• The Korean Journal of Nuclear Medicine
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• v.33 no.3
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• pp.316-326
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• 1999

Purpose: We evaluated the feasibility of extracting pure left ventricular blood pool and myocardial time-activity curves (TACs) and of generating factor images from human dynamic N-13 ammonia PET using factor analysis. The myocardial blood flow (MBF) estimates obtained with factor analysis were compared with those obtained with the user drawn region-of-interest (ROI) method. Materials and Methods: Stress and rest N-13 ammonia cardiac PET imaging was acquired for 23 min in 5 patients with coronary artery disease using GE Advance tomograph. Factor analysis generated physiological TACs and factor images using the normalized TACs from each dixel. Four steps were involved in this algorithm: (a) data preprocessing; (b) principal component analysis; (c) oblique rotation with positivity constraints; (d) factor image computation. Area under curves and MBF estimated using the two compartment N-13 ammonia model were used to validate the accuracy of the factor analysis generated physiological TACs. The MBF estimated by factor analysis was compared to the values estimated by using the ROI method. Results: MBF values obtained by factor analysis were linearly correlated with MBF obtained by the ROI method (slope = 0.84, r = 0.91), Left ventricular blood pool TACs obtained by the two methods agreed well (Area under curve ratio: 1.02 ($0{\sim}1min$), 0.98 ($0{\sim}2min$), 0.86 ($1{\sim}2min$)). Conclusion: The results of this study demonstrates that MBF can be measured accurately and noninvasively with dynamic N-13 ammonia PET imaging and factor analysis. This method is simple and accurate, and can measure MBF without blood sampling, ROI definition or spillover correction.

• Korean Journal of Organic Agriculture
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• v.18 no.3
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• pp.387-399
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• 2010

This experiment was conducted to investigate the effects of dietary supplementation of oriental herb extracts (Alpha $Plus^{(R)}$ (AP)+Dried Foxglove Powder (DFP)) on growth performance and proliferation of splenocytes in broiler chicken raised under low or high density stocking conditions. A total of 240 male broiler chicks were randomly divided into four treatment groups with three replicates composed of ten birds in low density stocking groups and thirty birds in high density stocking groups. The four treatment groups were as follows: Cl (low density without AP and DFP); T1 (low density with AP 0.2% and DFP 0.03%); C2 (high density without AP and DFP); T2 (high density with AP 0.2% and DFP 0.03%). The feed intake of the high density stocking group was significantly lower than that of low density stocking groups during overall experimental period (p<0.05). The body weight of high density stocking groups was lower than that of low density stocking groups (p<0.05). The decrease of feed intake and body weight gain in growing stage (7~25day) under the high density stocking condition seems to be recovered in final stage (26~34day). Meanwhile dietary supplementation of AP+DFP in low and high density stocking groups tended to have higher daily body weight gain than control groups. The proliferation of splenocytes in treatment groups was significantly higher than control groups (p<0.05). In conclusion, the result of the present study suggested that the oriental herb extracts (AP+DFP) could be used as an alternative feed additive resources to improve growth performance and immune activity in broiler chickens.

• Korean Journal of Soil Science and Fertilizer
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• v.30 no.4
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• pp.361-369
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• 1997

A microbiological nitrate determination method by E. coli is modified in Korea, using K12 wildtype, KCTC 1116, for the quantitative reduction of $NO_3{^-}$ to $NO_2{^-}$. The nitrate in plant, soil or water sample is determined spectrophotometrically after being diazotized with sulfaniamide and N-(1-naphthl)-ethlenediamine. The modified E. coli cell method and principle for nitrate determination using Korean wildtype E. coli strain is described, and cell culture and preparation of stock suspension for E. coli as well. This modified E. coli cell method can be managed simply and fast, it is suitable for the investigation of the large serials, it can be also automated and has a high degree of sensitivity up to 0.01ppm $NO_3{^-}-N$ in the sample solution. The applicability of the modified E. coli cell method has been tested for plant, soil and water analysis on a wide range of different samples. Recovery rates of added nitrate have been determined and comparisons with other standard nitrate analytical procedures have been carried out. The results with the modified E. coli cell method show high correlation ($r^2=0.98$) with those gained by the standard analytical procedures. The advantages and disadvantages of the method are also discussed to other nitrate determination methods.

• Journal of Dairy Science and Biotechnology
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• v.27 no.2
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• pp.7-16
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• 2009

Appenzeller cheese samples were prepared by addition of 0.5, 1.0, and 2.0% green tea (Camellia sinensis, CS) powder and control cheese. We examined various quality characteristics of the novel cheese, such as viable-cell counts, pH, water-soluble nitrogen (WSN), non-casein nitrogen (NCN), non-protein nitrogen (NPN), and catechin level during maturation for 16 weeks at $14^{\circ}C$. To develop a Korean natural cheese containing green tea powder, we also analyzed the changes in the polyacrylamide gel electrophoresis pattern, chemical composition, and sensory qualities. The viable cell counts of the samples were not significantly different. Until the $3^{rd}$ week, the pH of the CS cheese decreased with an increase in the maturation time. However, the pH gradually increased by the $12^{th}$ week, while WSN, NCN, NPN also increased. The WSN, NCN, NPN, and catechin values for the CS cheese samples were significantly higher than the values for the control cheese. The polyacrylamide gel electrophoretic pattern of caseins for the CS cheese indicated that this cheese degraded more rapidly than the control cheese did. In the sensory evaluation, cheese with 1.0% CS powder showed the highest scores in taste and appearance and good scores in flavor and texture. These results indicate that 1.0% CS is the optimal value for addition to cheese, and cheese containing 1.0% CS shows good physiological properties and reasonably high overall sensory acceptability.

• Korean Journal of Environmental Agriculture
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• v.13 no.2
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• pp.223-230
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• 1994

A practical study on a drying and fermentation system equipped with a stirring machine operated mechanically, of pig manure was conducted to prove the efficiency of and practicability to an ordinary pig farm. The type of the drying bed was a round-shaped (r=3m) concrete structure and the stirring machine was adopted to stir and transfer dried pig manure to the fermentation tank. The dried pig manure was put into a fermentation tank ($V=18m^3$), which was aerated from pipe lines installed at the bottom. While water content of pig manure passing through a drying bed was remarkably reduced than before drying, the drying efficiency of this system decreased in winter. However, the temperature of pig manure piled up in the fermentation room in winter reached over $60^{\circ}C$ and excess water of pig manure was removed during the fermentation process. The reduction rate of water content of pig manure, to which dried pig manure was added as bulking material on the drying bed, was 52.1%, but when dried without bulking material it was only 19.7%. Although the content of $P_2O_5$ of dried pig manure was slightly higher than that of fresh pig manure, progressive changes in chemical composition between fresh and dried pig manure made no great difference. Among the contents of minerals of fresh and dried pig manure, CaO was the highest and the rest were in the decreasing order of $K_2O$, MgO, and $Na_2O$. Population density of E. coli and Streptococci of dried pig manure was reduced by 142 and 236 times that of fresh pig manure, respectively. The installation cost of this drying and fermentation system was 4,185,630 won (approximately 5,232 US $) and operating cost per year was 190,000 won (237.5US $) on the basis of self-labor condition.

• Korean Journal of Clinical Laboratory Science
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• v.50 no.1
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• pp.37-43
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• 2018

Chamaecyparis obtusa (CO) has recently been attracting attention because of its beneficial effects on skin allergies, atopic dermatitis, and skin diseases, such as acne and eczema. In the present study, the extract from CO leaf grown in Jangseong gun, Jeollanam-do, Korea was evaluated for its anti-oxidant, anti-inflammatory, and anti-allergic effects in vitro. The total polyphenol content of the CO leaf extract was $25.89{\pm}0.31mg$ gallic acid equivalents (GAE)/g. Gas-chromatography mass-spectrometry (GC-MS) analysis revealed the presence of six compounds in the CO leaf extract: ${\alpha}-terpinene$ (3.03 mg/g), ${\alpha}-terpineol$ (9.48 mg/g), limonene (5.96 mg/g), borneol (59.78 mg/g), myrcene (4.85 mg/g), and sabinene (11.31 mg/g). The $RC_{50}$ values of the CO leaf extract for $H_2O_2$ and ABTS radical were $5.47{\pm}0.13mg/mL$ and $4.00{\pm}0.01mg/mL$, respectively. In addition, the CO leaf extract showed significant inhibitory effects on lipopolysaccharide-induced nitric oxide production in RAW 264.7 cells and IgE-induced release of ${\beta}-hexosaminidase$ (degranulation) in mast-cell like RBL-2H3 cells. The cell viability assay showed that the CO leaf extract ($100{\sim}800{\mu}g/mL$) did not affect the viability of human normal skin fibroblast CCD-986sk cells significantly. Overall, these results suggest that the CO leaf extract is a potential functional cosmetic ingredient that can exert anti-oxidant, anti-inflammatory, and anti-allergic effects.

• Journal of The Korean Society of Grassland and Forage Science
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• v.23 no.1
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• pp.65-70
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• 2003

This study was conducted to select the promising varieties of alfalfa (va.) Venal, Ancho., P5444, DK120, DK125, Horizon, DK135, Luna and Kitawacaba at Daekwanryong branch(altitude 800m a.s.l.) and Namwon branch(altitude 450m a.5.1.) of National Livestock Research Institute Leaf width of alfalfa was broad in all varieties except fer Vernal, and DK125 was flowered the earliest of all varieties. Plant height of Luna was the longest, and winter hardness of P5444 was the greatest with 80.2% of all varieties. In Daekwanryong, dry matter(DM) yield of Luna was the highest and DM yield of vernal was the lowest among the varieties in Namwon. In both areas, dry matter yield ef P5444 was the highest of alfalfa varieties. In Daekwanryong, ADF and NDF concentrations of DK 120 were the lowest of all alfalfa varieties. In Namwon, ADF concentrations of Horizon was the lowest with 26％, and NDF concentrations of DK 125 and Horizon was the lowest with 40.8%. Crude protein concentrations of P5444, Luna and DK 120 were higher than that of different varieties in Daekwanryong and crude protein content of Horizon was the highest with 28.1% in Namwon. Average crude protein yield of both areas was the highest of P5444. The results of this study indicated that Luna, Horizon and P5444 would be the promising alfalfa varieties in Daekwanryong, Namwon and both areas, respectively.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.5
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• pp.761-767
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• 2007

This study was conducted to determine the effects of exogenous zinc-metallothionein (Zn-MT) on anti-oxidative function and pork quality. After feeding a corn-soybean meal-based diet for two weeks, 48 pigs ($Duroc{\times}Landrace{\times}Chinese\;Black Pig$) were assigned randomly to four groups. Pigs in Group 1 were maintained under non-stress conditions, whereas pigs in Groups 2, 3 and 4 were aggressively handled for 25 min to produce stress. Pigs in Groups 1, 2, 3, and 4 received intramuscular administration of saline (control group; CON), 0 (negative control group; NCON), 0.8 (low dose group; LOW), and 1.6 (high dose group; HIGH) mg rabbit liver Zn-MT per kg body weight, respectively. Pigs were slaughtered at 3 and 6 h post-injection. Zn-MT treatment increased (p<0.05) the activities of superoxide dismutase (SOD) and glutathione-peroxidase (GSH-PX) while decreasing the concentration of malondialdehyde (MDA) in liver. These responses were greater (p<0.05) at 6 h than at 3 h post Zn-MT injection. Zn-MT treatment increased (p<0.05) hepatic SOD mRNA levels in a time and dose-dependent manner and decreased (p<0.05) serum glutamate-pyruvate transaminase and lactate dehydrogenase activities (indicators of tissue integrity). Zn-MT administration decreased (p<0.05) lactate concentration and increased (p<0.05) pH and water-holding capacity in the longissimus thorasis meat. Collectively, our results indicate that intramuscular administration of Zn-MT to pre-slaughter stressed pigs improved tissue anti-oxidative ability and meat quality.

• The Korean Journal of Physiology
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• v.8 no.1
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• pp.27-30
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• 1974

It was planned to evaluate the influence of Panax Ginseng upon hepatic DNA synthesis in mice by observing incorporation of $[^3H]$ thymidine into the tissue cells. Thirty male mice$(body\;weight:\;18{\sim}20\;g)$ were divided equally into the ginseng and the saline groups. Each animal of the ginseng and the saline groups received every day (subcutaneously) 0.05 m1/10 g body weight of ginseng extract (4mg of ginseng alcohol extract in 1 ml of saline) and the same amount of saline, respectively, for 5 days. On the 5th experimental day, all animals received $1\;{\mu}Ci/g$ body weight of $[^3H]$ thymidine intraperitoneally 2 hours after the last medication. Five animals, at a lime, of each group were sacrificed 1, 10, and 24 hours after thymidine administration, and their hepatic radioactivity was measured autoradiographically in terms of the % number of radioactive cells in 1,000 cell counts (Radioactive Index, R.I.). Following results were obtained: 1. The hepatic radioactive indices obtained from the saline group 1, 10, and 24 hour after $[^3H]$ thymidine administration were $3.23{\pm}0.23,\;5.20{\pm}0.21,\;and\;6.00{\pm}0.30\;(mean{\pm}S.D.)$, respectively. 2. The corresponding values obtained from the ginseng group $(4.22{\pm}0.33,\;6.32{\pm}0.32,\;and\;7.42{\pm}0.35)$ were significant higher than the values of the saline group. The inference from the above results was that the ginseng facilitated hepatic DNA synthesis.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.35 no.3
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• pp.302-307
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• 2002

To investigate difference of components changes in salt-fermented anchovy, Engaulis japonicus sauce during 18 months fermentation by tank size, various chemical properties were examined at 2$\~$3 months intervals. The contents of total and amino nitrogen, total ATP related compounds increased gradually during 18 months of fermentation, and showed higher content in salt-fermented anchor sauce produced by large tank scale (LTS) product than those of small tank scale (STS) product during fermentation. Hypoxanthine and uric acid were the most abundant in ATP related compounds, ranging from $81.1\%$ to $90.4\%$, The cross point of inosine (HxR) + hypoxanthine (Hx) and uric acid was faster in LTS with 10.3 months fermentation than in STS with 12.6 months fermentation. After 18month of fermentation, the LTS was rich in free amino acids, such as glutamic acid, alanine, aspartic acid, valine, Iysine in that order. On the other hand, the STS was rich in free amino acids, glutamic acid, aspartic acid, alanine, vsine, valine in that order. Absorbance at 453 nm were higher in STS than in STS, but was no difference the rate of increase during fermentation.