• Journal of Microbiology and Biotechnology
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• v.12 no.3
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• pp.508-513
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• 2002

Human endogenous retroviral long terminal repeats (LTRs) have been found to be coexpressed with sequences of genes located nearby. It has been suggested that the LTR elements have contributed to the structural change or genetic variation of human genome connected to various diseases. The HERV-W family has been identified in the cerebrospinal fluids and brains of individuals with schizophrenia. Using a cDNA library derived from a human brain, the HERV-W LTR elements were examined and five new LTR elements were identified. These elements were examined using a YAC clone panel from the Xq21.3 region linked to psychosis that was replicated on the Y chromosome after the separation of the chimpanzee and human lineages. Fourteen elements of the HERV-W LTR were identified in that region. Those LTR elements showed a high degree of sequence similarity ($91.8-99.5\%$) with previously reported HERV-W LTR. A phylogenetic tree obtained from the neighbor-joining method revealed that new HERV-W LTR elements were closely related to the AXt000960, AF072504, and AF072506 from the GenBank database. The data indicates that several copy numbers of the HERV-W LTR elements exist on the Xq21.3 region and are also expressed in the human brain. These LTR elements need to be further investigated as potential leads to neuropsychiatric diseases.

• Korean Journal of Cognitive Science
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• v.1 no.2
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• pp.361-376
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• 1989

Norepoine is N-methylated by the enzyme phenly ethanolamine N-metyltransferase(PNMT)to form epinephrine.this enzyme is larhly restructed to the adrenal medulla where epinephrine in mammalian brain where epinephrine function as a neurotransmitter.It seems clear that central epinephrine is involved in the regulation of cardiovacular function and in several forms of hypertension.However,information about the struture of mammalian epinephrine forming enzyme has been limited until now.But recently we isolate bovine and human PNMT cDNA clone using gtll expression library and sequcde total nucleotide composition.To obtain information about the structrue of the human and rat PNMT proteins and gones and to further define the extent of the evolutionary relationships among the PNMT molecules of these species human and rat genomic DNA clones to PNMT were sequentially isolated and characterized.

• Fisheries and Aquatic Sciences
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• v.12 no.4
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• pp.286-292
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• 2009

We have constructed a cDNA library using brain samples of olive flounder Paralichthys olivaceus. Here, we described the study on gene identification by screening 356 clones from the brain cDNA library of olive flounder. Here, we screened 356 clones from the library to identify genes. Of these, 176 (49.5%) were identified as orthologs of known genes from olive flounder and other organisms. Among the 176 EST clones, 33 (18.7%) represented 11 unique genes that are identical to expressed sequence tags (ESTs) reported for olive flounder, and 120 (68.2%) represented 102 unique genes known from other organisms. The percentage of unknown genes (50.5%) is higher than in other olive flounder cDNA libraries (Lee et al., 2003, 2006, 2007), reflecting the high complexity of brain tissue. Further studies of expression characterization and developmental behavior related to these genes should provide useful insight into the physiological functions of the brain in olive flounder.

• Korean Journal of Computational Design and Engineering
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• v.9 no.4
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• pp.373-381
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• 2004

Using standard components of a press die is recognized as a way for a cost reduction and a short lead time. It also provides a way for a quick maintenance of a die under repair. It is expected to contribute to integration of CAD/CAM system for manufacturing dies in the future. This paper presents a 3D CAD library which is constructed using the standard components and is used for designing a press die. This 3D CAD library is generated by a database system made of Microsoft Access for standard components and by CATIA V5 R10 API for geometric features. The library is implemented using Visual Basic 6.0 utility of CATIA API function in the Windows NT environment. It creates a 3D model of the standard components of press die easily when a die designer inputs numerical values of geometric features and the BOM of the completely assembled parts. It also generates automatically the assembly drawing of die set by using variables for standard values of die parts. Therefore users can save the cost of time to design the press die components, and even a beginner can use this program with ease. The test results of the 3D CAD library for designing shearing and bending dies verify its usefulness and feasibility.

• Journal of The Korean Digital Architecture Interior Association
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• v.2 no.1
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• pp.16-23
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• 2002

The Subject of this research is building integrated library to supported to CAAD of the A.E.C field that is oriented to the environment of integrated design. In this research, we design and implement a new library model as a solution of design-data sharing and multi-displaying that is on the rise among many problems in construction integration. We name this integrated library ACO(Actual Component Object) and using the ACO, we can share data without loss in the fields such as construction, estimation, structure. Together with this, the different expressions along each field provide the solution of sharing both graphic data and non-graphic data. This ACO is a newly conceptualized Library that is able to supply a composite structure to an architect(designer) through the internet-based ACO System. To the ACO System that supplies those ACO service, we introduce the concept of DBMS in design and design administrator, and we investigate the solutions that enable an architect to use this ACO directly on the Web.

• Journal of the Korean Society for information Management
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• v.31 no.2
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• pp.189-209
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• 2014

This study examined librarians' perceptions of Information Commons(I.C.), user's information seeking behaviors, and new role of library in the digital ages. Interviews with 28 librarians found that the perceptions of the librarians were widely differed depending on their understandings of the nature of the space. The interview results were divided into three different categories of librarians: the librarians; 1) understanding library as a place only with academic functions, 2) library as academic place along with multi-cultural functions, and 3) library as open learning space with focus on creativity and discovery of users. The findings also indicated that all these perceptions are closely related to understanding of the role of library and its future development.

• Journal of the Korean Society for Library and Information Science
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• v.12
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• pp.39-86
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• 1985

The main purpose of the present study is to survey the major iudexing bulletins of national nature in Korea, to define such problem areas as lacunae, duplicates and limitation in coverage in the indexing services currently available in Korea, and to make some suggestions for action for improving the existing indexing services in the light of general principles and the tradition and constraints unique to Korea. The major findings and conclusions reached at this study are summarised as follows: (A) A new indexing bulletin of general nature covering the entire field needs to be created in each of the following fields without an established indexing service available for the outcome of research and development activities in Korea. (1) Philosophy (2) Religion (3) Pure sciences (4) Art (5) Language (6) Literature (7) History (B) A new specialised indexing bulletin needs to be created in each of the following fields where indexing services are heavily utilised but no, or only partial, indexing service is available. (1) Social sciences (a) Statistics (b) Sociology (c) Folklore (d) Military science (2) Pure sciences (a) Mathematics (b) Physics (c) Chemistry (d) Astronomy (e) Geology (f) Mineralogy (g) Life sciences (h) Botany (i) Zoology (3) Applied sciences (a) Medicine (b) Agriculture (c) Civil engineering (d) Architectural engineering (e) Mechanical engineering (f) Electrical engineering (g) Chemical engineering (h) Domestic science (C) Publication of the indexing bulletins suggested in A and B above may be ideally carried on by a qualified and dependable learned society established in the respective fields and designated by the Minister of Education, and should be financially supported from the public fund under the provisions of Art. 27 of the Scientific Research Promotion Act of 1979. (D) The coverage and contents of the four indexing bulletins in the field of banking and financing published by the Library of the Bank of Korea are similar and considerably duplicated. It is, therefore, suggested that the four indexing bulletins are combined in one to form a more comprehensive and efficient bibliographical tool in the field and it is further developed into a general guide to the literature produced in the entire field of economics in Korea by gradually expanding its subject coverage. (E) For the similar reasons stated in D, the Index to the Articles on North Korea and the Catalogue of Theses on North Korea, both publisheds by the Ministry of Unification Library, are suggested to make into one. The Index to the Articles of the Selected North Korean Journals and the Index to the Articles of the North Korean Journals in Microfilm Housed in the Ministry of Unification Library, both published by the same Library, are also suggested to be combined in one. (F) The contents of the Catalogue of the Reports Submitted by Government Officials Who Have Travelled Abroad, published by the National Archives are included in the Index to the Information Materials Related to Government Administration, published by the National Archives. The publication of the former is hardly justified. (G) The contents of the Index to Legal Literature published by the Seoul National University Libraries and those of the Law Section of the Index to Scholastic Works published by the National Central Library are nearly identical. One of the two indexes should cease to be published. (H) Though five indexes are being published in the field of political science and four in the field of public administration, their subject coverage is limited. Naturally, these indexes are little usable to many other researchers in the two fields. A comprehensive index covering all the specialised areas in each field needs to be developed on one or all the existing indexes. (I) It is suggested that the Catalogue of the Scholastic Works on Curricula published by the National Central Library expands its subject coverage to become a more usable and effective index to all the researchers in the field of education. (J) The bimonthly Index to Periodical Articles and the specialised index by subject series published by the National Assembly Library, and the Index to Scholastic Works published by the National Central Library are expected to increase their coverage and frequency of publication to be used more effectively and more efficiently by all users in all fields till the indexing bulletins suggested in this study will fully be available in Korea.

• Korean Journal of Agricultural Science
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• v.44 no.4
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• pp.495-503
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• 2017

Genotyping-by-sequencing (GBS) is an outstanding technology for genotyping and single nucleotide polymorphism (SNP) discovery compared to next generation sequencing (NGS) because it can save time when analyzing large-scale samples and carries a low cost per sample. Recently, studies using GBS have been conducted on major crops and, to a greater extent, on fruit crops. However, many researchers have some problems due to low GBS efficiency resulting from low quality GBS libraries. To overcome this limitation, we developed an efficient GBS library construction method that regulates important conditions such as restriction enzymes (RE) digestion and a PCR procedure for grapevine. For RE digestion, DNA samples are digested with ApeKI (3.6U) at $75^{\circ}C$ for 5 hours and adapters are ligated to the ends of gDNA products. To produce suitable PCR fragments for sequencing, we modified the PCR amplification conditions; temperature cycling consisted of $72^{\circ}C$ (5 min), $98^{\circ}C$ (30 s), followed by 16 cycles of $98^{\circ}C$ (30 s), $65^{\circ}C$ (30 s), $72^{\circ}C$ (20 s) with a final extension step. As a result, we had obtained optimal library construct sizes (200 to 400 bp) for GBS analysis. Furthermore, it not only increased the mapping efficiency by approximately 10.17% compared to the previous method, but also produced mapped reads which were distributed equally on the19 chromosomes in the grape genome. Therefore, we suggest that this system can be used for various fruit crops and is expected to increase the efficiency of various genomic analysis performed.

• Korean Journal of Poultry Science
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• v.33 no.4
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• pp.249-254
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• 2006

This experiment was conducted to examine the effective DNA related with muscle growth of Korean native chicken. cDNA library was constructed with mRNA subtraction from Korean native chicken to Cornish. Total mRNA was purified from pectoralis muscle of adult chicken. Five clones were compared their DNA sequence and characteristics based on GenBank. Clone NDS-1 (618nt) was low homology (10%) with other species, but it is closely related with triosephosphate isomerase which is play an important role in glycolysis. Clone NDS-6 (651nt) is corresponding to glyceraldehyde-3-phosphate dehydrogenase. These two clones are encoding to enzymes in key role in glycolysis. However, other three clones (NDS-2, NDS-10, NDS-12) have low homology with other species about 5.0%. These clones were not similar with any other eukaryotics. Therefore, three clones (NDS-2, NDS-10, NDS-12) are high possibility of specific DNA for muscle growth in Korean native chicken.

• Journal of the Korean Society for Library and Information Science
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• v.10
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• pp.3-37
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• 1983

1. Library practice education in Korea is in the form of field experience, that is, Practicum, which is one type of field experience. 2. The practicum is a short-term work experience guided by the academic institutions. (2 to 6 weeks of full time employment in a library, or one to two semesters of approximately 10 to 12 hours weekly) 3. Field work is largely accepted as a required subject. 4. Field work is usually carried out during the first semester of senior year. (or of sophomore year in junior college) 5. Libraries in other universities (or in other institutions) can beused for a student's field work. But the current tendency is to use his or her own university's library. 6. Most universities give one credit for 2 weeks' field work. 7. The method of evaluation is strict: A or 'excellent' (above 90), B or 'good' (above 80), C or 'fair' (above 70), D or 'bad' (above 60). 8. The expense of field work is usually included in the tuition fees. However, some think that an additional fees should be collected in the case of special practice. 9. Respondents to my survey say that the field experience is necessary so that professional librarians can connect theory and practice. 10. They also indicate that there are some problems in library practice education: administrative difficulties in the department chairman's office, the burden on professors in the library being used, and the limited number of libraries in which the field work is practicable. 11. The Department of Library Science is responsible primarily for providing faculty supervision over the students, and secondarily for the preparation of evaluation cards and field work reports. 12. Most of the faculty respondents use evaluation cards for the field work, some of which serve as grade sheets, some as field work diaries, and some as field work reports.