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Efficacy of Brucella abortus strain RB51 vaccine in Korean mongrel dogs against virulent strains of B. abortus biotype 1 and B. canis

  • Hur, Jin;Baek, Byeong-Kirl
    • Korean Journal of Veterinary Service
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    • v.33 no.1
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    • pp.29-35
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    • 2010
  • This study was performed to test the hypothesis that Brucella abortus strain RB51 (SRB51) might protect Korean indigenous mongrel dog against challenge with either virulent B. abortus biotype 1 or B. canis. A total of 12 Korean mongrel dogs were divided into four groups (Group A, B, C and D). Dogs belonging to Group A and C were inoculated subcutaneously with $1{\times}10^9$ CFU of SRB51 in 1ml of sterile phosphate buffered saline (PBS). Dogs of Group B and D were inoculated subcutaneously with 1ml of sterile PBS as control. At 12 weeks post vaccination, dogs of Group A and B were challenged by oral inoculation of virulent strain of B. canis ($5.0{\times}10^9$ CFU) and dogs of Group C and D were challenged by oral inoculation of virulent strain of B. abortus biotype 1 ($4.4{\times}10^{10}$ CFU). The serum antibodies titers in all dogs were monitored at regular interval for eight weeks after challenge (AC) by standard tube agglutination test, plate agglutination test, rose bengal test, 2-mercaptoethanol rapid slide agglutination test and 2-mercaptoethanol tube agglutination test. No antibody titers in Group A and C was detected. Also, the challenge strains were not found from blood of all dogs of Group A and C from 1 week AC till the end of the experiment by culture and modified AMOS-PCR, whereas B. canis and B. abortus challenge strains were detected from blood of Group B and D, respectively. In addition, neither of two challenge bacteria was recovered from liver, spleen, kidneys, lymph nodes and reproductive tracts of Group A and C dogs after postmortem. However, B. canis and B. abortus challenge strains were isolated from these tissues of Group B and D, respectively. These data suggest that SRB51 could be a promising vaccine candidate for immunizing dogs to control canine brucellosis caused by B. canis or B. abortus.

A Study of Fabrication and Strengthening of Plate Girder (판항교제작(鈑桁橋製作) 및 보강(補强)에 관한 연구(硏究))

  • Suh, Young Kap
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.3 no.3
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    • pp.39-51
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    • 1983
  • Increasing of load on the highway bridge necessitate the strengthening of load capacity of bridge by some method. The method of strengthening is the usage of pre-stressing high tensile steel line. Having finished pre-stressing work, line is anchored both end, then it composed a member of bridge structure when loading. This paper includes the method and mechanism of strengthening of I-beam span(same originality of plate girder), could be summerized as following; (a) Simple girder, 2 span and 3 span continuous girder increasing the load capacity by more than 80 % for concentrated load. (b) For uniformly distributed load, when all span loaded, load capacity is increase more than 80% to 100% except 3 span continuous.

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Effect of Working Fluids on the Thermal Behavior of a Bi-directional Solar Thermal Diode (작동유체가 양방향성 태양열 열다이오드의 열성능 변화에 미치는 영향 분석)

  • Ko, Yung-Joo;Lee, Heon-Ju;Chun, Won-Gee;Chen, Kuan;Lim, Sang-Hoon
    • Journal of the Korean Solar Energy Society
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    • v.28 no.1
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    • pp.9-17
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    • 2008
  • An experimental investigation has been carried out to study the effects of different working fluids on the behavior and thermal performance of a hi-directional thermodiode. The thermodiode was made up of two rectangular loops mounted between a collector plate and a radiator plate. Rotatable joints between the horizontal and inclined segments of the loops enable easy alteration of the direction of heat transfer. The loops and the tank were filled with a working fluid for effective heat transfer when the thermodiode was forwarded biased. Six different working fluids were tested with thermal conductivity values ranging from 0.1 to $0.56W/m-^{\circ}C$, thermal expansion coefficient values ranging from $1.8\;{\times}10^{-4}$ to $1.3\;{\times}\;10^{-3}\;K^{-1}$, and kinematic viscosity values ranging from $0.65\;{\times}\;10^{-6}$ to $100\;{\times}\;10^{-6}\;m^2/s$. Especially, mixtures of $Al_2O_3$ (30nm Particle) in deionized water have been tested for the volumetric ratios of 0.01, 0.02, 0.03, 0.1, 0.2%. Each experiment was carried out after the loop was filled with a working fluid for effective heat transfer and the thermodiode was forwarded biased. The solar thermodiode was heated by a radiant heater consisting of 20 halogen lamps that generated a heat flux of about $1000\;W/m^2$ on the collector surface. Results are given in terms of temperature development in different parts of the loop as heat is delivered from its hot end to the surrounding atmosphere by the radiator made of copper plates.

An Estimation of the Fatigue Behavior on the Cruciform Type Specimen by Variation of the Stress Ratio (응력비 변화에 따른 십자형 접합부의 피로거동 평가)

  • 김태봉;서상구;우상익
    • Journal of the Korean Society of Safety
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    • v.15 no.1
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    • pp.140-145
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    • 2000
  • This paper was composed with fatigue test of the cruciform specimens, as load carrying and non-load carrying type. It also has performed computational analyses for geometric condition of the fillet welding bead. As test results, the effect of stress ratio in the specimen was insignificant. Stress ranges were varied with R=0.1~0.2. The fatigue cracks that were found in the load carrying type specimens and most specimens welded with contact were developed at the end of welds. The fatigue strength of specimen that have fractured in maternal plate was found about ${\Delta}\sigma_c$=63.5MPa. It's about 24% less than that of the non-load carrying type specimens having about ${\Delta}\sigma_c$=83.8MPa. A category of the Fatigue design specifications which provide for cruciform details was defined grade C as a stress of the maternal member. And then, the fatigue strength to be transformed into the maternal stress was found about 78.27 MPa, it tends to be less than that of allowable fatigue strength.

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Development of the Ag/Cu Ingots for Mokumegane Jewelry (모꾸메가네 장신구를 위한 은/동 접합 잉곳 소재 개발)

  • Song, Oh-Sung;Kim, Jong-Ryul;Kim, Myung-Ro
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.9 no.1
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    • pp.9-15
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    • 2008
  • Mokumegane is one of the sophisticated metal craft techniques enabling wood grain surface effect. To embody the mokumegane, an ingot of well-bonded stacked metal plates has been required. Traditionally prepared mokumegane ingots were bonded using charcoal which enables reduction atmosphere, but sometimes end up with collapse of bonding interface due to the lack of reliable process control. We proposed a systematic vacuum direct bonding process for ingots. First, we confirmed copper//copper homogeneous plate bonding at $900^{\circ}C$ by applying uniaxial press of 2.5kg. We observed 80min required to obtain 90%-bonding ratio and the diffusion coefficient would be enhanced up to 100 times due to surface effect. Second, by considering enhanced diffusion behavior, we also obtained optimum bonding condition in copper/silver heterogeneous plates that ensures 90%-bonding ratio at $700^{\circ}C$ for 10min with apply uniaxial press. A 7-layered copper/silver ingot is prepared successfully, and eventually the prototype mokumegane cases for mobile phone were fabricated with these ingot.

Evaluation of the Microbiological Quality of Glazed Spanish Mackerel in Teriyaki Sauce by Adding Lemon Juice Using Sous Vide Cook-chill Method (Sous Vide Cook-chill 법으로 생산된 삼치 데리야끼 조림의 레몬즙 첨가에 따른 미생물적 품질평가)

  • Kim, Heh-Young;Ko, Sung-Hee;Lee, Kyung-Yeoun
    • Korean journal of food and cookery science
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    • v.24 no.5
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    • pp.713-721
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    • 2008
  • The principal objective of this study was to evaluate the microbiological quality of Glazed Spanish Mackerel in Teriyaki sauce by adding Lemon juice, using a Sous vide Cook-chill method. The control was prepared by mixing 100 g of cut Spanish Mackerel and 15 mL of Teriyaki sauce. 1.5 mL lemon juice was added to 15 mL of Teriyaki sauce. The product was stored at below $3^{\circ}C$, and its microbiological quality was assessed over varying storage periods (0, 5, 10, 15 days). Total Plate Counts at product flow showed no significant difference between the control and the lemon juice-added sample. However, the coliform counts at the end of mixing each sauce were detected at low levels of 1.65 Log CFU/g in the lemon juice-added sample, as opposed to 2.63 Log CFU/g in the controls (p<0.05). The microbiological quality by storage day was relatively high in the lemon juice-added samples. After reheating, the Total Plate Counts and Coliform counts of the lemon juice-added samples satisfied the standard applied in the processed food (5.0 and 2.0 Log CFU/g, respectively). It can be concluded that the addition of Lemon juice was more effective in ensuring the safety and extending the shelf life of Sous vide Glazed Spanish Mackerel in Teriyaki sauce.

Changes in Quality Characteristics of Vacuum-Shrink Packaged Bierschinken after Stuffing in Fibrous Casing during Chilled Storage (화이브러스 케이싱에 충전 후 진공 수축 포장한 비어슁켄의 냉장 중 품질 특성 변화)

  • Lee Jung-Pyo;Lee Keun-Taik
    • Food Science of Animal Resources
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    • v.25 no.2
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    • pp.121-126
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    • 2005
  • The changes in quality characteristics and shelf-life of vacuum-shrink packaged Bierschinken after stuffing in fibrous casing and stored at 5 and $10^{\circ}C$ were investigated The total aerobic plate count (APC) of Bierschinken was initially <2.00 $log_{10}CFU/cm^2$, however increased gradually over storage period The APCs of Bierschinken maintained at the level of 4.86 and 5.13 $log_{10}CFU/cm^2$ after 35 days at 5 and $10^{\circ}C$, respectively. pH tended to decrease with the extension of storage period The values of TBA and VBN tended to increase with storage time, of which trend was pronounced at $10^{\circ}C$ rather than at $5^{\circ}C$. According to the sensory evaluation, Bierschinken had the marketing value till 28 days irrespective of storage temperature. The shelf-life of Bierschinken tested might be preferentially restricted by the occurrence of off-odor and off-flavor at the end of storage period.

The effect of whole body vibration training on postural sway in patients with spinal cord injury: a pilot study

  • Asakawa, Yasuyoshi;Lee, Myung-Mo;Song, Chang-Ho
    • Physical Therapy Rehabilitation Science
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    • v.2 no.2
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    • pp.70-74
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    • 2013
  • Objective: The aim of this study was to determine the impact of the application of whole body vibration training (WBV) on the balance ability of patients with an American Spinal Injury Association (ASIA) type C or D spinal cord injury. Design: Randomized controlled trial. Methods: Twelve patients with spinal cord injury were enrolled in this study. The participants were randomized to an experimental group (n=6) or control group (n=6). The subjects in the experimental group received WBV exercise and the control group received the sham exercise without vibration. The vibrations were adjusted vertically to the patient at a 30 Hz frequency and 3 mm amplitude. The whole body vibration lasted for 16 minutes in total including 5-minutes warm-up and cool-down at the beginning and end of the program, respectively. The static sitting balance ability was assessed by measuring the postural sway while sitting on the force plate with the eyes opened or closed. Postural sway length was measured for 30 seconds with a self-selected comfortable position. Results: In the static balance test, the anterio-posterior, medio-lateral, and total postural sway length with the eyes open and closed was improved significantly before and after the intervention in the experimental group (p<0.05). The experimental group showed significantly more improvement than the control group (p<0.05). Conclusions: Our results demonstrated that WBV training has a positive effect on improving static sitting balance and enhanced control of postural sway in patients with an ASIA-C or D type spinal cord injury.

Changes of Particle Filtration Efficiency of Cloth Masks by Machine Washing and Cloth Expansion (세탁 및 옷감 신장에 따른 천마스크 제품의 여과효율 변화)

  • Kim, Seung Won
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.27 no.2
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    • pp.115-122
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    • 2017
  • Objectives: This study was designed to evaluate the changes in the particle filtration efficiencies of five cloth masks (4 plate types, 1 cup type) with an increasing number of machine washings and the degree of cloth expansion. Methods: NaCl aerosols were generated using an atomizer and passed through cloth masks in a dynamic aerosol chamber. Particle concentrations were measured both before and after for the cloth masks using an optical particle counter (OPC) in the size range of $0.3{\sim}10{\mu}m$. Results: In the original condition, the filtration efficiencies of the five cloth masks were A: 20.1%, B:30.9%, C: 25.0%, D: 26.5%, and E: 40.9%. As the number of washings increased in the order of one, two, and four times, the filtration efficiencies of cloth mask C increased. The filtration efficiency of A, D, and E increased after the first washing. With the exception of B, the filtration efficiency of cloth masks increased after the second washing and those of all cloth masks increased after the fourth washing. This might be caused by the fibers untangling from the yarn and being freed at one end. In this status, the packing density of the textile will not change, but the distances between fibers will increase, which might bring about an increase in filtration efficiency. When the cloth masks were extended by 10% and 20% in one direction, the filtration efficiencies of cloth masks B, D, and E decreased at 10% extension, and those of all cloth masks decreased at 20% extension. When the cloth masks were expanded by 10% and 20% in two directions, the filtration efficiencies of all cloth masks decreased by at least 34.7% at 10% extension, and by at least 60.9% at 20% extension Conclusions: The filtration efficiency of cloth masks could decrease after one to two machine washings, but will increase after four washings in comparison with their original performances. The filtration efficiency of cloth masks will decrease when they are expanded, such as when stretching over the nose during wearing status.

USE OF ACRYLIC RESIN IN RELEASING CHLORHEXIDINE (아크릴릭 레진상을 이용한 클로르헥시딘의 방출에 관한 연구)

  • Lee, Eun-Young;Choi, Yeong-Chul
    • Journal of the korean academy of Pediatric Dentistry
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    • v.25 no.4
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    • pp.797-810
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    • 1998
  • The purpose of this study was to develop a new way of delivery system of chlorhexidine using self-curing acrylic resin. Different preparations of chlorhexidine, such as chlorhexidine varnish($Chlorzoin^{(R)}$) and chlorhexidine diacetate crystalline, were mixed into self-curing acrylic resin with different methods. Every resin plate was made and was immersed in 100ml of distilled water individually, and kept in an incubator at $37^{\circ}C$. Solution(0.8ml) was collected from the each container at every 24 hours, and the amount of released chlorhexidine in the solution was measured in an ultraviolet spectrophotometer at 255nm. Flexural strength of all of the resin plates in the Experiment 2-A and 2-B were measured using Instron at the end of the experimental periods. The results were as follows: 1. It was found that chlorhexidine was released from the experimental groups in the Experiment 1, 2-A, and 2-B. And the release of chlorhexidine from all of the experimental groups showed a pattern of sustained-release preparation. 2. It seemed likely that a condition of "dryness" reduced a release of chlorhexidine from the chlorhexidine varnish. 3. It may be stated that a method of "chlorhexidine diacetate mix" with the polymer be more efficient than a method of "Chlorzoin mix" with the monomer. 4. Although it was evident that a flexural strength of the acrylic resin plates be reduced by a mix of either Chlorzoin or chlorhexidine diacetate crystalline, it seemed likely that the resin plates except Group 4 and 5 in the Experiment 2-B may be usable in the clinical situation.

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