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Acrosomal Changes and Survival of Following Preservation of Dog Spermatozoa II. Effect of Different Freezing Ramp Rates (개 정자의 보존방법에 따른 첨체 및 생존성의 변화 II. 동결보존에 따른 효과)

  • 정정란;유재규;양성열;여현진;박종식
    • Journal of Embryo Transfer
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    • v.16 no.2
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    • pp.133-138
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    • 2001
  • The aim of this study was to identify the method on extended canine semen exposed to freezing as assessed by motility, survival and acrosomal changes following different freezing ramp rates. Five ejaculates collected by digital manipulation twice weekly from three dogs (Shih-Tzu) were added Tris-Egg Yolk (TE) buffer and divided into 4 aliquots according to formulation of our laboratory. After cooling to 4$^{\circ}C$ by ramp rate of 0.6$^{\circ}C$/min, the samples frozen by ramp rates of 1.6$^{\circ}C$/min to -$25^{\circ}C$, 3$^{\circ}C$/min to -35$^{\circ}C$, 8.9$^{\circ}C$/min to -7$0^{\circ}C$ and 19$^{\circ}C$/min to -11$0^{\circ}C$, respectively, and then stored in L$N_2$for 2days. Each sample was evaluated on their motility, survivability and acrosome integrity at different thawing temperature. The ramp rate of 3$^{\circ}C$/min to -35$^{\circ}C$/h for freezing and thawing temperature of 37$^{\circ}C$ obtained the highest results to improve survivability, motile spermatozoa and intact acrosome appearance than other onditions. In conclusion, we may suggest freezing semen for canine artificial insemination is more efficient with freezing at a ramp rate of -3$^{\circ}C$/min to -35$^{\circ}C$ and thawing with a water bath adjusted to 37$^{\circ}C$.

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A Study on Critical Strain Energy Release Rate Mode II of Chemically Treated SiC-filled Epoxy Composites (표면처리된 탄화규소강화 에폭시 복합재료의 GIIC 특성)

  • Park, Soo-Jin;Oh, Jin-Seok
    • Journal of Adhesion and Interface
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    • v.6 no.4
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    • pp.1-6
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    • 2005
  • In this work, the effect of chemical treatments on surface properties of SiC was investigated in crack resistance properties of SiC/epoxy composites. The surface properties of SiC were determined by acid/base values and FT-IR measurements. Also the crack resistance properties of the composites were studied in critical strain energy release rate mode II ($G_{IIC}$) measurements. As a result, the acidically treated SiC had higher acid value than that of untreated SiC or basically treated SiC. The crack resistance properties of the composites had been improved in the specimens treated by acidic solution. These results were could be attributed to the acide-base intermolecular interaction between SiC and epoxy resin, resulting in increase of the degree of adhesion at interfaces.

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The Results of Postoperative Radiation Therapy in the Rectal Cancer (직장암의 수술 후 방사선치료성적)

  • Lee Kyung-Ja
    • Radiation Oncology Journal
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    • v.12 no.1
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    • pp.91-98
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    • 1994
  • Purpose: Despite apparently complete resection of cancer of the rectum, local recurrence rate was high. Radiation therapy has been used either alone or in combination with chemotherapy as an adjunct to surgery to reduce the risk of recurrence. This study was designed to evaluate the prognostic factors, survival rate and local recurrence rate of the rectal cancer who had received postoperative radiation therapy by retrospective analysis. Method: From 1982 to 1990, 63 patients with cancer of the rectum surgically staged as B2 or C disease received postoperative adjuvant radiation therapy after curative resection of tumor for cure. Postoperative radiation therapy was given to the whole pelvis(mean dose: 5040 cGy in 5-6weets) and perineum was included in irradiated field in case of abdominoperineal resection. Results: Three-year actuarial survival rate was 73.2$ \% $ overall, 87.7$ \% $ in stage B2+3 and 62.9$ \% $ in stage C2+3. Three-year disease-free survival rate was 69.5$ \% $ overall, 87.7$ \% $ in stage B2+3 and 56.8$ \% $ in stage C2+3, Three-year disease-free survival rate in anterior resection was 77.8$ \% $ and 44.4$ \% $ in abdominoperineal resection. The local recurrence rate was 15.9$ \% $ and distant failure rate was 20.6$ \% $. Severe late complication was small bowel obstruction in 6 patients and surgery was required in 4 patients(6.3$ \% $). The prognostic factors were stage(p=0.0221) and method of surgery(p= 0.0414) (anterior resection vs abdominoperineal resection). Conclusion: This study provides evidence supporting the use of postoperative radiation therapy for reducing the local recurrence rate in patients who have had curative resection of rectal cancer with involvement of perirectal fat or regional nodes or both(stage B2 and C).

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Microstructure and Mechanical Properties of Fe-Si-Mn-P High Strength Steel Sheet Controlled by Cooling Rate (냉각제어된 Fe-Si-Mn-P 고장력 강판의 미세조직 및 기계적성질)

  • Moon, Won-Jin;Kim, Ik-Su;Kang, Chang-Yong;Kim, Heon-Ju;Sung, Jang-Hyun;Kim, Ki-Don
    • Journal of the Korean Society for Heat Treatment
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    • v.10 no.2
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    • pp.109-120
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    • 1997
  • Microstructure and mechanical properties of Fe-Si-Mn-P high strength steel sheet have been investigated by controlling the cooling rate. Bainite and ferrite were obtatined by annealing in the ferrite pluse austenite region, and ferrite and austenite were obtatined after annealing in the fully austenite region. Ferrite and pearlite were obtained when the cooling rate was controlled from the annealing temperature above $760^{\circ}C$ and bainite showed with increasing cooling rate, however below $760^{\circ}C$ ferrite and bainite were obtained. Tensile strengths and hardness nearly unchanged with increasing cooling rate after control the cooling rate from the temperature above $760^{\circ}C$, while tensile strengths increased and elongation decreased with increasing cooling rate when the cooling rate was controlled from the tempeature below $760^{\circ}C$. Without regard to annealing temperature, tensile strength increased and elongation decreased with increasing cooling rate. Tensile strengths and elongation values heat treated in the ferrite plus austenite region were higher than those in the fully austenite region. Retained austenite and strength-elongation balance showed the maximum value at $780^{\circ}C$ and decreased with increasing annealing temperature. Strength-elongation balance value was controlled by the retained austenite.

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The Effect of Temperature and Day-Length Conditions on the Growth and Fecundity of Green Rice Leafhopper, Nephotettix cincticeps Uhler (사육온도 및 광조시간이 끝동매미충의 발육과 산란에 미치는 영향)

  • Song Yoo-Han
    • Korean journal of applied entomology
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    • v.17 no.4 s.37
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    • pp.187-191
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    • 1978
  • This experiment was conducted to determine the optimum temperature and day-length condition for laboratory multiplication of Green Rice Leafhopper, Nephotettix cincticeps Uhler, and to obtain basic information for deriving temperature dependent growth rate function. The insects which were reared in $33-35^{\circ}C$ have relatively short nymphal periods and high adult emergence rate compared with the insects which were reared in $27-29^{\circ}C$ The adults which emerged in $35^{\circ}C$ laid significantly smaller number of eggs than the adult reared in $27-29^{\circ}C$ during their nymphal stage. Day length in $29^{\circ}C$ did not have any influence to the nymphal growth, adult emergence rate and egg laying of the insect. The growth rate of the insect did not have linear relation with rearing temperature.

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Reaction of Mast Cells and Goblet Cells in the Small Intestine of C57BL/6 and C3H/HeN Mice Infected with Echinostoma hortense

  • Park Kyeong-Yeol;Lee Kyu-Jae;Kim In-Sik;Yang Eun-Ju;Lim Su-jung;Lim Byung-Hyuk;Ryang Yong-Suk
    • Biomedical Science Letters
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    • v.11 no.3
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    • pp.259-266
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    • 2005
  • Mast cells and goblet cells have been known to protect the host against parasites. In this study, we examined the response of the mast cells and goblet cells over a period of 6 weeks in the duodenum, jejunum and ileum of C3H/HeN and C57BL/6 mice infected with Echinostoma hortense (E. hortense). In addition, we investigated whether the worm recovery rate of uninfected mice (the control group) or E. hortense-infected mice (the experimental group) was associated with the number of mast cells and goblet cells. The worm recovery rate was higher in the C3H/HeN mice than in the C57BL/6 mice. The number of goblet cells significantly increased in the experimental group of the C3H/HeN and C57BL/6 mice compared with the control group of both strains (P<0.005). Worm recovery peaked 3 weeks after the infection of the C57BL/6 mice and at 2 weeks after the infection of the C3H/HeN mice, and it was higher in the duodenum than in the jejunum and ileum. However, the infected site in the intestine had no relation with worm expulsion. In the C3H/HeN and C57BL/6 mice, the number of goblet cells in the experimental group was significantly higher than that in the control group (P<0.005). The number reached a peak 2 weeks after the infection and it even increased in duodenum, jejunum and ileum. The increased number of goblet cells was retained 6 weeks after infection. The number of goblet cells was higher in the C3H/HeN mice than in the C57BL/6 mice (P<0.01). These results indicate that goblet cells are related with the worm expulsion. Furthermore, immunohistostaining of the antral intestinal walls for lectin showed the significant increase of the number of goblet cells in the experimental group (P<0.001). The high infection rate in the duodenum was found during the early infection. An increased infection rate in the jejunum and ileum was found 3 weeks after infection and the infection rate was higher in the C3H/HeN mice than in the C57BL/6 mice. Taken together, the present study indicates that goblet cells, rather than mast cells, may play critical roles in parasite expulsion.

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아세톤을 사용한 목질계 바이오매스의 가용매 분해에 대한 연구

  • Lee, Hong-Seon;Yun, Seong-Uk;Lee, Byeong-Hak
    • 한국생물공학회:학술대회논문집
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    • 2000.11a
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    • pp.216-219
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    • 2000
  • Pyrolysis products of woody biomass were consistedofvarious linear hydrocarbon, aromatics or condensed cyclic compounds. In order to obtain biomass pyrolysis products, more equipments and time were needed. But solvolysis of woody biomass with acetone easily obtained decomposition products and enhanced conversion rate(18.72%, max.) from woody biomass than pyrolysis of woody biomass. Beacause solvolysis with acetone improved conversion rate (26.64%, max.) of lignin. the whole conversion rate was improved. But above $300^{\circ}C$, lignin showed lower conversion rate, so the whole conversion rate decreased. Solvolysis products of woody biomass with acetone were same as pyrolysis products. Above $400^{\circ}C$, methoxy phenols were completely disappeared.

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Fatigue Crack Propagation Characteristics in ASTM 516/60 Steel at Low Temperature (ASTM 516-60 강의 저온피로 크랙전파 특성에 관한 연구)

  • 박경동;김정호
    • Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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    • 2000.10a
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    • pp.240-245
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    • 2000
  • Fatigue crack propagation rates and characteristics of the SA516-60 steel which is used for the low temperature pressure vessels, were studied in the room temperature of $25^{\circ}C$ and low temperature ranges of $10^{\circ}C,\; -10^{\circ}C,\; -30^{\circ}C,\; -50^{\circ}C, \;and\; -70^{\circ}C4 with stress ratio of R=0.05. The obtained experimental results are as follows; 1) In the logarithmic relationship between the fatigue crack propagation rate(da/dN) and stress intensity factor K, the linear relationship was obtained up to da/dN 〉$8\times10^{-3}$/mm/cycle in the same of room temperature, but in low temperature case, the relationship was extended to the range of crack propagation rate. 2) The lower limit stress intensity factor of SA516-60 $\DeltaK_{th}$ was 15.8MPa and in the case of low temperature $-50^{\circ}C\; and\; -70^{\circ}C$, the crack propagation rate da/dN which showed a linear relation, reached rapidly to the $\DeltaK_{th}$/. As the results, the crack propagation rates of $-50^{\circ}C\; and\; -70^{\circ}C$ were lower than that of room temperature and according to the testing temperature the rates were decreased rapidly to the $\DeltaK_{th}$/. 3) On the relationship between the stress intensity factor $\DeltaK$ and the crack propagation cycle, the stress intensity factors of low cycle region was rapidly increased at low temperature, but $\DeltaK$ was increased rapidly at room temperature of high cycle. 4) On the relationship between the fatigue crack propagation rate and cycle, the fatigue crack propagation rate showed higher gradient in the room temperature than the low temperature due to the increment in ductility at low temperature.

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Drying Characteristics of Filefish Fillet (말쥐치육(肉)의 건조특성(乾燥特性))

  • Lee, Byeong-Ho
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.11 no.1
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    • pp.37-43
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    • 1982
  • Constant rate period, falling rate period and diffusion coefficient using filefish fillet as sample during drying in hot air drier were determined under controlled conditions of temperature, humidity and air velocity. Drying rate curve consisted of a short period of constant rate and two stages of falling rate period. When 1 to 3m/see of air velocities were applied, diffusion coefficients were in the range of 1.9130 to $2.6187\;{\times}\;10^{-6}\;\textrm{cm}^2/sec$ at $50^{\circ}C$. 2.4806 to $3.5342\;{\times}\;10^{-6}\;\textrm{cm}^2/sec$ at $60^{\circ}C$ and 4.3405 to $5.3042\;{\times}\;10^{-6}\;\textrm{cm}^2/sec$ at $70^{\circ}C$, respectively. Available lysine content was decreased by 15%. 16% and 20% in the fillet dried at $50^{\circ}C$, $60^{\circ}C$ and $70^{\circ}C$, respectively.

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A Study on Ignitability and Heat Release Rate Characteristics of Rigid Polyurethane Foam (경질 폴리우레탄폼의 착화성 및 열방출특성 연구)

  • 공영건;이두형
    • Fire Science and Engineering
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    • v.17 no.4
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    • pp.117-123
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    • 2003
  • In this study; the ignition and heat release rate characteristics of rigid polyurethane foam were investigated in accordance with setchkin ignition tester and cone calorimeter which is using oxygen consumption principle. In the ignition temperature study; flash-ignition temperature was $383^{\circ}C$-$390^{\circ}C$, self-ignition temperature was$ 493^{\circ}C$∼495$^{\circ}C$. The self-ignition temperature of rigid polyurethane foam was about $100^{\circ}C$ higher than the flash-ignition temperature. In the cone calorimeter study, the time to ignition of rigid polyurethane foam was faster as the external heat flux increase. In the same heat flux level, the time to ignition was faster as the density of rigid polyurethane foam decrease. Also the heat release rate was the largest value at the heat flux of /$50 ㎾\m^2$ and had a tendency of increase as the heat flux level and density increase. In the standpoint of time to ignition and heat release rate, the fire performance of rigid polyurethane foam was influenced by the applied heat flux level and density and the flashover propensity classified by Petrella's proposal was high.