• Applied Biological Chemistry
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• 제12권
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• pp.33-41
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• 1969

1. Cheatomium globosum의 밀기울 배양기에서 조효소를 추출하고 황산암모니움 염석 부분을 cellulose 분말 column으로 2개의 cellulase활성 부분(C-1, C-2)을 분리 하였다. 그 하나는 환원당 증가 활성이 강하며 (C-1) 다른 하나는 곁도 감소 활성이 강하였다. (C-2) 그러나 단백질 량은 C-1부분이 많았고 C-2 부분은 적었다. 2. 환원당 증가 활성이 강한부분 (C-1)을 DEAE-Sephadex A-25 column에서 분리할 결과 다시 2개의 성분 (C-1-1 및 C-1-2)으로 나누어 졌다. 그리고 C-1-2는 column에 강하게 흡착되었고 2M-NaCl의 용액으로 용출되었다. 이는 착색 된 것으로 봐서 C-1-1과는 아주 다른 단백질로 생각이 된다. 3. Cellulase C-1-1을 다시 Amberlite XE-64 column으로 분별하여 단일의 peak를 얻었다. 4. Cellulase C-1-1 부분의 초원심 침강계 면은 단일의 peak로 나타나고 또 자외선 홍수 spectrum도 전형적인 단백질의 흡수 spectrum을 나타 내었다. 5. Cellulase C-1-1의 최적 pH는 환원당 증가활성법으로나 점도 감소 활성법으로 다 같이 pH 4.0이였다. 6. 그리고 그의 최적 온도는 $40^{\circ}C$였다. 7. Cellulase C-1-1의 pH 안정성은 $40^{\circ}C$에서 pH 5.0 내지 pH 8.0의 범위 내였다. 8. 그리고 열안정성은 pH 4.0에서 $50^{\circ}C$ 이하였다

• 한국응용과학기술학회지
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• 제18권3호
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• pp.214-232
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• 2001

CTA ester bonds in TG molecules were not attacked by pancreatic lipase and lipases produced by microbes such as Candida cylindracea, Chromobacterium viscosum, Geotricum candidium, Pseudomonas fluorescens, Rhizophus delemar, R. arrhizus and Mucor miehei. An aliquot of total TG of all the seed oils and each TG fraction of the oils collected from HPLC runs were deuterated prior to partial hydrolysis with Grignard reagent, because CTA molecule was destroyed with treatment of Grignard reagent. Deuterated TG (dTG) was hydrolyzed partially to a mixture of deuterated diacylglycerols (dDG), which were subsequently reacted with (S)-(+)-1-(1-naphthyl)ethyl isocyanate to derivatize into dDG-NEUs. Purified dDG-NEUs were resolved into 1, 3-, 1, 2- and 2, 3-dDG-NEU on silica columns in tandem of HPLC using a solvent of 0.4% propan-1-o1 (containing 2% water)-hexane. An aliquot of each dDG-NEU fraction was hydrolyzed and (fatty acid-PFB ester). These derivatives showed a diagnostic carboxylate ion, $(M-1)^{-}$, as parent peak and a minor peak at m/z 196 $(PFB-CH_{3})^{-}$ on NICI mass spectra. In the mass spectra of the fatty acid-PFB esters of dTGs derived from the seed oils of T. kilirowii and M. charantia, peaks at m/z 285, 287, 289 and 317 were observed, which corresponded to $(M-1)^{-}$ of deuterized oleic acid ($d_{2}-C_{18:0}$), linoleic acid ($d_{4}-C_{18:0}$), punicic acid ($d_{6}-C_{18:0}$) and eicosamonoenoic acid ($d_{2}-C_{20:0}$), respectively. Fatty acid compositions of deuterized total TG of each oil measured by relative intensities of $(M-1)^-$ ion peaks were similar with those of intact TG of the oils by GLC. The composition of fatty acid-PFB esters of total dTG derived from the seed oils of T. kilirowii are as follows; $C_{16:0}$, 4.6 mole % (4.8 mole %, intact TG by GLC), $C_{18:0}$, 3.0 mole % (3.1 mole %), $d_{2}C_{18:0}$, 11.9 mole % (12.5 mole %, sum of $C_{18:1{\omega}9}$ and $C_{18:1{\omega}7}$), $d_{4}-C_{18:0}$, 39.3 mole % (38.9 mole %, sum of $C_{18:2{\omega}6}$ and its isomer), $d_{6}-C_{18:0}$, 41.1 mole % (40.5 mole %, sum of $C_{18:3\;9c,11t,13c}$, $C_{18:3\;9c,11t,13r}$ and $C_{18:3\;9t,11t,13c}$), $d_{2}-C_{20:0}$, 0.1 mole % (0.2 mole % of $C_{20:1{\omega}9}$). In total dTG derived from the seed oils of M. charantia, the fatty acid components are $C_{16:0}$, 1.5 mole % (1.8 mole %, intact TG by GLC), $C_{18:0}$, 12.0 mole % (12.3 mole %), $d_{2}-C_{18:0}$, 16.9 mole % (17.4 mole %, sum of $C_{18:1{\omega}9}$), $d_{4}-C_{18:0}$, 11.0 mole % (10.6 mole %, sum of $C_{18:2{\omega}6}$), $d_{6}-C_{18:0}$, 58.6 mole % (57.5 mole %, sum of $C_{18:3\;9c,11t,13t}$ and $C_{18:3\;9c,11t,13c}$). In the case of Aleurites fordii, $C_{16:0}$; 2.2 mole % (2.4 mole %, intact TG by GLC), $C_{18:0}$; 1.7 mole % (1.7 mole %), $d_{2}-C_{18:0}$; 5.5 mole % (5.4 mole %, sum of $C_{18:1{\omega}9}$), $d_{4}-C_{18:0}$ ; 8.3 mole % (8.5 mole %, sum of $C_{18:2{\omega}6}$), $d_{6}-C_{18:0}$; 82.0 mole % (81.2 mole %, sum of $C_{18:3\;9c,11t,13t}$ and $C_{18:3 9c,11t,13c})$. In the stereospecific analysis of fatty acid distribution in the TG species of the seed oils of T. kilirowii, $C_{18:3\;9c,11t,13r}$ and $C_{18:2{\omega}6}$ were mainly located at sn-2 and sn-3 position, while saturated acids were usually present at sn-1 position. And the major molecular species of $(C_{18:2{\omega}6})(C_{18:3\;9c,11t,13c})_{2}$ and $(C_{18:1{\omega}9})(C_{18:2{\omega}6})(C_{18:3\;9c,11t,13c})$ were predominantly composed of the stereoisomer of $sn-1-C_{18:2{\omega}6}$, $sn-2-C_{18:3\;9c,11t,13c}$, $sn-3-C_{18:3\;9c,11t,13c}$, and $sn-1-C_{18:1{\omega}9}$, $sn-2-C_{18:2{\omega}6}$, $sn-3-C_{18:3\;9c,11t,13c}$, respectively, and the minor TG species of $(C_{18:2{\omega}6})_{2}(C_{18:3\;9c,11t,13c})$ and $ (C_{16:0})(C_{18:3\;9c,11t,13c})_{2}$ mainly comprised the stereoisomer of $sn-1-C_{18:2{\omega}6}$, $sn-2-C_{18:2{\omega}6}$, $sn-3-C_{18:3\;9c,11t,13c}$ and $sn-1-C_{16:0}$, $sn-2-C_{18:3\;9c,11t,13c}$, $sn-3-C_{18:3\;9c,11t,13c}$. The TG of the seed oils of Momordica charantia showed that most of CTA, $C_{18:3\;9c,11t,13r}$, occurred at sn-3 position, and $C_{18:2{\omega}6}$ was concentrated at sn-1 and sn-2 compared to sn-3. Main TG species of $(C_{18:1{\omega}9})(C_{18:3\;9c,11t,13t})_{2}$ and $(C_{18:0})(C_{18:3\;9c,11t,13t})_{2}$ were consisted of the stereoisomer of $sn-1-C_{18:1{\omega}9}$, $sn-2-C_{18:3\;9c,11t,13t}$, $sn-3-C_{18:3\;9c,11t,13t}$ and $sn-1-C_{18:0}$, $sn-2-C_{18:3\;9c,11t,13t}$, $sn-3-C_{18:3\;9c,11t,13t}$, respectively, and minor TG species of $(C_{18:2{\omega}6})(C_{18:3\;9c,11t,13c})_{2}$ and $(C_{18:1{\omega}9})(C_{18:2{\omega}6})(C_{18:3\;9c,11t,13c})$ contained mostly $sn-1-C_{18:2{\omega6}$, $sn-2-C_{18:3\;9c,11t,13t}$, $sn-3-C_{18:3\;9c,11t,13t}$ and $sn-1-C_{18:1{\omega}9}$, $sn-2-C_{18:2{\omega}6}$, $sn-3-C_{18:3\;9c,11t,13t}$. The TG fraction of the seed oils of Aleurites fordii was mostly occupied with simple TG species of $(C_{18:3\;9c,11t,13t})_{3}$, along with minor species of $(C_{18:2{\omega}6})(C_{18:3\;9c,11t,13t})_{2}$, $(C_{18:1{\omega}9})(C_{18:3\;9c,11t,13t})_{2}$ and $(C_{16:0})(C_{18:3\;9c,11t,13t})$. The sterospecific species of $sn-1-C_{18:2{\omega}6}$, $sn-2-C_{18:3\;9c,11t,13t}$, sn-3-C_{18:3\;9c,11t,13t}$, $sn-1-C_{18:1{\omega}9}$, $sn-2-C_{18:3\;9c,11t,13t}$, $sn-3-C_{18:3\;9c,11t,13t}$ and $sn-1-C_{16;0}$, $sn-2-C_{18:3\;9c,11t,13t}$, $sn-3-C_{18:3\;9c,11t,13t}$ are the main stereoisomers for the species of $(C_{18:2{\omega}6})(C_{18:3\;9c,11t,13t})_2$, $(C_{18:1{\omega}9})(C_{18:3\;9c,11t,13t})_{2}$ and $(C_{16:0})(C_{18:3\;9c,11t,13t})$, respectively.

• 한국수산과학회지
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• 제14권1호
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• pp.1-6
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• 1981

유지의 triglyceride 조성을 밝히기 위하여 비교적 구성지방산의 종류가 간단한 수종의 식물유를 시료로 하여 연구 검토하였는데, 본 보고에서는 참기름의 triglyceride 조성에 대하여 보고하기로 한다. 시료유를 Bio-Beads SX-2및 sephadex LH-20을 사용한 column chromatography에 의하여 triglyceride를 분리하고, 이것을 $\mu-Bondapak\;C_{18}$ column을 사용한 HPLC에 걸어 PN별로 triglyceride를 분획하여 분취하였다. 분취한 획분의 일부를 GLC에 걸어 acyl 탄소수별로 분획하고, 또 일부는methyl 화하여 GLC에 걸어 지방산조성을 분석하였다. 이들 결과로부터 triglyceride조성을 산정하였는데 산정할 수 있었는 triglyceride는 21종류이며, 그 중 주요 triglyceride는 $(2{\times}_{C18:1},\;C_{18:2};\;17.1\%),\;(C_{18:1},\;2{\times}C_{18:2};\;17.0\%),\;(3{\times}C18:2;17.0\%),$ $(3{\times}C_{18:1}; \;10.9\%),\;(3{\times}C_{18:2};\;9.6\%),$ $(C_{16:0},\;C_{18:1},\;C_{18:2};\;7.9\%),\;(C_{16:0},\;2{\times}C_{18:1};\;7.4\%),$ $(C_{16:0},\;2{\times}C_{18:2};\;6.8\%), (C_{18:0},\;C_{18:1},\;C_{18:2};\;3.1\%),$ $(2{\times}C_{18:0},\;C_{18:2};\;1.5\%),\;(C_{18:0},\;2{\times}C_{18:1};\;1.4\%),$ $(C_{16:0},\;C_{18:0},\;C_{18:1};\;1.3\%),\;(2{\times}C_{16:0},\;C_{18:1};\;1.2\%)$ 및 $(C_{16:0},\;C_{18:0},\;C_{18:2};\;1.0\%)$ 등이었다.

• Asian Pacific Journal of Cancer Prevention
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• 제13권10호
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• pp.4915-4921
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• 2012

Published data on any association between the CYP2E1 RsaI/PstI (c1/c2) polymorphism and liver cancer risk among east Asians are inconclusive. The aim of this Human Genome Epidemiology (HuGE) review and meta-analysis was to derive a more precise estimation of the relationship. A literature search of Pubmed, Embase, Web of science and CBM databases from inception through July 2012 was conducted. Twelve case-control studies were included with a total of 1,552 liver cancer cases and 1,763 healthy controls. Crude odds ratios (ORs) with 95% confidence intervals (CIs) were used to assess the strength of association under five genetic models. When all the eligible studies were pooled into the meta-analysis, the results showed that the c2 allele and the c2 carrier (c2/c2 + c2/c1) of RsaI/PstI polymorphism were associated with decreased risk of liver cancer among east Asians (c2 vs. c1: OR = 0.75, 95%CI: 0.59-0.95, P = 0.016; c2/c2 + c2/c1 vs. c1/c1: OR = 0.76, 95%CI: 0.58-1.00, P = 0.050). In the stratified analysis by country, significant associations were observed between RsaI/PstI polymorphism and decreased risk of liver cancer among the Chinese population (c2 vs. c1: OR = 0.70, 95%CI: 0.54-0.91, P = 0.007; c2/c2 + c2/c1 vs. c1/c1: OR = 0.72, 95%CI: 0.54-0.95, P = 0.020), but not among Japanese and Korean populations. Results from the current meta-analysis indicates that the c2 allele of CYP2E1 RsaI/PstI (c1/c2) polymorphism may be a protective factor for HCC among east Asians, especially among China populations.

• 한국식품과학회지
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• 제19권4호
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• pp.377-381
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• 1987

수박씨기름의 triglyceride에서 산정(算定)가능한 분자종(分子種)은 26종류였으며 주요분자종(主要分子種)은 $C_{18:1}{\cdot}C_{18:2}{\cdot}C_{18:1}$ OLO 6.4%, $C_{18:0}{\cdot}C_{18:2}{\cdot}C_{18:2}$ SLL 7.1%, $C_{18:1}{\cdot}C_{18:2}{\cdot}C_{18:2}$ OLL 16.6%, $C_{16:0}{\cdot}C_{18:2}{\cdot}C_{18:2}$ PLL 19.6% 및 $C_{18:2}{\cdot}C_{18:2}{\cdot}C_{18:2}$ LLL 27.6%의 5종류였으며, 77.3%를 차지하였다.

• 한국식품영양과학회지
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• 제13권3호
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• pp.263-267
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• 1984

호도(胡桃)기름의 triglyceride 조성(組成)을 밝히기 위하여 TLC로서, 호도(胡桃)기름의 triglyceride를 분리하고, 이것을 HPLC에 의하여 PN별(別)로 triglyceride를 분획(分劃)하였다. PN 별(別)로 분획(分劃)한 각(各) 획분(劃分)을 다시 GLC에 의하여 acyl 탄소수별(炭素數別)로 분획(分劃)하였으며, 또한 PN별(別) 각획분(各劃分)의 지방산조성(脂肪酸組成)을 GLC로 분석(分析)하였다. 이들 세가지 chromatography의 분석결과(分析結果)로 부터 호도(胡桃)기름의 triglyceride 조성(組成)을 산정(算定)하였다. 산정(算定)한 호도(胡桃)기름의 triglyceride는 10종류였으며, 이들 중 1% 이상을 차지하는 triglyceride를 들면 다음과 같다. $(C_{18:2},\;C_{18:2},\;C_{18:2};\;53.3%)$, $(C_{18:1},\;C_{18:2},\;C_{18:2};\;10.1%)$, $(C_{18:1},\;C_{18:1},\;C_{18:2};\;5.4%)$, $(C_{18:1},\;C_{18:2},\;C_{18:3};\;4.3%)$, $(C_{18:0},\;C_{18:2},\;C_{18:2};\;3.9%)$, $(C_{18:0},\;C_{18:1},\;C_{18:2};\;2.0%)$, $(C_{18:0},\;C_{18:2},\;C_{18:2};\;1.8%)$.

• Applied Biological Chemistry
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• 제34권2호
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• pp.81-85
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• 1991

완두의 트리아실글리세롤의 조성을 구명하기 위하여 TLC로 트리아실글리세롤을 분리 한 후 HPLC를 이용하여 PN별로 분획하고 GLC로 분석 하였다. 이들 결과를 종합하여 트리아실글리세롤의 조성을 추정한 결과 건조된 완두의 트리아실글리세롤은 33종류였으며 주요 트리아실글리세롤은 $C_{16:0}C_{18:2}C_{18:2}(13.4%),\;C_{18:1}C_{18:2}C_{18:3}(9.3%),\;C_{18:1}C_{18:2}C_{18:2}(9.2%),\;C_{18:2}C_{18:2}C_{18:2}(8.1%),\;C_{18:2}C_{18:2}C_{18:3}(6.4%)$ 및 $C_{18:0}C_{18:1}C_{18:2}(5.4%)$이었다.

• Asian Pacific Journal of Cancer Prevention
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• 제15권13호
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• pp.5411-5416
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• 2014

Background: A number of studies have reported relationships of CYP2E1 RsaI/PstI polymorphisms with susceptibility to lung cancer in Chinese population. However, the epidemiologic results have been conflictive rather than conclusive. The purpose of this study was to address the associations of CYP2E1 RsaI/PstI polymorphisms with lung cancer risk in Chinese population comprehensively. Materials and Methods: Systematic searches were conducted in the PubMed, Science Direct, Elsevier, CNKI and Chinese Biomedical Literature Databases. Pooled odds ratios (ORs) with 95% confidence intervals (CIs) were calculated to estimate the strength of association. Results: Overall, we observed a decreased lung cancer risk among subjects carrying CYP2E1 RsaI/PstI c1/c2 and c1/c2+c2/c2 genotypes (OR=0.76, 95%CI: 0.64-0.90 and OR=0.78, 95%CI: 0.66-0.93, respectively), as compared with subjects carrying the c1/c1 genotype. In subgroup analysis, we observed a decreased lung cancer risk among c1/c2 carriers in hospital-based studies (OR=0.81, 95%CI: 0.68-0.98) and among carriers with c1/c2 and c1/c2+c2/c2 genotypes in population-based studies(OR=0.57, 95%CI: 0.42-0.79 and OR=0.58, 95%CI: 0.43-0.79, respectively), as compared with subjects carrying the c1/c1 genotype. Limiting the analysis to studies with controls in Hardy-Weinberg equilibrium (HWE), we similarly observed a decreased lung cancer risk among c1/c2 and c1/c2+c2/c2 carriers (OR=0.73, 95%CI: 0.60-0.88 and OR=0.73, 95%CI: 0.60-0.88, respectively), as compared with c1/c1. Conclusions: Our results suggested that CYP2E1 RsaI/PstI c1/c2 and c1/c2+c2/c2 variants might be a protective factor for developing lung cancer in Chinese population. Further well-designed studies with larger sample size are required to verify our findings.

• Journal of Plant Biology
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• 제11권1호
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• pp.33-37
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• 1968

Present investigation was carried out in order to make clear the fertility, morphological characters, and chromosome numbers of interspecific hybrids of Chrysanthemum Zauadskii, C. indicum, and C. lavandulaefolium. 1. Hybrids were not self-pollinated. When sib-crossed the fertility was 1.3~19.3%. 2. F2 individuals were variable in their morphological Characters. 3. Chromosome numbers of three putative parents were different from those reported previously: C. zawadskii 2n=36, C. indicum 2n=20, C. lavandulaefolium 2n=16, $C. indicum{\times}C. zawadskii 2n=28, C. zawadskii{\times}C. indicum-1 2n=28, C. zawadskii{\times}C. indicum-2 2n=28, C. zawadskii{\times}C. indicum-3 2n=28, C. zawadskii{\times}C. indicum-4 2n=28, C. zawadskii{\times}C. indicum-5 2n=28, C. zawadskii {\times}C. lavandulaefolium-1 2n=26, C. zawadskii{\times}C. lavandulaefolium-2 2n=26.$

• 한국식품과학회지
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• 제15권2호
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• pp.164-169
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• 1983

들깨기름의 트리-글리세리드조성(組成)을 밝히기 위하여 TLC에 의하여 시료유(試料油)로부터 트리-글리세리드를 분리하고, 이것을 ${\mu}-Bondapak\;C_{18}$ column을 사용한 HPLC에 걸어 PN별(別)로 트리-글리세리드를 분획(分劃)하였으며, 각획분(各劃分)을 분취(分取)하여 GLC로 아실 탄소수별(炭素數別)로 분획(分劃)하였다. 또한 PN별(別) 획분(劃分)의 지방산조성(脂肪酸組成)을 GLC에 의하여 분석하였다. 이들 분석 결과로부터 들깨기름을 구성하는 트리-글리세리드조성(組成)을 산정(算定)하였는데 그 수(數)는 15종류이며, 그 중 주요(主要)한 트리-글리세리드 및 그 비율은 다음과 같다. 즉, $(C_{18:3},\;C_{18:3},\;C_{18:3},\;68.0%)$, $(C_{18:2},\;C_{18:3},\;C_{18:3},\;6.7%)$, $(C_{18:1},\;C_{18:2},\;C_{18:3},\;5.9%)$, $(C_{16:0},\;C_{18:3},\;C_{18:3},\;4.3%)$, $(C_{18:1},\;C_{18:2},\;C_{18:3},\;3.8%)$, $(C_{18:1},\;C_{18:1},\;C_{18:3},\;3.2%)$, ($C_{16:0},\;C_{18:2},\;C_{18:3},\;2.0%)$, $(C_{18:2},\;C_{18:2},\;C_{18:3},\;1.5%)$ 및 $(C_{16:0},\;C_{18:1},\;C_{18:3},\;1.0%)$ 등이다.