• 한국축산식품학회지
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• 제39권4호
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• pp.565-575
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• 2019

This study evaluated Campylobacter jejuni risk in ground meat products. The C. jejuni prevalence in ground meat products was investigated. To develop the predictive model, survival data of C. jejuni were collected at $4^{\circ}C-30^{\circ}C$ during storage, and the data were fitted using the Weibull model. In addition, the storage temperature and time of ground meat products were investigated during distribution. The consumption amount and frequency of ground meat products were investigated by interviewing 1,500 adults. The prevalence, temperature, time, and consumption data were analyzed by @RISK to generate probabilistic distributions. In 224 samples of ground meat products, there were no C. jejuni-contaminated samples. A scenario with a series of probabilistic distributions, a predictive model and a dose-response model was prepared to calculate the probability of illness, and it showed that the probability of foodborne illness caused by C. jejuni per person per day from ground meat products was $5.68{\times}10^{-10}$, which can be considered low risk.

• Journal of Microbiology and Biotechnology
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• 제11권6호
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• pp.934-939
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• 2001

The growth inhibition of Campylobacter jejuni ATCC 33291 in the presence of $1\%$ acetic acid at 4, 25, and $42^{\circ}C$, followed by $25^{\circ}C$ and $4^{\circ}C$, at pH 5.5 and pH 6.5, and by the addition of $1\%$ acetic acid aat 4, 25, and $42^{\circ}C$ were determined to be 22, 8.5, and 1.4 min, respectively, in an FBP-SBB medium. The D values of C. jejuni were increased by the addition of chicken and did not follow the linear relationship observed in the FBP-SBB media without chicken. When using distilled water instead of FBP-SBB in the model system, the death rate of C. jejuni was dramatically accelerated. The injured or low cell numbers that were impossible to enumerate using the plate count method, were detected by a polymerase chain reaction and enrichment culture procedure. These results suggested that acetic acid is reliable and effective as a disinfectant, however, it is necessary to take additional care at refrigeration temperatures due to the potential of injred cells during poultry processing.

• 한국임상수의학회지
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• 제23권1호
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• pp.41-49
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• 2006

In this study, antimicrobial activity of oriental herbal medicine extract (OHME) was tested for some organisms and the preventive effects of OHME for the colonization of Campylobacter jejuni on epithelium of small intestine were examined in Korean native broiler chickens fed a forage added 1.0% OHME. The isolated Campylobacter spp were biotyped, serotyped and the susceptiblility of isolates to antimicrobial agent were examined. The growth of Staphylococcus aureus was inhibited in 0.25% OHME. C. jejuni and C. coli were inhibited in 0.1% OHME, and Salmonella spp, Lactobacillus acidophilus and Escheichia coli 0157 were inhibited in 2.0% OHME. For the application of forage added 1.0% OHME in broiler chicken farm, the frequency of Campylobacter spp from feces, liver and spleen sample of chickens were examined during 2 weeks interval. The frequence of Campylobacter spp in feces from chickens fed assorted forage (control group) was increased from 25% in first week to 75% in seventh week. But the frequence of Campylobacter spp in feces sample from chickens 134 forage added OHME was slightly reduced from 25% in first week to 15% in seventh week. The frequence of Campylobacter spp in liver, and spleen was 13.7% and 10% respectively after seventh week in control group, but the Campylobacter spp was not isolated after fifth week in live and spleen from chickens fed forage added OHME. Isolated 56 strains of thermophilic Campyiobacter from Korean native chickens was classified as C. jejuni (76.7%), C. coli (214%) and C. laridis (1.6%). The majority of 43 isolates of C. jejuni was classified on biotype I (60.4%), II (30.2%). Most of 12 isolates of C.coli were biotype I (83.3%). Isolated 31 strains C. jejuni of showed 11 different serotype, and serotype 36 (18.6%), 17 (13.9%)were most frequent. Isolated 10 strains of C. coli showed 5 different serotypes and serotype 31 (33.3%) and 21 (25%) were relatively common. Isolated Campylobacter spp were highly susceptible to nalidixic acid, amikacin, gentamicin, colistin and chloramphenucol.

• 한국동물위생학회지
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• 제30권1호
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• pp.77-84
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• 2007

The present study was carried out to investigate the incidence of Campylobacter spp. from the chicken carcasses in slaughterhouse. A total of 9 strains were primarily isolated from enrichment culture and selective culture of the sample with candle and microaerophilic chamber method. Nine of Gram-negative, catalase-positive and oxidase-positive strains were further isolated by the determination of biochemical characteristics and finally identified as Campylobacter jejuni with HIP 400F and HIP l134R primers. Therefore, this PCR method proved to be useful as a routine diagnostic test for the Campylobacter detection and confirmation of C. jejuni and C. coli in naturally contaminated poultry samples.

• 한국동물위생학회지
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• 제36권1호
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• pp.57-60
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• 2013

This study was carried out to investigate the prevalence and antimicrobial resistance patterns of Campylobacter jejuni isolated from duck feces. In total, 112 (32.9%) isolates of C. jejuni were identified from 430 duck feces samples from September to December, 2010. All isolates were susceptible to telithromycin, whereas majority of the C. jejuni isolates were resistant to azithromycin (18.8%), ciprofloxacin (86.6%), erythromycin (0.9%), gentamicin (15.2%), tetracycline (80.4%), florfenicol (3.6%), nalidixic acid (87.5%), clindamycin (7.1%). As a result, appropriate protocols for antimicrobial agents and strategies to reduce antimicrobial resistance will be needed in the future.

• 한국식품과학회지
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• 제43권1호
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• pp.119-123
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• 2011

본 연구에서는 두 종류의 선택배지를 활용한 배지배양법과 realtime PCR의 C. jejuni 검출능력을 비교하였다. 소시지, 쇠고기 분쇄육, 무순에 C. jejuni를 접종하고 Hunt broth로 증균배양 하였으며, mCCD agar와 Preston agar에 배양액을 획선도말하여 미호기적으로 배양하였다. 동시에 증균배양액에서 1 mL을 채취하여 realtime PCR을 실시하였다. 실험결과, real-time PCR은 쇠고기 분쇄육과 소세지에서 두 가지 선택배지와 비교하여 동일한 검출력을 보였으나 무순에서는 훨씬 더 많은 양성을 검출하였다(p<0.05). 두 배지간의 비교에서는 Preston agar와 mCCD agar는 통계학적 유의차가 없는 민감도를 보였다(p>0.05). 결론적으로 real-time PCR은 표준검출법인 배지배양법과 비교하여 동등하거나 우수한 민감도를 지닌 신속검출기법인 것으로 사료되며, 배지배양법에 앞서 선별검사로 사용할 경우 시간, 비용, 노동력 절감에 있어서 매우 유효한 방법이 될 것으로 판단된다.

• 대한미생물학회지
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• 제17권1호
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• pp.43-47
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• 1982

C. fetus suhsp. jejuni has been reported to be an important enteric pathogen in many parts of the world. Although the infection has been reported in Korea, the incidence is not known. In this study the results of stool culture during the period of August 1981 to July 1982 at Yonsei Medical Center was analyzed and the following results were obtained. 1. C. fetus subsp. jejuni was isolated from 0.8% of stool specimens. The isolation rate was lower than that of salmonella(3.3%) and shigella(7.1%). The isolation was most frequent in June and from $\leq$15-year-old patients. 2. All of the isolates from the patients were susceptible to chloramphenicol and erythromycin. It was noteworthy that 4 isolates were resistant to all of the aminoglycosides, i.e., amikacin, gen tamicin, kanamycin and tobramycin. 3. We also isolated C. fetus subsp. jejuni from chicken. When the susceptibility of the isolates was compared to that of the isolates from human the former were less susceptible to erythromycin(34.1%) and tetracycline(38.6%).

• 한국임상수의학회지
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• 제32권2호
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• pp.154-157
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• 2015

개에서 설사를 일으키는 주요 원인이 되는 장내 병원균으로 Salmonella spp., Shigella spp., Campylobacter spp., Clostridium spp.가 있다. 이들 세균은 배양으로 검출이 어렵다. 본 실험에서는 Salmonella spp., C. coli, C. jejuni, 그리고 Cl. perfringens를 신속하고 민감하게 검출할 수 있는 방법을 고안하였다. 정상견 71마리와 설사증상이 있는 66마리에서 수집한 분변 시료에서 장내 병원균의 유병률을 알아보고자 하였다. 장내 병원균은 실시간 중합효소 연쇄 반응 분석을 이용하여 검출하였다. 설사변에서 Salmonella spp., C. coli, C. jejuni, Cl. Perfringens는 정상변보다 검출률이 높았다. 개발한 다중실시간 중합효소연쇄반응은 분변시료의 병원균 존재 및 양 또는 기타 고유 서열을 확인하는데 유용하였다.

• 한국식품위생안전성학회지
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• 제3권1호
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• pp.27-36
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• 1988

사람과 동물에 장염을 일으키는 원인균으로서 식품위생학적으로 새로이 문제시 되고 있는 Campylobacter jujuni의 독장에서의 오염원과 오염경로 등을 조사하기 위하여 닭의 분변, 냉각전·후의 계육, 냉각수 , 내장적출용칼을 실험대상으로 하여, 본 균을 분리 동정하고 , 분리균의 생물형, 혈청형등을 조사한 바 다음과 같은 결과를 얻었다. 1. 각 도계공정별로 분리한 82주의 Campylobacter jujuni 는 닭의 분변으로부터 34.4%, 냉각후 계육 55.0% 냉각수 60.0%, 내장적출용칼로부터 45.0%의 불리율을 나타내었다. 2. 분리한 Campylobacter jujuni 균주의 생물형은 biotype I이 78.1%, biotype II가 21.9%이었으며, biotype III와 IV에 속하는 균주는 없었다. 3. 분리균 82주의 Campylobacter jujuni에 대한 혈청형 검사에서 biotype I에속하는 것은 모두 혈청형이 KIO 37 이었으며, biotype II에 속하는 균주의 혈청형은 동정할 수 없었다.

• 한국식품영양학회지
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• 제25권2호
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• pp.291-298
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• 2012

본 연구에서는 신생아 질환과 사망 원인 중 첫 번째에 해당하는 설사의 주된 원인균인 Campylbactor jejuni에 대한 쇠비름 발효액의 항균 효과를 검증해 보고자 하였다. 민간요법과 한방에서 널리 이용되어온 쇠비름은 각종 항균 효과나 항염증, 항암 효과 등이 있는 것으로 알려져 있는 식용 식물로, 여기에서 식물성 유산균을 분리해 각종 프로바이오틱스와 더불어 쇠비름 발효액을 제조하였다. Lactobacillus rhamnosus, L. acidophilus, L. bulgaricus, L. delbrueckii, L. plantarum, Leuconostoc mesenteroides 및 Bifidobacterium longum과 쇠비름에서 분리한 균을 쇠비름 추출물에 적용하였을 때, Leuconostoc mesenteroides와 쇠비름에서 분리한 식물성 유래 유산균이 쇠비름 추출액에서 가장 잘 자랐고, 여기에 2% yeast extract, 1% peptone 및 0.05~1% 인산을 첨가하였을 때 가장 적합한 발효액을 만들 수 있었다. 쇠비름 발효액에 들어있는 생균수는 발효 48시간 후 $1{\times}10^{12}\;CFU/m{\ell}$로 증가하였고, 2주간 냉장 보관을 한 이후에도 생균수가 $1.3{\times}10^{10}\;CFU/m{\ell}$로 남아있었다. 쇠비름 발효액의 pH와 산도는 발효 48시간 이후 3.7과 3.14로 각각 나타나 일반 유제품 발효액의 기준 범위 안에 들어감을 알 수 있었다. PLAB과 Leuconostoc mesenteroides로 만든 쇠비름 발효액은 10 $mg/m{\ell}$ 이상의 농도에서 C. jejuni에 대해 증식 억제효과를 나타냈으며, 이 억제 효과는 48시간 동안 지속되었다.