• Korean Journal of Microbiology
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• v.10 no.1
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• pp.1-8
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• 1972

This experiment was carried out to investigate the physiological characteristics of isolated bacteria, Ferrobacillus ferooxidans from copper mine water in Korea. The results obtained were as follows ; 1. The optimum pH range for the growth of these bacteria was 2.0-3.0 and optimum temperature was $20^{\circ}C$-$30^{\circ}C$. 2. The oxidation curves of ferrous iron to the ferric iron ran parallel with the growth curves. 3. The optimum nitrogen concentration was 400-800 ppm and the minimal flow rate of air for the maximal growth of the bactria was 70 ml air/min./200ml medium. 4. The growth of these bacteria was inhibited by the absence of ferrous iron and by the addition of sulfur. 5. Ferrous iron at a concentration of 9000 ppm, appeared to be optimum for the most rapid growth of Ferrobacillus ferrooxidans.

• Journal of the Korean Magnetic Resonance Society
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• v.19 no.1
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• pp.29-35
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• 2015

The microscopic dynamics of $CsH_2PO_4$, with two distinct hydrogen bond lengths, are studied by static nuclear magnetic resonance (NMR) and magic angle spinning (MAS) NMR. The proton dynamics of the two crystallographically inequivalent hydrogen sites were discussed in terms of the $^1H$ NMR and $^1H$ MAS NMR spectra. Although the hydrogen bonds have two inequivalent sites, H(1) and H(2), distinct proton dynamics for the two sites were not found. Further, the $^{133}Cs$ spectrum is more or less continuous near $T_{C1}$ (=153 K). Finally, the phase transition mechanism of $T_{C1}$ in $CsH_2PO_4$ is related to the ordering of protons.

• Journal of the Korean Magnetic Resonance Society
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• v.21 no.2
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• pp.63-66
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• 2017

The $^{14}N$ NMR spectra for $(NH_4)_2SO_4$ crystals were obtained near the phase transition temperature $T_C=223K$, and were found to precisely reflect the symmetry change in the crystal at this first-order phase transition. Changes in the resonance frequencies near $T_C$ were attributed to the structural phase transition. In the ferroelectric and paraelectric phases, two inequivalent NH4 groups were distinguished in the $^{14}N$ NMR spectra. The two types, $NH_4$(1) and $NH_4$(2), have slightly different local environments. Consequently, we conclude that the phase transition is caused by the change in the environment of the $^{14}N$ nuclei in the $NH_4$ groups, rather than by the $SO_4$ groups.

• BMB Reports
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• v.56 no.4
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• pp.225-233
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• 2023

Hepatocellular carcinoma (HCC) is a very common form of cancer worldwide and is often fatal. Although the histopathology of HCC is characterized by metabolic pathophysiology, fibrosis, and cirrhosis, the focus of treatment has been on eliminating HCC. Recently, three-dimensional (3D) multicellular hepatic spheroid (MCHS) models have provided a) new therapeutic strategies for progressive fibrotic liver diseases, such as antifibrotic and anti-inflammatory drugs, b) molecular targets, and c) treatments for metabolic dysregulation. MCHS models provide a potent anti-cancer tool because they can mimic a) tumor complexity and heterogeneity, b) the 3D context of tumor cells, and c) the gradients of physiological parameters that are characteristic of tumors in vivo. However, the information provided by an multicelluar tumor spheroid (MCTS) model must always be considered in the context of tumors in vivo. This mini-review summarizes what is known about tumor HCC heterogeneity and complexity and the advances provided by MCHS models for innovations in drug development to combat liver diseases.

• Horticultural Science & Technology
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• v.17 no.6
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• pp.755-757
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• 1999

This study was conducted to investigate the optimum temperature after BA treatments for the indution of flowering in Cymbidium ensifolium 'Tekkotsusosin'. Flowering rate was 90% at $30/25^{\circ}C$ (day/night), 50% at $25/20^{\circ}C$ and the natural condition as control, and non flowering at $20/15^{\circ}C$. Malformation rate of flower shape was at $25/20^{\circ}C$, 4% at $30/25^{\circ}C$, and 9% in the natural condition. The length and number of flowering stalks per plant were most at $30/25^{\circ}C$ treatment. After BA spraying, the lowest air temperature for flowering was assumed to be about $20^{\circ}C$.

• Journal of the Korean Society of Clothing and Textiles
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• v.31 no.1 s.160
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• pp.119-130
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• 2007

The objects of this study were to investigate responses and peculiarity during local cooling by parts of the human body and to show permissible safety limits without injurious to his health because of excessive cooling when he works hot environments. It were measured rectal temperature, skin temperature, heart rate, total body weight loss, local sweat in back and thigh, clothing microclimate and subjective sensation on 8 subjects and cooling parts were head, neck, chest, abdomen, back, waist, hip, upper arm, forearm, hand, thigh, calf and foot. According to above-mentioned the first experiment, we chose permissible safety limits by parts of the human body for one hour. In the second experiment, it was showed permissible safety limits by parts which examined their safety about health through 4 hours cooling test on 3 subjects. The results are as follows: 1. As a result of the first experiment, we chose permissible safety limits by parts, as follows, head $25^{\circ}C$, neck $20^{\circ}C$, chest $27^{\circ}C$, abdomen $25^{\circ}C$, back $20^{\circ}C$, waist $20^{\circ}C$, upper arm $20^{\circ}C$, forearm $20^{\circ}C$, hand $23^{\circ}C$, thigh $20^{\circ}C$, calf $20^{\circ}C$ and foot $23^{\circ}C$ in $37^{\circ}C$, 50%R.H. environment for 1 hour. 2. As a result of the second experiment, cooling on these safety limits temperatures except chest didn't have a bad effect on health. So it was proved that right permissible safety limits of chest was $28^{\circ}C$. From these results, it has been suggested that skin temperature didn't fall below permissible safety limits when human body was to be cool by parts.

• Biomedical Science Letters
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• v.29 no.1
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• pp.11-25
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• 2023

In hepatocellular carcinoma (HCC), chromosome 4 open-reading frame 47 (C4orf47) has not been so far investigated for its prognostic value or association with infiltrating immune cells. We performed bioinformatics analysis on HCC data and analyzed the data using online databases such as TIMER, UALCAN, Kaplan-Meier plotter, LinkedOmics, and GEPIA2. We found that C4orf47 expression in HCC was higher compared to normal tissues. High C4orf47 expression was associated with a worse prognosis in HCC. The correlation between C4orf47 and infiltrating immune cells is positively associated with CD4+T cells, B cells, neutrophils, macrophages, and dendritic cells in HCC. Moreover, high C4orf47 expression was correlated with a poor prognosis of infiltrating immune cells. Analysis of C4orf47 gene co-expression networks revealed that 12501 genes were positively correlated with C4orf47, whereas 7200 genes were negatively correlated. The positively related genes of C4orf47 are associated with a high hazard ratio in different types of cancer, including HCC. Regarding the biological functions of C4orf47 gene, it mainly regulates RNA metabolic process, DNA replication, and cell cycle. The C4orf47 gene may play a prognostic role by regulating the global transcriptome process in HCC. Our findings demonstrate that high C4orf47 expression correlates with poor prognosis and tumor-infiltrating immune cells in HCC. We suggest that C4orf47 is a novel prognostic biomarker and potential immune therapeutic target for HCC.

• BMB Reports
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• v.38 no.1
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• pp.28-33
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• 2005

A gene coding a novel isoform of carbamyl phosphate synthetase I (CPS1) was cloned from a human testicular library. As shown by cDNA microarray hybridization, this gene was expressed at a higher level in human adult testes than in fetal testes. The full length of its cDNA was 3831 bp, with a 3149 bp open reading frame, encoding a 1050-amino-acid protein. The cDNA sequence was deposited in the GenBank (AY317138). Sequence analysis showed that it was homologous to the human CPS1 gene. The putative protein contained functional domains composing the intact large subunit of carbamoyl phosphate synthetase, thus indicated it has the capability of arginine biosynthesis. A multiple tissue expression profile showed high expression of this gene in human testis, suggesting the novel alternative splicing form of CPS1 may be correlated with human spermatogenesis.

• Journal of Microbiology and Biotechnology
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• v.20 no.3
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• pp.513-517
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• 2010

A thermostable trehalose synthase (TtTSase) from Thermus thermophilus HJ6 was immobilized on chitosan activated with glutaraldehyde. The yield of immobilization was evaluated as 39.68%. The optimum pH of the immobilized enzyme was similar to that of the free enzyme. However, the optimal temperature ranges were shifted by about $4^{\circ}C$ owing to better thermal stability after immobilization. The half-life of heat inactivation for free and immobilized enzymes was 5.7 and 6.3 days at $70^{\circ}C$, respectively, thus showing a lager thermostability of the immobilized enzyme. When tested in batch reaction, the immobilized enzyme retained its relative activity of 53% after 30 reuses of reaction within 12 days, and still retained 82% of its initial activity even after 150 days at $4^{\circ}C$. A packed-bed bioreactor with immobilized enzyme showed a maximum yield of 56% trehalose from 100 mM maltose in a continuous recycling system (bed volume: 10 ml) under conditions of pH 7.0 and $70^{\circ}C$.

• Journal of the Korean Society for Nondestructive Testing
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• v.25 no.1
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• pp.9-14
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• 2005

The angular dependence(profile) of backward radiated ultrasound was measured for glass specimens with random surface roughness using ultrasonic goniometer that ran changes the incident angle continuously. It was concluded that the roughened region had greater acoustic impedance than the unperturbed region. The comparison of backward radiations showed that the amplitude of peak and the area of radiation profile were increased with surface roughness. It was suggested from the sensitive dependence of the profile area that the profile of backward radiation could be applied to in the nondestructive evaluation of sulfate region. Inclined C-scan technique with the transducer inclined at Rayleigh angle showed the reverse of luminosity and the high signal to noise ratio so that it provided high resolution.