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Identification of a SNP in Cattle HGD Gene with its Effect on Economic Trait in Hanwoo (한우 HGD 유전자내 변이지역과 경제형질간의 연관성 분석)

  • Han, Jung-Min;Kong, Hong Sik
    • Journal of Life Science
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    • v.24 no.11
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    • pp.1168-1173
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    • 2014
  • The homogentisate 1,2-dioxygenase (HGD) gene, which consists of 14 exons and spans approximately 42630bp on Bos taurus autosome 1 (BTA 1), is one of the six enzymes required for catabolism of the aromatic amino acids tyrosine and phenylalanine. It has been reported that BTA1 harbors quantitative trait loci that effect marbling score (MS), carcass weight (CW), and longissimus muscle area (LMA) in cattle. The aim of this study was to identify the single nucleotide polymorphisms (SNPs) in the HGD gene and to analyze their association with economic traits in Korean cattle (Hanwoo). Genetic polymorphisms were screened by direct sequencing, which detected 10 SNPs (T11187C, T11301A, T11398G, G29833A, G34256T, G34257C, T34284C, T42333G, T42348C, and T42468C). Six polymorphic sites were selected for genotyping, and economic traits were analyzed using a general linear model in Korean cattle (n=90). The observed genotype frequencies for G34256T were 0.5843(GG), 0.3708(GT), and 0.0449(TT). In addition, 0.3596(GG), 0.3708(GC), and 0.2697(CC) were observed for the G34257C mutation. Statistical association analysis revealed that G34256T polymorphisms were significantly associated with MS, and G34257C polymorphisms were significantly associated with MS and LMA (p<0.05). Further study is needed in order to use the genetic variant as a marker for marker-assisted selection in Korean cattle.

Dynamic Rheological Properties of Gelatin (젤라틴의 동적 레올로지 특성)

  • Choi, Yun-Hee;Lim, Seung-Taik;Yoo, Byoung-Seung
    • Korean Journal of Food Science and Technology
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    • v.34 no.5
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    • pp.830-834
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    • 2002
  • 본 연구는 동적 전단진동(dynamic shear oscillation) 측정방법을 이용하여 농도가 젤라틴의 동적 레올로지 특성에 미치는 영향에 대하여 관찰하였다. $5^{\circ}C$에서의 젤라틴의 저장 탄성률(storage modulus, G#)과 손실 탄성률(loss modulus, G@)은 농도가 증가함에 따라 증가하는 경향을 보였다. 0.9%를 제외한 모든 농도(2.0-4.9%)에서 젤라틴의 G#은 G@보다 매우 높은 값을 나타냈으며, G#과 G@이 ${\omega}$ 의존성이 없는 true 겔과 같은 거동을 나타냈다. 젤라틴의 농도와 K#(G#에 대한 절편)와의 관계에서는 높은 결정계수$(R^2=0.99)$를 보여주면서 좋은 상관관계를 나타냈다. 여러 온도범위$(-5,\;0,\;5,\;10^{\circ}C)$에서 각 온도별로 측정된 K#값은 $-5^{\circ}C(59.5Pa)\;>\;0^{\circ}C(4.09Pa)\;>\;5^{\circ}C(1.41Pa)\;>\;10^{\circ}C(0.35Pa)$의 순으로 가장 낮은 온도에서 K#값이 가장 높은 것으로 나타났다. 또한 냉각과정에서 최종냉각온도인 $5^{\circ}C$에서 측정된 젤라틴의 최대 G#수치는 4.9%(2399Pa)>4.1%(1744Pa)>3.0%(1159Pa)>2.0%(519.3Pa)>0.9%(3.15Pa)의 순으로 높은 농도에서 높게 나타났다. Aging 10시간 동안 젤라틴 겔의 구조형성은 겉보기 first-order kinetics로부터 겉보기 구조형성 속도상수(K)를 결정함으로써 파악될 수 있다. 젤라틴의 농도가 증가함에 따라 K값은 증가하였으며, 농도와 K값과의 관계는 높은 결정계수$(R^2)$를 나타내면서 좋은 상관관계를 보여주었다(Fig. 7). 10시간 aging 후 G#은주파수$({\omega})$ 의존성이 없이 독립적인 것으로 나타났다. 따라서 이들 젤라틴 겔은 aging하는 동안에 탄성이 점점 강해지고 10시간 후에는 가교결합의 밀도가 증가하여 강한 탄성을 가진 고무질 망상구조(rubber network)를 형성했음을 알 수 있다.

Heritabilities and Genetic Correlations on Economic Traits in Layers (난용계의 주요경제형질에 대한 유전력 및 유전상관)

  • 상병찬;한성욱;정선부
    • Korean Journal of Poultry Science
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    • v.16 no.2
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    • pp.73-81
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    • 1989
  • This study was conducted to estimate heritabilities, genetic and phenotypic correlations on economic traits in layers. The data analysis were the records of 351 pullets in S. C. W. Leghorn and 326 pullets in R. I. Red from March 1, 1980 to July 31.1981. The results obtained are summarized as follows:1 The average body weights at the first egg, 300 and 500 days of age were 1, 409, 1, 602 and 1, 709g S. C. W. Leghorn, and 1, 965, 2, 305, and 2, 479g in the R. I. Red, respectively. The age at first egg of the S. C. W, Leghorn and R. I. Red were 156 days and 163 days, respectively. The number of egg produced by the S. C. W. Leghorn and R. I. Red to 300 days and 500 days of age were 101.18, 214.39, and 101.05, 214.93, respectively. The egg weight at first egg, 300 and 500 days of age were 41.93, 57.65 and 60.33g in the S. C. W. Leghorn and 41.52, 57.62 and 60.57g in the R. I. Red, respectively. 2. The heritability estimates based on the variance of sire and dam components were 0.402-0.612 and 0.275-0.458 in the S. C. W. Leghorn, 0.309-0.523 and 0.134-0.380 in R. I. Red for body weight; 0.167 and 0.139 in the 5. C. W. Leghorn, 0.169 and 0.095 in the R.1. Red for age at first egg:0.214-0.139 and 0.336-0.341 in S. C. W Leghorn, 0.137-0.259 and 0.024-0.102 in the R. I. Red for number of egg production; 0.537-0.769 and 0.374-0.686 in the S. C. W. Leghorn, 0.519-0.631 and 0.116-0.365 in the R. I. Red for egg weight, respectively. 3. The genetic correlation coefficients of economic traits were as follows: In the S. C. W. Leghorn and R. I. Red , the coefficients between body weights and age at the first egg, 0.328-0.426 and 0.186-0.244:between body weights and number of egg production, -0.666--0.498 and -0.452--0.073:between body weight and egg weight, 0.384-0.774 and 0.126-0.612; between age at first egg and number of egg production, -0.639--0.452 and -0.754--0.320; between age at first egg and egg weight, 0.475-0.705 and 0.021-0.605; between number of egg production and egg weight, -0.623--0.355 and -0.861--0.327, respectively.

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Effect of Transglutaminase on the Rheological Properties of Fried Surimi Gel (Transglutaminase 첨가가 튀긴 어묵의 레올로지 특성에 미치는 영향)

  • Lee, Young-Seung;Jeong, Yoon-Hwa;Yoo, Byoung-Seung
    • Korean Journal of Food Science and Technology
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    • v.33 no.4
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    • pp.474-478
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    • 2001
  • Transglutaminase(TGase) 첨가가 튀긴 어묵의 레올로지 특성에 미치는 영향이 평가되었다. 최적의 TGase 함량과 setting시간은 저온$(25^{\circ}C)$ setting에서는 0.1%와 180분이었고, 고온$(40^{\circ}C)$ setting에서는 0.3%와 80분으로 각각 결정되었다. 각각의 최적조건에서의 겔 강도는 $25^{\circ}C$에서는 TGase를 첨가한 겔이 첨가하지 않은 겔보다 최대 750(g cm)도 증가했으며, $40^{\circ}C$에서는 약 800(g cm)정도의 증가를 보여주었다. 동적 점탄성의 setting 측정 결과로부터 TGase 첨가 시료는 $25^{\circ}C$인 경우 G#과 G@ 값이 각각 1.0 kPa과 0.5 kPa 정도 증가하는 것으로 나타났으며, $40^{\circ}C$ 경우는 G#과 G@ 값이 각각 15 kPa과 2.2 kPa 정도 증가함을 보여 주었다. 동적 점탄성의 가열-냉각 측정 결과에서도 $25^{\circ}C$$40^{\circ}C$ setting 모두에서 G#, G@ 값이 $5{\sim}15\;kPa$ 정도 증가함을 나타났다.

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Simultaneous Determination of Flavanone Glycosides in the Fruit of Citrus paradisi and C. grandis by HPLC-PDA

  • Piao, Xiang-Lan;Wu, Qian;Han, Saem;Kim, Hyun-Young;Lee, Sang-Hyun
    • Natural Product Sciences
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    • v.17 no.4
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    • pp.337-341
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    • 2011
  • An HPLC (high-performance liquid chromatography)-PDA (photodiode array) detection method was established for the determination of naringin, hesperidin and neohesperidin in the fruit of Citrus paradisi and C. grandis. The flavonoids were separated in less than 20 min using an YMC RP 18 column with isocratic elution using acetonitrile and water (23 : 77, v/v) at a flow rate of 1 ml/min, and a PDA detector. The levels of naringin, hesperidin and neohesperidin were 1345.92, 950.62, and 2078.82 ${\mu}g/g$, respectively, in the peel, and 102.43, 59.13, and 86.68 ${\mu}g/g$, respectively, in the flesh of C. paradisi. In C. grandis, the levels of naringin, hesperidin and neohesperidin were 3530.56, 80.00, and 5.26 ${\mu}g/g$, respectively, in the peel, and 59.59, 7.43, and 38.41 ${\mu}g/g$, respectively, in the flesh. The total content was highest in the peel, reaching 0.44% and 0.36% in C. paradisi and C. grandis, respectively, while the flesh contained only 0.025% and 0.011%, respectively. Therefore, the peels of C. paradisi and C. grandis are necessary for the processing and utilization of flavonoids.

Primary Production in the Osyter Farming Bay (굴 양식장 수역의 기초생산 연구)

  • LEE Byung-Don;KANG Hyung-Ku;KANG Yong-Joo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.24 no.1
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    • pp.39-51
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    • 1991
  • The primary production of phytoplankton in oyster farming bays were seasonally measured by $^{14}C$ method in Hansan-Koje Bay and Kamagyang Bay for one year period. In Hansan-Koje Bay, mean daily prima production was $0.52gC/m^2/day\;(0.22{\~}1.02gC/m^2/day)$, and it was relatively high in summer and low in winter. Annual primary production was estimated to be $189gC/m^2/yr.$. Mean chlorophyll a content was $1.70mg/m^3$, and it was relatively high in summer. In Kamagyang Bay, mean daily prima production was $0.91gC/m^2/day(0.11{\~}3.61gC/m^2/day)$. and it was maximum in summer and minimum in fall. Annual primary production was estimated to be $334gC/m^2/yr$. Mean chlorophyll a concentration was $2.34mg/m^3$, and it was maximum in summer and relatively high in winter. Considering the water area of the two osyter farming bays, annual carbon production by phytoplankton were 9,450 ton in Hansan-Koje Bay and 37,000 ton in Kamagyang Bay. In general pattern of primary production in these bays, high production occurs in summer with increasing trend since spring.

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Applications of Convolution Operators to some Classes of Close-to-convex Functions

  • Noor, Khalida Inayat
    • Honam Mathematical Journal
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    • v.10 no.1
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    • pp.23-30
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    • 1988
  • Let C[C, D] and $S^{*}[C,\;D]$ denote the classes of functions g, g(0)=1-g'(0)0=0, analytic in the unit disc E such that $\frac{(zg{\prime}(z)){\prime}}{g{\prime}(z)}$ and $\frac{zg{\prime}(z)}{g(z)}$ are subordinate to $\frac{1+Cz}{1+Dz{\prime}}$ $z{\in}E$, respectively. In this paper, the classes K[A,B;C,D] and $C^{*}[A,B;C,D]$, $-1{\leq}B<A{\leq}1$; $-1{\leq}D<C{\leq}1$, are defined. The functions in these classes are close-to-convex. Using the properties of convolution operators, we deal with some problems for our classes.

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A Study on the Silk Dyeing With Natural indigo Extracted from Polygoum tinctorium -On the fermentation dyeing- (쪽 色素에 의한 絹纖維 染色에 관한 硏究 -발효 염색에 대하여-)

  • Jeong, In-Mo;Nam, Seong-U
    • Journal of Sericultural and Entomological Science
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    • v.40 no.1
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    • pp.78-85
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    • 1998
  • Colorants were prepared by extraction of natural indigo which was harvested just in the blooming season(in the late of July). 100 g of fresh leaves soaking in 1 ιwater was kept at 3$0^{\circ}C$, 30 hours. A solution of 3g/l calcium hydroxide was added into it to precipitate dye substance and it was freezing-dried into powder form. The fermentation and dyeing conditions were investigated. The results obtained are summarized as follows; K/S value of dyed silk fabrics of fermentation conditions was higher at 95$^{\circ}C$ for 20 min. than at 4$0^{\circ}C$ for 20 hours. Furthermore, K/S value of dyed silk fabric was raised by the addition of 5g/l of glucose and 5g/l of NaOH. K/S value of dyed silk fabric was raised by the addition of 5g/l of glucose and 5g/l of NaOH. K/S value increased as extending of dyeing time when dyed till 2 hours at 3$0^{\circ}C$. K/S value decreased in order of 3$0^{\circ}C$, 4$0^{\circ}C$ and 5$0^{\circ}C$, at the various dyeing temperatures and dyeing concentrations, and colour fastness ranged from 4 to 5 grade in terms of washing, perspiration and light fastness.

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Mathematical Models of Respiration Rate of Cucumber Plants under Varying Environmental Conditions (환경 변화에 따른 오이의 호흡속도에 관한 수리적 모형)

  • 임준택;정현희;백선영;현규환;권병선;김학진;정순주;이범선
    • Journal of Bio-Environment Control
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    • v.9 no.4
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    • pp.185-192
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    • 2000
  • This study was conducted to develop mathematical model of respiration rate of cucumber plant under varying environmental conditions. 8.55% of gross photosynthesis of individual cucumber plant was used as respiration. Growth respiration coefficient was estimated as 0.0935. Maintenance respiration rate was estimated as 0.00158g CH$_2$g$^{-1}$ .h$^{-1}$ at 24$^{\circ}C$ of air temperature and it increased exponentially as air temperature became higher. Respiration rate decreased poportionally as content of storage carbohydrate became lower. Ion uptake respiration rate of roots was estimated as 0.6648g C$H_2O$.(gN)$^{-1}$ .

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Fermentative Characteristics of Extruded Meju by the Molding Temperature (메주의 압출성형에서 성형온도에 따른 메주의 발효특성)

  • 변명우;김동호;육홍선;김기연;신명곤
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.30 no.2
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    • pp.250-255
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    • 2001
  • Effect of molding temperature on the quality changes of extruded meju was studied. Meju was molded at 8$0^{\circ}C$, 6$0^{\circ}C$ and 4$0^{\circ}C$, and then stored at $25^{\circ}C$ with 50% of relative humidity for 30 days. The texture of the cooked soybean grain showed that the firmness and cohesiveness were increased, and consistency was decreased by decrease of molding temperature. The density of the meju molded at 8$0^{\circ}C$, 6$0^{\circ}C$ and 4$0^{\circ}C$ were 1.072g/mL, 1.079g/mL and 1.203g/mL, respectively. The meju molded at 4$0^{\circ}C$ had significantly higher density than those molded at 8$0^{\circ}C$ or 6$0^{\circ}C$. Also, delay of water evaporation, acidification, and rapid growth of fungal mycellium were observed on the sample with molding temperature at 4$0^{\circ}C$ during fermentation. Activity of amylase and protease, contents of total reducing sugar and amino nitrogen of 4$0^{\circ}C$-molded meju were represented lower level than those of 6$0^{\circ}C$ or 8$0^{\circ}C$-molded sample. Therefore, it was considered that the molding temperature was an important factor for meju fermentation and molding temperature of 6$0^{\circ}C$ or over would be acceptable.

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