• Title/Summary/Keyword: C-26

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Molecular Discrimination of Dinoflagellates Cochlodinium Polykrikoides Margalef, Gyrodinium Impudicum Fraga et Bravo and Gymnodinium Catenatum Graham using RAPD-PCR Method (RAPD-PCR 방법을 이용한 Cochlodinium polykrikoides Gyrodinium impudicum, Gymnodinium catenatum의 분자생물학적 진단)

  • Cho, Eun-Seob
    • Journal of Life Science
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    • v.13 no.5
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    • pp.651-657
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    • 2003
  • Randomly amplified polymorphic DNA (RAPD) analysis was used to study genetic relationships among C. polykrikoides, G. impudicum and G. catenatum, which possess similar morphological features. Four of 12 primers were selected and 59 amplification products ranged from 0.2 kb to 3.0 kb. The number of polymorphic products in C. polykrikoides, G. impudicum and G. catenatum was 16 (27.1%), 8 (13.5%), and 16 (27.1%), respectively, while 17 were monomorphic. Number of species-specific bounds was 26 (44.0%), 34 (57.6%), 26 (44.0%) in C. polykrikoides, G. impudicum and G. catenatum, respectively. The genetic similarity between C. polykrikoides and G. impudicum/G. catenatum was 0.83, whereas G. impudicum and G. catenatum was 0.78. Our results suggest that C. polykrikoides, G. impudicum and G. catenatum are extremely different on the basis of RAPD analysis, despite similarity based on their morphology. The RAPD technique appears to be efficient in detecting genetic variation in these dinoflagellates.

Effect of Retrograde Restraint of Rice Cake Using Raw Starch Saccharifying ${\beta}-amylase$ from Bacillus polymyxa No. 26 (Bacillus polymyxa No. 26의 생전분 당화형 ${\beta}-amylase$를 이용한 떡의 노화억제효과)

  • Sohn, Cheon-Bae;Lee, Sang-Mee
    • Korean Journal of Food Science and Technology
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    • v.26 no.4
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    • pp.459-463
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    • 1994
  • To improve the quality of rice-cake(Cholpyon), raw starch saccharifying (${\beta}-amylase$ from Bacillus polymyxa No. 26 was used in process of raw rice-cake production. 30g of raw rice flour was incubated with $0{\sim}1,200$ RS units of the enzyme for 5 hr at $45^{\circ}C$, and then steamed and stored for 40 hr at $4^{\circ}C$. In instrumental analysis, control group, which was incubated without addition of (${\beta}-amylase$, was completely hardened after incubation for $12{\sim}24$ hr at $4^{\circ}C$. In contrast, enzyme-treated group was not retrograded, and showed a great differences in hardness, cohesiveness and chewiness. On the other hand, in sensory analysis, the effect of the enzyme treatment was higher values of hardness, moistness, and sweetness than these of control group. Therefore, these results clearly suggested that ${\beta}-amylase$ was fully active to degrade raw rice starch in process of rice-cake production, resulting in improvement of starch retrogradation, good digestibility, and taste.

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Site Selection for Growing "Fuji" Apple in Jincheon County Using Climatology (진천군의 수치기후도와 후지품종 재배적지 탐색)

  • 이강일;서형호;서희철;정유란
    • Proceedings of The Korean Society of Agricultural and Forest Meteorology Conference
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    • 2003.09a
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    • pp.23-26
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    • 2003
  • 월별 최고기온 및 최저기온 분포도를 작성하였다. 그림은 4월의 기온으로서, 기온의 변이폭은 최고기온이 12 ~ 2$0^{\circ}C$, 최저기온이 -2 ~ 8$^{\circ}C$ 이다. 사과원 B 주변은 최고기온 18$^{\circ}C$, 최저기온 2$^{\circ}C$로 추정된다. 후지품종의 개화는 남서쪽에서 4월 26일 처음 시작되어 서부지역이 4월말, 동부지역이 5월 초에 만개 한다.(중략)

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External Condensation Heat Transfer Coefficients of R245fa on Low Fin and Turbo-C Tubes (낮은 핀관과 Turbo-C 촉진관에서 R245fa의 외부 응축 열전달계수)

  • Shim, Yun-Bo;Park, Ki-Jung;Jung, Dong-Soo
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.21 no.3
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    • pp.167-175
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    • 2009
  • In this study, condensation heat transfer coefficients(HTCs) of R22, R123, R134a and R245fa are measured on both 26fpi low fin and Turbo-C tubes. All data are taken at the vapor temperature of $39^{\circ}C$ with a wall subcooling of $3{\sim}8^{\circ}C$. Test results show that HTCs of the newly developed low vapor pressure alternative refrigerant, R245fa, are $7.8{\sim}9.2%$ and $10.3{\sim}18.6%$ higher than those of R123 for 26fpi low fin tube and Turbo-C tube respectively. For all refrigerants tested, HTCs of Turbo-C enhanced tube are higher than those of 26fpi low fin tube. For the low fin tube, Beatty and Katz's prediction equation yielded 20% deviation for all fluids. The heat transfer enhancement ratio of R245fa on the Turbo-C tube is $5.9{\sim}6.4$ while that of R123 is $5.7{\sim}5.9$. From the view point of environmental safety and condensation heat transfer, R245fa is a long term candidate to replace R123 currently used in centrifugal chillers.

Antitumor Activity of the Korean Mistletoe Lectin is Attributed to Activation of Macrophages and NK Cells

  • Yoon, Tae-Joon;Yoo, Yung-Choon;Kang, Tae-Bong;Song, Seong-Kyu;Lee, Kyung-Bok;Her, Erk;Song, Kyung-Sik;Kim, Jong-Bae
    • Archives of Pharmacal Research
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    • v.26 no.10
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    • pp.861-867
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    • 2003
  • Inhibitory effect of the lectins (KML-C) isolated from Korean mistletoe (KM; Viscum album coloratum) on tumor metastases produced by murine tumor cells (B16-BL6 melanoma, colon 26M3.1 carcinoma and L5178Y-ML25 lymphoma cells) was investigated in syngeneic mice. An intravenous (i.v.) administration of KML-C (20-50 ng/mouse) 2 days before tumor inoculation significantly inhibited lung metastases of both B16-BL6 and colon 26-M3.1 cells. The prophylactic effect of 50 ng/mouse of KML-C on lung metastasis was almost the same with that of 100 $\mu$ g/mouse of KM. Treatment with KML-C 1 day after tumor inoculation induced a significant inhibition of not only the experimental lung metastasis induced by B16-BL6 and colon 26M3.1 cells but also the liver and spleen metastasis of L5178Y-ML25 cells. Furthermore, multiple administration of KML-C given at 3 day-intervals after tumor inoculation led to a significant reduction of lung metastasis and suppression of the growth of B16-BL6 melanoma cells in a spontaneous metastasis model. In an assay for natural killer (NK) cell activity. i.v. administration of KML-C (50 ng/mouse) significantly augmented NK cytotoxicity against Yac-1 tumor cells 2 days after KML-C treatment. In addition, treatment with KML-C (50 ng/mouse) induced tumoricidal activity of peritoneal macrophages against B16-BL6 and 3LL cells. These results suggest that KML-C has an immunomodulating activity to enhance the host defense system against tumors, and that its prophylactic and therapeutic effect on tumor metastasis is associated with the activation of NK cells and macrophages.

Taxonomic re-examination of a carpet-like Codium (Chlorophyta) from Jeju, Korea

  • Lee, Hyung-Woo;Kim, Myung-Sook
    • ALGAE
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    • v.26 no.3
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    • pp.221-227
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    • 2011
  • There are three carpet-like Codium species with a broadly expanded thallus reported from Korea and Japan: C. latum Suringar, C. tapetum Y. Lee, and C. tenuifolium Shimada, Tadano and J. Tanaka. During surveys of green algal diversity, we encountered a Codium species with carpet-like thalli from several sites on Jeju Island. To confirm the taxonomic identity of these specimens, we studied the morphological features and obtained rbcL gene sequences. The morphological and sequence data indicated that the carpet-like Codium specimens from Jeju are C. tenuifolium from Japan, but distant from Japanese C. latum. We propose that the carpet-like specimens from Jeju should be renamed C. tenuifolium, instead of C. tapetum or C. latum. C. tenuifolium is characterized by a tenuous, carpet-like and erect thallus with a very short and slightly compressed stipe arising from a discal holdfast, sub-pyriform and clavate utricles with a tumid and swollen apical head, and globular or sub-globular gametangia issued at the basal portion of the utricles.

Mechanical Behavior of Al/C60-fullerenes Nanocomposites (풀러렌이 분산된 알루미늄기지 나노복합재의 기계적 거동)

  • Choi, Hyun-Joo
    • Composites Research
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    • v.26 no.2
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    • pp.111-115
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    • 2013
  • Aluminum-based composites containing $C_{60}$-fullerenes are produced by hot rolling of ball-milled powder. The grain size of aluminum is effectively reduced to ~100 nm during ball-milling processes, leading to grain refinement strengthening of the composite. Furthermore, $C_{60}$-fullerenes are gradually dispersed during ball-milling processes and hence the strength of the composite increases with the volume of $C_{60}$-fullerenes. The composite containing 10 vol% $C_{60}$-fullerenes with a grain size of ~ 100 nm exhibits ~1 GPa of compressive strength.

New Species of Genus Coeloplana (Ctenophora: Tentaculata: Platyctenida) from Korea

  • Song, Jun-Im;Hwang, Sung-Jin
    • Animal Systematics, Evolution and Diversity
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    • v.26 no.3
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    • pp.217-221
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    • 2010
  • Since November, 2006, the new creeping platyctenid ctenophore, Coeloplana anthostella n. sp., has been collected from twigs and polyp masses of encrusting dendronephthyans at a depth of 26-32 m off the coast of Munseom, Jejudo Island. This new species is distinguished from C. bocki Komai, 1920 and C. komai Utinomi, 1963 by its smaller size, the absence of dorsal papillae, and the deep scarlet colored star-shaped markings on the dorsal plane. This novel addition to ctenophore fauna is presently described and illustrated in detail.

Fixing Behaviors of Dimethylamino Anthraquinone Disperse Dyes and Monochlorotriazinyl Azo Reactive Dyes on P/C Blended Fabrics in One-Step Printing (디메틸아미노안트라퀴논계 분산염료와 아조계 모노클로로트리아진형 반응염료에 의한 P/C혼방직물의 일단계 날염에 있어 고착거동)

  • Park, Geon-Yong;Seo, Gi-Sung
    • Textile Coloration and Finishing
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    • v.19 no.3
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    • pp.18-25
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    • 2007
  • The fixing behaviors of anthraquinone disperse dyes containing dimethylamino substituent, such as C. I. Disperse Violet 26(D.V.26) and C. I. Disperse Blue 14(D.V.14), or containing diamino substituent, such as C. I. Disperse Blue 73(D.B.73), and monochlorotriazinyl azo reactive dyes, such as C. I. Reactive Orange 13(R.O.13), C. I. Reactive Red 3(R.R.3). C. I. Reactive Yellow 2(R.Y.2) on polyester/cotton blend(P/C) fabrics were examined for the one-step printing of P/C fabrics. The high temperature steaming of $175^{\circ}C$ is the most satisfactory fixing method for P/C one-step printing with above disperse and reactive dyes among the four different fixing methods: $175^{\circ}C$ steaming, $102^{\circ}C$ steaming${\rightarrow}175^{\circ}C$ steaming, $190^{\circ}C$ thermosol, $102^{\circ}C$ steaming${\rightarrow}190^{\circ}C$ thermosol. $190^{\circ}C$ thermosol is unfit to fix R.R.3 and R.Y.2 whose heat stability is poor. It was found that D.V.26 and D.B.14 containing dimethylamino substituent are unstable for heat and alkali, but D.B.73 is stable for them to print P/C blend fabrics with R.O.13 which is also stable for heat. Therefore we found that D.B.73, R.O.13 and a pair of D.B.73 and R.O.13 were very suitable for one-step printing of P/C blend fabrics.