• Korean Journal of Clinical Laboratory Science
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• v.48 no.4
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• pp.321-326
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• 2016

This study aimed to analyze the changes of C-reactive protein (CRP) and erythrocyte sedimentation rate (ESR) measured in acute infection within four weeks post artificial joint surgery to predict potential infection in early stages, provide rapid treatment, and prevent the abuse of antibiotics. This study included 50 patients with acute infection and 50 patients without any symptoms of infection, among patients who received artificial joint replacement surgery on the lower limbs in a four-week period. CRP was the highest two-to-three days after surgery. with $5.77{\pm}3.69$ and $5.17{\pm}3.48$, respectively, in those with infection and without infection. Thereafter, the value rapidly reduced in those without infection. However, it increased again to $3.16{\pm}2.87$ in the group with infection according to the bimodal curve (p<0.001). ESR was the highest two-to-three days after surgery, with $58.8{\pm}24.63$ and $44.08{\pm}21.48$, respectively. Thereafter, the value slowly reduced in those without infection. However, it was increased again to $47.62{\pm}26.26$ in those with infection according to the bimodal curve p<0.001). As this study shows, if there is an increasing trend for CRP and ESR after artificial joint replacement surgery, it may be possible to question whether patients are acutely infected post surgery. In particular, this result is expected and regarded as a useful factor for diagnosing infection due to the high level of sensitivity and uniqueness for CRP.

• The Korean Journal of Rehabilitation Nursing
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• v.11 no.2
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• pp.99-106
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• 2008

Purpose: The purpose of this study was to identify predictors of obesity, serum lipids and CRP in Korean adults. The predictors of obesity and serum lipids were the subject's general characteristics, life style, eating habit and nutrients. The predictors of CRP were the subject's general characteristics, life style, eating habit, nutrients, obesity and serum lipids. Method: 115 subjects who had visited the health examination center at a hospital participated in the study. The data analysed with descriptive analysis, ANOVA, Chi-square test, Pearson correlation coefficient and multiple regression. Result: Sex, married, eating out ($4{\leq}$/w), eating out (2-3/w) and age ($61{\leq}$) were anticipated variable on BMI ($R^2$=0.488). Sex and overeating (2-3/w) were anticipated variable on body fat($R^2$=0.218). Drink (4-6/w), age (51-60), sex, vegetable fat and Systolic BP were anticipated variable on total cholesterol ($R^2$=0.217). Age (51-60), vegetable fat and unmarried were anticipated variable on LDL ($R^2$=0.180). Sex was anticipated variable on HDL and Triglyceride ($R^2$=0.054, 0.192). Breakfast (1-3/w) and meal (2/d) were anticipated variable on CRP ($R^2$=0.1268). Conclusion: It is thought that decreasing eating out and overeating might be important to prevent obesity. It is thought that decreasing drinking and fat eating might be important to improve serum lipids. It is thought that eating breakfast might be important to decrease CRP.

• Journal of the Mineralogical Society of Korea
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• v.17 no.1
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• pp.49-59
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• 2004

Organic smectite was hydrothermally synthesized by treating the opal-rich siliceous mudstone from the Pohang area with TMAOH solutions and 1:1 solutions of TMAOH+NaOH at $80^{\circ}C$ and concentrations ranging 10∼15%. Smectite was solely formed without accompanying any mineral products in case of TMAOH, whereas NaP and hydroxysodalite was synthesized together with smectite under the blending solution of TMAOH+NaOH. The synthesized smectite is identified as an organic smectite intercalating $TMA^{+}$ within its interlayer site, specifically corresponding to monmorillonite species, through mineralogical characterization by XRD, DTA, and IR analyses. The experimental results indicate that main precursor of the synthesized smectite is undoubtedly opal-CT, and the original sedimentary smectite included as considerable amounts in the mudstone seems to play a major role as Al-sources necessary far the smectite formation. Original inert components such as quartz and mica do not affect mostly to the synthesis reaction, and thus, are resultantly found as impurities in the synthetic products. These experimental results may imply that a new effective method for the low-temperature (less than $100^{\circ}C$) hydrothermal synthesis of organic smectite will be established if some Al-sources adequate for this synthetic system are available.

• Biomedical Science Letters
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• v.4 no.1
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• pp.1-9
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• 1998

A Corynebacterium diphtheriae iron-repressible gene dirA, that was homologous to TSA of Saccharomyces cerevisiae and AhpC subunit of Salmonella typhimurium alkyl hydroperoxide reductase, was amplified with PCR and expressed in E. coli. The DirA purified from the transformed E. coli crude extracts prevented the inactivation of enzyme caused by metal-catalyzed oxidation (MCO) system containing thiols but not by ascorbate/Fe$^{3+}$/$O_2$ MCO system. The DirA concentration, which inhibited the inactivation of glutamine synthetase by 50% (IC$_{50}$) against MCO system, was 0.12 mg/ml. The multimeric forms of DirA were converted to the monomeric form in SDS-PAGE under the thioredoxin system comprised of NADPH, Saccharomyces cerevisiae thioredoxin reductase, and thioredoxin. Also, DirA showed thioredoxin dependent peroxidase activity. All of these results were consistent with the characteristics of a thiol specific antioxidant (TSA) protein having two conserved cysteine residues.

• Korean Journal of Food Science and Technology
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• v.20 no.1
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• pp.106-111
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• 1988

Comparative effects of oxidant, reductant treatment and its phosphorylation on qualities and functional properties of defatted rice bran protein isolates were investigated. Effects of oxidant and reductant treatment were that essential amino acid content of protein isolates was high and its color, pepsin digestibility were good. The phosphorylated defatted rice bran protein isolated was taken by incubating sodium trimeta phosphate in aqueous solution at pH 10.5 and $35^{\circ}C$ for 3 hours and its protein score was 55. Functional properties such as solubility, whipping activity and foam stability were much improved. But color, pepsin digestibility, bulk density and fat absorption were not affected by phosphorylation.

• The Korean Journal of Mycology
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• v.20 no.1
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• pp.72-76
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• 1992

The extraction and separation methods of protein-bound polysaccharides from the mycelium and culture broth of Coriolus versicolor (Fr) Quel were investigated. The use of 2% solution of surface active agent Triton X-100, was effective for extraction of the protein-bound polysaccharides from the mycelium. For the separation and partial purification of the protein-bound polysaccharides, the column chromatography using DEAE-Cellulose and DEAE-Sephadex proved to be effective.

• Journal of the Korean Society of Food Science and Nutrition
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• v.17 no.3
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• pp.233-241
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• 1988

In order to develop a new type of food source for the effective utilization of fish protein, plastein reaction was applied to improve the functional properties of sardine protein. Conditions necessary for optimal plastein productivity from sardine protein using pepsin, ${\alpha}-chymotrypsin$, protease(from Aspergillus saitoi) and papain were established. Sardine protein concentrate was hydrolyzed with pepsin yielding an approximate degree of hydrolysis of 78.4%. Enzyme induced plastein was optimized at : pH 4 for pepsin, pH 7 for ${\alpha}-chymotrypsin$, pH 5 for pretense and pH 6 for papain : Substrate concentrate 40% for pepsin and ${\alpha}-chymotrypsin$, 50% for pretense and papain : the time of incubation, 24hr : enzyme/substrate ratio, 1 : 100(w/v) incubation temperature, $50^{\circ}C$.

• Korean Journal of Food Science and Technology
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• v.32 no.5
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• pp.991-996
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• 2000

Enzyme-linked immunosorbent assay was developed for the analysis of soy protein in foods. Competitive indirect ELISA (ciELISA) was established by using specific antibodies against the heat-stable acidic subunits (AS) of glycinin. Soy proteins in each sample used in this study were solublized in the presence of urea and DTT and boiled at $100^{\circ}C$ for 1hr and then were renatured with a cystine-containing solution. After these treatments, each isolated soy protein (ISP) heated at 60, 70, 80, $90^{\circ}C$ for 10 minutes showed almost the same curve as unheated one in the ciELISA. The detection limit of ISP was 0.3 ${\mu}g/mL$. Anti-AS antibodies have very low reactivities less than 0.1% toward non-meat proteins such as skim milk and casein and did not show any reactivities toward egg white powder and ovalbumin. When laboratory-made sausages containing ISP of $0.5{\sim}3%$ were assayed by ciELISA, the mean recovery was about 83% (C.V., 19%). In addition, the average content of soy protein in commercial sausages was 1.27%.

• 대한예방의학회:학술대회논문집
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• 2005.10a
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• pp.448-448
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• 2005

• Journal of the Korean Orthopaedic Association
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• v.54 no.1
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• pp.30-36
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• 2019

Purpose: This study examined the effects of gender, age, underlying disease, duration after onset of symptoms, preoperative invasive procedures, bacterial culture of joint fluid, and stage of infection by the Gachter classification on the prognosis of patients with infectious knee arthritis who underwent arthroscopic surgery. Materials and Methods: From June 2014 to December 2016, 51 patients who underwent arthroscopic surgery for infective knee arthritis were enrolled in this study. The average follow-up period was 14.2±2.1 months (range, 12-20 months). The subjects were 27 men (52.9%) and 24 women (47.1%), with an average age of 55.1±17.6 years (range, 13-84 years). A preoperative evaluation of the joint aspiration with a count of more than 50,000 leukocytes and a polymorphonuclear leukocyte count of 95% or more was performed. All patients underwent arthroscopic surgery and postoperative continuous joint irrigation. Results: The initial mean value of the C-reactive protein decreased from 9.55±6.76 mg/dl (range, 1.51-31.06 mg/dl) to a final mean of 0.74±1.26 mg/dl (range, 0.08-6.77 mg/dl); the mean duration of C-reactive protein normalization was 27.6±18.9 days (range, 8-93 days). Among the 51 patients who received arthroscopic surgery and antibiotics, 44 patients (86.3%) with infectious knee arthritis completed treatment with improved clinical symptoms, such as fever, pain, and edema, and the C-reactive protein decreased to less than 0.5 mg/dl. Finally, 5 cases were treated with two or more arthroscopic operations, and 2 cases were converted to arthroplasty after prosthesis of antibiotic-loaded acrylic cement. Conclusion: The duration of surgery after the onset of symptoms and the stage according to the Gächter classification are important prognostic factors for predicting the successful treatment of infectious knee arthritis. On the other hand, the other factors were not statistically significant. Nevertheless, patients with bacteria cultured from the joint fluids appear to reflect the treatment period because the period of normalization of the C-reactive protein is shorter than that of the control group.