TRAP and ATP6v0d2) and resorption markers (MMP-9, CTK, and CA2) were analyzed via western blot and RT-PCR. Results : AM treatment significantly decreased the number of TRAP-positive cells and pit formation area. Furthermore, AM suppressed both the protein and mRNA expression of NFATc1 and c-Fos, key transcription factors involved in osteoclast differentiation and it downregulated the expression of osteoclast-associated genes such as TRAP, CTK, MMP-9, CA2, and ATP6v0d2. Conclusions : These results suggest that AM can inhibit bone resorption and osteoclast differentiation, indicating its potential for use in the treatment and prevention of osteoporosis.

• Women's Health Nursing
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• v.3 no.2
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• pp.208-223
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• 1997

This study was made to suggest the nursing strategies for promoting the behaviors about bone mass health behaviors in order to prevent middle aged women's osteoporosis. This study was a descriptive-correlational design that also concerned to the types which improve bone mass promoting behaviors by inspecting patterns of health locus of control method out of recognizable variables of health improving models influencing on these bone mass promoting behaviors. For these purpose, data were collected by self reported questionnaire in middle school, from 158 women living in Seoul. The measuring tools used in this study about bone mass promoting behaviors and multidimensional health locus of control, were developed by author on the basis of literature review and analyzed by SPSS-PC window, into pearson's correlation, ANOVA, multiple regression, cluster analysis. Data was analyzed as follows. 1. 6 Multidimensional health locus of control scale clusters were existed. : a)cluster I (pure internal), b)cluster II(pure chance), c) cluster III(Believer in control), d), cluster IV(Type VI), e)cluster V(yea sayer), f) cluster VI(nay sayer). There were no findings of the powerful others external cluster and double external cluster. 2. The higher the value of internal health locus of control was, the better the bone mass promoting behaviors were(r=.2891, $p=.00^{**}$). The higher the value of chance external health locus of control was, the worse the bone mass promoting behaviors were(r=-.1367, $p=.00^{**}$). 3. On the basis of these relationships, 6 clusters were significantly different in the bone mass promoting behaviors(F=2.27, $p=.05^*$). The value of bone mass promoting behaviors was ranked the order of type VI>believer in control>pure internal>yea sayer>nay sayer>pure chance external highly. 4. Bone mass promoting behaviors were not significantly different as to age. Suggestion. Based on the results from the study, I would like to make some suggestions as follows. 1) To delay the loss of bone mass in middle aged women, the study on the cluster of the multidimensional health locus of control should be conducted repeatedly. 2) The tool of multidimensional health locus of control should be developed through a qualitative method adjusted on Korean' health culture.

• Corrosion Science and Technology
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• v.6 no.4
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• pp.159-163
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• 2007

Micron size Co-alloy 800 (T800) powder is coated on the high temperature, oxidation and corrosion resistant super alloy Inconel 718 substrate by the optimal high velocity oxy-fuel (HVOF) thermal spray coating process developed by this laboratory. For the study of durability improvement of high speed spindle operating without lubricants, friction and sliding wear behaviors of the coatings are investigated both at room and at an elevated temperature of $1000^{\circ}F(538^{\circ}C)$. Friction coefficients, wear traces and wear debris of coatings are drastically reduced compared to those of non-coated surface of Inconel 718 substrate both at room temperature and at $538^{\circ}C$. Friction coefficients and wear traces of both coated and non-coated surfaces are drastically reduced at higher temperature of $538^{\circ}C$ compared with those at room temperature. At high temperature, the brittle oxides such as CoO, $Co_{3}O_{4}$, $MoO_2$ and $MoO_3$ are formed rapidly on the sliding surfaces, and the brittle oxide phases are easily attrited by reciprocating slides at high temperature through oxidation and abrasive wear mechanisms. The brittle solid oxide particles, softens, melts and partial-melts play roles as solid and liquid lubricants reducing friction coefficient and wear. These show that the coating is highly recommendable for the durability improvement coating on the machine component surfaces vulnerable to frictional heat and wear.

• Korean Journal of Environmental Biology
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• v.37 no.4
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• pp.526-534
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• 2019

Chlorella gloriosa (Chlorellaceae, Trebouxiophyceae) was isolated from seawater off the coast of the Dokdo Islands in Korea. An axenic culture was established using the streak-plate method on f/2 agar media supplemented with antibiotics, allowing identification of the isolate by morphological, molecular, and physiological analyses. The morphological characteristics observed by light and electron microscopy revealed typical morphologies of C. gloriosa species. The molecular phylogenetic inference drawn from the small-subunit 18S rRNA sequence verified that the microalgal strain belongs to C. gloriosa. Additionally, gas chromatography-mass spectrometry analysis showed that the isolate was rich in nutritionally important omega-3 and -6 polyunsaturated fatty acids and high-performance liquid chromatography analysis revealed that the high-value antioxidants lutein and violaxanthin were biosynthesized as accessory pigments by this microalga, with arabinose, galactose, and glucose as the major monosaccharides. Therefore, in this study, a Korean marine C. gloriosa species was discovered, characterized, and described, and subsequently added to the national culture collection.

• Korean Journal of Materials Research
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• v.6 no.8
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• pp.841-851
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• 1996

In a recent investigation, the formation of bainite phase in direct-quenched low carbon non heat-treated steel was reported. In this study the effects of bainite phase on the mechanical properties of direct-quenched microalloying steels were investigated. By isothermal transformation at $480^{\circ}C$ for 7 sec., volume fraction of bainite lath was 15~20%, and the UTS and impact energy were increased. In this case $B_{ll}$ and $B_{lll}$ type bainite was observed and the fractography of impact test specimen showed a ductile fracture tendency. Isothermal transformation for 100sec., yielded 30% volume fraction of granular bainite and the mechanical properties were decreased. The f ractography of impact test specimen showed a brittle fracture tendency. The addition of Mo was more effective than B for improving impact energy because amounts of boron aditions were restricted to considerably lower levels, typically 10~ 30ppm. From this study, it is predicted that 15~20% volume fraction of lath bainite on the direct quenching process is procduced by addition of Mo up to 1.2wt. % and controlling the finish forging proc¬ess at $1000^{\circ}C$ and using oil as direct quenching media. This will improve mechanical properties of the direct- quenched steel.

• Membrane Journal
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• v.13 no.2
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• pp.65-72
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• 2003

This study was focused on the investigation of filtration characteristics of membrane-coupled fermentor system for dissolved organics recovery from liquid organic sludge. On the filterability of MF over the range of $0.1{\sim}5 {\mu}m,$ the magnitude of total membrane resistance ($R_t$) is ranged as follows in the order; $0.1 {\mu}m>0.2{\mu}m>0.5 >1{\mu}m>2{\mu}m>5{\mu}m$. The cake layer resistance ($R_c$) occupied about 68~88% of total resistance with fermented sludge. Permeation flux decline was mainly attributed to the $R_c$, which was formed by a strong deposition from physico-chemical interactions of solids on membrane surface. Higher suspended solids (SS) concentration of suspension caused lower permeation flux. However, there was not a proportion relation beyond a certain SS concentration. The cross-flow velocity on the membrane surface was faster, which resulted in the higher permeation flux and also more efficient with low trans membrane pressure (TMP) in viewpoint of energy efficiency. The appropriate pH of suspension was over the range of 5.0~6.0 for dissolved organics recovery as well as the permeation flux. It is possible f3r bacteria to be separated perfectly with $0.1{\mu}m\; and \;0.2{\mu}m$ membrane pore size. Based on experimental results, most appropriate membrane pore size for the recovery is believed to around $1{\mu}m$.

• Parasites, Hosts and Diseases
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• v.28 no.2
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• pp.91-100
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• 1990

Paragonimus westermani is a tissue migrating parasite in the early stage until arriving at lung, and most of the parasites spend their life spans there. Considerable immune responses including activation of macrophages are taken place during the residence of parasites in the host. However, concerning the immunologic defense mechanisms of the host against this parasite, only a few document is available so far. In this study, the cytotoxic effect of peritoneal macrophages under the presence of antibody and/or complement against metacercariae of F. westermani was investigated in vitro. Metacercarlae were collected from the crayfish, Cambaroides similis and hatched out in Tyrode solution (pH 7.4). Plastic adherent cells from normal or infected rat (Wistar) peritoneal exudates were used as experimental macrophages. Polyclonal antibodies were obtained from infected rats and a cat. Cat IgG was fractioned with ion exchange chromatography. Fresh rabbit complement was used according to experimental scheme. Various combinations of peritoneal macrophages, normal or infected rat serum, complement and cat IgG were incubated at $36^{\circ}C$ in 5% $CO_2$ incubator for 6, 14, 24 and 48 hours. The results obtained were as follows: 1. P. westermani infection activated peritoneal macrophages non-specifically and this activation induced increases of cell adherence and cytotoxicity on metacercariae. 2. In the presence of infected rat serum the antibody.dependent cell-mediated cytotoxicity of peritoneal macrophages on metacercariae was significantly increased and showed a peak at 6-hour incubation. But the cytotoxic effect was markedly reduced after inactivation of complement and heat.labile IgE antibody by the heating of infected serum at 56$^{\circ}C$ for 30 minutes. 3. The highest cytotoxic effect (100%) of concomitant incubation with IgG and complement showed 24 hours after incubation, although cell adherence was relatively low at 6-hour incubation and 0% at 24-hour incubation. 4. Coordinative functions of complement with serum and IgG were effective in cell adherence and in cytotoxicity, but it is not clear the independent role of complement on the macrophage- mediated cytotoxicity in this study- With these results it is assumed that P. westermani infection can induce the non-specific activation of peritoneal macrophages, and strum antibodies including IgE antibody might enhance the cytotoxicity by macrophages,

• Parasites, Hosts and Diseases
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• v.49 no.4
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• pp.427-430
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• 2011

The aim of this study was to test the susceptibility of mice to Trypanosoma evansi treated with human plasma containing different concentrations of apolipoprotein L-1 (APOL1). For this experiment, a strain of T. evansi and human plasma (plasmas 1, 2, and 3) from 3 adult males clinically healthy were used. In vivo test used 50 mice divided in 5 groups (A to E) with 10 animals in each group. Animals of groups B to E were infected, and then treated with 0.2 ml of human plasma in the following outline: negative control (A), positive control (B), treatment with plasma 1 (C), treatment with plasma 2(D), and treatment with plasma 3 (E). Mice treated with human plasma showed an increase in longevity of $40.9{\pm}0.3$ (C), $20{\pm}9.0$ (D) and $35.6{\pm}9.3$ (E) days compared to the control group (B) which was $4.3{\pm}0.5$ days. The number of surviving mice and free of the parasite (blood smear and PCR negative) at the end of the experiment was 90%, 0%, and 60% for groups C, D, and E, respectively. The quantification of APOL1 was performed due to the large difference in the treatments that differed in the source plasma. In plasmas 1, 2, and 3 was detected the concentration of 194, 99, and 115 mg/dl of APOL1, respectively. However, we believe that this difference in the treatment efficiency is related to the level of APOL1 in plasmas.