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Studies on Effects of Kinds and Concentration of Cryoprotectants, Equilibration Time and Thawing Temperature on the Survival Rate of Rapidly Frozen Porcine Embryos (돼지 수정란의 급속동결시 내동제의 종류와 농도, 평형시간 및 융해온도에 다른 생존성에 관한 연구)

  • 오원진;오건봉;박병권;김상근;이규승
    • Korean Journal of Animal Reproduction
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    • v.18 no.1
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    • pp.15-23
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    • 1994
  • This study was carried out to investigate the effects of concentration, kinds of cryoprotectants, equilibration time, optimum thawing temperature on the survival rate of rapidly frozen porcine embryos. The porcine embryos following dehydration by cryoprotectants containing sucrose were directly plunged into liquid nitrogen and thawed in 30, 35 or 37$^{\circ}C$ water bath, Survival rate was defined as development rate on in vitro culture or FDA-test. The results are summarized as follows : 1. The high survival rate of porcine frozen embryos after rapidly thawed in freezing medium was attained 2.0M DMSO, 2.0M glycerol, 2.0M propanediol, 1.5M ethyleneglycol. 2. The high survival rate of porcine frozen embryos after rapidly thawed in freezing medium was obtained using single cryoprotectant(16.6~40.0%) than mixed cryoprotectants(12.5~33.3%). 3. The eqilibration time on the survival rate of rapidly thawed porcine frozen embryos was attained after short period of time(15.0~33.3%) in the freezing medium higher than long period of time(9.10~30.0%). 4. The thawing temperature on the survival rate of rapidly thawed porcine frozen embryos was attained at 3$0^{\circ}C$ of thawing temperature(33.3~40.6%) in the freezing medium higher than 25 or 37$^{\circ}C$ of thawing temperature.

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Weed Control by Flame (화염을 이용한 잡초방제 연구)

  • 姜和錫;文學洙
    • Journal of Biosystems Engineering
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    • v.26 no.4
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    • pp.331-336
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    • 2001
  • This study was to develop a kerosene flame weeder. An air compressor was driven though the PTO of a tractor to provide necessary air for fuel combustion and proper pressure to supply fuel from fuel tank to the nozzle. It was found that the flame was extinguished very easily by wind and vibration of the tractor. This trouble could be solved by attaching a burner cap, which is a modified venturi tube, at the end of the nozzle. The constructed flame weeder was tested for the weeding capability in the prepared field. Weed extinction rate and weight decrease rate were analysed. Measured maximum flame temperature was 1,121$\^{C}$ when the fuel consumption was 13.41 kg/h and fuel supply pressure was 88.2 kPa. The maximum temperature occurred at 20cm from the front end the burner, and it decreased to 46$\^{C}$ as the distance increased to 110cm. The flame length of up to 70cm, where the flame temperature was higher than 372$\^{C}$, would be used for weeding purpose. Weed extinction rate and weight decreasing rate increased as the fuel consumption increased. The flame weeder was evaluated to be a practical weeder through improvement as the weed extinction rate and weight decrease rate were analysed to be 75% and 85%, respectively when the fuel consumption was 116.87kg/ha.

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Effect of Loading Rate on the Fracture Behavior of Nuclear Piping Materials Under Cyclic Loading Conditions

  • Kim, Jin Weon;Choi, Myung Rak;Kim, Yun Jae
    • Nuclear Engineering and Technology
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    • v.48 no.6
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    • pp.1376-1386
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    • 2016
  • This study investigated the loading rate effect on the fracture resistance under cyclic loading conditions to understand clearly the fracture behavior of piping materials under seismic conditions. J-R fracture toughness tests were conducted under monotonic and cyclic loading conditions at various displacement rates at room temperature and the operating temperature of nuclear power plants (i.e., $316^{\circ}C$). SA508 Gr.1a low-alloy steel and SA312 TP316 stainless steel piping materials were used for the tests. The fracture resistance under a reversible cyclic load was considerably lower than that under monotonic load regardless of test temperature, material, and loading rate. Under both cyclic and monotonic loading conditions, the fracture behavior of SA312 TP316 stainless steel was independent of the loading rate at both room temperature and $316^{\circ}C$. For SA508 Gr.1a lowalloy steel, the loading rate effect on the fracture behavior was appreciable at $316^{\circ}C$ under cyclic and monotonic loading conditions. However, the loading rate effect diminished when the cyclic load ratio of the load (R) was -1. Thus, it was recognized that the fracture behavior of piping materials, including seismic loading characteristics, can be evaluated when tested under a cyclic load of R = -1 at a quasistatic loading rate.

Economic Feasibility of Automatic Egg Washer (계란 자동세척기의 경제적 타당성 평가)

  • Lin, Qing-Long;Yeo, Jun-Ho
    • Current Research on Agriculture and Life Sciences
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    • v.32 no.3
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    • pp.165-169
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    • 2014
  • This study assessed the economic feasibility of an automatic egg washer. Based on various cost benefit analysis methods, including the net present value (NPV), internal rate of return (IRR) and benefit cost ratio (B/C Ratio), the automatic egg washer was confirmed to have economic feasibility. The NPVs were 38,565,991 won at 5% discount rate and 32,013,916 won at 10% discount rate, respectively. The IRRs were 1.325 at 5% discount rate and 1.299 at 10% discount rate, respectively. Finally, the B/C ratios were 1.726 at 5% discount rate and 1.699 at 10% discount rate.

Genetic Analysis on Germination Rate and Fat Acidity of Japonica Rice Seeds after High Temperature Storage

  • Noh, Gwang-Il;Kim, Ki-Young;Shin, Seo-Ho;Park, Hyun-Su;Kim, Bo-Kyeong;Shin, Mun-Sik;Ko, Jae-Kwon
    • Korean Journal of Breeding Science
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    • v.40 no.3
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    • pp.228-233
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    • 2008
  • This study was carried out to get the genetic information on the germination rate and fat acidity after 12 weeks' storage at $35^{\circ}C$. Germination rate decreased with longer storage period, while fat acidity increased. Germination rate was higher in the order of Koshihikari, Hanmaeum and Unkwangbyeo after 12 weeks' storage at $35^{\circ}C$. Fat acidity of Koshihikari and Hanmaeum was gradually increased, but that of Unkwangbyeo was dramatically increased after 4 weeks' storage at $35^{\circ}C$. The genetic mode on germination rate and fat acidity using the joint scaling test was shown to be additive-dominance gene effects. Additive([d]) effect was higher than dominance effect([h]) in all crosses. The average degree of dominance, [h]/[d], was less than 1, indicating that germination rate and fat acidity was partially dominant. Germination rate and fat acidity based on frequency distribution of 2 crosses were quantitative characters expressed by polygenes.

Spin-up in a Cylinder with a Time-Dependent Rotation Rate (시간에 따라 변화는 회전 각속도를 가지는 원통용기내의 스핀업)

  • Kim, Kyung-Seok;Kwak, Ho-Sang;Hyun, Jae-Min
    • Proceedings of the KSME Conference
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    • 2001.06e
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    • pp.456-462
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    • 2001
  • Comprehensive numerical computations are made of a homogenous spin-up in a cylindrical cavity with a time-dependent rotation rate. Numerical solutions are acquired to the governing axisymmetric cylindrical Navier-Stokes equation. A rotation rate formula is ${\Omega}_f={\Omega}_i+{\Delta}{\Omega}(1-{\exp}(-t/t_c))$. If $t_c$ is large, it implies that a rotation change rate is small. The Ekman number, E, is set to $10^{-4}$ and the aspect ratio, R/H, fixed to I. For a linear spin-up(${\epsilon}<<$), the major contributor to spin-up in the interior is not viscous-diffusion term but inviscid term, especially Coriolis term, though $t_c$ is very large. The viscous-diffusion term only works near sidewall. But for spin-up from rest, when $t_c$ is very large, viscous-diffusion term affects interior area as well as sidewall, initially. So azimuthal velocity of interior for large $t_c$ appears faster than that of interior for relatively small $t_c$. However, the viscous-diffusion term of interior decreases as time increases. Instead, inviscid term appears in the interior.

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Erosion properties of plasma sprayed zirconia Based coatings (지르코니아계 용사 코팅층의 Erosion 특성)

  • 신종한;임상규;임대순
    • Proceedings of the Korean Society of Tribologists and Lubrication Engineers Conference
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    • 2001.06a
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    • pp.346-353
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    • 2001
  • Zirconia powder containing 3 mol% yttria(3Y-PSZ) with and with out Fe$_2$O$_3$ addition was coated on tile cast iron substrate by plasma spraying method. The erosion experiments were performed at temperatures from $25^{\circ}C$ to $600^{\circ}C$. A gas blast type erosion tester was used to examine erosion behavior of the specimens. The results of 3Y-PSZ coatings showed that tile erosion rate had maximum value at 40$0^{\circ}C$. It coincided with tile results of phase transformation tetragonal phase to monoclinic phase caused by low temperature thermal degradation. The tensile stress relaxation and the micro-hardness improvement significantly influenced on the erosion rate at $600^{\circ}C$. In the case of Fe$_2$O$_3$ added 3Y-PSZ coatings, the erosion rate of tested at $25^{\circ}C$ showed maximum value at 5.0 mol% Fe$_2$O$_3$ added coating. This tendency is caused by the improvement of mechanical properties and the tensile residual stress. The erosion rate at 200'c and 400'L showed significantly decrease by Fe203 addition. This decrease is believed to be the stabilization of the tetragonal phase and the increase of micro-hardness.

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Lignocellulose Biodegradation and Interaction between Cellulose and Lignin under Sulfate Reducing Conditions (황산염 환원 조건에서 리그노셀룰로오스의 분해 및 리그닌과 셀룰로오스의 상호작용)

  • Ko, Jae-Jung;Kim, Seog-Ku;Shimizu, Yoshihisa
    • Journal of the Korea Organic Resources Recycling Association
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    • v.15 no.4
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    • pp.131-137
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    • 2007
  • In this study, the biodegradation test on lignocellulose under sulfate reducing conditions was carried out. In particular, the interaction between cellulose and lignin was investigated with various g-cellulose/g-lignin (C/L) ratios: 42.15, 4.59, 2.51, 1.14 and 0.7. It was shown that the rate of cellulose degradation decreased in proportion to the lignin content. Assuming first order degradation kinetics, the consequences of competitive inhibition were graphically shown for different C/L ratios. The relation between cellulose reduction rate and C/L ratio was expressed by logarithm function with a determination coefficient of 0.97. Lignocellulose reduction rate was also described as a logarithm function of C/L ratio showing a inhibition effect by lignin. In the mean time, the rate of lignin decomposition was higher at C/L ratio of 2.51 and 1.14 compared with C/L ratios of 4.59 and 0.7, indicating that excessive extra carbon source is not appropriate for lignin biodegradation.

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Survival Rate and Hematological Responses with Temperature Changes of Red Spotted Grouper, Epinephelus akaara in South Korea

  • Park, Jong Youn;Han, Kyeong Ho;Cho, Jae Kwon;Kim, Kyong Min;Son, Maeng Hyun;Park, Jae Min;Kang, Hee Woong
    • Development and Reproduction
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    • v.20 no.2
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    • pp.81-90
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    • 2016
  • The effect of sudden changes of water temperature (WT) on the survival rate and physiological responses of the red spotted grouper (Epinephelus akaara) were examined by manipulating WT control system for 9 days. Experimental condition was divided in two different regimes at low (from $10^{\circ}C$ to $4^{\circ}C$, decreased $1^{\circ}C/d$) and high (from $28^{\circ}C$ to $34^{\circ}C$, increased $1^{\circ}C/d$) WT. Survival rate of experimental fishes were observed, and determined the changes of hematological characteristics by analyzing plasma levels of cortisol, glucose, total protein, and electrolytes ($Na^+$, $Cl^-$, $K^+$). No mortality was observed until low WT $6^{\circ}C$ (144 h) and high WT $32^{\circ}C$ (96 h), and 100% mortality was observed at low WT $4^{\circ}C$ (216 h) and high WT $35^{\circ}C$ (171 h). Plasma levels of cortisol and glucose increased rapidly as decreasing WT, and the loss of swimming ability and respiration response was observed at low WT $7^{\circ}C$ and high WT $34^{\circ}C$ conditions.

Effect of Temperature on Hatching Rate of Triops longicaudatus (Triopsidae, Notostraca) (온도가 긴꼬리투구새우(Triops longicaudatus (LeConte): Triopsidae, Notostraca)의 부화에 미치는 영향)

  • Kwon, Soon-Jik;Kwon, Hyeok-Young;Jun, Yung-Chul;Lee, Jong-Eun;Won, Doo-Hee
    • Korean Journal of Ecology and Environment
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    • v.42 no.1
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    • pp.32-38
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    • 2009
  • The hatching rate of the eggs of Triops longicaudatus (LeConte), an endangered tadpole shrimp species in Korea, was experimented in different levels of temperature (10, 15, 20, 25, 30, 35, 40, 45, and $50^{\circ}C$) under 24 L photoperiod conditions in incubator. Eggs of T. longicaudatus in this study were obtained from the adults collected from Andong-si, Gyeongsangbuk-do, Korea, in July 2006. The highest hatching rate was the 80.0 (${\pm}9.43$)% in average at $35^{\circ}C$. The thermal threshold for hatching was derived at $6.66^{\circ}C$. However, eggs were not hatched both at $10^{\circ}C$ and at $50^{\circ}C$ during the experimental period.