• Journal of Life Science
• /
• v.25 no.8
• /
• pp.925-931
• /
• 2015

We performed a molecular marker-based analysis of quantitative trait loci (QTLs) for traits that determine the quality of the appearance of grains, using 120 doubled-haploid (DH) lines developed by another culture from the F1 cross between ‘Cheongcheong’ (Oryza sativa L. ssp. Indica) and ‘Nagdong’ (Oryza sativa L. ssp. Japonica). The traits studied included length, width, and thickness of the grains, as well as length-to-width ratio and 1,000-grain weight. The objective of this study was to determine the genetic control of these traits in order to formulate a strategy for improving the appearance of this hybrid. Within the DH population, five traits exhibited wide variation, with mean values occurring within the range of the two parents. Three QTLs were identified for grain length on chromosomes 2, 5, and 7. Three QTLs were mapped for grain width on chromosome 2: qGW2-1, qGW2-2, and qGW2-3. Six chromosomes were identified for the grain length-to-width ratio; four of these were on chromosome 2, whereas the other two were on chromosomes 7 and 12. One QTL influencing 1,000-grain weight was identified and located on chromosome 8. The results presented in the present study should facilitate rice-breeding, especially for improved hybrid-rice quality.

• The Korean Journal of Mycology
• /
• v.43 no.3
• /
• pp.200-205
• /
• 2015

Perilla frutescens var. japonica Hara was collected from farmland in Seongju-gun. Fifteen endophytic fungal strains with different colony morphologies were isolated from roots of P. frutescens. Waito-c rice seedlings were treated with the concentrated culture filtrates (CF) of endophytic fungi for observation of their plant growth-promoting activities. In the results, the CF of Y2H001 fungal strain promoted the growth of the waito-c rice seedlings. The phylogenetic tree of Y2H001 strain was analyzed by the combined sequences of the partial internal transcribed spacer region (ITS) and partial betatubulin gene. Molecular and morphological studies identified the Y2H001 strain as belonging to Aspergillus flavus. In gas chromatography mass spectrometry (GC/MS) analysis of the CF of Y2H001 strain, gibberellic acid (GA) was detected and quantified. Therefore, we describe Y2H001 strain as a new $GA_3$-producing A. flavus based on morphological, molecular characteristics and analysis of secondary metabolite.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.30 no.2
• /
• pp.225-238
• /
• 1997

Zooplankton distribution in the front zone was investigated in the East Sea of Korea (Sea of Japan). More than 100 taxa appeared in the study area, which was far diverse appearance being compared with those in West (Yellow Sea) and South Sea of Korea. In Nov. 1994, Paracalanus parvus, Oikopleura spp., and Noctiluca scintillans, which preferred warmer environment, predominated at the collection sites in the front zone, especially at warmer surface layer. But in Nov. 1995, when the surface water temperatures were about $2^{\circ}C$ lower than those of the previous year, cold water species of copepod Metridia lucens, immature forms of Calanus and Sagitta, and crustacean eggs dominated in the collection sites in front zone, which were geographically not Identical with those of the previous year. Vertical distributional patterns were not coincide among the taxa. This suggests that zooplankton has different specific habitat characteristics with temperatures. In general, those taxa with preference of warmer environments showed high probability of co-occurrance with low abundances while those that preferring colder environments showed low probability of co-occurrance with high abundances. It seems that warm water contributes to the high diversity of zooplankton in the front zone while cold water does to the high biomass.

• Korean Journal of Plant Resources
• /
• v.29 no.2
• /
• pp.189-203
• /
• 2016

This study was carried out to investigate the flora of Jingangsan Mt. (Ganghwa-gun). The vascular plants identified during the 11 round field surveys were a to total of 560 taxa: 114 families, 336 genera, 495 species, 7 subspecies, 53 varieties, 4 forms and 1 hybrid. A high plant diversity were Poaceae (11.0%), Asteraceae (10.8%), Cyperaceae (8.8%), Rosaceae (4.6%) and Lamiaceae (4.3%) in regular order. The four taxa of Korean endemic plants such as Viola seoulensis Nakai, Salix koriyanagi Kimura ex Goerz, Hemerocallis hakuunensis Nakai and Polygonatum infundiflorum Y. S. Kim, B. U. Oh & C. G. Jang were collected. The vascular plants on the red list according to IUCN evaluation basis were found to be four taxa: Near Threatened (NT) species of Delphinium maackianum Regel, and Not Evaluate (NE) species of Mosla japonica (Benth. ex Oliv.) Maxim., Carex paxii Kük. and Polygonatum infundiflorum Y. S. Kim, B. U. Oh & C. G. Jang. The floristic regional indicator plants found in this area were 28 taxa comprising two taxa of degree IV, three taxa of degree III, eight taxa of degree II, and 15 taxa of degree I. In addition, the naturalized plants were identified as 44 taxa and the percentage of naturalized index (NI) was 7.9%, and Urbanization Index (UI) was 13.7%.

• Journal of Life Science
• /
• v.20 no.9
• /
• pp.1339-1344
• /
• 2010

In order to produce environmental-friendly fermented compost, a cattle manure-sawdust compost (antifungal compost) was developed by inoculation of B. licheniformis KJ-9 to cattle manure-sawdust. The thermal stability of the antifungal substance produced by B. licheniformis KJ-9 maintained more than 60% antifungal activity with heat treatment at $100^{\circ}C$ for 10 min, and the optimum pH of antifungal activity of the substance was 7.0. In a pot experiment with red pepper, the antifungal compost increased 1.5~2 times in leaf number and stem and root growth rate compared to those of commercial compost. Also, the diameter of stems increased 1.5-3 times in the antifungal compost treated group. The amount of microbes increased markedly in soil supplemented with antifungal compost compared to the control. In the field experiment for cultivation of garlic and Perilla japonica, the growth of both crops was significantly enhanced in the field treated with antifungal compost as compared to the commercial compost.

• Journal of Life Science
• /
• v.27 no.6
• /
• pp.611-616
• /
• 2017

The antibacterial activities of nine oriental herbal medicine extracts were measured against seven kinds of bacteria known to cause food poisoning and disease. The extracts were prepared with distilled water using an autoclave ($121^{\circ}C$ for 30 min). The extraction yields of nine oriental herbal medicines were in the rage of 0.5%-33.4%. The antibacterial activities were evaluated using the paper-disc method. Extracts of Hwangryeon, Jiyu, and Ohbaeja showed antibacterial activities while the others did not. Extracts of Coptis japonica, Hwangryeon, and Sanguisorba officinalis, Jiyu showed antibacterial activities only against Clostridium perfringens and Listeria monocytogenes. Extracts of Rhus javanica, Ohbaeja showed the strongest antibacterial activities against all the bacteria tested. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of R. javanica extracts were in the range of 2.4~20 mg/ml for the bacteria tested. In conclusion, the extract of R. javanica showed superior antibacterial activities against the seven bacterial strains tested. The MIC and MBC values for each bacterium were represented as strain names (MIC, MBC), which were Bacillus cereus (11.2, 20), Clostridium perfringens (2.4, 4.7), Escherichia coli (9.1, 9.1), Listeria monocytogenes (11.1, 20), Salmonella typhi (4.7, 9.1), Staphylococcus aureus (2.4, 4.7), and Vibrio parahaemolyticus (2.4, 5.3). Further purification and study of this antibacterial material would be helpful for developing antibiotics or promoting synergistic effects with known antibiotics, producing antibacterial material for lengthening the shelf-life of food, and so on.

• Korean Journal of Poultry Science
• /
• v.35 no.1
• /
• pp.29-37
• /
• 2008

This study was evaluated the oxidative stability of the breast and leg meat from chicken fed extract of medicinal herb mix (Lonicera japonica Thunberg : Morus alba L. : Coptis chinensis = 48.5 : 48.5 : 3.0%) and antibiotics (Albac G150). Ross chickens were divided into three treatments: basal diet (control), basal diet with antibiotics (0.05%) and basal diet with extract of medicinal herb mix (0.3%). They were fed the experimental diets for 35 days and slaughtered. After that, the breast and leg meat samples were stored at $4^{\circ}C$ for 10 days. Total phenol content of breast meat in Ross chicken fed extract of medicinal herb mix was higher than that of antibiotics treatment and control during storage. DPPH radical scavenging activity was the highest in leg meat of Ross chicken fed extract of medicinal herb mix at 0 day. $ABTS^+$ reduction activity was higher in chicken meat fed extract of medicinal herb mix than antibiotics treatment and control. This results showed that the diet with extract of mixed medicinal herb was more effective in oxidative stability of chicken meat than basal diet and diet with antibiotics.

• Journal of Life Science
• /
• v.14 no.5
• /
• pp.829-833
• /
• 2004

The major compositions of Lentinus edodes seasoning(LES) compose of 30.3% of Lentinus edodes fruit body, 18.1 % of anchovy (Engraulis japonicus) powder and 51.6% of sea tangle (Laminaria japonica), prawn and green tea powder and so on were investigated in order to evaluate the nutritional value. Sodium concentration in LES was high up to 5,042.99 mg/IOO g and K, Ca, P, Mg, Fe, Zn, Mn and Cu were followed. There were nineteen total amino acids in LES. The glutamic acid content was high up to 4.62 mg/100 mg and glycine, leucine, alanine, aspartic acid were followed. Among twenty free amino acids, glycine content was high up to 2.37 mg/100 mg and glutamic acid, asparanine, proline, taurin and aspartic acid were followed. The contents of vitamin C in LES was high up to 224.78 mg/100 g and pantothenic acid, vitamin B$_{6}$, niacin, vitamin E, folic acid, vitamin B$_{l}$ vitamin B$_{2}$, vitamin D$_{3}$, vitamin A, vitamin B$_{12}$ and vitamin $K_{l}$ were followed.wed.

• Asian Journal of Turfgrass Science
• /
• v.16 no.2
• /
• pp.65-73
• /
• 2002

Studies were conducted to determine the effect of undersoil heating on growth and quality of turfgrasses including Kentucky bluegrass (Poa pratensis L.‘Nuglade’), perennial ryegrass (Lolium perenne L.‘Accent’), tall fescue (Festuca arundinacea Schreb.‘Pixie’), and Korean lawngrass (Zoysia japonica Steud.) in simulated athletic field during winter season in Korea. Mineral contents in clippings of turfgrasses grown at different soil mixtures and temperatures were also analyzed. Undersoil heating (approximately 20$\pm$2$^{\circ}C$) was effective in protecting turfgrasses except Korean lawngrass from freezing injury and discoloration of shoots due to extremely cold temperatures during midwinter. Among turfgrasses grown at undersoil heating zone, tall fescue and perennial ryegrass showed the highest clipping weights and chlorophyll contents, respectively. However, anthocyanin contents of shoots were higher in Kentucky bluegrass. There was little or no difference in clipping weights, chlorophyll contents, anthocyanin contents and greenness of shoots between turfgrasses grown at two soil mixtures composed of 80% sand＋10% peat moss＋10% soil (v/v/v) and 80% sand＋20% pea moss (v/v). Contents of mineral K, Ca and Mg in clippings of cool-season turfgrasses were comparatively higher in a soil mixture composed of 80% sand＋10% peat moss＋10% soil, but little difference in contents of N and P was observed between two soil treatments. Results indicated that undersoil heating can improve quality of turf surface by thawing soil, melting snow, and maintaining shoot growth and greenness of turfgrasses in sports field during winter season.

• Korean Journal of Ichthyology
• /
• v.29 no.4
• /
• pp.235-243
• /
• 2017

Reproductive characteristics of Rhodeus pseudosericeus (Pisces: Acheilognathinae) was investigated at the Heukcheon (River), Yangpyeong-gun, Gyeonggi-do, Korea from January to July in 2012. This fish prefers heavily vegetated stagnant pool with Phragmites japonica and Salix koreensis and area with high proportions of sand and mud. The standard length of the population indicated that 13~22 mm group is 0-year old, 30~45 mm group is 1-year old, 45~55 mm group is 2-year old and 45~70 mm group is more than 3-year old (June in 2012). The sex ratio of female to male was 1 : 0.80. The spawning season was from March to June with water temperatures in the range $11.0{\sim}26.2^{\circ}C$. The embryos were found with no egg mass in Unio douglasiae sinuolatus and the number of those was 1~22 ($5.8{\pm}5.51$). The number of the embryos found in each of the four parts of the mussel gill was 1~9 ($2.7{\pm}2.16$) in left outer, 1~2 ($1.5{\pm}0.71$) in left outer, 1~5 ($1.8{\pm}1.79$) in left outer and 1~22 ($5.8{\pm}5.51$) in left outer.