Korean Journal of Food Science and Technology (한국식품과학회지)
- Volume 32 Issue 5
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- Pages.991-996
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- 2000
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- 0367-6293(pISSN)
An Enzyme-Linked Immunosorbent Assay for Quantitation of Soy Proteins in Food
식품 중 대두단백질의 정량분석을 위한 효소면역측정법
- Shon, Dong-Hwa (Korea Food Research Institute) ;
- Kim, Hyung-Jung (Korea Food Research Institute) ;
- Eum, Byong-Wook (Korea Food Research Institute) ;
- Kim, Soo-Ho (Korea Food Research Institute) ;
- Kim, Soon-Mi (Department of Food & Nutrition, Gachon Gil College)
- Published : 2000.10.31
Abstract
Enzyme-linked immunosorbent assay was developed for the analysis of soy protein in foods. Competitive indirect ELISA (ciELISA) was established by using specific antibodies against the heat-stable acidic subunits (AS) of glycinin. Soy proteins in each sample used in this study were solublized in the presence of urea and DTT and boiled at
식품 중 대두단백질의 분석을 위한 효소면역측정법(ELISA)을 개발하고자 하였다. 대두단백질의 주 구성 성분이며 열에 안정한 glycinin의 acidic subunits(AS)에 대한 특이항체를 생산하였고 이를 이용하여 간접경합 ELISA(ciELISA)를 확립하였다. 시료처리조건은 urea와 DTT로 처리함으로써 용해시킨 다음
Keywords
- anti-soy protein antibodies;
- isolated soy protein (ISP);
- sausage;
- enzyme-linked immunosorbent assay (ELISA)