• 종양간호연구
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• 제8권1호
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• pp.1-7
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• 2008

Purpose: Peripheral blood stem cell transplantation (PBSCT) has been widely used. The optimal time for collection is a critical factor to obtain proper counts of CD34 cell by peripheral blood stem cell collection (PBSC). The purpose of this study was to identify the factors influencing peripheral blood stem cell collection in order to figure out the more effective timing for PBSC. Method: The subjects of this study were 189 patients undergoing 3 leukapheresis from January 28, 2005 to December 31,2006. Group's characteristics, checkup opinion of pre-peripheral blood on the day of harvest & outcome of PBSC were analyzed and evaluated using SAS statistics program after grouping patients as below; group 1-CD34 cell counts $<2{\times}10^6/kg$ (n=97); group $2-2{\times}10^6/kg$ ${\leq}CD34$ cell counts $<4{\times}10^6/kg$ (n=26); group 3-CD34 cell counts ${\geq}4{\times}10^6/kg$ (n=63). Results: Based on outcome of peripheral blood stem cell according to diagnosis, acute myelocytic leukemia (AML) was 65.5% at Group 1, Lymphoma was 21.7% at Group 2 and multiple myeloma (MM) was 70.8% at Group 3. There were significant differences in CD34 cell counts according to diagnosis (p=0.00004). Type of cytokine mobilization according to diagnosis, Lenograsim was using 62.5% of MM & 38.2% of AML and filgrastim is using 22.0% of AML only. Circular peripheral blood CD34 cell counts prior to harvest was $258.1/{\mu}L$ at Group 3 which was much higher comparing to Group 1 ($10.5/{\mu}L$) and Group 2 ($39.9/{\mu}L$) (p<0.001). TNC counts of collected peripheral blood stem cell was $15.36{\times}10^6/kg$ at Group 3 and it's much higher than Group 2 ($13.16{\times}10^6/kg$) and Group 1 ($12.36{\times}10^6/kg$) (p=0.083). There was no significant difference in MNC counts inbetween 3 groups. Conclusions: Circular peripheral blood CD34+ cell counts prior to harvest was much higher at Group 3 than Group 1 and Group 2. Therefore, the number of CD34+ cells on the day of harvest can be used as an accurate predictor for peripheral blood stem cell.

• Journal of Gastric Cancer
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• 제22권4호
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• pp.348-368
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• 2022

Purpose: Chromosomal instability is a hallmark of gastric cancer (GC). It can be driven by single nucleotide variants (SNVs) in cell cycle genes. We investigated the associations between SNVs in candidate genes, PLK2, PLK3, and ATM, and GC risk and clinicopathological features. Materials and Methods: The genotyping study included 542 patients with GC and healthy controls. Generalized linear models were used for the risk and clinicopathological association analyses. Survival analysis was performed using the Kaplan-Meier method. The binding of candidate miRs was analyzed using a luciferase reporter assay. Results: The PLK2 C_rs15009-C_rs963615 haplotype was under-represented in the GC group compared to that in the control group (P_corr=0.050). Male patients with the PLK2 rs963615 CT genotype had a lower risk of GC, whereas female patients had a higher risk (P=0.023; P=0.026). The PLK2 rs963615 CT genotype was associated with the absence of vascular invasion (P=0.012). The PLK3 rs12404160 AA genotype was associated with a higher risk of GC in the male population (P=0.015). The ATM T_rs228589-A_rs189037-G_rs4585 haplotype was associated with a higher risk of GC (P<0.001). The ATM rs228589, rs189037, and rs4585 genotypes TA+AA, AG+GG, and TG+GG were associated with the absence of perineural invasion (P=0.034). In vitro analysis showed that the cancer-associated miR-23b-5p mimic specifically bound to the PLK2 rs15009 G allele (P=0.0097). Moreover, low miR-23b expression predicted longer 10-year survival (P=0.0066) in patients with GC. Conclusions: PLK2, PLK3, and ATM SNVs could potentially be helpful for the prediction of GC risk and clinicopathological features. PLK2 rs15009 affects the binding of miR-23b-5p. MiR-23b-5p expression status could serve as a prognostic marker for survival in patients with GC.

• 한국멀티미디어학회논문지
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• 제21권1호
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• pp.34-44
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• 2018

An early detection of intravenous (IV) infiltration is essential to minimize the injuries during infusion therapy, which is one of the most important tasks for nurses in nursing settings. We report that bioelectrical impedance analysis is useful in the early detection of infiltration at puncture sites. When infiltration was intentionally induced in the vein of a pig's posterior ear, impedance parameters (R, $X_C$, $C_m$) showed significant differences before and after infiltration. In particular, the relative resistance ($R/R_{BI}$) decreased significantly at infiltration and then slowly decreased. This indicates that the vein in pig's ear is thin and the amount of surrounding subcutaneous tissue, and hence the infiltrated solution accumulates slowly after infiltration. However, when infiltration was induced in the vein of human's forearm, the relative resistance at 20 kHz decreased gradually over time. In the $R-X_C$ graph, the positions in the case of infiltration induced in the pig' ear shifted rapidly before and after infiltration, whereas the positions in the case of infiltration induced in the human's forearm moved gradually during infiltration. Our findings suggest that the impedance parameters (R, $R/R_{BI}$, $X_C$, R vs. $X_C$, and $C_m$) are effective indicators to detect the infiltration early in a non-invasive and quantitative manners.

• 자원리싸이클링
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• 제20권5호
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• pp.34-39
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• 2011

금속급 실리콘(MG-Si)에 Ca을 첨가하여 Ar 분위기 중의 1500$^{\circ}C$ 에서 용융하고, 10$^{\circ}C$/min의 속도로 상온까지 응고하여 왕수로 침출하였다. 본 연구에서는 금속급 실리콘 중의 Fe와 P의 제거에 미치는 Ca 첨가의 영향과 산 침출 조건의 영향에 대하여 조사하였다. Ca 첨가에 의해 MG-Si의 결정립계에 CaSi$_2$상이 생성되고, CaSi$_2$상 내에 FeSi$_2$상이 석출된 것을 확인하였다. 이러한 CaSi$_2$상 등의 생성에 의해 600~850${\mu}m$ 크기의 MG-Si에서도 30% 이상의 왕수를 이용한 침출로 Fe의 97%, P의 66%까지 제거할 수 있었다.

• 전기화학회지
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• 제12권1호
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• pp.34-39
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• 2009

니들 코크스의 활성화를 위해 $HNO_3$와 $NaClO_3$ 혼합용액에서의 산처리와 열처리를 행하였다. 산화처리 코크스와 열처리한 코크스의 미세구조는 XRD, FESEM, elemental analysis, BET, Raman spectroscopy를 이용하였으며, 전기이중층 거동은 충방전 분석을 행하였다. 니들 코크스는 산화처리 시간에 따라 산소의 중량 %의 증가와 함께 층간이 분리되어(001) 구조로 상변화가 일어나고, $200^{\circ}C$ 이상의 열처리에서 흑연구조 특성인(002) 구조로 되돌아갔다. 이들 산화처리 과정에서 층간에 관능기가 도입 되어 구조결함이 발생하고 1차 충전에서 전계 활성화에 의해 층간을 확장되어 2차 충전에서 전기이중층 용량을 발생 시키는 것으로 보인다. 24시간 산화처리후 $300^{\circ}C$ 열처리한 코크스의 2.5 V까지의 2 전극 기준에서 구한 활물질 중량 당 용량과 전극 부피 당 용량은 각각 32.1 F/g과 29.5 F/ml을 나타내었다.

• 한국연소학회지
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• 제19권3호
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• pp.34-43
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• 2014

The reformer system is a method for hydrogen production from hydrocarbon fuels such as natural gas under high temperature environment($about{\sim}1,000^{\circ}C$). The premixed swirl burner with mixing swirler and combustion swirler designed to deliver fuel cell electric output from 0.5 kW to 1.5 kW. Premixed swirl burner experiments using natural gas and mixture of natural gas and anode off gas were carried out to analyse flame patterns and stability by equivalence ratio respectively. The results show that the stable swirl flame can be established for all cases of fuels type using the premixed swirl burner. The swirl flame had a wide stability region and it showed very low CO(50 ppm) and $NO_x$(20 ppm) emission at different fuel type and various equivalence ratio conditions. The operating range of premixed swirl burner for stable swirl flame is found to exist between equivalence ratio of 0.70 to 0.90 for turn down ratio of 3:1.

• BMB Reports
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• 제54권10호
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• pp.522-527
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• 2021

Lysine-specific demethylase 1 (LSD1) is an epigenetic regulator that modulates the chromatin status, contributing to gene activation or repression. The post-translational modification of LSD1 is critical for the regulation of many of its biological processes. Phosphorylation of serine 112 of LSD1 by protein kinase C alpha (PKCα) is crucial for regulating inflammation, but its physiological significance is not fully understood. This study aimed to investigate the role of Lsd1-S112A, a phosphorylation defective mutant, in the cigarette smoke extract/LPS-induced chronic obstructive pulmonary disease (COPD) model using Lsd1^SA/SA mice and to explore the potential mechanism underpinning the development of COPD. We found that Lsd1^SA/SA mice exhibited increased susceptibility to CSE/LPS-induced COPD, including high inflammatory cell influx into the bronchoalveolar lavage fluid and airspace enlargement. Additionally, the high gene expression associated with the inflammatory response and oxidative stress was observed in cells and mice containing Lsd1-S112A. Similar results were obtained from the mouse embryonic fibroblasts exposed to a PKCα inhibitor, Go6976. Thus, the lack of LSD1 phosphorylation exacerbates CSE/LPS-induced COPD by elevating inflammation and oxidative stress.

• 미생물학회지
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• 제48권3호
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• pp.207-211
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• 2012

이 연구의 목적은 코리네형 균주(Corynebacterium glutamicum)에 존재하는 acyltransferase 유전인자의 발현에 관여하는 autoinducer에 대한 정보를 얻기 위해 다양한 종류의 균주에서 얻은 세포배양액(cell-free culture fluids)을 처리하여 발현에 미치는 효과를 조사하는 것이다. 다양한 배양시간동안 얻은 Agrobacterium tumefaciens의 세포배양액은 acyltransferase의 발현을 전혀 증가시키지 못하는 것으로 관찰되었다. 반면 AI-1과 AI-2를 분비하는 것으로 알려진 Vibrio harveyi BB120($AI-1^+$, $AI-2^+$)을 지수성장기까지 배양시켜 얻은 세포배양액은 acyltransferase의 발현을 증가시키는 것으로 관찰되었다. 이들 autoinducer가 미치는 효과를 조사하기 위하여 돌연변이 균주인 MM77 ($AI-1^-$, $AI-2^-$)과 BB152 ($AI-1^-$, $AI-2^+$)를 사용하여 조사한 결과, 이들 균주에서 얻은 세포배양액은 BB120와는 달리 acyltransferase의 발현을 증가시키지 않는 것으로 나타났다. 이러한 결과로 보아 acyltransferase의 발현은 AI-1에 의한 효과로 보이며, 이를 확인하기 위해 V. harveyi BB152와 같이 AI-2만을 분비하는 것으로 알려진 Escherichia coli ($AI-1^-$, $AI-2^+$)를 사용하였다. 하지만 E. coli 세포배양액은 acyltransferase의 발현을 증가시키는 것으로 관찰되었다. 이들 결과는 코리네형 균주에 존재하는 autoinducer는 기존에 알려진 형태의 신호분자와 차이가 있을 것이며, 코리네형 균주외에 V. harveyi와 E. coli에도 존재하여 종간 상호작용(interspecies communication)에 관여할 것으로 보인다.

• IMMUNE NETWORK
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• 제21권6호
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• pp.43.1-43.14
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• 2021

Group 3 innate lymphoid cells (ILC3), which express IL-22 and IL-17A, has been introduced as one of pathologic cells in axial spondyloarthritis (axSpA). Dyslipidaemia should be managed in axSpA patients to reduce cardiovascular disease, and dyslipidaemia promotes inflammation. This study aimed to reveal the role of circulating ILC3 in axSpA and the impact of dyslipidaemia on axSpA pathogenesis. AxSpA patients with or without dyslipidaemia and healthy control were recruited. Peripheral blood samples were collected, and flow cytometry analysis of circulating ILC3 and CD4⁺ T cells was performed. The correlation between Ankylosing Spondylitis Disease Activity Score (ASDAS)-C-reactive protein (CRP) and circulating immune cells was evaluated. The effect of oxidized low-density lipoprotein cholesterol (oxLDL-C) on immune cell differentiation was confirmed. AxSpA human monocytes were cultured with with oxLDL-C, IL-22, or oxLDL-C plus IL-22 to evaluate osteoclastogenesis using tartrate-resistant acid phosphatase (TRAP) staining and real-time quantitative PCR of osteoclast-related gene expression. Total of 34 axSpA patients (13 with dyslipidaemia and 21 without) were included in the analysis. Circulating IL-22⁺ ILC3 and Th17 were significantly elevated in axSpA patients with dyslipidaemia (p=0.001 and p=0.034, respectively), and circulating IL-22⁺ ILC3 significantly correlated with ASDAS-CRP (Rho=0.4198 and p=0.0367). Stimulation with oxLDL-C significantly increased IL-22⁺ ILC3, NKp44^- ILC3, and Th17 cells, and these were reversed by CD36 blocking agent. IL-22 and oxLDL-C increased TRAP⁺ cells and osteoclast-related gene expression. This study suggested potential role of circulating IL-22⁺ ILC3 as biomarker in axSpA. Furthermore, dyslipidaemia augmented IL-22⁺ ILC3 differentiation, and oxLDL-C and IL-22 markedly increased osteoclastogenesis of axSpA.

• 대한수의학회지
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• 제5권1호
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• pp.61-96
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• 1965

Observations were made on the blood picture of total 196 heads of healthy Korean cattles, including 98 males and 98 females in the purpose of determination of hematological values and its sex difference, and seasonal variations during one year period from December 1963 to November 1964. The blood sampling were scheduled by random in four different seasons and the sample size of both sex included in each season were designated to be same size. The ranges, averages or mean values of the erythrocytes, hemoglobin, hematocrit, mean corpuscular hemoglobin concentration, total white blood cell count and differential count were determined in this studies and their respective standard deviation, standard error of means, sex defferences and seasonal variations were as follows; 1. The erythrocyte count of male showed a range of $5.0{\times}10^6/c.mm$ to $8.75{\times}10^6/c.mm$ with a mean of $6.5{\pm}0.096{\times}10^6/c.mm$. Female showed a range of $5.0{\times}10^6/c.mm$ to $8.30{\times}10^6/c.mm$, with a mean of 6. $131{\pm}0.078{\times}10^6/c.mm$. There was a highly significant sex difference and seasomal variation was not found to be significant. 2. The hemoglobin value of male showed a range of 9.0g/100cc. to 14.5g/100cc. with a mean of $11.074{\pm}0.143g/100cc$. Female showed a range of 9.0g/100cc to 13.0g/100cc. with a mean of $10.745{\pm}0.034g/100cc$. There was a highly significant sex difference and seasonal variation was not found to be significant. 3. The hematocrit value of male showed a range of 28% to 45% and with a mean of $34.867{\pm}0.468%$. Female showed a range of 28% to 42% with a mean of $32.888{\pm}0.322%$. There was a highly significant sex difference and seasonal variation was not found to be significant. 4. The mean corpuscular hemoglobin of male showed a range of 14.4rr. to 19.6rr. with a mean of $17.1{\pm}0.112rr$. Female showed a range of 14.7rr. to 19.5rr. with a mean of $17.6{\pm}0.113rr$. 5. The mean corpusular volume of male showed a range of $42.5{\mu}^3$ to $62.2{\mu}^3$ with a mean of $53.9{\pm}0.419{\mu}^3$, Female showed a range of $44.2{\mu}^3$ to $60.0{\mu}^3$ with a mean of $53.8{\pm}0.375{\mu}^3$. 6. The mean corpuscular hemoglobin concentration of male showed a range of 28.1 % to 34.9% with a mean of $31.4{\pm}0.161%$. Female showed a range of 28.0% to 34.9% with a mean of $30.9{\pm}0.169%$. 7. The total leucocyte count of male showed a range of 4,000/c.mm to 13,100/c.mm. with a mean of $9,338{\pm}218.23/c.mm$. Female showed a range of 4,000/c.mm. to 14,000/c.mm. with a mean of $9,338{\pm}235.90/c.mm$. Six difference was not found to be significant and there was a highly significant seasonal variation. 8. The differential count of male, the means of neutrophil, stab, segmented cell, Iymphocyte, monocyte, eosinophil and basophil were $31.173{\pm}0.570%$, 0.3%. $30.867{\pm}0.564%$, $55.112{\pm}0.603%$, $3.745{\pm}0.082%$, $9.867{\pm}0.422%$ and 0.14% rspectively. Female showed means of $31.010{\pm}0.572%$, 0.2%, $30.806{\pm}0.569%$, $53.929{\pm}0.634%$, $4.082{\pm}0.109%$, $10.908{\pm}0.503%$ and 0.12% respectively. There were significant sex differences in monocyte and highly significant sex difference in eosinophil, and seasonal variation were found to be highly significant in neutrophil, monocyte and eosinophil. 9. Hematological comparison made between cattles infested with so called "small type piroplasma" and non-infested group. The result of investigation showed no significant difference upon the red blood cell, hemoglobin and hematocrit values between lighty infested group and non-infested group. 10. Age distribution of test group in this study ranged from 2 years to 6 years in both sex and their average age were $4.45{\pm}0.114$(male) and $4.50{\pm}0.116$(female). There found to be no significant sex difference and seasonal variations in the age of test group.