• Journal of Animal Science and Technology
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• v.46 no.4
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• pp.667-676
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• 2004

Yogurt was prepared from skim milk added with 1, 2, 4 or 6% of rice- or skim milk powders and commercial lactic acid bacterial starters. Changes in pH, titratable acidity, viable cell counts, viscosity, organic acid contents and carbohydrates during fermentation were monitored and its sensory evaluation was also performed. The optimum level of additives such as rice- and skim milk powders for yogurt manufacture was selected. Fermentation properties of yogurt added with rice and skim milk powders were studied. The control yogurt reached pH 4.5 after 10 hours of fermentation, whereas the samples added with 4 or 6% rice powders reached pH 4.5 in 6 hours and those added with skim milk powder reached in 8 hours. After 4 hours of fermentation, the control yogurt reached a titratable acidity at 1.0 %, whereas other samples exceeded 1.0%. After 4 hours of fermentation, the viable cell counts in the samples added with 4% rice powders were the highest. At the level of 4% rice powders, the curd viscosity decreased. Except for the sample added with 6% skim milk powders, all the samples produced higher lactic acid during fermentation, and galactose level in yogurt was the highest when added with 4% rice powder or 6% skim milk powder. In overall sensory evaluation using 5 parameters, the sensory scores of all the yogurts were not significantly different. However, the sample added with 4% skim milk powders was the best for color and overall acceptability. The sample added with 4% rice powders was the best for the flavor and texture. From this experiment, the optimun level of additives such as rice or skim milk powders was selected to be 4%.

• Journal of Animal Science and Technology
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• v.46 no.4
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• pp.571-584
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• 2004

It is known widely that granulosa cell apoptosis leads follicular atresia and macrophages exert their effects directly and/or indirectly from the initiation to the completion of follicular atresia by phagocytosis of apoptotic bodies and secretion of various cytokines. However, the site of initiation, propagation routes and the elimination methods of apoptotic bodies, and the time and methods of penetration of macrophages into the follicles are not known completely. Using pig(Yorkshire-breed) ovary, immunohistochemical studies with TUNEL for apoptotic bodies and pig macrophage monoclonal antibody 4E9 for macrophages, and light and transmission electron microscopic observations were performed. In the pig, follicular atresia began with the granulosa cell apoptosis, and the apoptosis of theca intema cells occured at the same time. The apoptosis of granulosa cells initiated randomly within the granulosa cell layer and propagated rapidly into the whole layer. Ultrastructura1ly, apoptotic granulosa cells showed characteristic pyknotic and deformed nucleus and intracytoplasmic vesicles. Apoptotic bodies were eliminated by intact granulosa cells and macrophages. Intact granulosa cells ingested apoptotic bodies transiently, soon after they fell into the apoptosis. Finally, apoptotic bodies and degenerated oocyte were phagocytosed by macrophages. Macrophages entered the ovarian follicle at the time of initiation of granulosa cell apoptosis, and migrated with the progression of apoptosis. By elimination of theca cells, macrophages contributed the completion of follicular atresia These results will provide valuable informations on the study of the interrelation between macrophage and ovarian follicular atresia.

• Journal of Animal Science and Technology
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• v.46 no.4
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• pp.593-602
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• 2004

A feeding trial was carried out to investigate the effect of supplemental complex probiotics on performances, physio-chemica1 properties of meat and inetestinal microflora in broiler chicks. Four hundred eighty broiler chickens, one days old with mixed sexes were fed one of four diets containing 0, 0.1, 0.2 and 0.4% complex probiotics for 7 weeks. There were four replicates with thirty chicks per pen. Diet contained ME 3,100, 3,l00kcal/kg, and CP 22.0, 20.0% for starting and finishing period, respectively. Body Weight gain of chicks fed the complex probiotics tended to increase from the frist week and all complex probiotics higher than control from the 4th week. Chickens fed the diets containing 0.2% probiotics had higher(P<0.05) than those fed the other levels from the 4th week to 5th week. Feed conversion also improved significantly(P<0.05) in the supplemental 0.2% probiotics from the 4th week to 5th week. In physio-chemica1 properties of meat, carcass rate increased significantly(P<0.05) in the supplemental 0.4% probiotics compared to that of control at 7 weeks overall means and abdominal fat pad rate increased significantly(P< 0.05) in the supplemental 0.2% probiotics compared to that of control. Cooking loss decreased significantly(P<0.05) in the supplemental all probiotics. But shear force increased significantly(P<0.05) in the supplemental 0.4% probiotics. The number of ileum and cecum Lactobacillus spp. tended to increase in the supplemental complex probiotics at 7 week of age, but was not significantly different. As the result, supplemental complex probiotics increased performance and physio-chemica1 properties of meat and the number of intestinal Lactobacillus of broiler chicks.

• Journal of Animal Science and Technology
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• v.46 no.4
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• pp.603-612
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• 2004

This study was carried out to investigate the replacing fish meal or soybean meal effect of with feather meal or Bacillus sp. inoculated feather meal in broiler diets on the performances and nutrient utilization. One hundred and eighty broilers were randomly allotted to six dietary treatments(1) control, basal diet; (2) BFMl00, Fish meal replacing at 100% level with bacillus sp. inoculated feather meal; (3) BSM20, Soybean meal replacing at 20% level with bacillus sp. inoculated feather meal; (4) BFMl00+BSM20, Fish meal and soybean meal replacing at 100% level and 20% level with bacillus sp. inoculated feather meal, respectively; (5) GFMl00, Fish meal replacing at 100% level with general feather meal; and (6) GSM20, Soybean meal replacing at 20% level with general feather meal) in a 5 week feeding trial. In overall period, body weight gain of control was the highest(1,623g) and those of BFM 100, BFM 100+ BSM 20 and GFM 100 were 1,572g, 1,564g, and 1,078g, respectively. And that of GFM 100(1,078.3g) was the lowest(p<0.05) among treatments. Digestibility of dry matter for BFM 100+BSM 20(81.46%) was higher(p<0.05) than that for other treatments, and that of crude protein was the lowest (p<0.05). Digestibilities of organic matter were significantly(p<0.05) improved when Bacillus sp. inoculated feather meal was replaced at 100% level with fish meal in the basal diet. Methane and hydrogen sulfide gases from the feces were significantly(p<0.05) decreased in chicks fed the control, BFM 100 and GFM 100 diets, when observed after 3 weeks of feeding trials. Feed costs of the control and BFM 100 were 417 and 384 won, respectively but that of BSM 20 was 558 won. Therefore, replacement of fish meal with Bacillus sp. inoculated feather meal in the diets for chicks could be useful for economic production.

• Journal of Animal Science and Technology
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• v.45 no.1
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• pp.113-122
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• 2003

Net and metabolizable energy requirements for maintenance of Hanwoo (Korean native cattle) bulls were estimated in twenty-eight fasting metabolism trials using seven different feeds at four stages of body weight(100, 200, 300 and 400kg). Three cattle for each of twenty-eight trials fed at a level of maintenance energy requirement were housed in metabolic stalls during the 5 days of collection period. Thereafter, during the 2 days of respiration period the heat production was measured by indirect calorimetry using respiratory chamber. After finishing the respiratory metabolism trials under the maintenance level, experimental animals were fasted for 5 days and were measured heat production by indirect calorimetry using respiratory chamber. Seven different feeds were: 1) mixed ration of concentrate and rice straw, 2) mixed ration of concentrate and mixed grass hay, 3) mixed ration of concentrate and corn silage, 4) rice straw alone, 5) mixed grass hay alone, 6) corn silage alone, 7) concentrate alone. Fasting heat production were 66.05/$W^{0.75}$ at 100kg of body weight and 60~63kcal/$W^{0.75}$ at 200~400kg of body weight. When subtracting heat loss by muscular work from the fasting heat production, basal metabolic rate was 55.92kcal/$W^{0.75}$. The average values of NEm requirements were obtained by adding urinary energy excretion to the basal metabolic rates were 69.1, 62.1, 65.8 and 64.4kcal/$W^{0.75}$ for the four stages of body weight, respectively. The ME requirement for maintenance could be calculated using retained energy and the efficiency of utilization of ME for net energy. The ME requirement for maintenance thus obtained was 102.69kcal/$W^{0.75}$.

• The Korean Journal of Pain
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• v.1 no.1
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• pp.106-119
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• 1988

For the relief of pain in 3 cases of whiplash syndromes (case I, II and IV) and in one of reflex sympathetic dystrophy (case III), we have carried out six intentional. total spinal blocks (TSB) which attempted two times in case I, three in case II and one in carte III whoso various symptoms were chronically unresponsive to the usual conservative treatments, and a time of cervical epidural and right suprascapular nerve block in case W whose acute symptom lasted 4 drys following the cervical injury (see fables from 1 to 9). During the 753, we have observed clinically the sequential charges of respiration, lid and pupil reflexes, body motion and consciousness. And checked the blood pressure, pulse rate and arterial Pco2. The effectiveness of those blocks has been assessed by using the Visual Analog Scale which is designed to measure the patient$\acute{s}$ subjective intensity of pain and also we have found out the sequelae following those blocks. The methods of the blocks were as the following: 1. Under the N.P.O. for 8~10 hours, the preparations of immediate cardiopulmonary resuscitation and premedication with atropine 0.5mg at thirty minutes before the TSB, it was performed by injecting the mixture of 2% mepivacaine 10 or 15ml and normal saline 10 or 5ml through No. 23 G. spinal needle into the subarachnoid space of $C_7-T_1$ interspinous region with fully flexed neck on the lateral posture. Immediately after the injection of the local anesthetic in the lateral position, the patient$\acute{s}$ were hasten to change Trendelenburg$\acute{s}$ position in order to act the drugs cephalad and to make easy controlled respiration with oxygen. 2. The cervical epidural block was done by injecting the mixture of 0.5% bupivacaine 4ml, normal saline 4ml and triamcinolone 15mg through No. 18 G. Tuohy needle into the epidural space on the same region and posture as the above without premedication.7he suprascapular nerve block was done by injecting of 0.5% bupivacaine 3ml only into the right suprascapular fossa on the sitting posture. The results were as the following: 1. The cessation of respiration was seen within 5 minutes following the subarachnoidal injection of the above 20ml mixture in 2 to 3 minutes and then soon the consciousness began to disappear. The loss of Lid and pupil reflexes noted between 5 to 10 minutes and the size of the dilated pupils was equal between 5 to 20 minutes, but the pupil of the dependent side on tile lateral position was dilated 1 to 3 minutes earlier than that of the independent. The patients had r=ever responded to any stimulations during the TSB except their heart funtion. 2. The recovery of the TSB was as the following, firstly the ankle and lower limb of the independent side began to move slightly with in 34 to 75 minutes after the injection and then that of the dependent Secondly the neck and upper limb moved 6 to 15 minutes later than the lower limb. Thirdly the self respiration began to appear between 40 to 80 minutes from the block. The lid and pupil reacted to touch and light respectively between 40 to 80 minutes but the pupil of the independent side responded earlier than that of the depends. Lastly the consciousness recovered completely between 80 to 125 minutes from the block. 3. In the cardiopulmonary function during the TSB, the blood pressure were stable except the 210/130 tory at the and block of case I. There were bradycardias between 65 to 85 minutes in case I and II but no arrythmia on the EKG. The level of the arterial Pco2 was maintained to 43~45 torr during the TSB. 4. The effectiveness of the above blocks was no pain(0%) in case IV, and light (10~20%) in case I and II but no improvement in case III. 5. The right arm weakness has been complicated as to be Injected accidently the "COLD" local anesthetic at the End block of case I.

• Applied Biological Chemistry
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• v.37 no.3
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• pp.143-147
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• 1994

Four samples of commercially manufactured yogurts (plain, drinking type) were purchased and evaluated their physico-chemical properties, buffering capacity. And the survival rate of lactic acid bacteria and their ${\beta}-galactosidase$ activity under the acidic conditions (in vitro) were investigated. The values of pH, titratable acidity, viscosity and viable cell counts of yogurts were $3.71{\sim}4.08$, $0.990{\sim}1.045%$, $256{\sim}3164\;cps.$ and $10^8{\sim}10^9\;cfu/ml$, respectively. The volume of 1.0 M-HCl required to reduce the pH of yogurt (50 ml) to minus 2 value was $3.58{\sim}4.33\;ml$. When commercial yogurts were incubated at $37^{\circ}C$ for 120 minutes under the acidic conditions (pH 3.5, 2.5, 1.5), the survival rates of lactic acid bacteria in yogurt were $3.5{\times}10^{-2}{\sim}3.6{\times}10^{-1}%$ at pH 2.5, $8.3{\times}10^{-5}{\sim}4.2{\times}10^{-3}%$ at pH 1.5, respectively, but there was no significant difference at pH 3.5. The remaining activities of ${\beta}-galactosidase$ were $9.4{\sim}36.2%$ at pH 2.5, $4.2{\sim}19.0%$ at pH 1.5, respectively. These results suggested that a significant number of lactic acid bacteria in yogurt might be destroyed in the hostile environment of the stomach, but ${\beta}-galactosidase$ activity from yogurt might be somewhat maintained probably due to the protecting effect by its cell wall and membrane.

• Economic and Environmental Geology
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• v.43 no.4
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• pp.333-341
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• 2010

Fluvial sediments are widely distributed in present and old river-beds of the mid-Keum River, the tributaries of which are the Yugu and Jeongan Rivers. The basement of the mid-Keum River area consists of Mesozoic granites which are easily eroded compared to Precambrian gneisses, which are exposed in the upper-Keum River area. The provenance of the fluvial sediments includes both the Precambrian gneisses and Mesozoic granites, which occur in the catchment of the mid-Keum River. The coarse-grained sediments were probably transported from the river-beds and the overbank floodings of the main Keum River and its tributaries when the climate was warm and wet. The oldest mud deposits were dated at ca. 9,400 yr BP by the radiocarbon method. It has been estimated that the sand deposits below the dated muds were formed in a period from the Late Pleistocene to the Early Holocene. However we have revealed that the major part of the present river-bed sediments was formed at ca. 3,000-6,000 yr BP, i.e., in the mid- to late Holocene, when summer monsoon was very strong due to climatic changes. We have calculated fluvial sedimentation rates of 0.12-0.16 cm/yr and 0.02-0.09 cm/yr for borehole KJ-29 river-bed sediments and borehole KJ-28 floodplain deposits, respectively. We conclude that the sedimentation rate is higher near the present stream channel than near the floodplain.

• Korean Journal of Environmental Agriculture
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• v.2 no.2
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• pp.98-102
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• 1983

The hydrogen fluoride gas generated from making the cement hardener injured the plants growing at the neighbour field. This investigation was conducted on sample analysis of hydrogen fluoride gas damage plants which included the ratios of destroyed leaves, damage symptoms, and nutrient elements in paddy rice and weeds. The results obtained were as follows; 1) The ratio of destroyed leaves at near HF source was very high reaching about 95% at 100m, 65% at 500m, 5% at 2㎞, respectively. 2) The necrosis was the characteristic symptom of fluoride injury on rice plant and occurred predominantly at the tip and margins of damage leaf. It developed along the tip and margins of leaves with a dull, gray-green, water soaked discoloration. 3) The fluorine content of tip and margins of damaged rice leaves were 1,600 ppm, 3 to 20 times higher than that of center part and it ranged from 130 to 242.5 ppm in weed leaves, but from 10 to 15 ppm in normal weed leaves. 4) The contents of calcium, potassium, silicon, iron and manganese were higher in tip and margins than in the center of rice damage leaves. 5) The Cocculus trilolous. D.C was the most resistant plant to HF gas than any other plant growing in this site, while wild berry and aralia tree belong to most sensitive plant group.

• Korean Journal of Environmental Agriculture
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• v.25 no.2
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• pp.147-156
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• 2006

This study was conducted to investigate natural distribution of aquatic plane and to find out natural aquatic plants which highly absorb nutrient N and P. We surveyed vegetation within ${\pm}2m$ from streamside in 12 tributaries of the West Nakdong river watershed during May to October in 2003. Hydrophytes surveyed in tributaries of the West Nakdong river watershed were 27 families, 61 genera, 76 species, 3 varieties. Major dominance species of aquatic plants were Z. latifolia, P. communis, P. thunbergii, P. arundinacea, P. japonica, and P. distichum var. indutum. Aquatic plants having high production ability of biomass were Z. latifolia, P. communis, P. arundinacea, P. japonica, and E. crus-galli var. echinata. In the vertical distribution of hydrophytes within streams, dominant species were P. thunbergii and P. japonica in the upper stream, but dominant species in the downstream were P. communis and Z. latifolia. Species diversity or aquatic, plants was reduced, but their biomass and nutrient (T-N and T-P) content per the natural area unit $(m^2)$ were increased in the downsteaam. Nutrient N and P content of aquatic plants per the natural area unit were high at Joman river, Pyeonggangcheon, Bulam drainage canal, and Hogyecheon. Fifty-seven species of aquatic plants having high biomass were grounped into 4 categories $(I{\sim}IV)$ according to their nutrient content per dry weight unit. I group $(T-N,\;\geqq20gkg^{-1}\;&\;P_2O_5,\;\geqq7gkg^{-1})$ was comprised of 3 submerged plants (H. verticillata, P. crispus, and C. demersum), e emergent plants (O. javanica, P. distichum var. indutum, and R. sceleratus), 1 suspended plant (T. japonica), and 1 riparian plant (A. lobatum). Otherwise, in classification of natural hydrophytes according to their nutrient content per natural area unit, Z. latifolia, P. communis, P. longiseta, P. arundinacea, and P. distichum var. indutum possessing great biomass productivity as emergent plants were included in I group $(T-N,\;\geqq1gm^{-2}\;&\;P_2O_5,\;\geqq0.7gm^{-2})$.