• 한방안이비인후피부과학회지
• /
• 제15권1호
• /
• pp.96-126
• /
• 2002

In the course of screening natural extracts for hair growth, we found that the extract of dried root of Sophora flavescens has the prominent hair growth promoting effect. After topical application of Sophora flavescens extract to the back of C57BL/6 mice, the earlier conversion of telogen-to-anagen phase was induced. In addition, the Sophora flavescens extract revealed to possess potent inhibitory effect on $5{\alpha}$-reductase Ⅰ and Ⅱ activity. The growth of dermal papilla cells and mouse vibrissae hair follicle cultured in vitro, however, was not affected by Sophora flavescens extract treatment. RT-PCR analysis showed that Sophora flavescens extract induced mRNA levels of growth factors such as insulin-like growth factor-Ⅰ and keratinocyte growth factor in dermal papilla cells, suggesting hair growth promoting effect of Sophora flavescens extract is mediated through inhibition of $5{\alpha}$-reductase type Ⅱ activity and the regulation of growth factors in dermal papilla cells. Furthermore, Sophora flavescens extract also showed anti-bacterial effect on Propionibacterium acnes. These results suggest that Sophora flavescens can be used as a potent treatment agent for helping hair growth stimulation and acne inhibition.

• 한방안이비인후피부과학회지
• /
• 제17권1호
• /
• pp.104-125
• /
• 2004

This study was carried out to investigate the anti-microbial of various herbal extracts on six types of bacteria related to skin diseases and effects of Saussurea lappa extract on dermatitis and acne. The results are followed ; 1. In the experiment on P. acnes using paper disk methods, Eugenia caryophyllata extract made 19mm clear zone, Saussurea lappa extract and Morus alba extract made 13mm and MIC of Morus alba extract and Eugenia caryophyllata extract was below 0.01$\%$. 2. In the experiment on S. aureus, extract of Eugenia caryophyllata, Poncirus trifoliata and Rubus coreanus made 9-10mm clear zone. 3. In the experiment on C. xerosis, Sesamum indicum extract made 16mm clear zone and MIC of that was 0.3$\%$. 4. In the experiment on C. albicans, extract of Cinnamomum cassia, Eugenia caryophyllata and Asparagus cochinchinensis made 9-10mm clear zone. 5. In the experiment on P. ovale, Cinnamomum cassia extract made 25mm clear one, MIC of that was 0.05$\%$. 6. In the experiment on T. mentagrophytes, Cinnamomum cassia extract made 26mm clear zone, extract of Eugenia caryophyllata, Rhizoma kaempferiae, Piper longum, Saussurea lappa and Zingiber officinale made 18-22mm clear zone, MIC of all extracts was below 0.02$\%$. 7. Saussurea lappa extract inhibited 85$\%$ and 28$\%$ of NO production at 50㎍／㎖ and 5 ㎍／㎖ 8. Saussurea lappa extract inhibited activity of COX Ⅱ over 50$\%$ at 10㎍／㎖ 9. Saussurea lappa extract had not radical scavenging activity and Saussurea lappa extract didn't inhibit manifestation of IL-1${\beta}$, IL-6 and TNF-${\alpha}$, and activity of 50- reductase I.

• 한국의류학회지
• /
• 제26권3_4호
• /
• pp.464-472
• /
• 2002

The aim of this study was to elucidate dyeability and antimicrobial activity of cotton fabrics dyed with Sophora Radix extracts according to various mordants. Dyes were extracted from Sophora Radix using ethanol. Then, cotton fabrics were dyed with extracts two times by post-mordanting method in which the extract was 60% (owf, the mordant was 3% (owf), L.R was 1:20, the temperature was 60~7$0^{\circ}C$, the time of dyeing was 60min., and the time of mordanting was 60min.. The dyeability was evaluated by surface color and color fastness. The skin microorganism was evaluated on S. sureus, B. subtilis, S. epidermidis, P. acnes, P. aeruginosa, E. coli, A. niger, C. albicans and T. mentagrophytes. The results are as follows; 1. When mordants were treated, surface color was 3.3Y to 0.1 GY in H (hue) value which indicated greenish yellow to yellow 2. The color fastness to perspiration, dry-cleaning, rubbing, and washing stain fabric showed 4~5 degree. The color fastness to light was improved to 4 degree by treatment of mordants. The color fastness to washing was 2 degree which was somewhat poor. 3. Cotton dyed with ethanol extracts was excellent on S. aureus, B. subtilis, S. epidermidis and p. antis. But that showed poor antibacterial activities on P. aeruginosa and E. coli such as gram negative baterials 4. Antibacterial activity of cotton fabrics dyed didn't be improved by treatment of mordant 5. Antifungal activity of cotton dyed with ethanol extracts was excellent on T. mentagrophytes. Especially, on T. mentagrophytes there was no growth of fungus during 72 hours in cotton dyed mordanting with SnCl$_2$.$_2$$H_2O$.

• 문화기술의 융합
• /
• 제8권6호
• /
• pp.605-614
• /
• 2022

본 연구에서는 오레가노를 초임계유체추출법과 열수추출법으로 추출하여 항균, 항산화 in vitro 실험을 수행하였다. 항균실험인 Disc diffusion method 결과, 초임계추출물에 대해서만 clear zone이 나타났다. S. aureus에 대한MIC는 초임계추출물에서만 관찰되었으며, 1000 ㎍/mL로 확인되었다. 열수추출물은 C. acnes에 대한 MIC가 125 ㎍/mL로 여드름균에 대한 항균력이 뛰어났다. 나아가 우리는 Biofilm inhibition assay를 통해 오레가노 초임계추출물이 125 ㎍/mL의 저농도에서도 S. aureus의 바이오필름을 70%이상 억제한다는 것을 발견하였다. DPPH radical 소거능, ABTS+ radical 소거능, SOD 유사활성능과 총 폴리페놀 함량 측정에서 열수추출물의 항산화력이 초임계추출물보다 우수하게 측정되었다. 나아가, 앞서 확인된 오레가노 초임계추출물의 S. aureus 바이오필름 억제능을 활용하여 아토피 피부에 적합한 소재를 개발하고자 하였다. 오레가노 초임계추출물의 낮은 용해도를 극복하고 안정성을 증가시키기 위하여 리포좀을 이용하였다.

• Journal of Applied Biological Chemistry
• /
• 제53권3호
• /
• pp.162-169
• /
• 2010

도화 추출물의 항산화능 및 항염증 효과를 검증하여 기능성 화장품 소재로서의 가능성을 검증하였다. 도화의 항산화능 측정으로 전자공여능을 측정한 결과 도화 에탄올 및 아세톤 추출물 500ppm에서 90.0% 이상의 효과를 나타내어, 합성 항산화제로 알려진 BHT와 유사한 효과를 나타내었으며, SOD 유사활성능 측정 결과 도화 에탄올 및 아세톤 추출물 1,000ppm에서 40.0% 이상의 활성을 나타내었다. Xanthine oxidase 저해활성능 측정 결과 도화 에탄올 및 아세톤 추출물 1,000 ppm에서 vitamin C와 유사한 30.0%의 효과를 나타내어, 도화 열수 추출물에 비해 도화 에탄올 및 아세톤 추출물의 효과가 높게 나타났다. 항염증 효과 측정으로 hyaluronidase 저해활성을 측정한 결과 도화 열수, 에탄올 및 아세톤 추출물에서 35.0%의 저해활성을 나타내었으며, macrophage 세포를 이용한 nitric oxide (NO) 저해활성을 측정한 결과 도화 추출물 모든 농도에서 12시간 이후부터 NO 생성량이 억제됨을 확인할 수 있었다. 또한 피부염증 유발균인 Staphylococcus epidermidis와 Propionibacterium acnes의 항균효과를 측정한 결과 도화 에탄올 추출물에서 항균 효과를 나타내었다.

• Journal of Microbiology and Biotechnology
• /
• 제28권5호
• /
• pp.739-747
• /
• 2018

The low solubility and high-cost recovery of Propionibacterium acnes polyunsaturated fatty acid isomerase (PAI) are key problems in the bioproduction of high value-added conjugated linoleic acid (CLA). To improve the solubility of recombinant PAI, six chaperone proteins were coexpressed with PAI. Introduction of GroELS proteins dramatically improved the PAI solubility from 29% to 97%, with increased activity by 57.8%. Combined expression of DnaKJ-GrpE and GroELS proteins increased the activity by 11.9%. In contrast, coexpression of DnaKJ-GrpE proteins significantly reduced the activity by 57.4%. Plasmids pTf16 harboring the tig gene and pG-Tf2 containing the tig and groEL-groES genes had no visible impact on PAI expression. The lytic protein E was then introduced into the recombinant Escherichia coli to develop a cell autolysis system. A 35% activity of total intracellular PAI was released from the cytoplasm by suspending the lysed cells in distilled water. The PAI recovery was further improved to 81% by optimizing the release conditions. The lipase from Rhizopus oryzae was also expressed in E. coli, with an extracellular activity of 110.9 U/ml. By using the free PAI and lipase as catalysts, a joint system was established for producing CLA from sunflower oil. Under the optimized conditions, the maximum titer of t-10,c-12-CLA reached 9.4 g/l. This work provides an effective and low-cost strategy to improve the solubility and recovery of the recombinant intracellular PAI for further large-scale production of CLA.

• 한방안이비인후피부과학회지
• /
• 제15권2호
• /
• pp.53-79
• /
• 2002

To screen the effective materials for hair loss treatment, several natural extracts were tested using in vivo and in vitro test models. Firstly, all test materials were applicated onto the back skin of C57BL／6 mouse and then hair growth promoting effect was measured using hair growth index. As a result, Prunus mume, black bean, Brassica campestris subsp. black sesame and Rubi Fructus showed potent hair growth promoting effect, ranking as 1.5-2.0 of hair growth index. However, there were no plant extracts, which have remarkable potential of growth promotion of human hair dermal papilla cells cultured in vitro. In the experiments of 5${\alpha}$-reductase type Ⅱ inhibition assay, Prunus mume, Eriobotryae Folium showed effective potential to inhibit the activity of 5${\alpha}$-reductase type Ⅱ. To investigate the possible involvement of the effect of several plant extracts on the gene expression of growth factors in human hair dermal papilla cells, RT - PCR analyses were performed. However, there were no plant extracts, which have profound effect on the gene expression of several growth factors such as IGF-I, KGF, HGF and VEGF in the dermal papilla cells. Another tests for inhibition of microbial such as P. acne were also carried out to find whether these plant extracts have anti -microbial activities. Rubi Fructus showed anti -microbial effects on Propionibacterium acnes, which is believed as a pathogen of acne. Together, these results showed several plant extracts can be used for hair growth promotion.

• 한방안이비인후피부과학회지
• /
• 제15권2호
• /
• pp.80-103
• /
• 2002

To screen the effective materials for hair loss treatment, several natural extracts were tested using in vivo and in vitro test models. Firstly, all test materials were applicated onto the back skin of C57BL／6 mouse and then hair growth pormoting effect were measured using hair growth index As a result, Polygonum muitifiorum Thunb and Terrninalia chebula Retz. showed potent hair growth promoting effect, ranking as 1.5-2.0 of hair growth index. However, there were no plant extracts, which have remarkable potential of growth promotion of human hair dermal papilla cells cultured in vitro. In the experiments of 5${\alpha}$-reductase type Ⅱ inhibition assay, Morus alba L., Chaenomelis Fructus, Saussureae Radix, Angelicae Gigantis Radix, Polygonum multifiorum Thunb, and Angelica dahurica (Fischer) Bentham et Hooker f. showed effective potential to inhibit the activity of 5${\alpha}$-reductase type Ⅱ. To investigate the possible involvement of effects of several plant extracts on the gene expression of growth factors in human hair dermal papilla cells, RT-PCR analyses were performed. As a consequences, Mentha haplocalyx Briq., Cimicifuga foetida L., Eclipta prostrata (L.) L., Pinus densiflora S. et. Z, and Polygonum muitifiorum Thunb revealed the regulatory roles on the expression of growth factors such as IGF-I, KGF, HGF and VEGF in the dermal papilla cells. Another test for inhibition of microbial such as P. acne and P. ovale were also carried out to find whether these plant extracts have anti-microbial activities. Morus alba L. and Chaenomelis Fructus showed anti-microbial effects on Propionibacterium acnes, which is believed as a pathogen of acne. Together, these results showed several plant extracts can be used for hair growth promotion.

• 대한화장품학회지
• /
• 제41권4호
• /
• pp.361-373
• /
• 2015

본 연구는 한국 제천에서 재배된 감초(Glycyrrhiza uralensis Fisher, G. uralensis)를 대상으로 추출 조건(85% 에탄올, 추출 온도 및 시간)별로 추출한 추출물에 대하여 항산화 활성 및 항균 활성을 측정하고 이로부터 최적의 추출 조건을 선정하여 감초 추출물을 화장품 원료로 개발하기 위한 제조 공정에 활용하고자 하였다. 실험에 사용한 시료는, 각 추출 조건에서 얻어진 추출물을 농축하여 분말로 한 시료(시료-1)와 추출물을 농축하지 않고 추출물 원액을 그대로 사용한 시료(시료-2)이다. 항산화 활성은 라디칼 소거활성, 총항산화능, 활성 산소로 유도된 세포손상에서 세포보호효과를 측정하였다. 항균 활성은 피부 상재균에 대한 최소저해농도를 측정하였다. 1,1,-Diphenyl-2-picrylhydrazyl를 이용한 라디칼 소거활성에서, 시료-1은 $100{\mu}g/mL$에서 추출시간이 6 h일 때가 12 h일 때보다 약 10% 정도 더 큰 라디칼 소거활성을 나타냈다. 반면에 시료-2의 라디칼 소거활성은 추출 시간에 따른 유의적 차이를 나타내지 않았다. 한편 동일 온도에서 12 h 추출한 추출물의 수율은 6 h 추출한 경우의 수율보다 2.6배 더 컸다. 하지만 총 플라보노이드 함량은 1.1배 정도 더 크게 나타났다. 따라서 추출 시간이 길어도 총플라보노이드 함량은 거의 증가하지 않았음을 보여준다. 추출 조건별 감초 추출물의 라디칼 소거활성, 총항산화능 및 세포보호효과가 추출물의 수율을 반영한 것이 아니라 추출물 중에 함유된 총플라보노이드 함량에 의존함을 나타내고 있다. 피부 상재균에 대한 항균 활성은 추출 조건별 동일 농도(시료-1)에서 측정했을 때, 세 균주(S. aureus, B. subtilis, P. acnes)에 대해 25 및 $40^{\circ}C$에서 추출된 감초추출물($156{\mu}g/mL$)은 methyl paraben ($2,500{\mu}g/mL$)보다 약 16배 정도로 매우 큰 항균 활성을 나타냈다. 결론적으로 항산화 활성 및 항균 활성이 큰 감초 추출물의 최적 추출 조건은 85% 에탄올로 $40^{\circ}C$에서 6 h 추출한 추출물이 최적임을 확인할 수 있었다. 본 연구 결과, 화장품에 항산화 활성 및 항균 활성이 큰 감초 추출물을 원료화하기 위해서는 추출 조건별 추출물의 수율, 활성 성분의 수율, 추출물의 농도별 활성 평가와 추출 수율이 반영된 추출물 원액 자체의 활성을 종합 평가해서 추출 조건을 선정해서 원료의 제조 공정에 반영하는 것이 중요함을 시사한다.

• 대한화장품학회지
• /
• 제32권1호
• /
• pp.53-58
• /
• 2006

본 연구는 우리나라의 야산이나 길가, 들판 등에 흔히 자라는 여러해살이 풀이며, 오래 전부터 민간요법 및 한방에 유용 한 약재로 사용되어 지고 있는 선학초(Agrimonia pilosa Ledeb.)를 이용하여 여드름 유발균에 대한 항균활성을 평가하고, 이를 토대로 항여드름 관련 화장품 소재를 개발하는데 그 목적이 있다. 선학초를 methanol로 추출한 후, 이 추출물을 여드름 유발균주인 P. acne를 이용하여 minimum inhibitory concentration (MIC) 및 paper disk diffusion assay로 항균 효과를 측정하였으며, 추출물에 대한 온도 및 pH 변화에 따른 안정성을 평가하였다. 추출물을 이용하여 P. acne에 대한 MIC 값은 0.005 mg/mL로 측정되었으며, 이는 대조군으로 사용한 항생제인 triclosan의 MIC 값(0.004 mg/mL)과 비교했을 때 시료가 선학초 전초에 대한 crude extract임을 고려하면 매우 항균활성이 높은 것으로 평가되었다. 추출물에 대한 paper disk diffusion assay를 수행한 결과, 대조군으로 사용한 triclosan 처럼 높은 활성을 나타내는 것으로 측정되었으며, Staphylococcus aureus를 비롯한 여러 종의 호기성 균주에도 선학초추출물은 항균활성이 우수한 것으로 평가되었다. 선학초 추출물의 온도, pH 안정성을 평가한 결과, 70, 80, 90, 100, $121^{\circ}C$ 등 다양한 온도 조건과 $pH 2{\sim}pH 11$의 변화에 있어서도 그 효능이 변하지 않고 안정된 상태로 유지되는 것으로 관찰되었다.