• Title/Summary/Keyword: C. pisifera

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Efficient Extraction Methods and Analysis of Essential Oil from Softwood leaves (침엽수 잎으로부터 효율적인 정유 추출법 탐색 및 정유성분 분석)

  • 양재경;강병국;김태홍;홍성철;서원택;최명석
    • KSBB Journal
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    • v.17 no.4
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    • pp.357-364
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    • 2002
  • For the effecient extraction methods of essential oil, pretreatment of leaves, ratios of water and leaves, extraction time, and collection season from the Chamaecyparis obtusa and Chamaecyparis pisifera leaves were studied. The chemical composition of essential oil was analyzed by GC-MS. The yield of essential oil from ground leaves was higher than that of chopped leaves. The yield of essential oil was not affected much by mixing ratios of water and leaves. The yield of essential oil reached maximum after 5 hours. The content of essential oil of C. obtusa leaves collected during winter was 4.5%, whereas the content of essential oil of C. pisifera collected during fall was 5.3%. The composition of essential oils extracted form C. obrusa and C. pisifera was different. The major constituents in the essential oil of C. obtusa were monoterpene as limonene, terpinene-4-ol, ${\gamma}$-selinene, and a-cedrene, and those of C. pisifera was monoterpens as ${\alpha}$-pinene, myrcene, limonene, bornyl acetate, ${\beta}$- caryophyllene, longifolene, and ${\beta}$-cedrene.

A Study on the Extractives of Domestic Major Softwood Needles (II) - Antioxidant Activity of the Extractives from the Needles of Chamaecyparis pisifera (Sieb. et Zucc.) Endlicher - (국내산 주요 침엽수 잎의 추출성분 (II) - 화백나무 (Chamaecyparis pisifera (Sieb. et Zucc.) Endlicher)잎 추출성분 및 항산화 활성 -)

  • Lee, Sang-Keug;Bae, Young-Soo
    • Journal of the Korean Wood Science and Technology
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    • v.34 no.4
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    • pp.76-82
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    • 2006
  • The dried ground needles (2.0 kg) of Chamaecyparis pisifera (Sieb. et Zucc.) Endlicher were extracted with acetone-$H_2O$ (7:3, v/v), concentrated, and fractionated with a series of n-hexane, methylene chloride, ethyl acetate and water on a separation funnel. Each fraction was freeze dried, then a portion of ethyl acetate soluble powder was chromatographed on a Sephadex LH-20 column using a series of aqueous methanol and ethanol-n-hexane mixture as eluents. The isolated compounds were identified by cellulose TLC, $^1H$-, $^{13}C$-NMR, COSY, HETCOR, FAB and EI-MS. (+)-catechin, taxifolin-3-O-${\beta}$-D-xylopyrano-side, quercetin-3-O-${\alpha}$-L-rhamnopyranoside were isolated from the ethyl acetate soluble fraction of Chamaecypairs pisifera needle. Antioxidative tests on the isolated compounds indicated that all of the compounds showed similar values to ${\alpha}$-tocopherol and BHT as controls.

Composition of Essential Oils from the Leaves and the Fruits of Chamaecyparis obtusa and Chamaecyparis pisifera (편백과 화백의 잎과 열매에서 분리한 정유성분의 조성)

  • Hong, Chul-Un;Kim, Cheol-Sang;Kim, Nam-Gyun;Kim, Young-Hoi
    • Applied Biological Chemistry
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    • v.44 no.2
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    • pp.116-121
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    • 2001
  • The compositions of essential oils isolated from the leaves and fruits of Chamaecyparis obtusa (Sieb. et Zucc). Endl. and Chamaecyparis pisifera (Sieb. et Zucc.) Endl. were analyzed through GC and GC-MS. The oil yields were 0.83% (as fresh weight) and 1.36% in the leaves and the fruits of C. obtusa, and were 0.92% and 1.28% in those of C. pisifera, respectively. More than 90 components were identified, including high contents of monoterpenoids and sesquiterpenoids. Contents of monoteipenoids in the leaf and fruit oils of C. pisifera were higher than in those of C. obutsa. The major constituents in the leaf oil of C. obtusa were sabinene (11.81% as determined through GC peak area), limonene (7.73%), bornyl acetate (6.92%), $borneol+{\alpha}-teirineol$ (15.67%), and elemol (12.82%), and those in the fruit oil were myrcene (8.12%), ${\gamma}-terpinene$(5.91%), p-cymene(7.62%), $borneol+{\alpha}-terpineol$(6.53%) and ${\beta}-caryophyllene$ (23.74%). The major constituents in the leaf oil of C. pisifera were ${\alpha}-pinene$(32.34%), ${\delta}-3-carene$(25.28%), myrcene(11.72%), and bornyl acetate (8.77%), and those in the fruit oil were ${\alpha}-pinene$ (29.38%), ${\delta}-3-carene$(30.27%), myrcene(15.05%), and limonene(8.10%).

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A Study on the Community Structure of Vegetation Landscape in Naejangsan National(I) (내장산국립공원 식물경관의 군집구조에 관한 연구(I))

  • 이규완;심경구
    • Journal of the Korean Institute of Landscape Architecture
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    • v.21 no.2
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    • pp.50-67
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    • 1993
  • This study analyzed the distribution and structure of the vegetation landscape in Naejangsan National Park. The plant distribution was investigated using a field survey. There were 72 sites sampled using the clumpled sampling method. The data derived from the investigation was analyzed using the quantitative analysis of Bray-Curtis method. The analysis was performed by the classification of TWINSPAN. The ordination of DCA and RA was used for the species composition and successional trends. The results are as follows; 1. Quercus. serrata-Q. variabilis community as 21.76(16.49$\textrm{km}^2$), was the largest community in the actual vegetation of the Naejangsan National Park. The degree of green naturality index 8 area covered 64.8% of the study area and the index 6 area did 16%. 2. Classified by the ordination of DCA and environmental variables, such as the plant community was divided into seven groups according to the altitude and forming groups; Chamaecy Paris. pisifera-P.densiflora community, P.densiflora community, Q.variailis community, T.nuciofera-A.palmatum community. 3. Ther species diversity and evenness indices of C.pisifera-P.densiflora community, P.densiflora community appeared low but C.laxiflora communitywhich was the most stable community in the study area. 4. The similarity indices between Q.variabilis community and Q.serrata-Q.variabilis community were calculated 58.84%, but those between other communities were comparatively low level. 5. The successional trends of DBH class seem to be from C. pisifera-P.densiflora community, P.densiflora community to Quercus species community and from Q.variabilis community, Q.serrata-Q.variabilis community to Carpinus species community. 6. Results suggested that the successional trends in Naejangsan National Park; P.densiflora community\longrightarrowQ.variablilis community, Q.serrata-Q.variabilis community\longrightarrowC.laxiflora community in sequence.

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Studies on Biological Activities of Woody Essential Oils( I ) - Antimicrobial Activity of Essential Oils from Coniferous Needles - (수목 정유의 생리활성에 관한 연구( I ) - 침엽수 잎 정유의 항균활성 -)

  • Lee, Sung-Suk;Kang, Ha-Young;Choi, In-Gyu
    • Journal of the Korean Wood Science and Technology
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    • v.30 no.1
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    • pp.48-55
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    • 2002
  • The essential oils were extracted from the needles of several softwood tree species, Pinus densiflora, Pinus koraiensis, Chamaecyparis obtusa and Chamaecyparis pisifera to investigate their biological activities. The antifungal activity by hyphal growth inhibition method was determined by three wood rot fungi, one mouldous fungi, and three tree pathogenic fungi and the antibacterial activity by paper disc method and turbidiometric method by three gram positive bacteria and one gram negative bacteria, Escherichia coli, were also tested. Antifungal activity was the best in the essential oil from C. obtusa, and the following was resulted in order of P. densiflora and P. koraiensis. The essential oil from P. densiflora showed higher antifungal activity against wood rot fungi than that from P. koraiensis while the result was contrary against tree pathogenic fungi. However, the antibacterial activity was the best in the essential oil from P. densiflora, and the following was in order of C. obtusa and P. koraiensis. From these results, the variation of biological activity seemed to be due to the chemical composition of essential oils.

Soil Chemical Property, Mortality Rates and Growth of Planting Trees from Soil Covering Depths in Coastal Reclaimed Land of Asan Area (아산지역 해안매립지의 복토높이에 따른 토양화학성, 수목 고사율 및 생장 특성)

  • Byun, Jae-Kyeong;Kim, Choon-Sig;Lim, Chae-Cheol;Jeong, Jin-Hyon
    • Korean Journal of Soil Science and Fertilizer
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    • v.44 no.3
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    • pp.502-509
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    • 2011
  • It is important to determine optimum soil covering depths for tree survival and growth because soil covering depths for establishing tree planting bases in coastal reclaimed lands are related to the costs for soil collection, transportation and land reclamation. The objectives of this study were carried out to determine optimum soil covering depths for the normal growth of planted trees in a coastal reclaimed land. The study sites were located in Asan National Industrial Complex in Pyeongtaek City, Gyeonggi-do. Four tree species (Pinus thunbergii, Chamaecyparis pisifera, Zelkova serrata, Quercus acutissima) with one hundred eighty trees of each species were planted in various depths of soil covering (no soil covering, 0.5 m, 1.5 m, 2.0 m soil covering treatments) on April 1998, and the tree growth patterns were measured on September 2000. The change of soil properties, tree mortality rate, root collar diameter and height growth were measured from each soil covering depth treatment on September 2000. Soil pH, EC, exchangeable cations ($K^+$, $Na^+$, $Ca^{2+}$, $Mg^{2+}$), anion $Cl^-$, and base saturation increased with decreased soil covering depths. The mortality rates of tree species showed decreased with increased soil covering depths. The height growth of tree species increased with increased soil covering depths. Height growth of Pinus thunbergii was significantly different between the soil covering depth below 0.5m and other three covering depths, while the growth of other species (C. pisifera, Z. serrata, Q. acutissima) was significantly higher in soil covering depths below 1.5 m than in other soil covering depth treatments. The root collar diameter growth of all tree species showed increasing trends with increased soil covering depths. It is recommended to cover the soil depths above 1.5 m to decrease mortality and to stimulate the tree growth of C. pisifera, Z. serrata and Q. acutissima, while P. thunbergii which is a salt tolerate species could be planted in the 1.0 m soil covering depth.

A Study on the Community Structure of Vegetation Landscape in Naejangsan National Park(II) (내장산국립공원 식생경관의 군집모형에 관한 연구(II))

  • 이규완;심경구
    • Journal of the Korean Institute of Landscape Architecture
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    • v.22 no.1
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    • pp.85-100
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    • 1994
  • This study was to analyze the relationship between the community or species and environmental variables of the vegetation landscape in Naejangsan National Park. The analysis was performed by the ordination of DCA and CCA. The study was to compare and analyze the species composition status by the layer and the growth characterisics of the following vegetations ; Torreya nucifera community, Quercus variabilis - Acer palmatum community, Q. variabilis community, Q. variabilis - Sasa purpurascens community and Daphniphyllam macropodum community. The results are as follows; 1. The result of the study on the relationshkp between the stand scores of DCA ordination and environmental variables showed that the soil pH, the ammount of AV-P2O5 and the ammount of C.E.C. tend to increase while Pinus densiflora community changes to Q. variabilis - Q. serrata community and finally Carpinus laxiflora species community siginigicantly. The relation between the stand scores of CCA ordination and several enviromental variables suggested that the species of C. laxiflora and C. tschonoskii the species of Q. cariabilis and Q. serrata the species of C. pisifera, P. densiflora and Q. aliana in sequence grows in more fertile soil. 2. As the result of the analysis of the T. nucifera community showed, reproduction did not increase, and the characteristic of growth was not affected. The result is shown in the growth curve that was damaged by the climate and the human interference. 3. The A. palmatum was found to be as minor species in the middle layer and the crown areas did not have sufficient crown. 4. The result of the analysis of the relationship between Q. variabilis community and Q. variabilis - S. purpurascens community showed a decreasing tendency in the growth and number of species. 5. D. macropodum which constituted the dominance species in the middle layer had a nomal growth curve, and then the successional trend of D. macropodum species seems to be located in the climax species.

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The Flora of Geonjisan, Jeonju (건지산(전주)의 식물상)

  • 김계환;박준모;임성구;황영희;조성종;김상용
    • Journal of Korea Foresty Energy
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    • v.20 no.1
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    • pp.12-27
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    • 2001
  • This study was carried out to investigate the vascular flora at Geonjisan, Jeonju, Jeollabuk-do and the findings are followings (1) The vascular plants identified and surveyed were 359 taxa(306 species, 49 varieties and 4 forms of 245 genera of 94 families) at the study sites. (2) There were 144 taxa (125 species, 15 varieties and 4 forms of 91 genera of 49 families) for the woody plants, while 215 taxa(181 species and 34 varieties of 157 genera of 54 families) were identified for the herbaceous plants. Twenty-nine taxa (28 species and 1 variety of 22 genera of 12 families) were investigated for the naturalized plants at the study sites. (3) Currently existing dominant vegetation forming the major trees layer at the study sites were Robinia pseudoacacia, Chamaecyparis pisifera, C. obutusa, Torreya nucifera, Castanea crenata and Acer buergerianum for the planted species, and naturally growing species forming the major trees layer were Quercus acutissima and Q- serrata.

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The Formative Characteristics of Seogo-jeongsa & Sameun-jeong Byeolseo Gardens in Toerori Miryang (밀양(密陽) 퇴노리(退老里) 서고정사(西皐精舍)와 삼은정(三隱亭) 별서(別墅)의 조영(造營) 특성(特性))

  • Lee, Hyun-Woo;Rho, Jae-Hyun
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.31 no.4
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    • pp.70-83
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    • 2013
  • To widen prospect of villa as Korea traditional garden, the result of the research about constructional characteristics of Seogo-jeongsa and Sameun-jeong of Yeoju Lee family at Toero-ri, Miryang-si, Gyeongsangnam-do, Korea, is abstracted as below. Seogo-jeongsa(西皐精舍) and Sameun-jeong(三隱亭) was intended to practise retirement with sense of unity with the head residence, since Seogo-jeongsa was 340m far and sameun-jeong was 630m far away from head residence, which set within 1km. Although Seogo-jeongsa's basic prop, Sameun-jeong's timber, which are both from designer's pen name and head residence name, "Hangjae(恒齋)" and "Yongjae(庸齋)," and Hanseoam have each different exposure, it is homogeneous as space configurational side in order to optimize the gaze leading effect and appreciated domestic trees from the pond, which is center of the garden and the significant facility. Method of direction of Seogo-jeongsa's Hwalsudang(活水塘) and Sameunjeong's pond of Sameun-ji(三隱 池) gets attention by distinction. Especially, directing of 3 step water flow from behind to front and traditional pond method, called Bangji-Bangdo(方池方島), were very unique that any other place can be found. Also, the middle islet on the pond at both villa, which isn't more supper, but more for the symbolic molding that seek a stone pagoda(石假山), is more interesting directing method as considering the relationship of the 3 stone pagoda imageries on the surface of Ghwayukchon(果肉泉) At the same time, Seogo-jeongsa and Sameun-jeong shows very different characteristics from traditional villa that adopted both domestic trees and foreign trees. Particularly, it is extremely exceptional case to plant vigorous needleleaf tree, such as Chamaecyparis pisifera, C. obtusa, Pinus bungeana, P. palustris, Sciadopitys verticillata, Metasequoia glyptostroboides and Cedrus deodara, on Sameun-jeong. Moreover, adopting foreign wild needleleaf trees for landscaping trees, such as a Torreya nucifera, Taxus cuspidata, P. parviflora, and foreign landsacping trees, such as P. bungeana, Cryptomeria japonica and C. obtusa tells planting trend of the late Joseon dysnasty era. Also, as we can know from 2 Jipgyeong(集景), which is 'Seogo-jabyoung 17 young(西皐雜詠十七詠)', and 'Sameun-jeong 12 Gyoung(三隱亭十二景)' which are set on both villa, the intend to expand the garden area is strongly shown by the natural forest directing. As a result, Seogo-jeongsa and Sameun-jeong, located at Toero-li Miryang, are sharing the traditional Joseon dynasty era's custom, such as space and visual composition; however, it is different and attractive garden remains as a point of view of water directing, stone pagodas, and adopting foreign landscaping trees.

Anatomical Studies on Root Formation in Hypocotyl and Epicotyl Cuttings of Woody Plants (임목(林木)의 배축(胚軸) 및 유경삽수발근(幼茎揷穗発根)의 해부학적(解剖学的) 연구(研究))

  • Choi, Man Bong
    • Journal of Korean Society of Forest Science
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    • v.52 no.1
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    • pp.1-30
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    • 1981
  • The origin and development of adventitious roots was studied using hypocotyl and epicotyl cuttings of 34 species, 24 genus of woody plants. These cuttings obtained from young seedlings cultured in vials containing distilled water only. The several characteristics of cuttings materials studied are shown in Table 1. The results are summerized as follows: 1. The circumference shapes of cross-sections of hypocotyl and epicotyl cuttings can be divided into six categories, namely, round, irregular round, ellipse, irregular ellipse, square, and triangle. Species differences within a genus did not show any difference of hypocotyl and epicotyl cross-sections shape, however, a noticeable variation among genus or higher taxa. 2. The arrangements of vascular bundles in the cross-sections of hypocotyls or epicotyls were almost all collateral types and generally showed generic characteristics differing one to the other. However, there were some variations between species within the genus. Six models of vascular bundle arrangement were proposed for all the above speices. 3. The rooting portions of hypocotyl and epicotyl cuttings in this experimental materials can be grouped as follows: (1) Interfascicular parenchyma; (Thuja orientalis. T. orientalis for. sieboldii, Acer microsieboldianum, A. palmatum, A. saccharinum, Cercis chinensis, Lespedeza bicolor, Magnolia obovata, M. sieboldii, Mallotus japonicus, Staphylea bumalda) (2) Cambial and phloem parenchyma: (Chamaecyparis obtusa, C. pisifera, Albizzia julibrissin, Buxus microphylla var. Koreana, Cereis chinensis, Euonymus japonica, Firmiana platanifolia, Lagerstroemia indica, Ligustrum salicinum, L. obtusifolium, Magnolia kobus, M. obovata, Mallotus japonicus, Morus alba, Poncirus trifoliata, Quercus myrsinaefolia, Rosa polyantha, Styrax japonica, Styrax obassia) (3) Primary ray tissues; (Euonymus japonica, Styrax japonica) (4) Leaf traces; (Quercus acutissima, Q. aliena) (5) Cortex parenchyma; (Ailanthus altissima) (6) Callus tissues; (Castanea crenata, Quercus aliena, Q. myrsinaefolia, Q. serrata) 4. As a general tendency throughout the species studied, in hypocotyl cuttings, the adventitious root primordia were originated from the interfascicular parenchyma tissue, however, leaf traces and callus tissues were contributed to the root primordia formation in epicotyl cuttings. The hypocotyl cuttings of Ailanthus altissima exhibited a special performance in the root primordia formation, this means that cortex parenchyma was participated to the origin tissue. And in Firmiana platanifolia, differening from the other most species, the root primordia were formed at the phloem parenchyma adjacent outwardly to xylem tissue of vascular bundle system as shown photo. 48. 5. All the easy-to, or difficult-to root species developed adventitious roots in vials filled with distilled water. In the difficult-to-root species, however, root formations seemed to be delayed because they almost all had selerenchyma or phloem fiber which gave some mechanical hindrance to protrusion of root primordia. On the other hand, in the easy-to-root species they seemed to form them more easily because they did not have the said tissues. The rooting portions between easy-to-root and difficult-to-root species have not clearly been distinguished, and they have multitudinous variations. 6. The species structured with the more vascular bundles in number compared with the less vascular bundles exhibited delayed rooting. In the cuttings preparation, the proximal end of cuttings was closer to root-to-stem transition region, the adventitious root formation showed easier. 7. A different case occured however with the mature stem cuttings, in both the needle-leaved and the broad-leaved species. In the hypocotyl cuttings, parenchymatous tissues sited near the vascular bundles become the most frequent root forming portions in general and relevant distinctions between both species were hardly recognizable. 8. In the epicotyl cuttings, root primordia originated mainly in leaf traces in connection with cambial and phloems or callus tissues itself. In the hypocotyl cuttings, interfascicular parenchyma was the most frequent portion of the root primordia formation. The portions of root primordia had more connection with vascular cambium system, as the tissues were continuing to be developed.

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