• Animal Systematics, Evolution and Diversity
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• v.4 no.2
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• pp.91-102
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• 1988

Both Cobitis lutheri Rendahl and C.striata Ikeda previously regarded as the subspecies of C.taenia are revised here and raised to the species rank based on the distinct color pattern on their body sides in relation to the shpae of lamina circularis and suborbital spine, and distinct distributional patter. C. lutheri was similar to C. striata in chromosome number and karyotype, but chromosomal polymorphism as Robert sonian event was confirmed only in the population of C.lutheri studies. Both, C. kutheri and C..striata have disjunct ranges : the former in western Korea and east-northern China Mainland, the latter in the Smjin River of korea and west-southern Japan. hybridization between C. lutheri and C. striata by secondary contact appeared in the limited zone of the Dongjin River, Chllabuk-do province, korea, but the evidence for habitat segregation between them suggests the possibility that natural hybridization occurs between the two species and introgression results. We consider that the two species were produced as ecological equivalent species in the different branch stream of the Paleo-Hwangho River , The time of recession of sea level during the gracial period.

• Horticultural Science & Technology
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• v.18 no.2
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• pp.103-106
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• 2000

The genetic relationship of Bletilla striata native to Korea and Japan was investigated using random amplified polymorphic DNA (RAPD). The 156 reproducible DNA bands, consisted of 58 polymorphic and 98 monomorphic bands, were obtained by polymerase chain reaction (PCR) with selected 10 random primers. The 8 Bletilla lines have been classified into three groups according to the similarity coefficient obtained by RAPD analysis. The dendrogram showed overall correlationship between similarity coefficient of 0.48 and 0.84. The first group included A (Bletilla striata native to Korea), B (Bletilla striata variegated and native to Korea in Mokpo), C (Bletilla striata variegated and native to Korea), D and E (Bletilla striata native to Japan). In this group, it was showed that B and C had the most similar genetic relationship. The similarity coefficient between D and E was 0.77. D and E had a very close resemblance in plant height and flower color with A native to Korea, respectively. The second group included only G (dwarf Bletilla native to Japan) and had a different morphological character compared to other cultivars. The last group included F and H (dwarf and variegated Bletilla native to Japan) and they had a similarity of variegation.

• Korean Journal of Ichthyology
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• v.9 no.1
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• pp.121-129
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• 1997

The five species of genus Cobitis from Korea were investigated by electron microscopes to clarify the adhesive membranes on zona radiata. In the late vitellogenic stage the adhesive membranes could be classified into two form as follows: 1) granular form of Cobitis lutheri, C. striata, C. sinensis, and C. sinensis-longicorpus, 2) villous form of C. melanoleuca. Although C. lutheri, C. striata, C. sinensis, and C. sinensis-longicorpus possessed the same granular form, it was evident that fine structure of the zona radiata varied according to species. The adhesive membranes and fine structures of zona radiata in Cobitis showed a species specificity closely related to their habitats and spawning properties.

• Animal Systematics, Evolution and Diversity
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• v.13 no.1
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• pp.29-36
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• 1997

한국산 기름종개속 어류중 Cobitis taenia complex의 집단간 유전적 차이에 따른 종 분화 여부를 밝히고자 6개 집단을 대상으로 mitochondrial DNA(mtDNA)의 RFLP분석을 실 시하였다. C. taenia complex mtDNA를 10개의 6-base cutting 제한효소로 처리한 다음 그 절편 양상을 비교, 분석한 결과 6개 집단 공히 mtDNA 의 전체 genome 크기는 약 17.0$\pm$ 0.5Kbp였으며 공동절편수(F)에서 C. t. taenia 2개집단과 C. t. stria와 C. t. lutheri 4개 집단 간의 F값은 평균 0.263으로 차이가 있었으나, C. t. striata 와 C. t. lutheri 사이는 F=0.569로 가깝게 나타났다. 염기치환율 (p)에 있어 C. t. taenia는 C. t. striata 및 C. t. lutheri와 평균 p=0.082로 뚜렷한 종간차이를 보였으나, C. t. striata와 C. t. lutheri 집단들은 p=0.033으로 매우 가까운 유사성을 나타내었다. MtDNA 분석결과 C. taenia complex 중 C. t. taenia는 완전히 종분화가 이루어진 별종으로, C. t. striata와 C. t. lutherisms 아직 종수준의 분화가 이루어지지 않은 아종으로 분류함이 타당하다고 사료된다.

• Korean Journal of Ichthyology
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• v.8 no.1
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• pp.1-8
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• 1996

Cobitis striata testes were paired, with elongate form and suspended on the dorsal body wall. The testicular structure of immature testes composed of many seminal lobules with regular arrangement, whereas mature testes anastomose neighbouring seminal lobules. The spermatozoa of C. striata were anacrosomal aquasperm type and have spherical nucleus. The mid piece of spermatozoa was $0.8{\mu}m$ in length and contained 5~8 ring-shaped mitochondria. The flagellum of the spermatozoa in the present species was uniflagellate consisting of a typical 9+2 axoneme without fins.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.19 no.3
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• pp.257-264
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• 1986

The Chromosomes of five cobitid fishes, Cobitis taenia taenia, C. taenia lutheri, C. taenia striata, C. longicorpus and C. koreensis were studied. The karyotypic studies were based on the observations from the flame-drying preparations. The results obtained such as the number of somatic chromosomes, the type of chromosomes according to the centromeric loci and the number of chromosomal arm(AN) were as follows: C. longicorpus, 2n=50, 12m-8sm-30a, AN=70; C. koreensis, 2n=50, 10m-12sm-28a, AN=72; C. taenia taenia, 2n=48, 14m-4sm-30a, AN=66: C. taenia lutheri, 2n=50, 10m-6sm-34a, AN=66; and C. taenia striata, 2n=50, 10m-6sm-34a, AN=66. Peculiarly, in the case of C. taenia lutheri the chromosome number of somatic diploid was found to be 48-51, however, the number of chromosomal arm was 66, irrespective of the difference in the numbers of each somatic genome. It was confirmed there exists the Robertsonian event, one of the chromosomal polymorphism in C.t. lutheri. It was remarked taxonomically that the karyotype of C. taenia taenia of Korea having 48 diploid chromosomes was not identical with that of Europe and Japan with 50 chromosomes. Based on the karyotype analysis the Korean cobitid fishes can be classified roughly into three species groups according to arm numbers and diploid numbers; 1) C. taenia taenia, C. taenia lutheri, C. taenia striata 2) C. koreensis, C. longicor pus, C. rotundicaudata and 3) C. granoei.

• KSBB Journal
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• v.6 no.2
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• pp.201-205
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• 1991

We have investigated influencing factors on viability of Bletilla striata protoplasts electroporated in the presence of various electrical conditions. Cultures of embryogenic callus and embryogenic cell suspension were established with immature seeds of Bletilla striata. Viabilty of electroporated protoplasts was decreased according to the increaseing of electroporation voltage and capacitance. An optimal condition of electroporation for viable protoplasts was in HBM buffer at $4^{\circ}C$.

• The Korean Journal of Ecology
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• v.17 no.2
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• pp.149-157
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• 1994

To investigate the causes of distribution of Kummerowia striata (Thunb.) Schindl. mostly restricted on the roadside and reclaimed land, plant growth was analyzed under different of tread-pressures: $P_1$(10 times/day, $16.34Kg/cm^2$), $P_2$(20 times/day, $31.52 Kg/cm^2$), $P_3$(30 times/day, $40.79 kg/cm^2$) and the control $P_c$(0 times/day, $3.73 Kg/cm^2$). The matter production in the $P_1$decreased compared with that in the $P_c$, and those in the $P_2$and $P_3$ continuously decreased so that its length growth gradually decreased and, in particular, the growth of leaves and nodule was suppressed. The number of leaves and roots tended to increase in the $P_2$and $P_3$. The water contents of roots following the increase of tread-pressure increased continuously. Following the increase of tread-pressure, T/R ratio and C/F ratio tended to increase. Kummerowia striata showed the highest growth in the $P_c$ and showed 26% relative growth in the $P_1$compared with that in the $P_c$. This plant showed 8% relative growth in the $P_3$, which is excess to the tread-pressure $21Kg/cm^2$, the limit of growth in the plant. Because this plant grew continuously in the P$_3$, the tread-pressure is regarded as an important factor affecting the roadside distribution of this plant.

• Animal Systematics, Evolution and Diversity
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• v.9 no.2
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• pp.151-170
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• 1993

Morphometric, band-pattern and electrophoretic analysis on Cobitis taenia complex were performed to investigate the morphological and genetic differentiation and to clarify their taxonomic status. Intermediate types of band-pattern (C and D type) were more frequently expressed than that of types of C. t. taenia(type A) and C. t. lutheri (type B). Sexual dimorphism of band-pattern was observed not only in C. t. taenia(type A) and C. t. lutheri(type B). Sexual dimorphism of band-pattern was observed not only in C. t. lutheri but also in C. t. taenia and C. t. striata as well. Discriminant function analysis based on 19 morphological characters shows no significant differences among C. taenia complex. The degree of genic variation of C. t. striata was higher ( =1.48, P=31.2%, HD=0.009) than those of C. t. striata was higher( =1.48, P=31.2%, HD=0.082 and HG=0.009) than those of C. t. lutheri ( =1.37, P=2.7%, HD=0.058 and HG=0.065). The average genetic similarities between C. t. lutheri and C. t. taenia-C. t. striata were S=0.62 and S=0.66 respectively and these values indicate that C. t. tanenia has evolved specific level of differentiation. C. t. striata and C. t. lutheri show subspecifc level of close genetic similarity (S=0.82). Based on the divergent time estimate (Nei, 1975) it is assumed that C. t. tanenia was branched off from the other subspecies about two million years before present (MYBP) and C. t. striata and C. t. lutheri were differentiated about 0.6 MYBP. The use of C. sinesis an the scientific name for the Korean C. t. taenia, proposed by Kim and Lee (1988) seems incorrect since they are quite different in the structure of lamina circularis (Vladycov, 1935), the external morphology and distribution (Cheng and Zheng, 1987) and the chromosome number(Yu et al., 1989). Kim and Lee(1988) also argued that C.t. striata and C. t. lutheri should be treated as distinct species but the present study and other reports (Kim and Lee, 1984; Kim and Yang, 1993) do not support it. We conclude that C. t. taenia is a good species and C. t. striata and C. t. lutheri are subspecific status. Their scientific names should be revised in the future.

• Journal of Pharmacopuncture
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• v.26 no.1
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• pp.44-52
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• 2023

Objectives: Dental caries is one of the most prevalent human diseases worldwide. The disease initiates with bacterial adherence to the tooth surface followed by the formation of dental plaques. Mutans streptococci and Candida albicans are principal oral microorganisms involved in the initiation and development of dental caries. Phytochemicals have been shown to possess promising antimicrobial properties against a wide range of microorganisms and can be used for the prevention and treatment of dental caries. Herein, we reviewed literature on plants that are traditionally used for their antimicrobial properties or possess promising anticariogenic activity. We selected aerial parts of Scrophularia striata (S. striata) and galls of Quercus infectoria (Q. infectoria) and investigated their antimicrobial activity against cariogenic microorganisms. Methods: Water soluble fractions were obtained from hydroalcoholic extracts of S. striata and Q. infectoria and their antimicrobial activity against Streptococcus mutans (S. mutans), Streptococcus sobrinus (S. sobrinus), and Candida albicans (C. albicans) was evaluated separately and in combination. The extracts were then used for preparing an herbal mouthwash whose stability and tannic acid content were evaluated over 60 days. Results: Q. infectoria gall extract possesses efficient antimicrobial activity that was synergistically enhanced in the presence of S. striata extract. Mouthwash prepared using these extracts showed desirable organoleptic characteristics, antimicrobial activity, and stability. Conclusion: Extracts of S. striata and Q. infectoria galls can be used together for preparing dental products with effective anticariogenic properties. Our study highlights the importance of extensive pharmacological investigations when using herbal products alone or in combination with other chemical substances.