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Antitumor Activity of the Korean Mistletoe Lectin is Attributed to Activation of Macrophages and NK Cells  

Yoon, Tae-Joon (Institute for Biomedical Research, Han Dong University)
Yoo, Yung-Choon (Department of Microbiology, College of Medicine, Konyang University)
Kang, Tae-Bong (Institute for Biomedical Research, Han Dong University)
Song, Seong-Kyu (Institute for Biomedical Research, Han Dong University)
Lee, Kyung-Bok (Department of Biochemistry, College of Medicine, Konyang University)
Her, Erk (Department of Immunology, College of Medicine, Kon-Kuk University)
Song, Kyung-Sik (School of Applied Biology and Chemistry, College of Agriculture and Life Science, Kyungpook National University)
Kim, Jong-Bae (Institute for Biomedical Research, Han Dong University)
Publication Information
Archives of Pharmacal Research / v.26, no.10, 2003 , pp. 861-867 More about this Journal
Abstract
Inhibitory effect of the lectins (KML-C) isolated from Korean mistletoe (KM; Viscum album coloratum) on tumor metastases produced by murine tumor cells (B16-BL6 melanoma, colon 26M3.1 carcinoma and L5178Y-ML25 lymphoma cells) was investigated in syngeneic mice. An intravenous (i.v.) administration of KML-C (20-50 ng/mouse) 2 days before tumor inoculation significantly inhibited lung metastases of both B16-BL6 and colon 26-M3.1 cells. The prophylactic effect of 50 ng/mouse of KML-C on lung metastasis was almost the same with that of 100 $\mu$ g/mouse of KM. Treatment with KML-C 1 day after tumor inoculation induced a significant inhibition of not only the experimental lung metastasis induced by B16-BL6 and colon 26M3.1 cells but also the liver and spleen metastasis of L5178Y-ML25 cells. Furthermore, multiple administration of KML-C given at 3 day-intervals after tumor inoculation led to a significant reduction of lung metastasis and suppression of the growth of B16-BL6 melanoma cells in a spontaneous metastasis model. In an assay for natural killer (NK) cell activity. i.v. administration of KML-C (50 ng/mouse) significantly augmented NK cytotoxicity against Yac-1 tumor cells 2 days after KML-C treatment. In addition, treatment with KML-C (50 ng/mouse) induced tumoricidal activity of peritoneal macrophages against B16-BL6 and 3LL cells. These results suggest that KML-C has an immunomodulating activity to enhance the host defense system against tumors, and that its prophylactic and therapeutic effect on tumor metastasis is associated with the activation of NK cells and macrophages.
Keywords
Korean mistletoe; Lectin; Antitumor activity; NK cell; Macrophages;
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Times Cited By Web Of Science : 24  (Related Records In Web of Science)
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